BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0377 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 156 3e-39 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 151 6e-38 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 81 2e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 57 2e-09 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.17 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.2 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 3.6 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 3.6 SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 26 3.6 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.8 SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 25 6.3 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 6.3 SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 25 8.3 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 25 8.3 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 25 8.3 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 156 bits (378), Expect = 3e-39 Identities = 72/92 (78%), Positives = 80/92 (86%) Frame = +2 Query: 2 NLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQ 181 NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE Sbjct: 226 NLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHES 285 Query: 182 LSVAEITNACFEPANQMVKCDPRHASTWLAVC 277 SV EITN CFEP NQMVKCDPR A ++A C Sbjct: 286 NSVQEITNQCFEPYNQMVKCDPR-AGRYMATC 316 Score = 134 bits (324), Expect = 9e-33 Identities = 62/103 (60%), Positives = 73/103 (70%) Frame = +1 Query: 244 PPSCKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 423 P + +YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G Sbjct: 307 PRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGS 366 Query: 424 DLAKGQRAVCMLSNTTGIAEXXXXXXXXXXXXXXXKRAFVHWY 552 ++AK RAVCMLSNTT IAE KRAFVHWY Sbjct: 367 EIAKVDRAVCMLSNTTSIAE-AWSRLDHKFDLMYSKRAFVHWY 408 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 151 bits (367), Expect = 6e-38 Identities = 70/92 (76%), Positives = 78/92 (84%) Frame = +2 Query: 2 NLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQ 181 NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE Sbjct: 230 NLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHES 289 Query: 182 LSVAEITNACFEPANQMVKCDPRHASTWLAVC 277 SV EITN CFEP NQMVKCDPR ++A C Sbjct: 290 NSVQEITNQCFEPYNQMVKCDPR-TGRYMATC 320 Score = 138 bits (335), Expect = 4e-34 Identities = 63/103 (61%), Positives = 75/103 (72%) Frame = +1 Query: 244 PPSCKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 423 P + +YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG Sbjct: 311 PRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGS 370 Query: 424 DLAKGQRAVCMLSNTTGIAEXXXXXXXXXXXXXXXKRAFVHWY 552 +AK RAVCMLSNTT IAE KRAFVHWY Sbjct: 371 GIAKVNRAVCMLSNTTSIAE-AWSRLDHKFDLMYSKRAFVHWY 412 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 80.6 bits (190), Expect = 2e-16 Identities = 34/84 (40%), Positives = 53/84 (63%) Frame = +2 Query: 2 NLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQ 181 +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + + Sbjct: 224 DLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQA 283 Query: 182 LSVAEITNACFEPANQMVKCDPRH 253 +SV E+T F+ N MV DPRH Sbjct: 284 VSVPELTQQMFDANNMMVAADPRH 307 Score = 44.4 bits (100), Expect = 1e-05 Identities = 28/103 (27%), Positives = 46/103 (44%) Frame = +1 Query: 244 PPSCKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 423 P +Y+ L+RG V K+V+ I +++TK + FV+W P + PP Sbjct: 305 PRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK----- 359 Query: 424 DLAKGQRAVCMLSNTTGIAEXXXXXXXXXXXXXXXKRAFVHWY 552 DL + + + N+T I E ++AF+HWY Sbjct: 360 DL---KMSATFIGNSTSIQE-IFRRLGDQFSAMFRRKAFLHWY 398 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 56.8 bits (131), Expect = 2e-09 Identities = 28/92 (30%), Positives = 49/92 (53%), Gaps = 3/92 (3%) Frame = +2 Query: 8 NRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---AEKAYHE 178 N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P + E Sbjct: 229 NQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIR 288 Query: 179 QLSVAEITNACFEPANQMVKCDPRHASTWLAV 274 + +V ++ P NQMV +P S ++++ Sbjct: 289 KTTVLDVMRRLLLPKNQMVSVNPSKKSCFISI 320 Score = 33.1 bits (72), Expect = 0.031 Identities = 13/54 (24%), Positives = 30/54 (55%) Frame = +1 Query: 250 SCKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTV 411 SC +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + Sbjct: 315 SC-FISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYI 367 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 30.7 bits (66), Expect = 0.17 Identities = 16/45 (35%), Positives = 21/45 (46%) Frame = +2 Query: 284 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 418 VV P + RP++P P LS V PV+ V + PP P Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 Score = 25.4 bits (53), Expect = 6.3 Identities = 18/67 (26%), Positives = 22/67 (32%) Frame = +2 Query: 218 PANQMVKCDPRHASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTT 397 P V P S VV P + RP++P P S PV V + Sbjct: 620 PQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSV 679 Query: 398 SHPPWCP 418 PP P Sbjct: 680 PQPPAVP 686 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +3 Query: 84 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 230 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 3.6 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 147 QSSLPRRPTMNSFPSPRSQTHASSPPTR 230 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 3.6 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +2 Query: 320 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 406 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 26.2 bits (55), Expect = 3.6 Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 9/66 (13%) Frame = -3 Query: 366 TPVDELDSTLGFDGSDGR--------VHILGYDVTTVQHTASHVLA*RGSHFTIWLAG-S 214 TP + + F GS+G+ V++ + VQH +H L+ F +W+ G S Sbjct: 365 TPNNAVAMCWSFQGSEGQLGISLSRPVYVTNVTIEHVQHKIAHDLSSAPKDFELWVQGMS 424 Query: 213 KHAFVI 196 FV+ Sbjct: 425 SKMFVL 430 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.8 bits (54), Expect = 4.8 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = +3 Query: 81 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 221 SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664 >SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1689 Score = 25.4 bits (53), Expect = 6.3 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = -1 Query: 533 RFGVHEVELVVKGEPKVSAMPVVLDNM 453 +FG+++V+ V K K PV+LDN+ Sbjct: 15 KFGIYDVDDVEKISVKQIVNPVLLDNL 41 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 6.3 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +2 Query: 317 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 412 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 25.0 bits (52), Expect = 8.3 Identities = 15/37 (40%), Positives = 16/37 (43%) Frame = +2 Query: 299 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 409 PR LP K VL T + VSR TTS P Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 25.0 bits (52), Expect = 8.3 Identities = 7/13 (53%), Positives = 10/13 (76%) Frame = -2 Query: 439 DLWPSRLRAPRWV 401 D WPS++ P+WV Sbjct: 157 DSWPSKITLPKWV 169 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.0 bits (52), Expect = 8.3 Identities = 16/49 (32%), Positives = 29/49 (59%) Frame = +3 Query: 108 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVM 254 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVL 176 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,692,471 Number of Sequences: 5004 Number of extensions: 58136 Number of successful extensions: 239 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 213 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 233 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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