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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0357
         (668 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   210   1e-55
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   116   4e-27
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   107   1e-24
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    94   2e-20
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    62   5e-11
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    58   1e-09
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    49   7e-07
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    34   0.016
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    31   0.11 
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p...    29   0.61 
SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizos...    27   1.8  
SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|...    27   2.4  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    27   3.2  
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   4.3  
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    26   5.6  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  210 bits (514), Expect = 1e-55
 Identities = 99/133 (74%), Positives = 102/133 (76%)
 Frame = +2

Query: 254 GQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 433
           GQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE
Sbjct: 48  GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107

Query: 434 APLNPKANREKMTQIMFETFNTPAMTCHPSXXXXXXXXXXXXXXXXDSRDSVFHTVPIYE 613
           APLNPK+NREKMTQI+FETFN PA                      DS D V HTVPIYE
Sbjct: 108 APLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYE 167

Query: 614 GYALPHAILAFGL 652
           GYALPHAI+   L
Sbjct: 168 GYALPHAIMRLDL 180



 Score = 96.7 bits (230), Expect = 3e-21
 Identities = 41/49 (83%), Positives = 47/49 (95%)
 Frame = +3

Query: 117 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMDRR 263
           +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGM ++
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQK 50


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  116 bits (278), Expect = 4e-27
 Identities = 53/128 (41%), Positives = 78/128 (60%), Gaps = 1/128 (0%)
 Frame = +2

Query: 257 QKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTE 433
           QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L   PEEHP+LLTE
Sbjct: 53  QKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTE 112

Query: 434 APLNPKANREKMTQIMFETFNTPAMTCHPSXXXXXXXXXXXXXXXXDSRDSVFHTVPIYE 613
            PL    N+EK+ +  +ET N PA++                    +  D + H+VPIY+
Sbjct: 113 PPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYD 172

Query: 614 GYALPHAI 637
           G+++P AI
Sbjct: 173 GFSIPSAI 180



 Score = 55.2 bits (127), Expect = 8e-09
 Identities = 22/37 (59%), Positives = 29/37 (78%)
 Frame = +3

Query: 135 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 245
           + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM
Sbjct: 12  ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVM 48


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  107 bits (257), Expect = 1e-24
 Identities = 49/133 (36%), Positives = 75/133 (56%)
 Frame = +2

Query: 239 RDGRYGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 418
           + G    KD  VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P    
Sbjct: 43  KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102

Query: 419 VLLTEAPLNPKANREKMTQIMFETFNTPAMTCHPSXXXXXXXXXXXXXXXXDSRDSVFHT 598
           +LLTE P+NP ANREKM + MFE +    +                     DS D V H 
Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHI 162

Query: 599 VPIYEGYALPHAI 637
           VP+YE   L H +
Sbjct: 163 VPVYESVVLNHLV 175



 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 21/35 (60%), Positives = 26/35 (74%)
 Frame = +3

Query: 123 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 227
           E A +V+DNG+G  K G+A D+ PR  FPSIVGRP
Sbjct: 2   ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 93.9 bits (223), Expect = 2e-20
 Identities = 49/134 (36%), Positives = 66/134 (49%), Gaps = 9/134 (6%)
 Frame = +2

Query: 263 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 439
           D ++G++A  K     +L YPI HG + NWD ME+ W  + +  LR  PE+H  LLTE P
Sbjct: 73  DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132

Query: 440 LNPKANREKMTQIMFETFN--------TPAMTCHPSXXXXXXXXXXXXXXXXDSRDSVFH 595
           LNP  NRE   +IMFE+FN           +    S                DS D V H
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192

Query: 596 TVPIYEGYALPHAI 637
            +P+ EGY +  +I
Sbjct: 193 IIPVAEGYVIGSSI 206



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 14/28 (50%), Positives = 23/28 (82%)
 Frame = +3

