Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0324
(672 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella ve... 137 2e-31
UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon nigrovirid... 133 3e-30
UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondr... 131 1e-29
UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondri... 129 5e-29
UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 128 1e-28
UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63; Euka... 128 1e-28
UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Ma... 126 6e-28
UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52; cell... 124 2e-27
UniRef50_A0L5P5 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 120 3e-26
UniRef50_A7CWP9 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 118 1e-25
UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|R... 118 2e-25
UniRef50_Q0AIF8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 118 2e-25
UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplas... 116 7e-25
UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole geno... 111 1e-23
UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2; Ostre... 106 6e-22
UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187; cell... 102 7e-21
UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:... 100 4e-20
UniRef50_A7IMB8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 98 2e-19
UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic... 98 2e-19
UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila melanogas... 97 3e-19
UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,... 96 8e-19
UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus californi... 96 8e-19
UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isofo... 96 8e-19
UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;... 94 2e-18
UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;... 94 3e-18
UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Mali... 93 6e-18
UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2; Ostr... 92 1e-17
UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Ma... 89 9e-17
UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 89 1e-16
UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor circinelloides... 88 2e-16
UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:... 87 3e-16
UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep: ... 87 4e-16
UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10; Franc... 85 1e-15
UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 85 1e-15
UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6; Deinococci|... 84 3e-15
UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep... 83 8e-15
UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase (... 80 4e-14
UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2; ... 76 9e-13
UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20; Trypanoso... 75 2e-12
UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:... 74 3e-12
UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 73 8e-12
UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2; Aeromo... 69 8e-11
UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia intestinalis... 69 1e-10
UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme; ... 68 2e-10
UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2; Mucoromyco... 68 2e-10
UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|R... 68 2e-10
UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococc... 68 2e-10
UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3; Schizo... 62 9e-09
UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Mali... 61 2e-08
UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep: ... 56 8e-07
UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 56 1e-06
UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:... 55 2e-06
UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep: ... 54 2e-06
UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium globosum|... 53 5e-06
UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n... 51 2e-05
UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 50 4e-05
UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondri... 50 5e-05
UniRef50_Q9S4T5 Cluster: NAD-malate oxidoreductase homolog; n=15... 48 2e-04
UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n... 45 0.002
UniRef50_UPI0000DB7FF6 Cluster: PREDICTED: hypothetical protein,... 42 0.010
UniRef50_A5C6I9 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep: ... 39 0.13
UniRef50_UPI0000DA40E4 Cluster: PREDICTED: hypothetical protein;... 34 2.7
UniRef50_Q4SHK3 Cluster: Chromosome 5 SCAF14581, whole genome sh... 34 3.6
UniRef50_A1CKF3 Cluster: Stress response protein (Ish1), putativ... 34 3.6
UniRef50_Q4RNM6 Cluster: Chromosome 21 SCAF15012, whole genome s... 33 4.8
UniRef50_UPI0001560FE5 Cluster: PREDICTED: similar to KIAA2007 p... 33 6.3
UniRef50_Q7XJP0 Cluster: SNF2/SWI2 family global transcription f... 33 6.3
UniRef50_Q7QQ11 Cluster: GLP_227_10956_9892; n=1; Giardia lambli... 33 6.3
UniRef50_Q1EI20 Cluster: Putative uncharacterized protein; n=2; ... 33 8.3
UniRef50_A0WC26 Cluster: Multi-sensor hybrid histidine kinase pr... 33 8.3
>UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 365
Score = 137 bits (332), Expect = 2e-31
Identities = 57/85 (67%), Positives = 74/85 (87%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI LM LL+RNE LF+R + D E+MPIVYTPTVGLAC+K+G+++RRPRGLFI+IHDK
Sbjct: 71 KYIQLMALLERNESLFFRVLFDYTEELMPIVYTPTVGLACRKYGMIFRRPRGLFISIHDK 130
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GH+ D++ NWP T+V+AIV+TDGER
Sbjct: 131 GHIRDIVSNWPTTEVKAIVMTDGER 155
Score = 60.5 bits (140), Expect = 4e-08
Identities = 29/57 (50%), Positives = 38/57 (66%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
+ G D ++ LNKG+AFT+EERQ+LGIHGLLPP V +QE Q + + R N L
Sbjct: 13 IRGTDIMRDSHLNKGLAFTLEERQILGIHGLLPPCVISQEIQAQRVYRELQRKPNDL 69
Score = 49.2 bits (112), Expect = 9e-05
Identities = 21/22 (95%), Positives = 21/22 (95%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYT 574
LGLGDLG CGMGIPVGKLALYT
Sbjct: 157 LGLGDLGCCGMGIPVGKLALYT 178
>UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 694
Score = 133 bits (322), Expect = 3e-30
Identities = 56/85 (65%), Positives = 71/85 (83%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI LM L DRNE LFYR + ++ + MPIVYTPTVGLACQ++GL +RRPRGLFITIHD+
Sbjct: 118 KYILLMTLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDR 177
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GH+ +L +WPE D++A+VVTDGER
Sbjct: 178 GHIATMLNSWPEEDIKAVVVTDGER 202
Score = 60.9 bits (141), Expect = 3e-08
Identities = 31/72 (43%), Positives = 43/72 (59%)
Frame = +1
Query: 52 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSID 231
+ +G + G D ++P LNKGMAFT+EER +GIHGLLPP +Q+ QV S +
Sbjct: 51 ASEGSVRTKKRGYDITRNPHLNKGMAFTLEERLQMGIHGLLPPCFLSQDVQVLRVMKSYE 110
Query: 232 RYENPLNNTSIL 267
NPL+ +L
Sbjct: 111 TRSNPLDKYILL 122
Score = 45.6 bits (103), Expect = 0.001
Identities = 22/29 (75%), Positives = 23/29 (79%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTALRRHQA 595
LGLGDLG+ GMGIPVGKLAL LRR A
Sbjct: 204 LGLGDLGSYGMGIPVGKLALLHRLRRRAA 232
>UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondrial
precursor; n=15; Bilateria|Rep: NADP-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 604
Score = 131 bits (317), Expect = 1e-29
Identities = 57/85 (67%), Positives = 70/85 (82%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI LM L DRNE LFYR + +V + MPIVYTPTVGLACQ +GL +RRPRGLFITIHDK
Sbjct: 106 KYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDK 165
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GH+ +L +WPE +++A+VVTDGER
Sbjct: 166 GHLATMLNSWPEDNIKAVVVTDGER 190
Score = 56.0 bits (129), Expect = 8e-07
Identities = 58/194 (29%), Positives = 86/194 (44%), Gaps = 8/194 (4%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNTSI 264
