BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0474 (656 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar... 135 5e-33 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 60 3e-10 SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 55 8e-09 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 54 1e-08 SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo... 51 1e-07 SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 45 8e-06 SPBC32H8.10 |cdk9||cyclin-dependent protein kinase Cdk9 |Schizos... 34 0.021 SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 31 0.15 SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces po... 30 0.26 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 30 0.26 SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 29 0.59 SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces ... 29 0.59 SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizo... 28 1.4 SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 27 1.8 SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 2.4 SPAC2C4.14c |ppk11||PAK-related kinase Ppk11|Schizosaccharomyces... 27 2.4 SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 27 3.1 SPAC24B11.11c |sid2||Sid2p-Mob1p kinase complex|Schizosaccharomy... 26 5.5 SPAC688.06c |slx4||structure-specific endonuclease subunit |Schi... 26 5.5 SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|... 25 7.3 SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosa... 25 7.3 SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 25 9.6 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 25 9.6 SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 25 9.6 >SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 544 Score = 135 bits (327), Expect = 5e-33 Identities = 68/122 (55%), Positives = 85/122 (69%), Gaps = 1/122 (0%) Frame = +2 Query: 281 QEDSADYCKGGYHPVKIGDLFLNR-YHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVKSA 457 +E++ DY GGYHPV IG+ F +R Y V RKLGWGHFSTVWL +D KR VALKVV+SA Sbjct: 56 EENAEDYHYGGYHPVYIGEEFHHRRYVVERKLGWGHFSTVWLAYDRAAKRRVALKVVRSA 115 Query: 458 PHFTETALDEIKILRAVETAIPPILRETKPVQLLNDFKITGVNGTHVCMVFEVLGHHLLK 637 H+ ET++DEI+IL+ + L + + LL+ F G NG HVCMVFEVLG +LL Sbjct: 116 EHYRETSIDEIRILQKIREGDEKHLGKKHIISLLDYFVHRGPNGAHVCMVFEVLGENLLS 175 Query: 638 LI 643 LI Sbjct: 176 LI 177 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 60.1 bits (139), Expect = 3e-10 Identities = 35/115 (30%), Positives = 58/115 (50%) Frame = +2 Query: 311 GYHPVKIGDLFLNRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVKSAPHFTETALDEI 490 G++ V F NRY V R LG G F V C+D R A+KV ++ P + E +L E+ Sbjct: 348 GHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIEL 407 Query: 491 KILRAVETAIPPILRETKPVQLLNDFKITGVNGTHVCMVFEVLGHHLLKLILRSN 655 ++L+ + + P E K +QL + F H+C+V ++ G + + +N Sbjct: 408 RVLQTIAHSDP--TNENKCIQLRDYFDYR----KHICIVTDLFGWSVFDFLKNNN 456 >SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 836 Score = 55.2 bits (127), Expect = 8e-09 Identities = 41/128 (32%), Positives = 62/128 (48%) Frame = +2 Query: 272 DEEQEDSADYCKGGYHPVKIGDLFLNRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVK 451 DE E D G Y ++ GD L RY + +G G F V C D + VA+KV+K Sbjct: 492 DECNEALFDDDNGDYKAIQ-GDHLLYRYEIIDTVGKGSFGQVLKCIDHKRGQVVAIKVIK 550 Query: 452 SAPHFTETALDEIKILRAVETAIPPILRETKPVQLLNDFKITGVNGTHVCMVFEVLGHHL 631 + F L E+ IL+ + A P + ++ L+ F G H+C+V E+LG +L Sbjct: 551 NRQKFHGQTLVEVGILKRLCEADP--ADKNNVIRYLSHFDFRG----HLCIVTELLGSNL 604 Query: 632 LKLILRSN 655 +I +N Sbjct: 605 FDVIRENN 612 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 54.4 bits (125), Expect = 1e-08 Identities = 41/115 (35%), Positives = 57/115 (49%) Frame = +2 Query: 311 GYHPVKIGDLFLNRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVKSAPHFTETALDEI 490 G + V +GD RY V LG G F V C D + VALK++++ F AL E Sbjct: 685 GDYKVVLGDHIAYRYEVVDFLGKGSFGQVLRCIDYETGKLVALKIIRNKKRFHMQALVET 744 Query: 491 KILRAVETAIPPILRETKPVQLLNDFKITGVNGTHVCMVFEVLGHHLLKLILRSN 