SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0432
         (434 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    70   2e-11
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...    66   4e-10
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...    66   4e-10
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L...    66   4e-10
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...    66   4e-10
UniRef50_Q8DCJ7 Cluster: Methionine synthase; n=51; Bacteria|Rep...    58   1e-07
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp...    52   4e-06
UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8; Bacteria...    50   3e-05
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:...    47   2e-04
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ...    40   0.023
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba...    40   0.023
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ...    38   0.070
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la...    36   0.37 
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba...    35   0.65 
UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma j...    34   1.1  
UniRef50_A6RCM1 Cluster: Predicted protein; n=1; Ajellomyces cap...    33   3.5  
UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;...    32   4.6  
UniRef50_A0V9T2 Cluster: Uncharacterized protein UPF0065 precurs...    32   4.6  
UniRef50_Q6CKZ5 Cluster: Kluyveromyces lactis strain NRRL Y-1140...    32   4.6  
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ...    32   6.0  
UniRef50_Q9NKP4 Cluster: Putative uncharacterized protein; n=2; ...    32   6.0  
UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, wh...    32   6.0  
UniRef50_Q097Z5 Cluster: Putative uncharacterized protein; n=1; ...    31   8.0  
UniRef50_Q10F05 Cluster: Expressed protein; n=3; Oryza sativa (j...    31   8.0  
UniRef50_A4HH67 Cluster: DNA repair helicase, putative; n=3; Lei...    31   8.0  

>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 70.1 bits (164), Expect = 2e-11
 Identities = 35/54 (64%), Positives = 37/54 (68%), Gaps = 1/54 (1%)
 Frame = +1

Query: 166 HWPSFYNVVTGKTLALPNLIALQHIPLSPAA**RRGPHRS-PFPTVAQPEWRMA 324
           HWPSFYNVVTGKTLALPNLIALQHIPLSPA           P   +   +WRMA
Sbjct: 5   HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVISEEARTDRPSQQLRSLKWRMA 58


>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score = 65.7 bits (153), Expect = 4e-10
 Identities = 29/29 (100%), Positives = 29/29 (100%)
 Frame = +3

Query: 168 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 254
           LAVVLQRRDWENPGVTQLNRLAAHPPFAS
Sbjct: 68  LAVVLQRRDWENPGVTQLNRLAAHPPFAS 96



 Score = 52.8 bits (121), Expect = 3e-06
 Identities = 23/28 (82%), Positives = 24/28 (85%)
 Frame = +2

Query: 242 PFRQRRNSEEARTDRPSQQLRSLNGEWQ 325
           PF   RNSEEARTDRPSQQLR LNGEW+
Sbjct: 93  PFASWRNSEEARTDRPSQQLRXLNGEWR 120


>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score = 65.7 bits (153), Expect = 4e-10
 Identities = 29/29 (100%), Positives = 29/29 (100%)
 Frame = +3

Query: 168 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 254
           LAVVLQRRDWENPGVTQLNRLAAHPPFAS
Sbjct: 22  LAVVLQRRDWENPGVTQLNRLAAHPPFAS 50



 Score = 43.2 bits (97), Expect = 0.002
 Identities = 20/23 (86%), Positives = 20/23 (86%)
 Frame = +2

Query: 242 PFRQRRNSEEARTDRPSQQLRSL 310
           PF   RNSEEARTDRPSQQLRSL
Sbjct: 47  PFASWRNSEEARTDRPSQQLRSL 69


>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
           protein - Phage M13mp18
          Length = 102

 Score = 65.7 bits (153), Expect = 4e-10
 Identities = 29/29 (100%), Positives = 29/29 (100%)
 Frame = +3

Query: 168 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 254
           LAVVLQRRDWENPGVTQLNRLAAHPPFAS
Sbjct: 26  LAVVLQRRDWENPGVTQLNRLAAHPPFAS 54



 Score = 54.8 bits (126), Expect = 7e-07
 Identities = 24/28 (85%), Positives = 25/28 (89%)
 Frame = +2

Query: 242 PFRQRRNSEEARTDRPSQQLRSLNGEWQ 325
           PF   RNSEEARTDRPSQQLRSLNGEW+
Sbjct: 51  PFASWRNSEEARTDRPSQQLRSLNGEWR 78