G D ++P LNKGMAFT+EER LGIHGL+PP +Q+ Q+ +R ++ L+ I
Sbjct: 50 GYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYII 109
Query: 265 LWGSWTAMSICSTVSSRITWLK*CQLCTLRRLAWPVRSSGLCTADQGASSSRS-----TI 429
L M++ + + + + + GL G + R TI
Sbjct: 110 L------MTLQDRNEKLFYRVLTSDVEKFMPIVY-TPTVGLACQHYGLTFRRPRGLFITI 162
Query: 430 RD---MYTMFLRTGRRRTSAPLL*PTENVFGPGRFGRLRDGHPCGQTRALHRASEASSPQ 600
D + TM A ++ E + G G G G P G+ AL+ A +PQ
Sbjct: 163 HDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKL-ALYTACGGVNPQ 221
Query: 601 SMVYPSLSNVGTNN 642
+ P L +VGTNN
Sbjct: 222 QCL-PVLLDVGTNN 234
Score = 46.4 bits (105), Expect = 6e-04
Identities = 21/23 (91%), Positives = 21/23 (91%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIPVGKLALYTA
Sbjct: 192 LGLGDLGCYGMGIPVGKLALYTA 214
>UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=53; Eumetazoa|Rep: NAD-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 584
Score = 129 bits (312), Expect = 5e-29
Identities = 52/85 (61%), Positives = 71/85 (83%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YIY+MG+ +RNE LFYR + D++ +MPIVYTPTVGLAC ++G ++RRP+GLFI+I D+
Sbjct: 81 KYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDR 140
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GHV ++ NWPE V+A+VVTDGER
Sbjct: 141 GHVRSIVDNWPENHVKAVVVTDGER 165
Score = 56.0 bits (129), Expect = 8e-07
Identities = 24/57 (42%), Positives = 38/57 (66%)
Frame = +1
Query: 103 HPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNTSILWG 273
+P NKGMAFT++ERQ+LG+ GLLPP+++TQ+ Q ++ + +PL + G
Sbjct: 31 NPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMG 87
Score = 44.8 bits (101), Expect = 0.002
Identities = 20/23 (86%), Positives = 20/23 (86%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIPVGKL LYTA
Sbjct: 167 LGLGDLGVYGMGIPVGKLCLYTA 189
>UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 216
Score = 128 bits (309), Expect = 1e-28
Identities = 54/85 (63%), Positives = 70/85 (82%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YIYL L DRNE LFY+ V ++V MP++YTPTVGLACQ++G+V+RRPRGL+ITIHD+
Sbjct: 110 RYIYLTSLQDRNEALFYKLVIEHVEYCMPLIYTPTVGLACQRYGVVFRRPRGLYITIHDR 169
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
H+ ++L NWPE V+AIV TDGER
Sbjct: 170 HHIPEILNNWPEPIVKAIVFTDGER 194
Score = 60.1 bits (139), Expect = 5e-08
Identities = 27/43 (62%), Positives = 33/43 (76%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQV 207
L G+D ++ P N+G AFT+ ERQLLGIHGLLPP V T E+QV
Sbjct: 52 LLGIDVVRDPRTNRGTAFTVNERQLLGIHGLLPPSVLTLEQQV 94
Score = 34.3 bits (75), Expect = 2.7
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVG 556
LGLGDLGA GMGIP+G
Sbjct: 196 LGLGDLGAYGMGIPIG 211
>UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63;
Eukaryota|Rep: NADP-dependent malic enzyme - Homo
sapiens (Human)
Length = 572
Score = 128 bits (309), Expect = 1e-28
Identities = 55/85 (64%), Positives = 69/85 (81%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ LM L DRNE LFYR + ++ + MPIVYTPTVGLACQ++ LV+R+PRGLFITIHD+
Sbjct: 71 RYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDR 130
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GH+ VL WPE ++AIVVTDGER
Sbjct: 131 GHIASVLNAWPEDVIKAIVVTDGER 155
Score = 51.6 bits (118), Expect = 2e-05
Identities = 23/36 (63%), Positives = 28/36 (77%)
Frame = +1
Query: 100 KHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQV 207
++P LNK +AFT+EERQ L IHGLLPP +QE QV
Sbjct: 20 RNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQV 55
Score = 46.0 bits (104), Expect = 8e-04
Identities = 21/23 (91%), Positives = 21/23 (91%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIPVGKLALYTA
Sbjct: 157 LGLGDLGCNGMGIPVGKLALYTA 179
>UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 613
Score = 126 bits (303), Expect = 6e-28
Identities = 58/124 (46%), Positives = 82/124 (66%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ +M L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL++++ DK
Sbjct: 156 RYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLKDK 215
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G V DVL+NWPE +++ IVVTDGER GG++PS L
Sbjct: 216 GKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSA-CL 274
Query: 612 PITI 623
PITI
Sbjct: 275 PITI 278
Score = 44.0 bits (99), Expect = 0.003
Identities = 23/56 (41%), Positives = 34/56 (60%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
SG L+ P NKG+AF+ +ER + GLLPP V +Q+ QV+ ++ +Y PL
Sbjct: 99 SGYTLLRDPHHNKGLAFSEKERDAHYLRGLLPPAVVSQDLQVKKIMHNLRQYSVPL 154
>UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52;
cellular organisms|Rep: NADP-dependent malic enzyme -
Mus musculus (Mouse)
Length = 572
Score = 124 bits (299), Expect = 2e-27
Identities = 55/85 (64%), Positives = 67/85 (78%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ LM L DRNE LFY + +V + MPIVYTPTVGLACQ++ L +R+PRGLFI+IHDK
Sbjct: 71 RYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDK 130
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GH+ VL WPE V+AIVVTDGER
Sbjct: 131 GHIASVLNAWPEDVVKAIVVTDGER 155
Score = 50.4 bits (115), Expect = 4e-05
Identities = 23/36 (63%), Positives = 28/36 (77%)
Frame = +1
Query: 100 KHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQV 207
+ P LNK +AFT+EERQ L IHGLLPP + +QE QV
Sbjct: 20 RDPHLNKDLAFTLEERQQLNIHGLLPPCIISQELQV 55
Score = 46.0 bits (104), Expect = 8e-04
Identities = 21/23 (91%), Positives = 21/23 (91%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIPVGKLALYTA
Sbjct: 157 LGLGDLGCNGMGIPVGKLALYTA 179
>UniRef50_A0L5P5 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Magnetococcus sp. MC-1|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Magnetococcus
sp. (strain MC-1)
Length = 556
Score = 120 bits (289), Expect = 3e-26
Identities = 51/85 (60%), Positives = 67/85 (78%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI+L GL +RNE LFYR V N+ EM+PI+YTPTVG ACQ +G ++RRP+G+FI+I+DK
Sbjct: 75 KYIFLTGLQERNETLFYRLVMTNIEEMLPIIYTPTVGKACQTYGHIFRRPQGMFISINDK 134
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + ++L NW DVR IVVTDG R
Sbjct: 135 GRIAELLGNWVHKDVRVIVVTDGSR 159
Score = 50.4 bits (115), Expect = 4e-05
Identities = 24/46 (52%), Positives = 31/46 (67%)
Frame = +1
Query: 70 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQV 207
T + L+ L P +NKG+AFT EER L + GLLPPRV+T E Q+
Sbjct: 14 TGHANPLEILNDPYMNKGVAFTEEERDLFHLRGLLPPRVQTMEAQL 59
Score = 49.2 bits (112), Expect = 9e-05
Identities = 23/24 (95%), Positives = 23/24 (95%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTAL 580
LGLGDLGA GMGIPVGKLALYTAL
Sbjct: 161 LGLGDLGAHGMGIPVGKLALYTAL 184
>UniRef50_A7CWP9 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Opitutaceae bacterium TAV2|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Opitutaceae
bacterium TAV2
Length = 561
Score = 118 bits (284), Expect = 1e-25
Identities = 69/159 (43%), Positives = 86/159 (54%), Gaps = 5/159 (3%)
Frame = +3
Query: 45 MGLRGRTAHXXXXXXXXXXXXXXXXGHGFYHRRASALG-----HTRVVTTTSQDARGAG* 209
+GL R A G F R ALG RV T Q+ R
Sbjct: 11 LGLSARGARASLRGTALLGDSVLNKGTAFSERERDALGLRGLLPPRVFTLEQQEQRALN- 69
Query: 210 TMQALDR*IRKST*QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLV 389
M I K YIYL L RNE LFYR + ++ EM+P+VYTPTVG AC ++G
Sbjct: 70 AMAKKPSAIEK----YIYLTTLQSRNETLFYRLLTNHAEEMIPLVYTPTVGQACLEYGAN 125
Query: 390 YRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 506
+RRPRGLFI+I D+G + ++L++WP TDVR IVVTDGER
Sbjct: 126 FRRPRGLFISIKDRGRIAEILRHWPITDVRMIVVTDGER 164
Score = 45.2 bits (102), Expect = 0.001
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIPVGKLALY+A
Sbjct: 166 LGLGDLGVLGMGIPVGKLALYSA 188
Score = 44.8 bits (101), Expect = 0.002
Identities = 25/58 (43%), Positives = 31/58 (53%)
Frame = +1
Query: 31 DRIGLWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQ 204
D++ G + L G L LNKG AF+ ER LG+ GLLPPRV T E+Q
Sbjct: 6 DQVSSLGLSARGARASLRGTALLGDSVLNKGTAFSERERDALGLRGLLPPRVFTLEQQ 63
>UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|Rep:
Malic enzyme - Bradyrhizobium japonicum
Length = 531
Score = 118 bits (283), Expect = 2e-25
Identities = 51/85 (60%), Positives = 67/85 (78%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ L L DRNE LF+R V DN+ E+ PI+YTPTVGLACQK+GL+++RPRG+FI+ D+
Sbjct: 56 KYVALNALHDRNEALFFRVVVDNIDEIQPIIYTPTVGLACQKYGLIFQRPRGMFISSRDR 115
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + ++LKNWP R IVVTDGER
Sbjct: 116 GQIAEILKNWP-YPARLIVVTDGER 139
Score = 46.8 bits (106), Expect = 5e-04
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = +1
Query: 97 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
L+ P LNKG AFT ER LG+ GLLPP V T E QV+
Sbjct: 4 LRDPLLNKGTAFTEAERAALGLRGLLPPCVLTMETQVD 41
Score = 44.8 bits (101), Expect = 0.002
Identities = 20/23 (86%), Positives = 22/23 (95%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLGA GMGIPVGKL+LY+A
Sbjct: 141 LGLGDLGANGMGIPVGKLSLYSA 163
>UniRef50_Q0AIF8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Nitrosomonas|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Nitrosomonas
eutropha (strain C71)
Length = 536
Score = 118 bits (283), Expect = 2e-25
Identities = 50/85 (58%), Positives = 66/85 (77%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YIYL LL+RN+ LFYR + D++ E+MP+VYTPTVG AC K ++R+P+G +IT D+
Sbjct: 62 KYIYLNDLLERNQQLFYRTLVDHIGEIMPLVYTPTVGEACVKLSHIFRKPQGFYITPEDR 121
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + LKNWPETDV+ IVVTDGER
Sbjct: 122 GEIISRLKNWPETDVQIIVVTDGER 146
Score = 43.2 bits (97), Expect = 0.006
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLGA GMGIP+GK++LY A
Sbjct: 148 LGLGDLGANGMGIPIGKISLYVA 170
Score = 33.9 bits (74), Expect = 3.6
Identities = 20/46 (43%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLP---PRVKTQEEQV 207
L G L P NK AFT EER+ G+ GLLP +K Q+++V
Sbjct: 4 LYGKTLLNDPVQNKSTAFTREEREHYGLQGLLPYGVTDIKKQQQRV 49
>UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplast
precursor; n=79; Magnoliophyta|Rep: NADP-dependent malic
enzyme, chloroplast precursor - Zea mays (Maize)
Length = 636
Score = 116 bits (278), Expect = 7e-25
Identities = 54/124 (43%), Positives = 77/124 (62%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI +M L + +E LFY+ + DNV E++P VYTPTVG ACQK+G ++ RP+GL++++ DK
Sbjct: 153 RYIAMMNLQETDERLFYKLLIDNVVELLPFVYTPTVGEACQKYGSIFGRPQGLYVSLKDK 212
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G V +VL+NWP +++ I VTDGER GG+ PS+ L
Sbjct: 213 GKVLEVLRNWPHRNIQVICVTDGERILGLGDLGCQGMGIPVGKLALYTALGGVDPSV-CL 271
Query: 612 PITI 623
PITI
Sbjct: 272 PITI 275
Score = 50.4 bits (115), Expect = 4e-05
Identities = 27/60 (45%), Positives = 37/60 (61%)
Frame = +1
Query: 70 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
TS SG L+ P NKG+AFT EER + GLLPP V +QE Q++ ++ +Y+ PL
Sbjct: 92 TSVASGYTLLRDPHHNKGLAFTEEERDGHYLRGLLPPAVLSQELQIKKFMNTLRQYQTPL 151
>UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole genome
shotgun sequence; n=3; Vitis vinifera|Rep: Chromosome
chr2 scaffold_11, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 573
Score = 111 bits (267), Expect = 1e-23
Identities = 53/124 (42%), Positives = 75/124 (60%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ +M +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++RRP+ L+I
Sbjct: 253 RYMAMMDFQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQSLYIIDFFM 312
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G + +VLKNWPE ++ IVVT+GER GG+ PS+ L
Sbjct: 313 GKILEVLKNWPERSIQVIVVTNGERILGLGDLGCQGMGIPVGKLSLYTTLGGLHPSV-CL 371
Query: 612 PITI 623
P+TI
Sbjct: 372 PVTI 375
Score = 43.2 bits (97), Expect = 0.006
Identities = 23/56 (41%), Positives = 33/56 (58%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
SG L+ P NKG+AFT +ER + GLLPP V Q+ Q + ++ +Y+ PL
Sbjct: 196 SGYTLLRDPHHNKGLAFTEKERDAHYLRGLLPPAVLNQDLQEKRLMHNLRQYKVPL 251
>UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP dependent malic enzyme -
Ostreococcus tauri
Length = 641
Score = 106 bits (254), Expect = 6e-22
Identities = 49/124 (39%), Positives = 79/124 (63%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
++ +L L +RNE LFYR V D++ E++P++ PTV C++ GL+YR+PRGL++++ DK
Sbjct: 139 KHAWLPALYERNERLFYRVVKDHLEELLPVLAEPTVWQVCREAGLMYRQPRGLYVSMQDK 198
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G VY +LKNWP +V+A+V+TDG+R + + + GG+ P+ + L
Sbjct: 199 GSVYRLLKNWPVRNVKAVVLTDGQRVTGIGDLGVQGMPAAVSKASLFTALGGLDPA-DVL 257
Query: 612 PITI 623
PI I
Sbjct: 258 PICI 261
Score = 43.6 bits (98), Expect = 0.004
Identities = 21/44 (47%), Positives = 28/44 (63%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
+SG++ L+ NKGM+F +ER L + GLLPP V Q QVE
Sbjct: 81 ISGVELLRSGRYNKGMSFARDERDRLNLRGLLPPAVFDQATQVE 124
>UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187;
cellular organisms|Rep: NAD-dependent malic enzyme -
Vibrio vulnificus
Length = 562
Score = 102 bits (245), Expect = 7e-21
Identities = 54/136 (39%), Positives = 73/136 (53%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
++IYL + D NE LFYR V +++ EMMPI+YTPTVG AC+ F +YRR RGLF++ ++
Sbjct: 70 KHIYLRNIQDTNETLFYRLVQNHITEMMPIIYTPTVGAACENFSNIYRRGRGLFVSYANR 129
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
+ D+L N +V+ IVVTDGER GGI P+ L
Sbjct: 130 DRIDDILNNASNHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYT-L 188
Query: 612 PITI*RGYEQPSPCLD 659
PI + G P D
Sbjct: 189 PIVLDVGTNNPQRLAD 204
Score = 42.7 bits (96), Expect = 0.008
Identities = 21/52 (40%), Positives = 30/52 (57%)
Frame = +1
Query: 97 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 252
L P LNKG AF+ EER + GLLP +T +EQVE + +E+ ++
Sbjct: 18 LSTPLLNKGSAFSAEERISFNLEGLLPETTETIQEQVERAYMQYKAFESDMD 69
>UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:
Malic enzyme - Botryotinia fuckeliana B05.10
Length = 685
Score = 100 bits (239), Expect = 4e-20
Identities = 39/85 (45%), Positives = 63/85 (74%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+YL L + N HLFYR V +++ ++ P++YTPTVG AC ++ +Y++P GL+++ HD+
Sbjct: 146 KYMYLSNLRNNNVHLFYRLVQEHLTDITPLIYTPTVGEACLRWSEIYQQPEGLYLSYHDR 205
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G++ +VL NW ++DV VVTDG R
Sbjct: 206 GNLEEVLGNWRQSDVEMTVVTDGSR 230
Score = 43.2 bits (97), Expect = 0.006
Identities = 19/22 (86%), Positives = 20/22 (90%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYT 574
LGLGDLG GMGIPVGKL+LYT
Sbjct: 232 LGLGDLGVNGMGIPVGKLSLYT 253
>UniRef50_A7IMB8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Alphaproteobacteria|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Xanthobacter
sp. (strain Py2)
Length = 550
Score = 97.9 bits (233), Expect = 2e-19
Identities = 43/85 (50%), Positives = 59/85 (69%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
QY YLM L RNE +FY+ V + +PI+Y PTV AC+ FG +YRRPRG++IT H K
Sbjct: 75 QYSYLMDLEARNETVFYKAVMSDPKRFIPILYDPTVADACEAFGNLYRRPRGMYITRHMK 134
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + +VL+NWP+ D+R + V+ G R
Sbjct: 135 GRMAEVLRNWPQKDIRFVCVSTGGR 159
Score = 54.4 bits (125), Expect = 2e-06
Identities = 29/67 (43%), Positives = 41/67 (61%)
Frame = +1
Query: 67 PTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENP 246
P S LSGL+ L P NKG A+T ++R+ LG+ GLLP V+T + QVE +D ++
Sbjct: 13 PKSNLSGLNLLHDPVRNKGTAYTRDDRRQLGLEGLLPHAVETLDRQVERVLDHLDHVKDE 72
Query: 247 LNNTSIL 267
L+ S L
Sbjct: 73 LDQYSYL 79
Score = 45.2 bits (102), Expect = 0.001
Identities = 20/31 (64%), Positives = 23/31 (74%)
Frame = +2
Query: 485 CCNRRRTYLGLGDLGACGMGIPVGKLALYTA 577
C + LGLGD+GA GMGIP+GKL LYTA
Sbjct: 153 CVSTGGRILGLGDIGANGMGIPIGKLQLYTA 183
>UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic
enzyme - Mastigamoeba balamuthi (Phreatamoeba balamuthi)
Length = 568
Score = 97.9 bits (233), Expect = 2e-19
Identities = 45/85 (52%), Positives = 58/85 (68%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+YL L RN+ LF+ V +V E +P+VYTPTVG C KF +R P GL+IT DK
Sbjct: 91 KYLYLSQLSQRNQTLFFYLVQHHVEECVPLVYTPTVGEGCTKFSAEFRNPTGLYITPEDK 150