655 KIL+ + P L E VQ + F H+C+ E+LG +L +LI +SN Sbjct: 745 KILQKIREWDP--LDEYCMVQYTDHFYFR----DHLCVATELLGKNLYELI-KSN 792 >SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizosaccharomyces pombe|chr 1|||Manual Length = 534 Score = 51.2 bits (117), Expect = 1e-07 Identities = 31/98 (31%), Positives = 52/98 (53%) Frame = +2 Query: 350 RYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVKSAPHFTETALDEIKILRAVETAIPPI 529 +Y + LG G F V C DL ++ VA+KV+K+ P F + E+ IL + P Sbjct: 129 KYLILDTLGHGTFGQVARCQDLKTQQIVAIKVIKNKPAFYNQCVMEVSILELLNNKYDP- 187 Query: 530 LRETKPVQLLNDFKITGVNGTHVCMVFEVLGHHLLKLI 643 + ++L + F ++ H+C+VFE+L +L +LI Sbjct: 188 EDKRHLIRLYDQF----MHKNHLCLVFELLSINLYELI 221 >SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosaccharomyces pombe|chr 3|||Manual Length = 477 Score = 45.2 bits (102), Expect = 8e-06 Identities = 36/122 (29%), Positives = 63/122 (51%) Frame = +2 Query: 278 EQEDSADYCKGGYHPVKIGDLFLNRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVKSA 457 + +D+ D +G Y V + +L +RY V LG G FSTV D + A+K++++ Sbjct: 135 DMQDNWDDIEGYYKVVLMEELD-SRYIVQSNLGKGMFSTVVSALDRNRNQTFAIKIIRNN 193 Query: 458 PHFTETALDEIKILRAVETAIPPILRETKPVQLLNDFKITGVNGTHVCMVFEVLGHHLLK 637 + L E+ IL ++ A RE K Q + ++ ++ H+CMVFE+L +L Sbjct: 194 EVMYKEGLKEVSILERLQAAD----REGK--QHIIHYERHFMHKNHLCMVFEMLSLNLRD 247 Query: 638 LI 643 ++ Sbjct: 248 IL 249 >SPBC32H8.10 |cdk9||cyclin-dependent protein kinase Cdk9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 33.9 bits (74), Expect = 0.021 Identities = 25/70 (35%), Positives = 34/70 (48%), Gaps = 5/70 (7%) Frame = +2 Query: 329 IGDLFLNRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVV---KSAPHFTETALDEIKIL 499 +G L YH+ KLG G F V+ D + ALK + F TA+ EIKIL Sbjct: 28 VGCSHLTDYHLMEKLGEGTFGEVYKSQRRKDGKVYALKRILMHTEKEGFPITAIREIKIL 87 Query: 500 RAV--ETAIP 523 +++ E IP Sbjct: 88 KSIKHENIIP 97 >SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosaccharomyces pombe|chr 1|||Manual Length = 988 Score = 31.1 bits (67), Expect = 0.15 Identities = 30/94 (31%), Positives = 43/94 (45%), Gaps = 1/94 (1%) Frame = +2 Query: 371 LGWGHFSTVWLCWDLVDKRFVALKVVKSAPHFTETALDEIKILRAVETAIPPILRETKPV 550 LG G+F V L V+K+F A+KV+K L+ +K E + + + K Sbjct: 670 LGKGNFGKVMLAEYKVNKKFYAIKVLKKEAILKNEELESLK----TEKHVFEVANKEKHP 725 Query: 551 QLLNDFKITGVNGTHVCMVFE-VLGHHLLKLILR 649 LLN F + T V V E +LG L+ I R Sbjct: 726 FLLNLF-ASFQTSTRVYFVMEYILGGDLMVHIQR 758 >SPAC9G1.09 |sid1||PAK-related kinase Sid1|Schizosaccharomyces pombe|chr 1|||Manual Length = 471 Score = 30.3 bits (65), Expect = 0.26 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = +2 Query: 347 NRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVKSAPHFTETALDEI 490 N Y + RKLG G F VW + V +A+K + ET +D+I Sbjct: 7 NSYTLLRKLGSGSFGVVWKARENVSGDIIAIKQID-----LETGIDDI 49 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 30.3 bits (65), Expect = 0.26 Identities = 23/71 (32%), Positives = 36/71 (50%), Gaps = 2/71 (2%) Frame = +2 Query: 323 VKIGDLFLNRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKV--VKSAPHFTETALDEIKI 496 +KIG N+Y + RK+G G F ++L + V+ VA+K+ +K+ H E I Sbjct: 6 IKIG----NKYRIGRKIGSGSFGQIYLGLNTVNGEQVAVKLEPLKARHHQLEYEFRVYNI 61 Query: 497 LRAVETAIPPI 529 L+ IP I Sbjct: 62 LKG-NIGIPTI 71 >SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 365 Score = 29.1 bits (62), Expect = 0.59 Identities = 12/33 (36%), Positives = 21/33 (63%) Frame = +2 Query: 347 NRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKV 445 N+Y + RK+G G F ++L ++V VA+K+ Sbjct: 9 NKYRIGRKIGSGSFGDIYLGTNVVSGEEVAIKL 41 >SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 496 Score = 29.1 bits (62), Expect = 0.59 Identities = 10/20 (50%), Positives = 16/20 (80%) Frame = +2 Query: 353 YHVTRKLGWGHFSTVWLCWD 412 YH+ R++G G F++V LC+D Sbjct: 10 YHIGREIGTGAFASVRLCYD 29 >SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 27.9 bits (59), Expect = 1.4 Identities = 12/34 (35%), Positives = 22/34 (64%) Frame = +2 Query: 350 RYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVK 451 RY + + LG G F+ V+L ++ +++ ALK +K Sbjct: 31 RYRIQKLLGEGGFAFVYLVQNVSNEKLYALKKIK 64 >SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = -1 Query: 125 NFVSFFLRLYGEYSSIYIRFRTH 57 NF +F L EY ++Y++F +H Sbjct: 756 NFTDWFASLGSEYENVYVKFISH 778 >SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 27.1 bits (57), Expect = 2.4 Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 2/87 (2%) Frame = -1 Query: 281 APRRRWCIAIFVFLNCFVGRTMRWF*TILVL-VTQTVSLANS-PVFFLAIFLFTNFVSFF 108 A +++ +++ + T+ WF I +T + N+ + F IF FVSF Sbjct: 33 AAKKKDLLSLISSKSLLSSETLGWFSVICAFSITGSGLEVNTMSIPFYLIFGIFAFVSFT 92 Query: 107 LRLYGEYSSIYIRFRTHLVVIISLSPI 27 + G YS + F+ V+I SLS + Sbjct: 93 TQYLGIYSFSFQPFQLLNVIIASLSMV 119 >SPAC2C4.14c |ppk11||PAK-related kinase Ppk11|Schizosaccharomyces pombe|chr 1|||Manual Length = 312 Score = 27.1 bits (57), Expect = 2.4 Identities = 17/53 (32%), Positives = 26/53 (49%), Gaps = 2/53 (3%) Frame = +2 Query: 347 NRYHVTRKLGWGHFSTVWLCWDLVDKRFVALKVV--KSAPHFTETALDEIKIL 499 ++Y + +G G F +V+ D+ + VALKVV + ET EI L Sbjct: 4 HQYRDLQLIGQGSFGSVFRAQDVESSKIVALKVVDLDATKDQIETLTQEINFL 56 >SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/22 (54%), Positives = 16/22 (72%) Frame = +2 Query: 338 LFLNRYHVTRKLGWGHFSTVWL 403 +F +RY V +KLG G F TV+L Sbjct: 16 IFEDRYLVKQKLGDGSFGTVYL 37 >SPAC24B11.11c |sid2||Sid2p-Mob1p kinase complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 607 Score = 25.8 bits (54), Expect = 5.5 Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = +2 Query: 353 YHVTRKLGWGHFSTVWLCWDLVDKRFVALKVV-KSAPHFTETALDEIK 493 + ++G G + +VWL K VALK++ KS H +DEI+ Sbjct: 208 FQTITQVGQGGYGSVWLARKRDTKEIVALKIMNKSVLH----KMDEIR 251 >SPAC688.06c |slx4||structure-specific endonuclease subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 25.8 bits (54), Expect = 5.5 Identities = 15/52 (28%), Positives = 25/52 (48%), Gaps = 5/52 (9%) Frame = +2 Query: 404 CWDLVDKRFVALKVVKSAPH-----FTETALDEIKILRAVETAIPPILRETK 544 C D +D R + K+ K PH T+T L+ I+ AI ++++ K Sbjct: 317 CLDAIDSRSQSKKLGKETPHDYLITSTKTVLEFDDIVTQTHRAISQVVKQAK 368 >SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|Schizosaccharomyces pombe|chr 3|||Manual Length = 288 Score = 25.4 bits (53), Expect = 7.3 Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 2/52 (3%) Frame = +2 Query: 353 YHVTRKLGWGHFSTVWLCWDLVDKRFVALKVVK-SAPHFT-ETALDEIKILR 502 Y KLG G ++ V+ + V VALKV++ A T TA+ EI +++ Sbjct: 3 YQRLEKLGEGTYAHVYKGQNRVTGEIVALKVIRIDADEGTPSTAIREISLMK 54 >SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosaccharomyces pombe|chr 1|||Manual Length = 650 Score = 25.4 bits (53), Expect = 7.3 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%) Frame = +2 Query: 353 YHVTRKLGWGHFSTVWLCWDLV-DKRFVALKVVK 451 Y+V RK+G G F +V+L K VA+K +K Sbjct: 36 YNVVRKVGDGTFGSVYLATTKTPSKEVVAIKSMK 69 >SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1402 Score = 25.0 bits (52), Expect = 9.6 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = -3 Query: 114 FFSSPVWRVLFYLHQI*NSFSSYYI 40 F+ S VW + +QI N+F + Y+ Sbjct: 1074 FYKSVVWTFTLFWYQIYNNFDANYL 1098 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 25.0 bits (52), Expect = 9.6 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +2 Query: 368 KLGWGHFSTVWLCWDLVDKRFVALKVVKSAPHFTETALDEIK 493 KLG G + +VWL +A+K++K + T L+E+K Sbjct: 167 KLGQGGYGSVWLAKKRNTHELLAMKMMKKS---TLQQLNEVK 205 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.0 bits (52), Expect = 9.6 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +2 Query: 275 EEQEDSADYCKGGYHPVKIGDLFLNRYHVTR 367 E D D C+G HP++ LFL Y +T+ Sbjct: 77 EIMNDLLDMCRGVQHPLR--GLFLRHYLLTQ 105 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,563,684 Number of Sequences: 5004 Number of extensions: 50256 Number of successful extensions: 168 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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