BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0425 (443 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 130 7e-32 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 69 3e-13 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 66 2e-12 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 66 2e-12 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 48 6e-07 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 46 3e-06 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 38 0.001 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.14 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 26 2.3 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 2.3 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.0 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 3.0 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 25 6.9 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 25 6.9 SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 25 6.9 SPBC2A9.05c |||DUF846 family protein|Schizosaccharomyces pombe|c... 24 9.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 130 bits (315), Expect = 7e-32 Identities = 57/63 (90%), Positives = 59/63 (93%) Frame = +3 Query: 255 SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPDEHPVLLTEAALNPKANREKM 434 SKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAP+EHP LLTEA LNPK+NREKM Sbjct: 60 SKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKM 119 Query: 435 THI 443 T I Sbjct: 120 TQI 122 Score = 120 bits (289), Expect = 1e-28 Identities = 52/60 (86%), Positives = 58/60 (96%) Frame = +1 Query: 82 DEEVAALVVDNGSGMCKAGFSGDDAPRAVFPSIVGRPRHQGVMVGMGHKDSYVGDEAQAK 261 +EE+AALV+DNGSGMCKAGF+GDDAPRAVFPSIVGRPRH G+MVGMG KDSYVGDEAQ+K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 68.9 bits (161), Expect = 3e-13 Identities = 26/58 (44%), Positives = 44/58 (75%) Frame = +3 Query: 261 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPDEHPVLLTEAALNPKANREKM 434 R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE +NP ANREKM Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKM 119 Score = 54.4 bits (125), Expect = 7e-09 Identities = 29/59 (49%), Positives = 38/59 (64%), Gaps = 2/59 (3%) Frame = +1 Query: 88 EVAALVVDNGSGMCKAGFSGDDAPRAVFPSIVGRP--RHQGVMVGMGHKDSYVGDEAQA 258 E A +V+DNG+G K G++ D+ PR FPSIVGRP R + + KD VGDEA+A Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 66.1 bits (154), Expect = 2e-12 Identities = 27/57 (47%), Positives = 34/57 (59%) Frame = +3 Query: 273 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPDEHPVLLTEAALNPKANREKMTHI 443 +L YPI HG + NWD ME+ W + + LR P++H LLTE LNP NRE I Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEI 145 Score = 41.1 bits (92), Expect = 7e-05 Identities = 19/52 (36%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 100 LVVDNGSGMCKAGFSGDDAPRAVFPSIVGRPRHQGVMVG--MGHKDSYVGDE 249 +++DNG+G K G++G+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 66.1 bits (154), Expect = 2e-12 Identities = 28/52 (53%), Positives = 37/52 (71%) Frame = +1 Query: 100 LVVDNGSGMCKAGFSGDDAPRAVFPSIVGRPRHQGVMVGMGHKDSYVGDEAQ 255 + +DNGSG KAGF+GDD P+ +FP+ VGR +H+ VM KD +VG EAQ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQ 63 Score = 64.9 bits (151), Expect = 5e-12 Identities = 28/59 (47%), Positives = 41/59 (69%), Gaps = 1/59 (1%) Frame = +3 Query: 261 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPDEHPVLLTEAALNPKANREKM 434 RG+L ++ PIE GI+ NW DME+IW + + + +L P+EHP+LLTE L N+EK+ Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKI 124 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 48.0 bits (109), Expect = 6e-07 Identities = 21/54 (38%), Positives = 30/54 (55%) Frame = +3 Query: 270 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPDEHPVLLTEAALNPKANREK 431 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVK 116 Score = 41.5 bits (93), Expect = 6e-05 Identities = 17/34 (50%), Positives = 24/34 (70%) Frame = +1 Query: 85 