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GHV ++L+NWP +V IVVTDG R
Sbjct: 151 GHVAEILENWPH-EVEIIVVTDGGR 174
Score = 41.1 bits (92), Expect = 0.024
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG+ GMGIP+GKL LY A
Sbjct: 176 LGLGDLGSNGMGIPIGKLHLYIA 198
Score = 36.3 bits (80), Expect = 0.67
Identities = 24/62 (38%), Positives = 29/62 (46%)
Frame = +1
Query: 64 QPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN 243
+P GL+G L G AFT EER+ L I GLLP V+T E Q +
Sbjct: 30 RPADGLAGPSWLTLAPT--GTAFTTEERKALRIRGLLPHAVETIEAQAARAYAQLTSQPT 87
Query: 244 PL 249
PL
Sbjct: 88 PL 89
>UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila
melanogaster|Rep: Malic enzyme - Drosophila melanogaster
(Fruit fly)
Length = 610
Score = 97.5 bits (232), Expect = 3e-19
Identities = 43/86 (50%), Positives = 60/86 (69%), Gaps = 1/86 (1%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y YL L E L+++FV+ NV ++PI+YTPTVGLAC +G++YR G+ IT HD+
Sbjct: 102 RYRYLRALRQGYERLYFQFVSKNVHAVLPIIYTPTVGLACTVYGMLYRGMTGIHITKHDR 161
Query: 432 GHVYDVLKNWP-ETDVRAIVVTDGER 506
GH+ +L NWP V+AI VTDG+R
Sbjct: 162 GHMKQILSNWPMRRSVKAICVTDGQR 187
Score = 44.8 bits (101), Expect = 0.002
Identities = 20/43 (46%), Positives = 30/43 (69%)
Frame = +1
Query: 115 NKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN 243
NK +AFT+EERQ L IHGL+P V+T +EQ+ + + +E+
Sbjct: 56 NKALAFTLEERQRLCIHGLMPACVRTYDEQMLAIESNFHSFES 98
Score = 44.4 bits (100), Expect = 0.003
Identities = 21/32 (65%), Positives = 23/32 (71%)
Frame = +2
Query: 485 CCNRRRTYLGLGDLGACGMGIPVGKLALYTAL 580
C + LGLGDLGA GMGI VGK+ LYTAL
Sbjct: 181 CVTDGQRILGLGDLGANGMGIAVGKMELYTAL 212
>UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,
partial; n=1; Macaca mulatta|Rep: PREDICTED: similar to
Y48B6A.12, partial - Macaca mulatta
Length = 456
Score = 95.9 bits (228), Expect = 8e-19
Identities = 44/85 (51%), Positives = 59/85 (69%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YIYL L DRNE L+++ + D+VAEMMPIVYTP VG ACQ FG ++R RGL+ + +K
Sbjct: 63 KYIYLESLHDRNETLYFKLLVDHVAEMMPIVYTPVVGKACQLFGHIFRNARGLYFNLSEK 122
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G+ +++ N D IVVTDG R
Sbjct: 123 GNFKEMVWNSNVRDADIIVVTDGSR 147
Score = 42.3 bits (95), Expect = 0.010
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIP+GKL+LY A
Sbjct: 149 LGLGDLGTNGMGIPIGKLSLYVA 171
>UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus
californicus|Rep: Malic enzyme - Tigriopus californicus
(Marine copepod)
Length = 322
Score = 95.9 bits (228), Expect = 8e-19
Identities = 40/81 (49%), Positives = 55/81 (67%)
Frame = +3
Query: 264 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 443
L + DRNE LFYR + DN +M PI+YTPTVG AC F +YRRPRG++ + D+G +
Sbjct: 124 LQSVQDRNETLFYRILMDNFQDMAPIIYTPTVGWACSHFSQLYRRPRGMYFSHGDRGEMA 183
Query: 444 DVLKNWPETDVRAIVVTDGER 506
++ NW +V A+V+TDG R
Sbjct: 184 SMVYNWESDEVDAVVITDGSR 204
Score = 39.9 bits (89), Expect = 0.055
Identities = 17/40 (42%), Positives = 26/40 (65%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQ 204
G + + P NKG+AF + ER L + GL+PPR+ + +EQ
Sbjct: 45 GRNLVSDPISNKGLAFPLSERDRLSVRGLVPPRILSIQEQ 84
Score = 36.3 bits (80), Expect = 0.67
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GI +GKL LY A
Sbjct: 206 LGLGDLGLGGLGISIGKLDLYVA 228
>UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isoform,
mitochondrial precursor; n=41; Eukaryota|Rep:
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor - Solanum tuberosum (Potato)
Length = 626
Score = 95.9 bits (228), Expect = 8e-19
Identities = 40/78 (51%), Positives = 55/78 (70%)
Frame = +3
Query: 273 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 452
L DRNE L+Y+ + +N+ E PIVYTPTVGL CQK+ ++RRPRG++ + D+G + ++
Sbjct: 122 LHDRNETLYYKVLMENIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMV 181
Query: 453 KNWPETDVRAIVVTDGER 506
NWP V IVVTDG R
Sbjct: 182 YNWPADQVDMIVVTDGSR 199
Score = 44.8 bits (101), Expect = 0.002
Identities = 23/51 (45%), Positives = 28/51 (54%)
Frame = +1
Query: 88 LDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYE 240
LD L P NKG AF+ ER L I GLLPP V + E+Q+ + R E
Sbjct: 50 LDILHDPWFNKGTAFSFTERDRLHIRGLLPPNVMSFEQQIARFMADLKRLE 100
Score = 35.9 bits (79), Expect = 0.89
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GI +GKL LY A
Sbjct: 201 LGLGDLGIQGIGIAIGKLDLYVA 223
>UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;
Pezizomycotina|Rep: NADP-dependent malic enzyme MaeA -
Aspergillus fumigatus (Sartorya fumigata)
Length = 661
Score = 94.3 bits (224), Expect = 2e-18
Identities = 36/85 (42%), Positives = 59/85 (69%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+++YL L N HLFYR V D++ E+ P++YTP VG ACQK+ +Y++P G++++ D+
Sbjct: 138 KFLYLSTLRKNNVHLFYRLVTDHLKELTPLIYTPVVGEACQKWSEIYQQPEGMYLSWEDR 197
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G++ V+ NWP+ +V +TDG R
Sbjct: 198 GNLAAVIANWPQPNVEITCITDGSR 222
Score = 45.6 bits (103), Expect = 0.001
Identities = 21/31 (67%), Positives = 22/31 (70%)
Frame = +2
Query: 485 CCNRRRTYLGLGDLGACGMGIPVGKLALYTA 577
C LGLGDLG GMGIP+GKLALYTA
Sbjct: 216 CITDGSRILGLGDLGINGMGIPIGKLALYTA 246
>UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;
n=1; Neocallimastix frontalis|Rep: Malic enzyme,
hydrogenosomal precursor - Neocallimastix frontalis
(Rumen fungus)
Length = 592
Score = 93.9 bits (223), Expect = 3e-18
Identities = 40/85 (47%), Positives = 57/85 (67%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
++IYL L +RNE L+Y+ + +N E+ PI+YTP VG ACQKF ++ + RG++ + D+
Sbjct: 103 KFIYLNHLQNRNETLYYKMILENFVELAPIIYTPVVGEACQKFHKIFTQTRGMYFSTADR 162
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + V NWP DV IVVTDG R
Sbjct: 163 GQMSAVAANWPYDDVDVIVVTDGSR 187
Score = 63.3 bits (147), Expect = 5e-09
Identities = 28/56 (50%), Positives = 39/56 (69%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
+GLD L P LNKG AFT +E+ LGI GL+PPR ++ E Q + CK ++D+ +PL
Sbjct: 46 TGLDILNDPKLNKGSAFTADEKDRLGIRGLVPPRPQSLEAQYKRCKTNLDKISDPL 101
Score = 38.7 bits (86), Expect = 0.13
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALY 571
LGLGDLGA GM IP+GKL LY
Sbjct: 189 LGLGDLGAGGMQIPIGKLTLY 209
>UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 603
Score = 93.1 bits (221), Expect = 6e-18
Identities = 39/85 (45%), Positives = 59/85 (69%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
++ YL + R+ L+YRF+ +N+ + +PIVYTPTVG +GL +++ LFI+IHDK
Sbjct: 94 RFTYLSAVHHRHRRLYYRFIKENIEKSLPIVYTPTVGDVVATYGLNFQQAISLFISIHDK 153
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
GH+ D++ NW + V+AI VTDG R
Sbjct: 154 GHIRDLMHNWVDEGVKAICVTDGGR 178
Score = 42.7 bits (96), Expect = 0.008
Identities = 18/31 (58%), Positives = 26/31 (83%)
Frame = +1
Query: 115 NKGMAFTIEERQLLGIHGLLPPRVKTQEEQV 207
NKG+AFTI+ERQ LGI GL+P V++ ++Q+
Sbjct: 48 NKGLAFTIKERQRLGIMGLMPCSVRSMDDQM 78
Score = 38.7 bits (86), Expect = 0.13
Identities = 17/24 (70%), Positives = 20/24 (83%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTAL 580
LGLGD+GA MGI +GK+ LYTAL
Sbjct: 180 LGLGDMGANAMGISLGKMILYTAL 203
>UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP+-dependent malic enzyme -
Ostreococcus tauri
Length = 580
Score = 91.9 bits (218), Expect = 1e-17
Identities = 39/85 (45%), Positives = 58/85 (68%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y L+ L +E FYR + ++PI+YTPTVG AC KFG + +RP GL+++ +D
Sbjct: 102 KYKQLVALQMTDESTFYRMLRSQTETLLPILYTPTVGEACVKFGTLVQRPMGLWVSSNDA 161
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G+V +++NWP TDV+ V+TDGER
Sbjct: 162 GNVKQLIRNWPATDVKIAVITDGER 186
Score = 32.7 bits (71), Expect = 8.3
Identities = 15/23 (65%), Positives = 16/23 (69%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGD GA GMGI GK +Y A
Sbjct: 188 LGLGDQGANGMGISAGKSMVYAA 210
>UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 635
Score = 89.0 bits (211), Expect = 9e-17
Identities = 39/80 (48%), Positives = 54/80 (67%), Gaps = 2/80 (2%)
Frame = +3
Query: 273 LLDRNEHLFYR--FVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYD 446
