BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0406 (522 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 28 0.97 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 1.7 SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 27 2.2 SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schiz... 26 3.0 SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 25 5.2 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 27.9 bits (59), Expect = 0.97 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = +2 Query: 314 KSIFLHALNVINTKNINERELLIHLPVVRRLLSLHGKLPLPCIFLKG 454 KS + ++N ++ E++ +PV+RR L K + I KG Sbjct: 47 KSTISKQIKILNKNGFSQEEIMTFIPVIRRNLLESAKTLVKIIVQKG 93 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 27.1 bits (57), Expect = 1.7 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = +2 Query: 320 IFLHALNVINTKNINERELL--IHLPVVRRLLSL 415 +F HAL ++ K IN+RELL P+ LL L Sbjct: 870 LFDHALLIVKPKTINKRELLKVFQRPIPLLLLQL 903 >SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 287 Score = 26.6 bits (56), Expect = 2.2 Identities = 10/24 (41%), Positives = 19/24 (79%) Frame = +2 Query: 104 DLHLLQMTEFINTLRI*FPNISFM 175 +LH+L +F++TL + FP+I+F+ Sbjct: 109 NLHMLFGIDFVSTLVVSFPHITFL 132 >SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 451 Score = 26.2 bits (55), Expect = 3.0 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = +2 Query: 314 KSIFLHALNVINTKNINERELLIHLPVVRRLLSLHGKLPLPC 439 K+ + A N I T INE L HLP + L+ K+ L C Sbjct: 250 KTDYPAACNAIETLLINEAVLSSHLPKIAETLT-EAKVTLKC 290 >SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosaccharomyces pombe|chr 3|||Manual Length = 1213 Score = 25.4 bits (53), Expect = 5.2 Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 1/47 (2%) Frame = +1 Query: 367 KGTSNS-FTGGKTSFESAWEITTALYIFERRSCCTATTETLRTLVSR 504 K SN F K FE +T + + SC TTE LR+++ R Sbjct: 333 KALSNQKFRDFKHKFEDVGILTGDVQVNPEGSCLLMTTEILRSMLYR 379 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,025,174 Number of Sequences: 5004 Number of extensions: 39934 Number of successful extensions: 72 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 72 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 72 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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