BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0346 (608 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.23 SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 29 0.40 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 2.8 SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 2.8 SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 26 5.0 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 6.5 SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 25 8.7 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 30.3 bits (65), Expect = 0.23 Identities = 21/67 (31%), Positives = 34/67 (50%) Frame = -2 Query: 205 GRVHSPPDVK*LLEPIVILNLNEPPTLNVDSQEVLSPVITQIIILRVSFLLHDVIPSDPP 26 G V SPP+ PI +++N P +N S+ L P +++ ++ + P PP Sbjct: 262 GTVSSPPNSP--PRPIAPVSMN--PAINSTSKPPLPPPSSRVSAAALA--ANKKRPPPPP 315 Query: 25 PPNRRQR 5 PP+RR R Sbjct: 316 PPSRRNR 322 >SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1315 Score = 29.5 bits (63), Expect = 0.40 Identities = 14/42 (33%), Positives = 27/42 (64%), Gaps = 4/42 (9%) Frame = -2 Query: 133 PTLNVDSQEVLSPVITQIIIL-RVSFLLHDVIPSDP---PPP 20 PT N+ S+ +LSP+I QI+++ + ++ ++ +P PPP Sbjct: 1129 PTANLVSKRILSPLIGQIVLICIIQYITLRIVRREPWYIPPP 1170 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 26.6 bits (56), Expect = 2.8 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%) Frame = +1 Query: 274 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 438 P P+ S ++ H + + +L N I+L ++PLSP A+ P+PI L + Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231 Query: 439 SCA 447 S A Sbjct: 232 SDA 234 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 26.6 bits (56), Expect = 2.8 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +3 Query: 381 PPFASWRNSEEARTDRPSQQLRSLN 455 PP AS RN E + PS+Q S+N Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377 >SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 2052 Score = 25.8 bits (54), Expect = 5.0 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = +3 Query: 510 VKSAHFLTNRPKSAKSLINQKNRP 581 VK FLTN + SL+ Q NRP Sbjct: 675 VKDYDFLTNLNATTLSLLTQSNRP 698 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.4 bits (53), Expect = 6.5 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = -2 Query: 139 EPPTLNVDSQEVLSPVIT 86 EPPT++V S LSP +T Sbjct: 1034 EPPTVSVPSPSALSPSVT 1051 >SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 603 Score = 25.0 bits (52), Expect = 8.7 Identities = 11/25 (44%), Positives = 14/25 (56%) Frame = +3 Query: 105 TSCESTFKVGGSFKLRMTMGSSNYL 179 T STFK+ G L + + S NYL Sbjct: 174 TDSNSTFKLTGDTSLALNVSSYNYL 198 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,510,803 Number of Sequences: 5004 Number of extensions: 48876 Number of successful extensions: 95 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 95 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 95 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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