Query: 135 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 218
           +++DNG+G  K G+AG+DAP  VFP+++
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 62.5 bits (145), Expect = 5e-11
 Identities = 29/91 (31%), Positives = 54/91 (59%), Gaps = 1/91 (1%)
 Frame = +2

Query: 239 RDGRYGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 415
           RD +  +  + VG++   + G  ++ + P E  +++NWD ME++  +TF  +L +   EH
Sbjct: 58  RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116

Query: 416 PVLLTEAPLNPKANREKMTQIMFETFNTPAM 508
           P+ +TE   NP   R  MT+++FE +N P++
Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSV 147



 Score = 35.9 bits (79), Expect = 0.005
 Identities = 15/32 (46%), Positives = 22/32 (68%)
 Frame = +3

Query: 135 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 230
           LV+DNGS   +AG+ G+  P+ VF ++V R R
Sbjct: 27  LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 26/67 (38%), Positives = 37/67 (55%)
 Frame = +2

Query: 305 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 484
           + +K  I +G V NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + MF
Sbjct: 63  MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122

Query: 485 ETFNTPA 505
           E+   PA
Sbjct: 123 ESLRCPA 129



 Score = 40.3 bits (90), Expect = 2e-04
 Identities = 16/34 (47%), Positives = 24/34 (70%)
 Frame = +3

Query: 120 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 221
           +EV+A+V+D GS   + GF+G+D P+ V PS  G
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 24/84 (28%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
 Frame = +2

Query: 260 KDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 433
           ++ YV DE Q    I  + +K    +GI+ +W+     W      +L+V P E+ +++TE
Sbjct: 49  RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108

Query: 434 APLNPKANREKMTQIMFETFNTPA 505
              NP++ R+++ +  FE  + PA
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPA 132



 Score = 36.7 bits (81), Expect = 0.003
 Identities = 14/34 (41%), Positives = 23/34 (67%)
 Frame = +3

Query: 120 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 221
           EE+ +LV+D GS   + G+AG+++P  + PS  G
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 34.3 bits (75), Expect = 0.016
 Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 4/68 (5%)
 Frame = +2

Query: 320 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 487
           PI+ G V +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 488 TFNTPAMT 511
               P  T
Sbjct: 172 ECQVPGFT 179


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 31.5 bits (68), Expect = 0.11
 Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
 Frame = +2

Query: 380  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTP 502
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ NTP
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441


>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 277

 Score = 29.1 bits (62), Expect = 0.61
 Identities = 19/66 (28%), Positives = 29/66 (43%)
 Frame = -2

Query: 352 IPVSDDSVFDGVFEGQDTSFALCLISYIRVLLSIPTITP*WRGLPTIEGNTARGASSPAK 173
           +P  + +V        +TSFAL   S    L   PT +      P +E +    +S+PA 
Sbjct: 63  LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122

Query: 172 PALHIP 155
           P L+ P
Sbjct: 123 PQLNSP 128


>SPCC1442.07c |||ubiquitin/metalloprotease fusion
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 282

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = -1

Query: 290 SVPHLLHKSPSVHTDHHALMAGPSHDRGKH 201
           +VP L    P+ HT H +   G +H++G H
Sbjct: 148 TVPLLSEMDPAEHTRHDSKTLGLNHNQGAH 177


>SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase
           Rqh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1328

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = +1

Query: 586 CFPHRAHLRGIRTSPRHSCVWTLAGRD 666
           C  H+ HL+G R +   +   TL+G+D
Sbjct: 510 CLKHKFHLKGFRKNQLEAINGTLSGKD 536


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 15/45 (33%), Positives = 18/45 (40%)
 Frame = -2

Query: 250 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 116
           PT+ P     P    N     S P         PL+TTN  TS+S
Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +2

Query: 422 LLTEAPLNPKANREKMTQIMFETFN 496
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = -3

Query: 162 TYRSHCLLPTRQLLRRTS*ISEI 94
           TYR H  +PT  LL  T  +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,035,514
Number of Sequences: 5004
Number of extensions: 66363
Number of successful extensions: 191
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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