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score = 65.7 bits (153), Expect = 4e-10
 Identities = 29/29 (100%), Positives = 29/29 (100%)
 Frame = +3

Query: 168 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 254
           LAVVLQRRDWENPGVTQLNRLAAHPPFAS
Sbjct: 8   LAVVLQRRDWENPGVTQLNRLAAHPPFAS 36



 Score = 54.8 bits (126), Expect = 7e-07
 Identities = 24/28 (85%), Positives = 25/28 (89%)
 Frame = +2

Query: 242 PFRQRRNSEEARTDRPSQQLRSLNGEWQ 325
           PF   RNSEEARTDRPSQQLRSLNGEW+
Sbjct: 33  PFASWRNSEEARTDRPSQQLRSLNGEWR 60


>UniRef50_Q8DCJ7 Cluster: Methionine synthase; n=51; Bacteria|Rep:
           Methionine synthase - Vibrio vulnificus
          Length = 1226

 Score = 57.6 bits (133), Expect = 1e-07
 Identities = 26/38 (68%), Positives = 28/38 (73%)
 Frame = -1

Query: 116 RTRKEYETVRIQHGRKKPRTPPVTLEAARDNDFAFDWQ 3
           R + +YE VR QH RKKPRT PVTLEAAR N  A DWQ
Sbjct: 883 RLQADYELVRDQHNRKKPRTKPVTLEAARANKVAIDWQ 920


>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
           spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
           spumigena CCY 9414
          Length = 72

 Score = 52.4 bits (120), Expect = 4e-06
 Identities = 22/25 (88%), Positives = 25/25 (100%)
 Frame = +2

Query: 257 RNSEEARTDRPSQQLRSLNGEWQIV 331
           RNSEEARTDRPSQQLRSLNGEW+++
Sbjct: 48  RNSEEARTDRPSQQLRSLNGEWRLM 72


>UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8;
           Bacteria|Rep: 50S ribosomal protein L5 - Moritella sp.
           PE36
          Length = 45

 Score = 49.6 bits (113), Expect = 3e-05
 Identities = 23/26 (88%), Positives = 24/26 (92%)
 Frame = -2

Query: 331 YNLPFAIQAAQLLGRAIGAGLFAITP 254
           +  PFAIQAAQLLGRAIGAGLFAITP
Sbjct: 8   HQAPFAIQAAQLLGRAIGAGLFAITP 33


>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
           Beta-galactosidase - Yersinia pseudotuberculosis
          Length = 1066

 Score = 46.8 bits (106), Expect = 2e-04
 Identities = 19/29 (65%), Positives = 22/29 (75%)
 Frame = +3

Query: 168 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 254
           L  +L RRDWENP +TQ +RL AHPPF S
Sbjct: 15  LPQILSRRDWENPQITQYHRLEAHPPFHS 43



 Score = 33.5 bits (73), Expect = 2.0
 Identities = 14/27 (51%), Positives = 18/27 (66%)
 Frame = +2

Query: 242 PFRQRRNSEEARTDRPSQQLRSLNGEW 322
           PF   R+ E A+ DRPS Q ++LNG W
Sbjct: 40  PFHSWRDVESAQKDRPSPQQQTLNGLW 66


>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
           Enterobacter sakazakii ATCC BAA-894|Rep: Putative
           uncharacterized protein - Enterobacter sakazakii ATCC
           BAA-894
          Length = 1043

 Score = 39.9 bits (89), Expect = 0.023
 Identities = 14/26 (53%), Positives = 19/26 (73%)
 Frame = +3

Query: 168 LAVVLQRRDWENPGVTQLNRLAAHPP 245
           LA +L R DW+NP +T +NRL +H P
Sbjct: 18  LATILARNDWQNPAITSVNRLPSHTP 43


>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
           Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
           pneumoniae
          Length = 1034

 Score = 39.9 bits (89), Expect = 0.023
 Identities = 17/26 (65%), Positives = 18/26 (69%)
 Frame = +3

Query: 177 VLQRRDWENPGVTQLNRLAAHPPFAS 254
           VL R DW N  +T LNRL AHP FAS
Sbjct: 17  VLAREDWHNQTITHLNRLPAHPVFAS 42


>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
           Plasmodium (Vinckeia)|Rep: Putative uncharacterized
           protein - Plasmodium berghei
          Length = 275