L DRNE ++Y+ + DN+ E PIVYTPTVGL CQ + ++RRPRG++ + D+G +
Sbjct: 154 LHDRNETMYYKAEVLIDNIEEHAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMS 213
Query: 447 VLKNWPETDVRAIVVTDGER 506
++ NWP V IVVTDG R
Sbjct: 214 MVYNWPADQVDMIVVTDGSR 233
Score = 44.0 bits (99), Expect = 0.003
Identities = 18/42 (42%), Positives = 27/42 (64%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
G D L P N+G F++ ER LG+ GLLPP V + ++Q++
Sbjct: 45 GSDILHDPWFNRGTGFSMTERDRLGLRGLLPPNVVSSQQQID 86
Score = 36.3 bits (80), Expect = 0.67
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GI +GKL LY A
Sbjct: 235 LGLGDLGVHGIGIAIGKLDLYVA 257
>UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus tauri
Length = 639
Score = 88.6 bits (210), Expect = 1e-16
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = +3
Query: 261 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 440
+L L DRNE LFYR V D++ E+ PI+YTPTVG AC F + RR RG++ ++ D+G +
Sbjct: 140 HLQDLKDRNETLFYRLVHDHIEELAPIIYTPTVGDACLNFSKLLRRARGMYFSVDDRGDI 199
Query: 441 YDVLKNWPETDVRAIVVTDGER 506
++ NW + V IVVTDG R
Sbjct: 200 NSMMFNW-KRSVSVIVVTDGSR 220
Score = 42.3 bits (95), Expect = 0.010
Identities = 21/56 (37%), Positives = 30/56 (53%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 252
G++ L +P NKG +FT ER+ LG+ GL+PPR +Q E PL+
Sbjct: 81 GIEVLHNPVYNKGTSFTASERERLGVRGLVPPRFFPIGQQATKIWAQNQSLERPLD 136
Score = 34.7 bits (76), Expect = 2.1
Identities = 16/23 (69%), Positives = 17/23 (73%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGI GK+ LY A
Sbjct: 222 LGLGDLGTNGMGISQGKVDLYVA 244
>UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor
circinelloides|Rep: Malic enzyme - Mucor circinelloides
Length = 617
Score = 87.8 bits (208), Expect = 2e-16
Identities = 37/85 (43%), Positives = 57/85 (67%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
++++L L DRNE L+Y+ + +++ E+ I+YTPTVGLA Q +YRR RG++ + D+
Sbjct: 106 KFVFLAALHDRNETLYYKIIMEHLEELAGIIYTPTVGLASQMSHSIYRRSRGMYFSSQDR 165
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + ++ NWP V IVVTDG R
Sbjct: 166 GQMSAMVYNWPHDKVDVIVVTDGSR 190
Score = 50.0 bits (114), Expect = 5e-05
Identities = 24/55 (43%), Positives = 36/55 (65%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
G++ L P L+KG AF+I ER+ L I GL+PPR + ++Q+ K ++D E PL
Sbjct: 50 GVNLLHDPLLSKGTAFSIAERERLSIRGLVPPRCQEMDKQLLRIKRNLDACETPL 104
Score = 40.7 bits (91), Expect = 0.031
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLGA GM IP+GKL+LY A
Sbjct: 192 LGLGDLGANGMEIPIGKLSLYVA 214
>UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:
Malic enzyme - Ustilago maydis (Smut fungus)
Length = 634
Score = 87.4 bits (207), Expect = 3e-16
Identities = 38/87 (43%), Positives = 55/87 (63%), Gaps = 2/87 (2%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+++ L L N L+Y + N E++P++YTPTVG ACQKF +YRRP GL I++ DK
Sbjct: 133 KHVMLASLRQTNTRLYYATILANKEEILPLIYTPTVGEACQKFSHIYRRPEGLSISLEDK 192
Query: 432 GHVYDVLKNW--PETDVRAIVVTDGER 506
G + +++NW P R V+TDG R
Sbjct: 193 GKIASIVENWPVPAGSPRIAVITDGSR 219
Score = 35.9 bits (79), Expect = 0.89
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G GI +GKL+LY A
Sbjct: 221 LGLGDLGWNGQGISIGKLSLYVA 243
>UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 1023
Score = 87.0 bits (206), Expect = 4e-16
Identities = 36/85 (42%), Positives = 53/85 (62%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+YL + +N LFYR + D+ EMMP+VYTPT+G C ++ +Y RP L+I+I +
Sbjct: 528 RYLYLSTIKSQNVDLFYRLLMDHAKEMMPLVYTPTIGDVCLQYSTLYTRPEALYISIKQR 587
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
+ +L+NWP VVTDG R
Sbjct: 588 KSIRTILRNWPYPQPEICVVTDGSR 612
Score = 44.0 bits (99), Expect = 0.003
Identities = 19/23 (82%), Positives = 21/23 (91%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GIP+GKLALYTA
Sbjct: 614 LGLGDLGVNGVGIPIGKLALYTA 636
>UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10;
Francisella tularensis|Rep: NAD-dependent malic enzyme -
Francisella tularensis subsp. novicida (strain U112)
Length = 604
Score = 85.4 bits (202), Expect = 1e-15
Identities = 38/84 (45%), Positives = 56/84 (66%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y++L L D N LFY FV +N+ E+MPI+YTPTVG A QK+ +R+ GLFI+I K
Sbjct: 83 KYVFLNRLHDLNTTLFYHFVRENLEEIMPIIYTPTVGEAVQKYSSSFRKQSGLFISISHK 142
Query: 432 GHVYDVLKNWPETDVRAIVVTDGE 503
H+ +L+ + + ++VTDGE
Sbjct: 143 KHIARILERYEYNSIDLVLVTDGE 166
Score = 36.7 bits (81), Expect = 0.51
Identities = 21/59 (35%), Positives = 32/59 (54%)
Frame = +1
Query: 73 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
+ L+G L + LNK +AF+ EER + G LP +V++ EEQ + +D N L
Sbjct: 23 TNLTGRQLLNNRVLNKDVAFSQEERIAFDLIGYLPEKVESLEEQAIRVRRQLDLKPNSL 81
>UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 549
Score = 85.0 bits (201), Expect = 1e-15
Identities = 39/85 (45%), Positives = 55/85 (64%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI+L L +NE LFYR + +++ ++MPIVYTPTVG AC F +YR G++ + D
Sbjct: 65 RYIFLENLHMQNERLFYRVLVEHLEDLMPIVYTPTVGEACINFDALYRNRCGMYFSRLDS 124
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
G + +L NWP + IVVTDG R
Sbjct: 125 GVMRRMLDNWPSPETEIIVVTDGGR 149
Score = 44.8 bits (101), Expect = 0.002
Identities = 21/52 (40%), Positives = 30/52 (57%)
Frame = +1
Query: 97 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 252
L P LNKG FT +R+ LG+ GLLP + +T E QV+ + +E +N
Sbjct: 13 LDSPSLNKGTGFTFTQRERLGLRGLLPRKYETVEIQVKRAWTQLCAFEEDMN 64
Score = 35.9 bits (79), Expect = 0.89
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GM I VGK++LY A
Sbjct: 151 LGLGDLGTNGMAISVGKVSLYVA 173
>UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6;
Deinococci|Rep: Malate oxidoreductase - Deinococcus
radiodurans
Length = 580
Score = 83.8 bits (198), Expect = 3e-15
Identities = 41/82 (50%), Positives = 52/82 (63%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+++YL L DRNE LFY ++ +V EM+PIVYTPTVG A +KF +YR PRGL ++
Sbjct: 93 KHVYLRNLQDRNEVLFYALLSHHVEEMLPIVYTPTVGDAVKKFSQIYRYPRGLTLSTRTI 152
Query: 432 GHVYDVLKNWPETDVRAIVVTD 497
L N P DVR IV TD
Sbjct: 153 ERAEQALANVPLNDVRIIVATD 174
Score = 40.7 bits (91), Expect = 0.031
Identities = 20/42 (47%), Positives = 27/42 (64%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
G + + P LNKG AFT EER+ G+ GLL P+V + E +E
Sbjct: 37 GFNLTRIPLLNKGTAFTAEEREAHGLDGLLAPQVDSLEVLIE 78
Score = 33.1 bits (72), Expect = 6.3
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYT 574
LG+GD G GM I +GKL+LYT
Sbjct: 179 LGIGDQGFGGMAISIGKLSLYT 200
>UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep:
Malic enzyme - Trichomonas vaginalis G3
Length = 567
Score = 82.6 bits (195), Expect = 8e-15
Identities = 39/86 (45%), Positives = 56/86 (65%), Gaps = 1/86 (1%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAE-MMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD 428
+YI+L ++N F+RF+ + E MPI+YTPTVG ACQK+ + RG++IT D
Sbjct: 80 KYIFLANEREKNSQSFWRFLFTHPPEETMPILYTPTVGEACQKWATHRQSYRGIYITPED 139
Query: 429 KGHVYDVLKNWPETDVRAIVVTDGER 506
G + D+L+N+P D+R IVVTD R
Sbjct: 140 SGKIKDILRNYPRQDIRCIVVTDAGR 165
Score = 44.0 bits (99), Expect = 0.003
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTAL 580
LGLGDLGA G+GIPVGKL LYT +
Sbjct: 167 LGLGDLGASGLGIPVGKLMLYTLI 190
Score = 43.2 bits (97), Expect = 0.006
Identities = 22/56 (39%), Positives = 31/56 (55%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
+G+ L+ LNKG AFT EER + GLLP +V T++EQ + + PL
Sbjct: 23 TGMTLLQDGDLNKGTAFTKEERDRFNLRGLLPYKVFTKDEQAARIRRQFELMPTPL 78
>UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=4;
Gammaproteobacteria|Rep: Malic enzyme aka malate
dehydrogenase (Oxaloacetate-decarboxylating) (NADP(+)) -
Psychromonas ingrahamii (strain 37)
Length = 571
Score = 80.2 bits (189), Expect = 4e-14
Identities = 37/84 (44%), Positives = 54/84 (64%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y YL L DRNE LFY ++ N+ EM PI+YTPTVG ACQ+F ++ RGL++T +
Sbjct: 80 KYQYLRALQDRNETLFYALISRNIEEMTPIIYTPTVGKACQEFSHRFQIARGLYLTTDNI 139
