Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0443
         (655 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   145   4e-36
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   145   6e-36
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    92   7e-20
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    60   4e-10
SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch...    25   9.5  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    25   9.5  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  145 bits (352), Expect = 4e-36
 Identities = 61/82 (74%), Positives = 75/82 (91%)
 Frame = +2

Query: 8   FNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 187
           F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49  FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108

Query: 188 TIGKEIVDLVLDRIRKLADQCT 253
           T+GKE+VD V D+IR++AD C+
Sbjct: 109 TVGKELVDEVTDKIRRIADNCS 130



 Score =  123 bits (296), Expect = 3e-29
 Identities = 54/84 (64%), Positives = 67/84 (79%)
 Frame = +1

Query: 256 LQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTT 435
           LQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTT
Sbjct: 132 LQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTT 191

Query: 436 HTTLEHSDCAFMVDNEAIYDICRR 507
           H TL+ +DC FMVDNE+ YDICRR
Sbjct: 192 HATLDLADCTFMVDNESCYDICRR 215



 Score = 89.8 bits (213), Expect = 3e-19
 Identities = 41/48 (85%), Positives = 45/48 (93%)
 Frame = +3

Query: 510 LDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPR 653
           LDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPR
Sbjct: 217 LDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPR 264


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  145 bits (351), Expect = 6e-36
 Identities = 59/84 (70%), Positives = 75/84 (89%)
 Frame = +2

Query: 2   DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 181
           D F TFFSETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARG
Sbjct: 51  DGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARG 110

Query: 182 HYTIGKEIVDLVLDRIRKLADQCT 253
           HYT+GKE++D VL+RIR++AD C+
Sbjct: 111 HYTVGKEMIDSVLERIRRMADNCS 134



 Score =  128 bits (309), Expect = 7e-31
 Identities = 56/84 (66%), Positives = 68/84 (80%)
 Frame = +1

Query: 256 LQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTT 435
           LQGFL+FH            LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTT
Sbjct: 136 LQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTT 195

Query: 436 HTTLEHSDCAFMVDNEAIYDICRR 507
           H TL++SDC FMVDNEA YDICRR
Sbjct: 196 HATLDNSDCTFMVDNEACYDICRR 219



 Score = 89.8 bits (213), Expect = 3e-19
 Identities = 42/48 (87%), Positives = 44/48 (91%)
 Frame = +3

Query: 510 LDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPR 653
           LDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPR
Sbjct: 221 LDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPR 268


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 91.9 bits (218), Expect = 7e-20
 Identities = 39/83 (46%), Positives = 56/83 (67%)
 Frame = +2

Query: 2   DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 181
           +  N +F+E   GK+VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+G
Sbjct: 45  ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104

Query: 182 HYTIGKEIVDLVLDRIRKLADQC 250
           HYT G E+ D VLD +R+ A+ C
Sbjct: 105 HYTEGAELADAVLDVVRREAEAC 127



 Score = 63.3 bits (147), Expect = 3e-11
 Identities = 30/81 (37%), Positives = 45/81 (55%)
 Frame = +1

Query: 256 LQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTT 435
           LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  VVEPYN+ L+ 
Sbjct: 130 LQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSM 189

Query: 436 HTTLEHSDCAFMVDNEAIYDI 498
           H  +E+SD  F +DNEA+  I
Sbjct: 190 HQLVENSDETFCIDNEALSSI 210



 Score = 52.8 bits (121), Expect = 4e-08
 Identities = 21/48 (43%), Positives = 32/48 (66%)
 Frame = +3

Query: 510 LDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPR 653
           L I+ P+Y +LN L+  +++ +T S RF G LN DL +   N+VP+PR
Sbjct: 215 LKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPR 262


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 59.7 bits (138), Expect = 4e-10
 Identities = 29/83 (34%), Positives = 51/83 (61%), Gaps = 2/83 (2%)
 Frame = +2

Query: 2   DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYA 175
           D  + FF ++   +++PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A
Sbjct: 46  DRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWA 105

Query: 176 RGHYTIGKEIVDLVLDRIRKLAD 244
            G Y+  + I + ++D I + AD
Sbjct: 106 NG-YSHAERIFEDIMDMIDREAD 127



 Score = 48.4 bits (110), Expect = 9e-07
 Identities = 27/82 (32%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
 Frame = +1

Query: 256 LQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILT 432
           L+GF + H             L+ERL+  Y KK    ++++P  Q VS  VV+PYNS+L 
Sbjct: 132 LEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLA 191

Query: 433 THTTLEHSDCAFMVDNEAIYDI 498
                 ++D   ++DN A+  I
Sbjct: 192 LKRLTLNADSVVVLDNAALAHI 213



 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 17/48 (35%), Positives = 29/48 (60%)
 Frame = +3

Query: 510 LDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPR 653
           L  + PT+   N+L+  ++S+ T +LR+ G +N DL     +L+P PR
Sbjct: 218 LHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPR 265


>SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 525

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 9/33 (27%), Positives = 17/33 (51%)
 Frame = +3

Query: 228 SASSLTSVPSARIPDLPLLRWRYRLWVHFLIDG 326
           S +S+ S     + + P L WR+  W+  ++ G
Sbjct: 216 SVASVGSPMGTGVAENPKLTWRWLYWIDVIVGG 248


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/44 (27%), Positives = 24/44 (54%)
 Frame = -2

Query: 513 RDTAADVIDGFIVDHESTVRVLKGCVGGEDRVVGLDDGSGNLRR 382
           RDT  D++  +I+ +  T+  + GC+     + G+ D S  +R+
Sbjct: 831 RDTVLDLLGTYIMAYRETIPQIYGCI-----ISGISDPSTIVRK 869


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,563,397
Number of Sequences: 5004
Number of extensions: 49198
Number of successful extensions: 146
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)