EEVAALVVDNGSGMCKAGFSGDDAPRAVFPSIVG 186 +EV+A+V+D GS + GFSG+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 45.6 bits (103), Expect = 3e-06 Identities = 20/53 (37%), Positives = 31/53 (58%) Frame = +3 Query: 285 PIEHGIVTNWDDMEKIWHHTFYNELRVAPDEHPVLLTEAALNPKANREKMTHI 443 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT + Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTEL 137 Score = 35.9 bits (79), Expect = 0.003 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +1 Query: 100 LVVDNGSGMCKAGFSGDDAPRAVFPSIVGRPR 195 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 37.5 bits (83), Expect = 0.001 Identities = 19/57 (33%), Positives = 33/57 (57%) Frame = +1 Query: 85 EEVAALVVDNGSGMCKAGFSGDDAPRAVFPSIVGRPRHQGVMVGMGHKDSYVGDEAQ 255 EE+ +LV+D GS + G++G+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 Score = 36.7 bits (81), Expect = 0.002 Identities = 13/47 (27%), Positives = 29/47 (61%) Frame = +3 Query: 294 HGIVTNWDDMEKIWHHTFYNELRVAPDEHPVLLTEAALNPKANREKM 434 +GI+ +W+ W +L+V P E+ +++TE + NP++ R+++ Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQI 120 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 30.3 bits (65), Expect = 0.14 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -1 Query: 242 PT*ESLCPI-PTITP*WRGLPTIEGNTARGASSPEKPALHIPEPLSTTNAATSSS 81 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 26.2 bits (55), Expect = 2.3 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 2/35 (5%) Frame = +3 Query: 345 FYNELRVAPDEHPVLLTE--AALNPKANREKMTHI 443 +Y L ++HP+LLT+ AAL P+ + ++ I Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRI 1433 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 26.2 bits (55), Expect = 2.3 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +1 Query: 145 GDDAPRAVFPSIVGRPRHQGVMVGMGHKDSYVGDE 249 G+ PRA F ++ P H G+++ M KD G+E Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 25.8 bits (54), Expect = 3.0 Identities = 13/31 (41%), Positives = 15/31 (48%) Frame = -1 Query: 221 PIPTITP*WRGLPTIEGNTARGASSPEKPAL 129 P+PT P LPT NT + P PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 25.8 bits (54), Expect = 3.0 Identities = 7/21 (33%), Positives = 13/21 (61%) Frame = +3 Query: 285 PIEHGIVTNWDDMEKIWHHTF 347 PI+ G V +W+ ++ W H + Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 24.6 bits (51), Expect = 6.9 Identities = 17/66 (25%), Positives = 28/66 (42%) Frame = -1 Query: 317 IPVSYDSVFDGVFEGQDTSFA*ASSPT*ESLCPIPTITP*WRGLPTIEGNTARGASSPEK 138 +P +V +TSFA ++ SL PT + P +E + +S+P Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 137 PALHIP 120 P L+ P Sbjct: 123 PQLNSP 128 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 24.6 bits (51), Expect = 6.9 Identities = 11/41 (26%), Positives = 26/41 (63%), Gaps = 3/41 (7%) Frame = +3 Query: 300 IVTNWDDME-KIWH--HTFYNELRVAPDEHPVLLTEAALNP 413 ++ + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 271 VIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 >SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 24.6 bits (51), Expect = 6.9 Identities = 15/43 (34%), Positives = 18/43 (41%) Frame = -1 Query: 383 GVFIGGDTQLIVEGMMPNLLHVIPVSYDSVFDGVFEGQDTSFA 255 GVF GD ++ PN VI D D F D SF+ Sbjct: 265 GVFTTGDICKVLRKEFPNKSDVIAEPVDITVDPSFFKLDNSFS 307 >SPBC2A9.05c |||DUF846 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 219 Score = 24.2 bits (50), Expect = 9.1 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = -3 Query: 132 LAHTGAIVYYQRGNFFVAHLELVF 61 L TGAIV Y G FF + L+F Sbjct: 56 LFRTGAIVAYILGMFFTSSFMLLF 79 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,796,744 Number of Sequences: 5004 Number of extensions: 36164 Number of successful extensions: 113 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 98 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 110 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 162176800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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