Query: 432 GHVYDVLKNWPETDVRAIVVTDGE 503
V + + + D++ IVVTD +
Sbjct: 140 HDVGSMAREFTGKDIQIIVVTDSQ 163
Score = 46.4 bits (105), Expect = 6e-04
Identities = 25/58 (43%), Positives = 38/58 (65%), Gaps = 3/58 (5%)
Frame = +1
Query: 46 WGSGDG---QPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
W +G +P S L+G + L + LNK AFT +ER+ ++GLLPPRV+T E+Q++
Sbjct: 9 WEDSEGNVFRPVS-LTGNELLNNRTLNKSTAFTYQEREDFDLNGLLPPRVQTFEDQLK 65
Score = 39.5 bits (88), Expect = 0.072
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYT 574
LG+GD G GMGIP+GKL+LYT
Sbjct: 166 LGIGDQGVGGMGIPIGKLSLYT 187
>UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2;
n=37; Bacteria|Rep: Probable NAD-dependent malic enzyme
2 - Bacillus subtilis
Length = 582
Score = 75.8 bits (178), Expect = 9e-13
Identities = 36/86 (41%), Positives = 56/86 (65%), Gaps = 2/86 (2%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
Q +YL L +RNE LFY+ + +++ EM+P+VYTPTVG A Q++ YRRP+G++++I +
Sbjct: 91 QNVYLSDLANRNEVLFYKLLKNHLREMLPVVYTPTVGEAIQEYSHEYRRPQGIYLSIDNI 150
Query: 432 GHVYDVLKNWPET--DVRAIVVTDGE 503
+ +N T D+ IV TD E
Sbjct: 151 DGIEKAFENLHATAGDIDLIVATDSE 176
Score = 51.6 bits (118), Expect = 2e-05
Identities = 24/51 (47%), Positives = 35/51 (68%)
Frame = +1
Query: 58 DGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
+G + L G + L P LNKG+AF++EERQ LG+ GLLPP V + ++Q +
Sbjct: 26 EGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGLEGLLPPTVLSLDQQAQ 76
Score = 33.9 bits (74), Expect = 3.6
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LG+GD G G+ I +GKLA+YTA
Sbjct: 179 LGIGDWGVGGINIAIGKLAVYTA 201
>UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20;
Trypanosomatidae|Rep: Malic enzyme, putative -
Leishmania major
Length = 573
Score = 74.5 bits (175), Expect = 2e-12
Identities = 39/124 (31%), Positives = 65/124 (52%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y L + + N L+Y + + + +PIVYTPTVG ACQ++G +Y++ GL++ + K
Sbjct: 75 RYQLLRNVQNTNVTLYYAILTRYLKQTLPIVYTPTVGEACQRYGDLYQKDHGLYLDVASK 134
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G V +++N +T++ IV+TDG R GG+KPS L
Sbjct: 135 GKVRRLIQNLRKTNIDVIVITDGSRILGLGDLGSNGIGISIGKCSLYVAAGGVKPS-RVL 193
Query: 612 PITI 623
P+ +
Sbjct: 194 PVVM 197
Score = 56.0 bits (129), Expect = 8e-07
Identities = 25/61 (40%), Positives = 39/61 (63%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNTSI 264
G+D+L++ NKG AFT ER + + GLLPP V+T ++QVE ++R+ P+N +
Sbjct: 19 GVDYLRNRFTNKGTAFTAAERSHMNVEGLLPPSVETLDDQVERYWDQLNRFNEPINRYQL 78
Query: 265 L 267
L
Sbjct: 79 L 79
>UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 611
Score = 74.1 bits (174), Expect = 3e-12
Identities = 37/83 (44%), Positives = 53/83 (63%), Gaps = 2/83 (2%)
Frame = +3
Query: 261 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 440
++ + ++NE LFYR + D++ EM +VYTPT G A Q + ++RRP G+F+ I+D V
Sbjct: 94 FMTSMKEQNEVLFYRLLHDHLDEMFSVVYTPTEGEAIQNYSRLFRRPEGVFLNINDMDSV 153
Query: 441 YDVLKNW--PETDVRAIVVTDGE 503
L W PE D+ IVVTDGE
Sbjct: 154 KRDLAQWGKPE-DIDYIVVTDGE 175
Score = 52.8 bits (121), Expect = 7e-06
Identities = 25/53 (47%), Positives = 33/53 (62%)
Frame = +1
Query: 52 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
S G L G L HP NKG AFT EER+ +HGLLPP++++ E+QV+
Sbjct: 24 STSGPLECALKGTVLLNHPYFNKGSAFTKEERRDFALHGLLPPQIQSLEQQVQ 76
>UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 600
Score = 72.5 bits (170), Expect = 8e-12
Identities = 33/88 (37%), Positives = 54/88 (61%), Gaps = 3/88 (3%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
++++L + + +LF+ + D + E+ PIVYTPTVG ACQK+ +Y P GL++ I DK
Sbjct: 107 KHVHLSKIRREDPNLFFSVMRDELTELAPIVYTPTVGEACQKYSQIYSGPEGLYLNIEDK 166
Query: 432 GHVYDVLKNWPETDV---RAIVVTDGER 506
+ ++L + V + +VVTDG R
Sbjct: 167 DRIPEILHQYASKLVAPPQILVVTDGSR 194
Score = 37.9 bits (84), Expect = 0.22
Identities = 18/23 (78%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGI VGKL LY A
Sbjct: 196 LGLGDLGIGGMGISVGKLNLYVA 218
>UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2;
Aeromonas|Rep: NAD-dependent malic enzyme - Aeromonas
salmonicida (strain A449)
Length = 516
Score = 69.3 bits (162), Expect = 8e-11
Identities = 34/85 (40%), Positives = 53/85 (62%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
Q++ L L + N LFY V ++ E++PI+YTP VG ACQ+ +Y R GL+++ HD+
Sbjct: 51 QHLLLRQLQEDNPVLFYDLVRHHLPELLPIIYTPVVGEACQRHSDLYLRSHGLYLSWHDR 110
Query: 432 GHVYDVLKNWPETDVRAIVVTDGER 506
+ D + E +V IV++DGER
Sbjct: 111 DDL-DAIFAAVEQEVDVIVISDGER 134
Score = 39.5 bits (88), Expect = 0.072
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGI +GKLALY+A
Sbjct: 136 LGLGDLGIGGMGICIGKLALYSA 158
>UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia
intestinalis|Rep: Malic enzyme - Giardia lamblia
(Giardia intestinalis)
Length = 557
Score = 68.5 bits (160), Expect = 1e-10
Identities = 36/88 (40%), Positives = 51/88 (57%), Gaps = 1/88 (1%)
Frame = +3
Query: 246 T*QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYR-RPRGLFITI 422
T +++YL L + NE LF F + E++PIVYTPTVG AC + L+++ PRG ++
Sbjct: 59 TEKWLYLTRLQEVNETLFSGFCLKYLKEVLPIVYTPTVGTACSNYSLLWQGYPRGFYLNR 118
Query: 423 HDKGHVYDVLKNWPETDVRAIVVTDGER 506
G V + WP + R IV TDG R
Sbjct: 119 THLGKVKQIFDQWPYSP-RIIVATDGTR 145
Score = 37.9 bits (84), Expect = 0.22
Identities = 19/50 (38%), Positives = 26/50 (52%)
Frame = +1
Query: 97 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENP 246
L++ NK AFT ER+ I LP RV+T E+Q+ C+ D P
Sbjct: 9 LRNKDCNKDTAFTAAEREAHHIVARLPARVETIEQQISRCRAQFDVLTTP 58
>UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme;
n=1; Desulfotalea psychrophila|Rep: Related to
NAD-dependent malic enzyme - Desulfotalea psychrophila
Length = 578
Score = 68.1 bits (159), Expect = 2e-10
Identities = 30/84 (35%), Positives = 49/84 (58%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+YI++ L DRN L + + ++ + M I+YTPTVGLA QK+ ++R+ GL +
Sbjct: 81 KYIFIRSLFDRNVTLAHALIQSDLEKFMGIIYTPTVGLAVQKYSAMFRQANGLHFSPDTI 140
Query: 432 GHVYDVLKNWPETDVRAIVVTDGE 503
D+L+ + D+R VVTD +
Sbjct: 141 DQAEDILRRFAHRDIRVAVVTDNQ 164
Score = 37.1 bits (82), Expect = 0.39
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +1
Query: 115 NKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN 243
NK AF+ +ER G+ G LPP + E QVE C + + E+
Sbjct: 35 NKSTAFSSKERDDFGLQGSLPPGFRDLEAQVENCHIKLGEKES 77
>UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2;
Mucoromycotina|Rep: Malic enzyme protein 2 - Mortierella
alpina (Mortierella renispora)
Length = 669
Score = 68.1 bits (159), Expect = 2e-10
Identities = 34/97 (35%), Positives = 56/97 (57%), Gaps = 12/97 (12%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVY------RRPRGLF 413
+Y++L L + N LFY V D + E +P++YTPTVG ACQ + +Y +P GLF
Sbjct: 153 KYVFLAWLRNTNVRLFYGLVGDQLEETLPLIYTPTVGTACQNYSSIYPFLAPPGQPDGLF 212
Query: 414 ITIHDKGHVYDVLKNW------PETDVRAIVVTDGER 506
++I+D ++ +++N+ P + V+TDG R
Sbjct: 213 LSINDLPNLTQIIQNYKPFPQDPSLTPQIAVITDGSR 249
Score = 41.9 bits (94), Expect = 0.014
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG GMGIPVGKL LY A
Sbjct: 251 LGLGDLGVGGMGIPVGKLQLYVA 273
>UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|Rep:
Malic enzyme - Aspergillus niger
Length = 609
Score = 68.1 bits (159), Expect = 2e-10
Identities = 40/135 (29%), Positives = 66/135 (48%), Gaps = 1/135 (0%)
Frame = +3
Query: 261 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 440
++ + +NE L+Y+ + ++ EM+ I+YTPT G A Q + ++R+P G F+ I D+ +
Sbjct: 116 FMASMKAQNEVLYYKLIDTHLKEMLSIIYTPTEGDAIQNYSRLFRKPEGCFLNIRDQDRI 175
Query: 441 YDVLKNWPE-TDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGLPI 617
+ L N+ +V IVV+DGE A T GI PS LP+
Sbjct: 176 EECLSNFSRGEEVDYIVVSDGEEILGIGDQGVGAILISVAKLALTTLCAGIHPS-RQLPV 234
Query: 618 TI*RGYEQPSPCLDQ 662
+ G + S D+
Sbjct: 235 VLDCGTDNESLLTDE 249
Score = 50.4 bits (115), Expect = 4e-05
Identities = 25/61 (40%), Positives = 34/61 (55%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNTSI 264
G D L+ NKG AFT EER+ +HGLLPP ++T EEQV+ +N L +