 Score = 38.3 bits (85), Expect = 0.070
 Identities = 17/18 (94%), Positives = 17/18 (94%)
 Frame = +1

Query: 109 RVRGGARYPIRPIVSRIT 162
           R RGGARYPIRPIVSRIT
Sbjct: 258 RPRGGARYPIRPIVSRIT 275


>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
           lactis|Rep: Beta-galactosidase - Lactococcus lactis
           subsp. lactis (Streptococcus lactis)
          Length = 998

 Score = 35.9 bits (79), Expect = 0.37
 Identities = 14/23 (60%), Positives = 17/23 (73%)
 Frame = +3

Query: 177 VLQRRDWENPGVTQLNRLAAHPP 245
           VL+R+DWENP V+  NRL  H P
Sbjct: 9   VLERKDWENPVVSNWNRLPMHTP 31


>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
           Gammaproteobacteria|Rep: Beta-galactosidase -
           Pseudoalteromonas haloplanktis (Alteromonas
           haloplanktis)
          Length = 1039

 Score = 35.1 bits (77), Expect = 0.65
 Identities = 12/23 (52%), Positives = 17/23 (73%)
 Frame = +3

Query: 177 VLQRRDWENPGVTQLNRLAAHPP 245
           ++ RRDWENP   Q+N++ AH P
Sbjct: 7   IINRRDWENPITVQVNQVKAHSP 29


>UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma
           japonicum|Rep: SJCHGC09076 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 109

 Score = 34.3 bits (75), Expect = 1.1
 Identities = 17/37 (45%), Positives = 21/37 (56%)
 Frame = +3

Query: 171 AVVLQRRDWENPGVTQLNRLAAHPPFASGVIAKRPAP 281
           A  L+RR+ +NPG  QLN L A P F  G   K+  P
Sbjct: 57  AAFLKRREGKNPGCPQLNPLEALPLFPGGEKTKKAPP 93


>UniRef50_A6RCM1 Cluster: Predicted protein; n=1; Ajellomyces
           capsulatus NAm1|Rep: Predicted protein - Ajellomyces
           capsulatus NAm1
          Length = 542

 Score = 32.7 bits (71), Expect = 3.5
 Identities = 13/20 (65%), Positives = 16/20 (80%)
 Frame = -3

Query: 132 VPGPPSYPQGVRNRTYSARA 73
           VP PPS+PQGV++ T  ARA
Sbjct: 303 VPAPPSHPQGVQSETNQARA 322


>UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;
           n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
           Danio rerio
          Length = 195

 Score = 32.3 bits (70), Expect = 4.6
 Identities = 13/13 (100%), Positives = 13/13 (100%)
 Frame = +3

Query: 168 LAVVLQRRDWENP 206
           LAVVLQRRDWENP
Sbjct: 179 LAVVLQRRDWENP 191


>UniRef50_A0V9T2 Cluster: Uncharacterized protein UPF0065 precursor;
           n=2; Comamonadaceae|Rep: Uncharacterized protein UPF0065
           precursor - Delftia acidovorans SPH-1
          Length = 330

 Score = 32.3 bits (70), Expect = 4.6
 Identities = 14/40 (35%), Positives = 22/40 (55%)
 Frame = -2

Query: 325 LPFAIQAAQLLGRAIGAGLFAITPLAKGGCAARRLSWVTP 206
           +PF +     L  A+ A  FA+   A+GG  A+ L+W+ P
Sbjct: 1   MPFHLPRLPRLALALAATTFALAAHAQGGYPAKPLTWIVP 40


>UniRef50_Q6CKZ5 Cluster: Kluyveromyces lactis strain NRRL Y-1140
            chromosome F of strain NRRL Y- 1140 of Kluyveromyces
            lactis; n=1; Kluyveromyces lactis|Rep: Kluyveromyces
            lactis strain NRRL Y-1140 chromosome F of strain NRRL Y-
            1140 of Kluyveromyces lactis - Kluyveromyces lactis
            (Yeast) (Candida sphaerica)
          Length = 1322

 Score = 32.3 bits (70), Expect = 4.6
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = -3

