BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0340 (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 176 3e-45 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 101 7e-23 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 97 2e-21 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 93 2e-20 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 64 2e-11 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 62 6e-11 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 50 3e-07 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 35 0.011 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.079 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 28 0.97 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.3 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 3.0 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.9 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.9 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 5.2 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 5.2 SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 25 6.9 SPBC3D6.02 |but2||But2 family protein But2 |Schizosaccharomyces ... 25 9.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 176 bits (428), Expect = 3e-45 Identities = 79/91 (86%), Positives = 83/91 (91%) Frame = +2 Query: 236 GTEGLLLGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 415 G + +GDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE Sbjct: 48 GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107 Query: 416 APLNPKANREKMTQIMFETFNTPAMYVAIQA 508 APLNPK+NREKMTQI+FETFN PA YVAIQA Sbjct: 108 APLNPKSNREKMTQIIFETFNAPAFYVAIQA 138 Score = 109 bits (261), Expect = 4e-25 Identities = 47/52 (90%), Positives = 51/52 (98%) Frame = +3 Query: 99 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSY 254 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSY Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSY 53 Score = 70.5 bits (165), Expect = 2e-13 Identities = 33/43 (76%), Positives = 36/43 (83%) Frame = +1 Query: 505 SLLSLYAXGRTTRIVLDFRDGVFHTVPIYEGYALPHAILRFGL 633 ++LSLYA GRTT IVLD DGV HTVPIYEGYALPHAI+R L Sbjct: 138 AVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDL 180 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 101 bits (243), Expect = 7e-23 Identities = 41/96 (42%), Positives = 69/96 (71%) Frame = +2 Query: 221 RDGRYGTEGLLLGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 400 + G + +++GDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 401 VLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQA 508 +LLTE P+NP ANREKM + MFE + +YVAIQA Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQA 138 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +3 Query: 105 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 209 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 Score = 43.2 bits (97), Expect = 3e-05 Identities = 19/40 (47%), Positives = 27/40 (67%) Frame = +1 Query: 505 SLLSLYAXGRTTRIVLDFRDGVFHTVPIYEGYALPHAILR 624 ++LSLYA G ++ +V+D DGV H VP+YE L H + R Sbjct: 138 AVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGR 177 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 96.7 bits (230), Expect = 2e-21 Identities = 47/114 (41%), Positives = 65/114 (57%), Gaps = 1/114 (0%) Frame = +2 Query: 254 LGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 430 +G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP Sbjct: 76 IGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNP 135 Query: 431 KANREKMTQIMFETFNTPAMYVAIQACSRCTRXVVPPVSCWTSATVSSTPCPST 592 NRE +IMFE+FN +Y+A+QA V+ + WTS+ V+ T Sbjct: 136 PENRENTAEIMFESFNCAGLYIAVQA-------VLALAASWTSSKVTDRSLTGT 182 Score = 41.9 bits (94), Expect = 7e-05 Identities = 20/48 (41%), Positives = 30/48 (62%), Gaps = 2/48 (4%) Frame = +3 Query: 117 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSY 254 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSY 54 Score = 29.5 bits (63), Expect = 0.42 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +1 Query: 538 TRIVLDFRDGVFHTVPIYEGYALPHAI 618 T V+D DGV H +P+ EGY + +I Sbjct: 180 TGTVVDSGDGVTHIIPVAEGYVIGSSI 206 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 93.5 bits (222), Expect = 2e-20 Identities = 40/87 (45%), Positives = 60/87 (68%), Gaps = 1/87 (1%) Frame = +2 Query: 248 LLLGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPL 424 + +G EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL Sbjct: 56 MFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115 Query: 425 NPKANREKMTQIMFETFNTPAMYVAIQ 505 N+EK+ + +ET N PA+ ++Q Sbjct: 116 ANIRNKEKIAEYFYETLNVPALSFSLQ 142 Score = 59.7 bits (138), Expect = 3e-10 Identities = 25/46 (54%), Positives = 33/46 (71%) Frame = +3 Query: 117 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSY 254 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMF 57 Score = 49.6 bits (113), Expect = 4e-07 Identities = 21/37 (56%), Positives = 30/37 (81%) Frame = +1 Query: 508 LLSLYAXGRTTRIVLDFRDGVFHTVPIYEGYALPHAI 618 +L+LYA RTT IVL+ DG+ H+VPIY+G+++P AI Sbjct: 144 VLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAI 180 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 64.1 bits (149), Expect = 2e-11 Identities = 34/94 (36%), Positives = 48/94 (51%), Gaps = 1/94 (1%) Frame = +2 Query: 227 GRYGTEGLLLGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPV 403 G + L G+E K + +K I +G V NWD +W + +L+ P EHP+ Sbjct: 42 GEFSDGRRLFGEEYIYKSNPGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPI 101 Query: 404 LLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 505 L+TE NP NR K + MFE+ PA Y+A Q Sbjct: 102 LITEPFDNPPENRVKTLETMFESLRCPATYLAKQ 135 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 102 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 203 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 62.1 bits (144), Expect = 6e-11 Identities = 30/95 (31%), Positives = 54/95 (56%), Gaps = 1/95 (1%) Frame = +2 Query: 221 RDGRYGTEGLLLGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 397 RD + L+G++ + G ++ + P E +++NWD ME++ +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 Query: 398 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAI 502 P+ +TE NP R MT+++FE +N P++ I Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 117 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 212 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 50.0 bits (114), Expect = 3e-07 Identities = 20/66 (30%), Positives = 38/66 (57%) Frame = +2 Query: 311 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 490 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 491 YVAIQA 508 Y+ QA Sbjct: 134 YLTKQA 139 Score = 36.7 bits (81), Expect = 0.003 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +3 Query: 102 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 203 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 34.7 bits (76), Expect = 0.011 Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%) Frame = +2 Query: 302 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 469 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 470 TFNTPAMYVAIQ 505 P +A + Sbjct: 172 ECQVPGFTIAYE 183 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.079 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +2 Query: 362 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 505 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 28.3 bits (60), Expect = 0.97 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -1 Query: 334 IPVSDDSVFDGVFEGQDTSFALCLIS**ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 155 +P + +V +TSFAL S S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 154 PALHIP 137 P L+ P Sbjct: 123 PQLNSP 128 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.1 bits (57), Expect = 2.3 Identities = 15/40 (37%), Positives = 21/40 (52%) Frame = +2 Query: 458 IMFETFNTPAMYVAIQACSRCTRXVVPPVSCWTSATVSST 577 + F+ T + Y +QACS CT + S S T+SST Sbjct: 94 LSFDGGETYSQYFTLQACSTCT---ISTSSLSYSGTISST 130 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 3.0 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -1 Query: 232 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 98 PT+ P P N S P PL+TTN TS+S Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 404 LLTEAPLNPKANREKMTQIMFETFN 478 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.9 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -1 Query: 238 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 146 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 25.8 bits (54), Expect = 5.2 Identities = 16/58 (27%), Positives = 23/58 (39%), Gaps = 1/58 (1%) Frame = +1 Query: 397 PSPAH*GSPQPQGQQREDDPDHVRNIQHARHVRRHPSLLSLYAXGRTTRIV-LDFRDG 567 P P S QP Q P H R +++ S S++ +IV L +DG Sbjct: 61 PLPFRSTSLQPPSSQPPAPPTHQREASPVKNIEHSESFPSVFGTSNNHQIVPLTLKDG 118 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -2 Query: 255 NKSPSVPYRPSRPDGGAFPRSRGTR 181 +K+PS PY SRP A S TR Sbjct: 311 SKAPSYPYSSSRPSASASLASSPTR 335 >SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1016 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = +2 Query: 518 CTRXVVPPVSCWTSATVSSTPCPSTKDTHFPTP 616 C + PV +S T S TP P+T + PTP Sbjct: 833 CDNGALGPVQS-SSTTSSITPTPTTTSSITPTP 864 >SPBC3D6.02 |but2||But2 family protein But2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 390 Score = 25.0 bits (52), Expect = 9.1 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = -2 Query: 633 KSKTQNGVGKCVSFVDGHGVEDTVAEVQH 547 KSK+ K V+ +DG GV +T + + Sbjct: 31 KSKSNTNTAKSVAILDGVGVNNTFGVISY 59 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,843,268 Number of Sequences: 5004 Number of extensions: 62402 Number of successful extensions: 198 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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