Sbjct: 57 GRDALQSCQFNKGSAFTEEERKTFKLHGLLPPNIQTLEEQVQRAYEQYSSRDNDLAKNTF 116
Query: 265 L 267
+
Sbjct: 117 M 117
>UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococcus
sp. WH 5701|Rep: Malate oxidoreductase - Synechococcus
sp. WH 5701
Length = 517
Score = 67.7 bits (158), Expect = 2e-10
Identities = 35/91 (38%), Positives = 52/91 (57%), Gaps = 1/91 (1%)
Frame = +3
Query: 234 IRKST*QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPR-GL 410
+R ++ + + L N LF+RF+AD++ +MPIVYTPTVG A Q+F L YR P G+
Sbjct: 18 LRNDLERFRFAVALRQANLTLFHRFLADHIEAVMPIVYTPTVGAAIQRFSLDYRTPSGGV 77
Query: 411 FITIHDKGHVYDVLKNWPETDVRAIVVTDGE 503
F+ D + VL V I++TD +
Sbjct: 78 FLAAPDLERIESVLSQAATGPVDLILITDSQ 108
>UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3;
Schizosaccharomyces pombe|Rep: NAD-dependent malic
enzyme - Schizosaccharomyces pombe (Fission yeast)
Length = 565
Score = 62.5 bits (145), Expect = 9e-09
Identities = 31/85 (36%), Positives = 52/85 (61%), Gaps = 3/85 (3%)
Frame = +3
Query: 258 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI--HDK 431
+YL L N+ LFY ++ ++ EM+PI+YTPT G A ++F +YR P G ++ I +D
Sbjct: 74 LYLSQLSVTNQTLFYALISQHLIEMIPIIYTPTEGDAIKQFSDIYRYPEGCYLDIDHNDL 133
Query: 432 GHVYDVLKNWPETD-VRAIVVTDGE 503
++ L + ++D V I++TD E
Sbjct: 134 SYIKQQLSEFGKSDSVEYIIITDSE 158
Score = 43.2 bits (97), Expect = 0.006
Identities = 22/46 (47%), Positives = 27/46 (58%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELC 216
L G+ L P NK AFT EERQ I LPP V+T ++QV+ C
Sbjct: 13 LKGVTLLNSPRYNKDTAFTPEERQKFEISSRLPPIVETLQQQVDRC 58
>UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 633
Score = 61.3 bits (142), Expect = 2e-08
Identities = 35/116 (30%), Positives = 52/116 (44%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
QYIYL L RN LFY + N +P+ +++ +GL+I I D
Sbjct: 128 QYIYLTYLSRRNRRLFYYLLLSNPDRFVPMTDASGSIDLLMVHRMIHSMGQGLYICIKDL 187
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPS 599
GHV +L NWP VR ++V++G L++N +GGI P+
Sbjct: 188 GHVSQILSNWPFRCVRCLLVSNGASVLSVGDLGVDEMPILFSNLHQNVVYGGIHPA 243
Score = 57.6 bits (133), Expect = 3e-07
Identities = 26/57 (45%), Positives = 37/57 (64%)
Frame = +1
Query: 73 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN 243
S + GL L NKG+AFT+ ER++L IHGLLP V+T +EQ E+C ++ + N
Sbjct: 68 SKVDGLWMLNQSNYNKGLAFTLNERRVLSIHGLLPVAVRTIDEQAEICSNLLESFTN 124
>UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep:
Malolactic enzyme - Oenococcus oeni (Leuconostoc oenos)
Length = 541
Score = 56.0 bits (129), Expect = 8e-07
Identities = 27/84 (32%), Positives = 48/84 (57%), Gaps = 2/84 (2%)
Frame = +3
Query: 258 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG-LFITIHDKG 434
++LM + + N LFY+ + +V E MPIVY PT+ + + ++ P+G F+ I+
Sbjct: 62 LFLMEIFNTNHVLFYKLFSQHVVEFMPIVYDPTIADTIENYSELFVEPQGAAFLDINHPE 121
Query: 435 HVYDVLKNWPE-TDVRAIVVTDGE 503
++ LKN D++ +VV+D E
Sbjct: 122 NIQSTLKNAANGRDIKLLVVSDAE 145
Score = 44.8 bits (101), Expect = 0.002
Identities = 20/38 (52%), Positives = 28/38 (73%)
Frame = +1
Query: 97 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
L P +NKG AFT ER+ LG++GLLP +V+ +EQV+
Sbjct: 8 LNDPFINKGTAFTEAEREELGLNGLLPAKVQALQEQVD 45
>UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 539
Score = 55.6 bits (128), Expect = 1e-06
Identities = 26/63 (41%), Positives = 36/63 (57%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+YL L + FYR + E+MP VYTPTVG AC+K+ + G++IT D
Sbjct: 20 RYVYLRELQRASAETFYRALVREPLELMPFVYTPTVGEACEKYHRLGIETNGVYITADDA 79
Query: 432 GHV 440
G V
Sbjct: 80 GRV 82
Score = 37.5 bits (83), Expect = 0.29
Identities = 17/22 (77%), Positives = 18/22 (81%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYT 574
LGLGDLGA GMGI GK+ LYT
Sbjct: 136 LGLGDLGAGGMGISEGKILLYT 157
>UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:
Malic enzyme - Cryptosporidium parvum Iowa II
Length = 614
Score = 54.8 bits (126), Expect = 2e-06
Identities = 29/89 (32%), Positives = 48/89 (53%), Gaps = 4/89 (4%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGL--VYRR--PRGLFIT 419
+Y +L + + LF+ + ++ P+VYTPTVG C +F R GL++
Sbjct: 122 KYTFLENIRSSSFILFHSLLDKYFKDLTPLVYTPTVGEGCIEFSRNPTIRNWLGSGLYLN 181
Query: 420 IHDKGHVYDVLKNWPETDVRAIVVTDGER 506
KG +Y++LK++ D+ IV+TDG R
Sbjct: 182 KSHKGRIYEILKDFKSDDIEIIVLTDGGR 210
Score = 52.0 bits (119), Expect = 1e-05
Identities = 23/63 (36%), Positives = 42/63 (66%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNT 258
L G++ L++P NKG++FT+EER+ G+ GLLP + +T +EQV +I++ ++ +
Sbjct: 64 LKGIELLRNPFYNKGLSFTMEERKEYGLEGLLPAKYETIDEQVSRLWTAINKIDSNIGKY 123
Query: 259 SIL 267
+ L
Sbjct: 124 TFL 126
Score = 40.7 bits (91), Expect = 0.031
Identities = 18/24 (75%), Positives = 20/24 (83%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTAL 580
LGLGDLG GMGIP+GKL+LY L
Sbjct: 212 LGLGDLGLNGMGIPMGKLSLYITL 235
>UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep:
Lmo1915 protein - Listeria monocytogenes
Length = 547
Score = 54.4 bits (125), Expect = 2e-06
Identities = 26/62 (41%), Positives = 38/62 (61%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNTS 261
SG D++ +P LNKG AF+ EER + GLLPP ++T E+Q + I+ E PL+
Sbjct: 5 SGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPLHKHR 64
Query: 262 IL 267
+L
Sbjct: 65 LL 66
Score = 50.0 bits (114), Expect = 5e-05
Identities = 28/83 (33%), Positives = 44/83 (53%), Gaps = 3/83 (3%)
Frame = +3
Query: 264 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRP-RGLFITIHDKGHV 440
L L + N L+Y V NV + +PI+YTPT+G A ++ Y P LF+ +
Sbjct: 66 LTNLYNENRTLYYYVVTKNVTDYLPIIYTPTIGDAVIQYHKDYTAPDEALFVDAFAPEKL 125
Query: 441 YDVLKNWPET--DVRAIVVTDGE 503
+KN+ + ++ IV+TDGE
Sbjct: 126 SASIKNYAKNNPNIDMIVITDGE 148
>UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium
globosum|Rep: Malic enzyme - Chaetomium globosum (Soil
fungus)
Length = 586
Score = 53.2 bits (122), Expect = 5e-06
Identities = 33/98 (33%), Positives = 53/98 (54%), Gaps = 3/98 (3%)
Frame = +3
Query: 210 TMQALDR*IRKST*QYIYLMGLLDRNEHLFYRFVADNVA--EMMPIVYTPTVGLACQKFG 383
++Q LD+ ++++ QY L +N L + V +M +VYTPT G A + F
Sbjct: 65 SIQTLDQQVQRAYEQYSARPDDLAKNTFLTSMKEQNEVLYFKMFSVVYTPTEGDAIENFS 124
Query: 384 LVYRRPRGLFITIHDKGHVYDVLKNW-PETDVRAIVVT 494
++RRP+G+F+ +HD V+ L W D+ IVVT
Sbjct: 125 RLFRRPQGVFLNVHDCDRVHHDLSLWGMPDDIDYIVVT 162
Score = 41.5 bits (93), Expect = 0.018
Identities = 22/53 (41%), Positives = 29/53 (54%)
Frame = +1
Query: 52 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 210
S G L G L H NKG AFT EER+ + GLLP ++T ++QV+
Sbjct: 22 STSGPLECALKGTVLLNHSYFNKGSAFTKEERRDFELSGLLPQSIQTLDQQVQ 74
>UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n=2;
Filobasidiella neoformans|Rep: Nad-dependent malic
enzyme, putative - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 584
Score = 51.2 bits (117), Expect = 2e-05
Identities = 29/82 (35%), Positives = 44/82 (53%), Gaps = 1/82 (1%)
Frame = +3
Query: 261 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT-IHDKGH 437
+L + +N LFY + ++ EM PIVYTPT A + ++RR GL++T +K
Sbjct: 87 FLQSMKAQNWTLFYALLQAHLVEMFPIVYTPTEADAIADYSHLFRRSEGLYLTPPGEKNM 146
Query: 438 VYDVLKNWPETDVRAIVVTDGE 503
D L ++ IVV+DGE
Sbjct: 147 EEDFLDACEGRELELIVVSDGE 168
Score = 34.7 bits (76), Expect = 2.1
Identities = 23/61 (37%), Positives = 34/61 (55%)
Frame = +1
Query: 97 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNTSILWGS 276
L +P NKG AFT +ER + G LP V + E QV K + ++Y++ T+IL S
Sbjct: 32 LNNPRFNKGSAFTHQERSEFALRGRLPYAVDSLEIQV---KRAYEQYKS--RETNILKNS 86
Query: 277 W 279
+
Sbjct: 87 F 87
>UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 629
Score = 50.4 bits (115), Expect = 4e-05
Identities = 30/100 (30%), Positives = 53/100 (53%), Gaps = 16/100 (16%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD- 428
++ +L L D+N+ LFYR + D + E++ ++YTP A + ++RRP G +I+ +
Sbjct: 108 KHAFLASLRDQNQVLFYRLMQDRLKELLGVLYTPGAAEAVAGYSSLFRRPVGCYISFPNQ 167
Query: 429 -------KGHVYDVLK--------NWPETDVRAIVVTDGE 503
+GH+ DV + N P+ + +VVTD E
Sbjct: 168 DGMRAQLEGHLTDVNRTADVAYDSNKPDDAIDLVVVTDAE 207