Query: 369  QKFNANFNKILTLTICHSPFRLRNCWEGRSVR 274
            +K++ N N++L + I H  F LR+C+E   +R
Sbjct: 1137 KKYDENLNELLKIVIIHEKFILRHCFESIELR 1168


>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
           uncultured bacterium|Rep: Non-ribosomal peptide
           synthetase - uncultured bacterium
          Length = 338

 Score = 31.9 bits (69), Expect = 6.0
 Identities = 17/34 (50%), Positives = 17/34 (50%)
 Frame = -2

Query: 217 WVTPGFSQSRRCKTTASEL*YDSL*GELGTGPPL 116
           W   GF     C        YDSL GELGTGPPL
Sbjct: 260 WSKTGFRPF--CLEAGRRAYYDSLYGELGTGPPL 291


>UniRef50_Q9NKP4 Cluster: Putative uncharacterized protein; n=2;
            Leishmania|Rep: Putative uncharacterized protein -
            Leishmania major
          Length = 2382

 Score = 31.9 bits (69), Expect = 6.0
 Identities = 15/39 (38%), Positives = 20/39 (51%)
 Frame = -2

Query: 322  PFAIQAAQLLGRAIGAGLFAITPLAKGGCAARRLSWVTP 206
            P+A+ AA   G   G G    + +A  G AA R +WV P
Sbjct: 1063 PWAVAAAAATGSRTGTGTETSSSVASDGAAAPRRAWVPP 1101


>UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, whole
           genome shotgun sequence; n=1; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_33,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 1173

 Score = 31.9 bits (69), Expect = 6.0
 Identities = 15/42 (35%), Positives = 24/42 (57%)
 Frame = -3

Query: 432 SFDL*GILPISAYWLKNELI*QKFNANFNKILTLTICHSPFR 307
           +F L G L    +WLKN+    KF++ F ++L L +  + FR
Sbjct: 667 NFSLQGALSYIDFWLKNQHFDDKFSSTFTQLLLLALGVTVFR 708


>UniRef50_Q097Z5 Cluster: Putative uncharacterized protein; n=1;
           Stigmatella aurantiaca DW4/3-1|Rep: Putative
           uncharacterized protein - Stigmatella aurantiaca DW4/3-1
          Length = 516

 Score = 31.5 bits (68), Expect = 8.0
 Identities = 18/49 (36%), Positives = 25/49 (51%)
 Frame = -2

Query: 316 AIQAAQLLGRAIGAGLFAITPLAKGGCAARRLSWVTPGFSQSRRCKTTA 170
           A  ++ L GRA GAG+  +     GG A+R   W + G   SRR +  A
Sbjct: 138 AATSSSLTGRASGAGMTRVR--VPGGRASRESRWASTGAPPSRRYRYNA 184


>UniRef50_Q10F05 Cluster: Expressed protein; n=3; Oryza sativa
           (japonica cultivar-group)|Rep: Expressed protein - Oryza
           sativa subsp. japonica (Rice)
          Length = 162

 Score = 31.5 bits (68), Expect = 8.0
 Identities = 19/51 (37%), Positives = 25/51 (49%)
 Frame = -2

Query: 187 RCKTTASEL*YDSL*GELGTGPPLVPARSTKPYVFSTGVRNRAHHRSRWKR 35
           R +   S L   +L  +LG G  LVPA     Y+ STG+  R    S W+R
Sbjct: 3   RARRLGSRLANLALGTDLGEGRILVPAYPNTLYIRSTGIPRRQLEGSGWRR 53


>UniRef50_A4HH67 Cluster: DNA repair helicase, putative; n=3;
           Leishmania|Rep: DNA repair helicase, putative -
           Leishmania braziliensis
          Length = 1108

 Score = 31.5 bits (68), Expect = 8.0
 Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = +1

Query: 100 YSLRVRGGARYPIRP-IVSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSP 252
           Y  +VR G    ++  + S+  I    +Y+ V  +TL +PNL   +H+ L P
Sbjct: 432 YKCQVRDGKLREVKERLFSKFGIRADCYYDYVQDRTLYVPNLTLARHVRLRP 483


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 466,377,473
Number of Sequences: 1657284
Number of extensions: 9747939
Number of successful extensions: 26222
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 25593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26218
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 21496989549
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -