Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0417
(345 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P12694 Cluster: 2-oxoisovalerate dehydrogenase subunit ... 54 5e-07
UniRef50_Q9VHB8 Cluster: CG8199-PA; n=2; Eukaryota|Rep: CG8199-P... 54 9e-07
UniRef50_Q295J7 Cluster: GA20891-PA; n=7; Coelomata|Rep: GA20891... 53 2e-06
UniRef50_Q4A1S8 Cluster: Putative uncharacterized protein; n=4; ... 50 8e-06
UniRef50_A7RZV6 Cluster: Predicted protein; n=6; Eumetazoa|Rep: ... 50 8e-06
UniRef50_Q9LPL5 Cluster: Branched-chain alpha keto-acid dehydrog... 44 7e-04
UniRef50_A0DAM1 Cluster: Chromosome undetermined scaffold_43, wh... 41 0.005
UniRef50_Q4P2J0 Cluster: Putative uncharacterized protein; n=1; ... 40 0.009
UniRef50_Q4QC52 Cluster: 2-oxoisovalerate dehydrogenase alpha su... 39 0.026
UniRef50_Q5KGR5 Cluster: Branched-chain alpha-keto acid dehydrog... 38 0.035
UniRef50_Q4Y3F8 Cluster: Branched-chain alpha keto-acid dehydrog... 38 0.060
UniRef50_Q4N1L6 Cluster: Branched-chain alpha keto-acid dehydrog... 36 0.14
UniRef50_Q4DB65 Cluster: 2-oxoisovalerate dehydrogenase alpha su... 36 0.14
UniRef50_A6RV39 Cluster: Putative uncharacterized protein; n=1; ... 36 0.14
UniRef50_Q1KSF2 Cluster: Mitochondrial branched-chain alpha-keto... 36 0.18
UniRef50_Q6B8N3 Cluster: Conserved hypothetical plastid protein;... 32 3.0
UniRef50_Q1G3P8 Cluster: Putative uncharacterized protein; n=1; ... 32 3.0
UniRef50_Q22X19 Cluster: Cation channel family protein; n=2; Alv... 31 5.3
UniRef50_A3LW72 Cluster: Predicted protein; n=1; Pichia stipitis... 31 5.3
UniRef50_Q5FBD1 Cluster: Serine/threonine protein kinase ARAF; n... 31 6.9
UniRef50_A0C038 Cluster: Chromosome undetermined scaffold_14, wh... 31 6.9
UniRef50_Q6BIN7 Cluster: Similar to tr|Q96VL6 Candida albicans P... 31 6.9
UniRef50_A5E9P6 Cluster: Putative branched-chain amino acid amin... 30 9.2
>UniRef50_P12694 Cluster: 2-oxoisovalerate dehydrogenase subunit
alpha, mitochondrial precursor; n=29; Euteleostomi|Rep:
2-oxoisovalerate dehydrogenase subunit alpha,
mitochondrial precursor - Homo sapiens (Human)
Length = 445
Score = 54.4 bits (125), Expect = 5e-07
Identities = 23/56 (41%), Positives = 36/56 (64%)
Frame = +3
Query: 93 PEFPGARAPYLSEMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNLDKATLINMSK 260
P+FPGA A ++ +++F IPIY VMD GQII+ +E+P+L K ++ + K
Sbjct: 52 PQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYK 107
Score = 51.2 bits (117), Expect = 5e-06
Identities = 22/29 (75%), Positives = 26/29 (89%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTNY 343
K+M L+ MD+I+YESQRQGRISFYMTNY
Sbjct: 107 KSMTLLNTMDRILYESQRQGRISFYMTNY 135
>UniRef50_Q9VHB8 Cluster: CG8199-PA; n=2; Eukaryota|Rep: CG8199-PA -
Drosophila melanogaster (Fruit fly)
Length = 439
Score = 53.6 bits (123), Expect = 9e-07
Identities = 22/52 (42%), Positives = 32/52 (61%)
Frame = +3
Query: 99 FPGARAPYLSEMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNLDKATLINM 254
FPGA+AP++S++ Y PIPIY VMD +G I D ++P L + + M
Sbjct: 46 FPGAKAPFVSKLNLIQPEDYAPIPIYRVMDQDGYIADETQDPQLGREVVEKM 97
Score = 50.4 bits (115), Expect = 8e-06
Identities = 22/29 (75%), Positives = 26/29 (89%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTNY 343
+ MV L+ MDKI+YESQRQGRISFYMTN+
Sbjct: 99 RDMVLLNTMDKILYESQRQGRISFYMTNF 127
>UniRef50_Q295J7 Cluster: GA20891-PA; n=7; Coelomata|Rep: GA20891-PA
- Drosophila pseudoobscura (Fruit fly)
Length = 439
Score = 52.8 bits (121), Expect = 2e-06
Identities = 23/52 (44%), Positives = 33/52 (63%)
Frame = +3
Query: 99 FPGARAPYLSEMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNLDKATLINM 254
FPGA+AP++S++K Y PIPIY VMD +G I D ++P L + + M
Sbjct: 46 FPGAKAPFVSKLKLNMPEDYAPIPIYRVMDRDGFIADETQDPQLGREVVEKM 97
Score = 49.2 bits (112), Expect = 2e-05
Identities = 21/29 (72%), Positives = 26/29 (89%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTNY 343
+ M+ L+ MDKI+YESQRQGRISFYMTN+
Sbjct: 99 RDMLLLNTMDKILYESQRQGRISFYMTNF 127
>UniRef50_Q4A1S8 Cluster: Putative uncharacterized protein; n=4;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 432
Score = 50.4 bits (115), Expect = 8e-06
Identities = 21/29 (72%), Positives = 27/29 (93%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTNY 343
KTM QL+ MD+I+Y+SQRQGRISFYMT++
Sbjct: 91 KTMTQLNIMDRILYDSQRQGRISFYMTSF 119
Score = 36.7 bits (81), Expect = 0.11
Identities = 15/50 (30%), Positives = 28/50 (56%)
Frame = +3
Query: 111 RAPYLSEMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNLDKATLINMSK 260
+A + +++ N +PIY V + G +ID +++PN D+ T + M K
Sbjct: 42 KAAFTEKLEIVNADDTPALPIYRVTNAVGDVIDKSQDPNFDEQTSLKMYK 91
>UniRef50_A7RZV6 Cluster: Predicted protein; n=6; Eumetazoa|Rep:
Predicted protein - Nematostella vectensis
Length = 444
Score = 50.4 bits (115), Expect = 8e-06
Identities = 22/29 (75%), Positives = 25/29 (86%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTNY 343
K M L+ MD+I+YESQRQGRISFYMTNY
Sbjct: 105 KKMTLLNTMDRILYESQRQGRISFYMTNY 133
Score = 38.7 bits (86), Expect = 0.026
Identities = 19/58 (32%), Positives = 34/58 (58%), Gaps = 2/58 (3%)
Frame = +3
Query: 93 PEFPGA-RAPYLSEMKFFNETSYEPI-PIYPVMDNNGQIIDLNEEPNLDKATLINMSK 260
P +PGA + +++F + + I P+Y VMD G+II + +P L + T+++M K
Sbjct: 48 PRYPGAMNCSFTEKLEFVDPMDPQGIIPVYRVMDRQGKIIIDSHDPKLPEGTIVDMYK 105
>UniRef50_Q9LPL5 Cluster: Branched-chain alpha keto-acid
dehydrogenase E1-alpha subunit; n=13; Magnoliophyta|Rep:
Branched-chain alpha keto-acid dehydrogenase E1-alpha
subunit - Arabidopsis thaliana (Mouse-ear cress)
Length = 472
Score = 44.0 bits (99), Expect = 7e-04
Identities = 19/37 (51%), Positives = 25/37 (67%)
Frame = +3
Query: 96 EFPGARAPYLSEMKFFNETSYEPIPIYPVMDNNGQII 206
+FPG + Y SEMKF E+S IP Y V+D +G+II
Sbjct: 81 DFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRII 117
Score = 39.1 bits (87), Expect = 0.020
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +2
Query: 263 MVQLSHMDKIIYESQRQGRISFYMTN 340
M L MD I YE+QRQGRISFY+T+
Sbjct: 137 MATLQVMDHIFYEAQRQGRISFYLTS 162
>UniRef50_A0DAM1 Cluster: Chromosome undetermined scaffold_43, whole
genome shotgun sequence; n=3; Oligohymenophorea|Rep:
Chromosome undetermined scaffold_43, whole genome
shotgun sequence - Paramecium tetraurelia
Length = 406
Score = 41.1 bits (92), Expect = 0.005
Identities = 15/27 (55%), Positives = 22/27 (81%)
Frame = +2
Query: 263 MVQLSHMDKIIYESQRQGRISFYMTNY 343
M+ + MD ++Y SQRQG+ISFYMT++
Sbjct: 66 MISIEEMDNLLYMSQRQGKISFYMTSF 92
>UniRef50_Q4P2J0 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 786
Score = 40.3 bits (90), Expect = 0.009
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTNY 343
+TM+ L +D I+Y +QRQGRISF MT+Y
Sbjct: 430 RTMLLLPQIDVILYNAQRQGRISFMMTSY 458
>UniRef50_Q4QC52 Cluster: 2-oxoisovalerate dehydrogenase alpha
subunit, putative; n=3; Leishmania|Rep: 2-oxoisovalerate
dehydrogenase alpha subunit, putative - Leishmania major
Length = 479
Score = 38.7 bits (86), Expect = 0.026
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +2
Query: 254 VKTMVQLSHMDKIIYESQRQGRISFYMTNY 343
+ M+ + MDKI+ E+QRQGRISFYMT +
Sbjct: 125 MSAMLTHNTMDKIMLEAQRQGRISFYMTMF 154
>UniRef50_Q5KGR5 Cluster: Branched-chain alpha-keto acid
dehydrogenase E1-alpha subunit, putative; n=2;
Filobasidiella neoformans|Rep: Branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit, putative -
Cryptococcus neoformans (Filobasidiella neoformans)
Length = 504
Score = 38.3 bits (85), Expect = 0.035
Identities = 15/26 (57%), Positives = 21/26 (80%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYM 334
+TM + +D ++Y+SQRQGRISFYM
Sbjct: 140 RTMTLIPIVDNVLYQSQRQGRISFYM 165
>UniRef50_Q4Y3F8 Cluster: Branched-chain alpha keto-acid
dehydrogenase, putative; n=7; Plasmodium|Rep:
Branched-chain alpha keto-acid dehydrogenase, putative -
Plasmodium chabaudi
Length = 432
Score = 37.5 bits (83), Expect = 0.060
Identities = 17/28 (60%), Positives = 20/28 (71%)
Frame = +2
Query: 257 KTMVQLSHMDKIIYESQRQGRISFYMTN 340
K MV+ S D+I Y QRQGRISFY+ N
Sbjct: 91 KQMVEFSIWDEIFYGIQRQGRISFYIVN 118
Score = 33.9 bits (74), Expect = 0.75
Identities = 16/73 (21%), Positives = 41/73 (56%), Gaps = 2/73 (2%)
Frame = +3
Query: 120 YLSEMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNLDKATLINMSKPWYN*AIWIKLY- 296
+ +++K NE + +PI+ ++D NG ++D + P D ++N+ K +IW +++
Sbjct: 48 FSTDLKTVNEVA--KLPIFRILDTNGNLLDGHTAP-FDDEEVLNLYKQMVEFSIWDEIFY 104
Query: 297 -MNHKDKAAFHFI 332
+ + + +F+ +
Sbjct: 105 GIQRQGRISFYIV 117
>UniRef50_Q4N1L6 Cluster: Branched-chain alpha keto-acid
dehydrogenase, putative; n=3; Piroplasmida|Rep:
Branched-chain alpha keto-acid dehydrogenase, putative -
Theileria parva
Length = 464
Score = 36.3 bits (80), Expect = 0.14
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +2
Query: 251 HVKTMVQLSHMDKIIYESQRQGRISFYMTN 340
+++ MV+L+ D + Y QRQGRISFY+ N
Sbjct: 109 YLQIMVKLNVWDNLFYNIQRQGRISFYIQN 138
>UniRef50_Q4DB65 Cluster: 2-oxoisovalerate dehydrogenase alpha
subunit, putative; n=3; Trypanosoma|Rep:
2-oxoisovalerate dehydrogenase alpha subunit, putative -
Trypanosoma cruzi
Length = 431
Score = 36.3 bits (80), Expect = 0.14
Identities = 15/29 (51%), Positives = 23/29 (79%)
Frame = +2
Query: 254 VKTMVQLSHMDKIIYESQRQGRISFYMTN 340
++ M++ +D I+ E+QRQGRISFYMT+
Sbjct: 90 MEAMLRQQSIDLILMEAQRQGRISFYMTS 118
Score = 35.9 bits (79), Expect = 0.18
Identities = 15/46 (32%), Positives = 29/46 (63%)
Frame = +3
Query: 117 PYLSEMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNLDKATLINM 254
P + E+ F ++ Y P++ V+D +G++++ +EP + K TLI M
Sbjct: 45 PTVDELTF-HQRKYPSTPMFHVLDLDGRVVNPKQEPEVPKETLIKM 89
>UniRef50_A6RV39 Cluster: Putative uncharacterized protein; n=1;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 211
Score = 36.3 bits (80), Expect = 0.14
Identities = 15/24 (62%), Positives = 21/24 (87%)
Frame = +2
Query: 263 MVQLSHMDKIIYESQRQGRISFYM 334
M+++S MD I+ E+QRQGR+SFYM
Sbjct: 108 MIKVSIMDVIMVEAQRQGRLSFYM 131
Score = 35.1 bits (77), Expect = 0.32
Identities = 28/81 (34%), Positives = 41/81 (50%), Gaps = 5/81 (6%)
Frame = +3
Query: 33 LGFCRPVRGPKRHI-MAVVR*PEFPGA-RAPYLSEMKFFNETSYEPIPIYPVMDNNGQII 206
LG C GP + A VR FPGA + + S + F + ++Y IP Y M +G+I+
Sbjct: 33 LGRCHSTVGPSQKPGSAGVR---FPGAVDSKFTSSLSFEHPSTYNAIPTYRTMSPDGEIL 89
Query: 207 DLNEEPNLDKATL---INMSK 260
D + D+A L +NM K
Sbjct: 90 DPSAVLPSDEAALEMYLNMIK 110
>UniRef50_Q1KSF2 Cluster: Mitochondrial branched-chain alpha-keto
acid dehydrogenase E1; n=1; Toxoplasma gondii|Rep:
Mitochondrial branched-chain alpha-keto acid
dehydrogenase E1 - Toxoplasma gondii
Length = 463
Score = 35.9 bits (79), Expect = 0.18
Identities = 16/26 (61%), Positives = 19/26 (73%)
Frame = +2
Query: 263 MVQLSHMDKIIYESQRQGRISFYMTN 340
MV+LS D + Y QRQGRISFY+ N
Sbjct: 123 MVKLSIWDNMFYSVQRQGRISFYIQN 148
>UniRef50_Q6B8N3 Cluster: Conserved hypothetical plastid protein;
n=1; Gracilaria tenuistipitata var. liui|Rep: Conserved
hypothetical plastid protein - Gracilaria tenuistipitata
var. liui (Red alga)
Length = 444
Score = 31.9 bits (69), Expect = 3.0
Identities = 14/67 (20%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
Frame = +3
Query: 129 EMKFFNETSYEPIPIYPVMDNNGQIIDLNEEPNL-DKATLINMSKPWYN*AIWIKLYMNH 305
++ F + ++P+P++ V +N+ QI+ + N+ + + ++ WY + K N
Sbjct: 144 QVTFLKQLKHQPLPLFTVTNNSNQIVLADSTENIANNSNFLDKIHKWY----YNKFLFND 199
Query: 306 KDKAAFH 326
K +A ++
Sbjct: 200 KKQAIYY 206
>UniRef50_Q1G3P8 Cluster: Putative uncharacterized protein; n=1;
Arabidopsis thaliana|Rep: Putative uncharacterized
protein - Arabidopsis thaliana (Mouse-ear cress)
Length = 218
Score = 31.9 bits (69), Expect = 3.0
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = -2
Query: 137 FHFTQVRCSCARKFRLSYHRHYVPFR 60
F + ++R C+ FRL++HR+Y P+R
Sbjct: 70 FQYERLRRICSSCFRLTHHRNYCPYR 95
>UniRef50_Q22X19 Cluster: Cation channel family protein; n=2;
Alveolata|Rep: Cation channel family protein -
Tetrahymena thermophila SB210
Length = 3020
Score = 31.1 bits (67), Expect = 5.3
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +3
Query: 177 PVMDNNGQIIDLNEEPNLDKATLINMSKPWYN*AIWIKLYMN 302
P+ +NN I N NL+K+ + N K Y ++ +KLY N
Sbjct: 362 PLNNNNNNNITNNNNNNLNKSIITNKDKSSYFNSLKLKLYQN 403
>UniRef50_A3LW72 Cluster: Predicted protein; n=1; Pichia
stipitis|Rep: Predicted protein - Pichia stipitis
(Yeast)
Length = 493
Score = 31.1 bits (67), Expect = 5.3
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = +3
Query: 117 PYLSEMK----FFNETSYEPIPIYPVMDNNGQIIDLNEEPNLD 233
PYLS K F Y IP+ PV+D NG+ I ++++ + D
Sbjct: 215 PYLSSFKKNSPFMVFAKYREIPLKPVLDANGKDISIDKDTDPD 257
>UniRef50_Q5FBD1 Cluster: Serine/threonine protein kinase ARAF;
n=10; Deuterostomia|Rep: Serine/threonine protein kinase
ARAF - Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 608
Score = 30.7 bits (66), Expect = 6.9
Identities = 14/42 (33%), Positives = 24/42 (57%), Gaps = 4/42 (9%)
Frame = -2
Query: 209 VDDLPVIIHNWINRNWF---ITSFVEEFHFTQVRC-SCARKF 96
+DD+P+ +HN++ + +F F +F F RC +C KF
Sbjct: 95 LDDVPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKF 136
>UniRef50_A0C038 Cluster: Chromosome undetermined scaffold_14, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_14,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 937
Score = 30.7 bits (66), Expect = 6.9
Identities = 17/44 (38%), Positives = 26/44 (59%), Gaps = 2/44 (4%)
Frame = +3
Query: 135 KFFNETSYEPIPIYPVMDNNGQIIDL--NEEPNLDKATLINMSK 260
+F + + E +PI ++ NGQI+ L + E +LDK I MSK
Sbjct: 258 QFLDMLNIEGLPIAIIIGKNGQIVHLGYHFEADLDKLITIEMSK 301
>UniRef50_Q6BIN7 Cluster: Similar to tr|Q96VL6 Candida albicans
Phosphatidylinositol 3; n=1; Debaryomyces hansenii|Rep:
Similar to tr|Q96VL6 Candida albicans
Phosphatidylinositol 3 - Debaryomyces hansenii (Yeast)
(Torulaspora hansenii)
Length = 2276
Score = 30.7 bits (66), Expect = 6.9
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +3
Query: 168 PIYPVMDNNGQIIDLNEEPNLDKATLINMSK 260
PI + DN ++D+N+E +LDK L + K
Sbjct: 1791 PIVEIYDNIEDVVDVNDETDLDKKNLKRLQK 1821
>UniRef50_A5E9P6 Cluster: Putative branched-chain amino acid
aminotransferase; n=1; Bradyrhizobium sp. BTAi1|Rep:
Putative branched-chain amino acid aminotransferase -
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Length = 363
Score = 30.3 bits (65), Expect = 9.2
Identities = 18/40 (45%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = -3
Query: 118 GALAPGNSGYLTTA-IMCRFGPRTGRQKPS-SPYKSI*FD 5
GAL G Y+T A ++CR+ TG +KPS PY ++ FD
Sbjct: 15 GALGFGK--YMTNAMVLCRYSDGTGWEKPSLVPYGTLAFD 52
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 356,731,955
Number of Sequences: 1657284
Number of extensions: 6340606
Number of successful extensions: 13691
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 13468
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13690
length of database: 575,637,011
effective HSP length: 89
effective length of database: 428,138,735
effective search space used: 10703468375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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