Score = 41.5 bits (93), Expect = 0.018
Identities = 21/44 (47%), Positives = 26/44 (59%)
Frame = +1
Query: 73 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQ 204
+ L G L P LNKG FT EERQ+ G+ G LP V + E+Q
Sbjct: 48 TNLRGSALLNTPRLNKGAGFTREERQIFGLEGFLPYDVHSLEKQ 91
>UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=15; Saccharomycetales|Rep: NAD-dependent
malic enzyme, mitochondrial precursor - Saccharomyces
cerevisiae (Baker's yeast)
Length = 669
Score = 50.0 bits (114), Expect = 5e-05
Identities = 25/82 (30%), Positives = 47/82 (57%), Gaps = 1/82 (1%)
Frame = +3
Query: 261 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 440
++ L +N+ L++ + ++ E++PI+YTPT G A + +R+P G+F+ I + +
Sbjct: 159 FMTSLRVQNKVLYFALIRRHIKELVPIIYTPTEGDAIAAYSHRFRKPEGVFLDITEPDSI 218
Query: 441 YDVLKNW-PETDVRAIVVTDGE 503
L + + DV IVV+D E
Sbjct: 219 ECRLATYGGDKDVDYIVVSDSE 240
Score = 45.2 bits (102), Expect = 0.001
Identities = 23/57 (40%), Positives = 30/57 (52%)
Frame = +1
Query: 79 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
L L P NKG AFT EER+ + LLPP+V T +EQ+E + + PL
Sbjct: 98 LESFQLLNSPLFNKGSAFTQEEREAFNLEALLPPQVNTLDEQLERSYKQLCYLKTPL 154
>UniRef50_Q9S4T5 Cluster: NAD-malate oxidoreductase homolog; n=15;
Legionellales|Rep: NAD-malate oxidoreductase homolog -
Legionella pneumophila
Length = 117
Score = 48.0 bits (109), Expect = 2e-04
Identities = 19/39 (48%), Positives = 30/39 (76%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLA 368
Q+IYL L D+N+ +FY+ ++ ++ EM+PI+YTP VG A
Sbjct: 79 QHIYLNNLHDKNQIVFYKLLSRHLGEMLPIIYTPIVGAA 117
Score = 47.6 bits (108), Expect = 3e-04
Identities = 27/59 (45%), Positives = 33/59 (55%)
Frame = +1
Query: 73 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
+ L G L P LNKG AFT EER+ G+ G LP RV+T +EQV+ L Y L
Sbjct: 19 TSLCGKPLLTTPQLNKGTAFTQEERKDFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRL 77
>UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n=1;
Zea mays|Rep: Putative NADP-dependent malic enzyme - Zea
mays (Maize)
Length = 309
Score = 44.8 bits (101), Expect = 0.002
Identities = 28/74 (37%), Positives = 36/74 (48%)
Frame = +3
Query: 285 NEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWP 464
+E LFY+ + DNV E++P VYT T G V DVLKNWP
Sbjct: 225 DERLFYKLLIDNVVELLPFVYTTT-------------------------GKVLDVLKNWP 259
Query: 465 ETDVRAIVVTDGER 506
+++ I VTD ER
Sbjct: 260 HRNIQVIFVTDSER 273
>UniRef50_UPI0000DB7FF6 Cluster: PREDICTED: hypothetical protein,
partial; n=1; Apis mellifera|Rep: PREDICTED:
hypothetical protein, partial - Apis mellifera
Length = 95
Score = 42.3 bits (95), Expect = 0.010
Identities = 19/37 (51%), Positives = 26/37 (70%)
Frame = +1
Query: 10 TYSEMERDRIGLWGSGDGQPTSGLSGLDHLKHPGLNK 120
T S ++RD++G G GD ++ L GLDHLK+P LNK
Sbjct: 59 TMSSVQRDQLGQRGHGDAMCSNLLRGLDHLKNPRLNK 95
>UniRef50_A5C6I9 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep:
Malic enzyme - Vitis vinifera (Grape)
Length = 498
Score = 38.7 bits (86), Expect = 0.13
Identities = 16/23 (69%), Positives = 19/23 (82%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GIP+GKL +Y A
Sbjct: 53 LGLGDLGVQGIGIPIGKLDMYVA 75
>UniRef50_UPI0000DA40E4 Cluster: PREDICTED: hypothetical protein;
n=2; Rattus norvegicus|Rep: PREDICTED: hypothetical
protein - Rattus norvegicus
Length = 193
Score = 34.3 bits (75), Expect = 2.7
Identities = 21/52 (40%), Positives = 27/52 (51%)
Frame = -1
Query: 558 LPTGMPIPQAPKSPRPKYVLRRLQQWRGRPSPASS*EHRIHVPYRGS**RGP 403
LPT P + P+ RP R +WRG P+P + + R P RGS RGP
Sbjct: 105 LPT--PCKRTPRRTRPAPCSAR--RWRGHPAPRAQWQRREGRPRRGSQARGP 152
>UniRef50_Q4SHK3 Cluster: Chromosome 5 SCAF14581, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 5 SCAF14581, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 72
Score = 33.9 bits (74), Expect = 3.6
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = -2
Query: 218 LHSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLV 69
L S SS +RG P P SS ++ R G RWSRP RP++
Sbjct: 22 LKGSAASSAAASRGQKTPASPTVPGSSQLQT----RSGQARWSRPRRPML 67
>UniRef50_A1CKF3 Cluster: Stress response protein (Ish1), putative;
n=5; Pezizomycotina|Rep: Stress response protein (Ish1),
putative - Aspergillus clavatus
Length = 516
Score = 33.9 bits (74), Expect = 3.6
Identities = 20/53 (37%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -2
Query: 410 EAPW-SAVHKPELLTGQANRRSVHNWHHFSHVIRDETVEQMLIAVQEPHKIDV 255
+A W S V KP GQA + HNWHH I D + L A + H + V
Sbjct: 63 KANWDSKVQKP---LGQAAEHTTHNWHHAKEWIFDTWSDSQLKAFLDRHGVPV 112
>UniRef50_Q4RNM6 Cluster: Chromosome 21 SCAF15012, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 21 SCAF15012, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 1187
Score = 33.5 bits (73), Expect = 4.8
Identities = 20/51 (39%), Positives = 26/51 (50%)
Frame = -2
Query: 236 YLSIESLHSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRP 84
Y S+ES + T SC L G+ +P+S RSS L RP +WS P
Sbjct: 255 YNSLESSYQRTLQSC-LKSSGSVASLPQSDRSSSSSQESLNRPLTSKWSAP 304
>UniRef50_UPI0001560FE5 Cluster: PREDICTED: similar to KIAA2007
protein; n=2; Equus caballus|Rep: PREDICTED: similar to
KIAA2007 protein - Equus caballus
Length = 745
Score = 33.1 bits (72), Expect = 6.3
Identities = 19/49 (38%), Positives = 24/49 (48%)
Frame = -2
Query: 203 CSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLVGCPS 57
C+S V + P+ RSS +PLLRP LR+S P P C S
Sbjct: 167 CASLVRASASPSRLFPRR-RSSSAVRVPLLRPLALRFSGPATPPCACES 214
>UniRef50_Q7XJP0 Cluster: SNF2/SWI2 family global transcription
factor; n=1; Arabidopsis thaliana|Rep: SNF2/SWI2 family
global transcription factor - Arabidopsis thaliana
(Mouse-ear cress)
Length = 1648
Score = 33.1 bits (72), Expect = 6.3
Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 2/87 (2%)
Frame = +1
Query: 16 SEMERDRIG--LWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVK 189
+++ D +G +W + P G+SGL HL + F + R + G GLL PRV+
Sbjct: 135 NKVSDDEVGSVIWSASFDGPGEGVSGLAHL------ASIKF-LTLRLMPGNEGLLSPRVR 187
Query: 190 TQEEQVELCKLSIDRYENPLNNTSILW 270
VE+ + + D ++ L NT +W
Sbjct: 188 -----VEMLQQAFDACDSLLENTRQIW 209
>UniRef50_Q7QQ11 Cluster: GLP_227_10956_9892; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_227_10956_9892 - Giardia lamblia
ATCC 50803
Length = 354
Score = 33.1 bits (72), Expect = 6.3
Identities = 32/119 (26%), Positives = 54/119 (45%)
Frame = -2
Query: 431 LIVDRDEEAPWSAVHKPELLTGQANRRSVHNWHHFSHVIRDETVEQMLIAVQEPHKIDVL 252
L VD + WSA+ +P + N+R H F+ ++++ + +V + L
Sbjct: 159 LFVDMFDNIVWSAI-RPCVFVLAFNKRE-HIV--FTVTLKEDQSAKDAYSVLVHDDVHKL 214
Query: 251 LSGFSYLSIESLHSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERP 75
SG S LSI ++ S+ SCV NP K + ++++ L+R SRP P
Sbjct: 215 ASGLSALSIANITSTDLDSCVCCISSVNPLESKDGLINKIQSLLLIRA-----SRPTTP 268
>UniRef50_Q1EI20 Cluster: Putative uncharacterized protein; n=2;
root|Rep: Putative uncharacterized protein - uncultured
organism
Length = 302
Score = 32.7 bits (71), Expect = 8.3
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +3
Query: 405 GLFITIHDKGHVYDVLKNWP 464
G+++TI + G + D+LKNWP
Sbjct: 210 GVYLTIREPGQITDILKNWP 229
>UniRef50_A0WC26 Cluster: Multi-sensor hybrid histidine kinase
precursor; n=2; Geobacter lovleyi SZ|Rep: Multi-sensor
hybrid histidine kinase precursor - Geobacter lovleyi SZ
Length = 1007
Score = 32.7 bits (71), Expect = 8.3
Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 360 GLACQKFGLVYRRPRGL-FITIHDKGHVYDVLKNWPETDVRAIVVTDG 500
G+ K +YR R FI HD+ H+++ +KN PE + IV DG
Sbjct: 198 GIFDSKGVFLYRTARANEFIGKHDQPHLFEQMKNGPEEGIIDIVSNDG 245
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 769,818,226
Number of Sequences: 1657284
Number of extensions: 17576327
Number of successful extensions: 55733
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 52458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 55689
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 51652897375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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