Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0324
(672 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 129 2e-30
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 126 1e-29
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 124 5e-29
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 118 3e-27
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 98 5e-21
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 93 2e-19
At2g40770.1 68415.m05030 SNF2 domain-containing protein / helica... 33 0.17
At5g59080.1 68418.m07402 expressed protein 28 4.9
At2g33490.1 68415.m04105 hydroxyproline-rich glycoprotein family... 28 6.5
At1g40115.1 68414.m04755 hypothetical protein 27 8.6
At1g09790.1 68414.m01098 phytochelatin synthetase-related contai... 27 8.6
At1g08620.1 68414.m00955 transcription factor jumonji (jmj) fami... 27 8.6
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 129 bits (311), Expect = 2e-30
Identities = 62/124 (50%), Positives = 81/124 (65%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ L L +RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP+GLFI++ DK
Sbjct: 98 KYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDK 157
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G + DVLKNWPE +++ IVVTDGER GG++PS L
Sbjct: 158 GKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSA-CL 216
Query: 612 PITI 623
P+TI
Sbjct: 217 PVTI 220
Score = 43.6 bits (98), Expect = 1e-04
Identities = 24/56 (42%), Positives = 33/56 (58%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
SG L+ P NKG+AFT +ER + GLLPP V Q+ Q + +I +Y+ PL
Sbjct: 41 SGYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPL 96
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 126 bits (304), Expect = 1e-29
Identities = 60/124 (48%), Positives = 81/124 (65%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+GL+I++ +K
Sbjct: 105 RYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEK 164
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G + +VLKNWP+ ++ IVVTDGER GGI+PS L
Sbjct: 165 GKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSA-CL 223
Query: 612 PITI 623
PITI
Sbjct: 224 PITI 227
Score = 44.0 bits (99), Expect = 9e-05
Identities = 25/60 (41%), Positives = 34/60 (56%)
Frame = +1
Query: 70 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
TS SG ++ P NKG+AFT +ER I GLLPP V +Q+ Q ++ +Y PL
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPL 103
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 124 bits (299), Expect = 5e-29
Identities = 58/124 (46%), Positives = 82/124 (66%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL+I++++K
Sbjct: 105 RYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEK 164
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G + +VLKNWP+ ++ IVVTDGER GGI+PS L
Sbjct: 165 GKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSA-CL 223
Query: 612 PITI 623
PITI
Sbjct: 224 PITI 227
Score = 42.7 bits (96), Expect = 2e-04
Identities = 23/60 (38%), Positives = 34/60 (56%)
Frame = +1
Query: 70 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
TS SG ++ P NKG+AFT +ER + GLLPP + +Q+ Q ++ +Y PL
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPL 103
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 118 bits (285), Expect = 3e-27
Identities = 53/124 (42%), Positives = 81/124 (65%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ +M L + NE LFY+ + D+V E++P++YTPTVG ACQK+G ++ RP+GLFI++ +K
Sbjct: 163 KYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEK 222
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERXXXXXXXXXXXXXSLWANSRSTPRFGGIKPSINGL 611
G +++VL+NWPE +++ IVVTDGER GG++PS L
Sbjct: 223 GKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSA-CL 281
Query: 612 PITI 623
P+TI
Sbjct: 282 PVTI 285
Score = 44.0 bits (99), Expect = 9e-05
Identities = 23/56 (41%), Positives = 34/56 (60%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
SG L+ P NKG+AF+ ER + GLLPP V +Q+ QV+ ++ +Y+ PL
Sbjct: 106 SGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPL 161
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 97.9 bits (233), Expect = 5e-21
Identities = 40/78 (51%), Positives = 54/78 (69%)
Frame = +3
Query: 273 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 452
L DRNE L+YR + DN+ + PI+YTPTVGL CQ + +YRRPRG++ + DKG + ++
Sbjct: 112 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMI 171
Query: 453 KNWPETDVRAIVVTDGER 506
NWP V IV+TDG R
Sbjct: 172 YNWPAPQVDMIVITDGSR 189
Score = 46.0 bits (104), Expect = 2e-05
Identities = 26/58 (44%), Positives = 31/58 (53%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNT 258
G D L P NK F + ER LGI GLLPPRV T +Q C I+ + + NNT
Sbjct: 39 GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMTCVQQ---CDRFIESFRSLENNT 93
Score = 38.7 bits (86), Expect = 0.003
Identities = 16/23 (69%), Positives = 19/23 (82%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GIP+GKL +Y A
Sbjct: 191 LGLGDLGVQGIGIPIGKLDMYVA 213
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 92.7 bits (220), Expect = 2e-19
Identities = 38/78 (48%), Positives = 54/78 (69%)
Frame = +3
Query: 273 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 452
L DRNE ++Y+ + +N+ E PIVYTPTVGL CQ + ++RRPRG++ + D+G + ++
Sbjct: 119 LHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMV 178
Query: 453 KNWPETDVRAIVVTDGER 506
NWP V IVVTDG R
Sbjct: 179 YNWPAEQVDMIVVTDGSR 196
Score = 48.4 bits (110), Expect = 4e-06
Identities = 24/52 (46%), Positives = 29/52 (55%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYE 240
GLD L P NKG AFT+ ER L + GLLPP V E+Q+ + R E
Sbjct: 46 GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLE 97
Score = 36.7 bits (81), Expect = 0.014
Identities = 17/23 (73%), Positives = 18/23 (78%)
Frame = +2
Query: 509 LGLGDLGACGMGIPVGKLALYTA 577
LGLGDLG G+GI VGKL LY A
Sbjct: 198 LGLGDLGVHGIGIAVGKLDLYVA 220
>At2g40770.1 68415.m05030 SNF2 domain-containing protein / helicase
domain-containing protein / zinc finger (C3HC4 type RING
finger) family protein low similarity to SP|P36607 DNA
repair protein rad8 {Schizosaccharomyces pombe};
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00628: PHD-finger, PF00097: Zinc finger, C3HC4
type (RING finger)
Length = 1648
Score = 33.1 bits (72), Expect = 0.17
Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 2/87 (2%)
Frame = +1
Query: 16 SEMERDRIG--LWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVK 189
+++ D +G +W + P G+SGL HL + F + R + G GLL PRV+
Sbjct: 135 NKVSDDEVGSVIWSASFDGPGEGVSGLAHL------ASIKF-LTLRLMPGNEGLLSPRVR 187
Query: 190 TQEEQVELCKLSIDRYENPLNNTSILW 270
VE+ + + D ++ L NT +W
Sbjct: 188 -----VEMLQQAFDACDSLLENTRQIW 209
>At5g59080.1 68418.m07402 expressed protein
Length = 135
Score = 28.3 bits (60), Expect = 4.9
Identities = 21/65 (32%), Positives = 27/65 (41%)
Frame = +1
Query: 43 LWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKL 222
L+GS D P S SG+ P +KG A G + QE++VE C L
Sbjct: 21 LFGSKDPSPPSSSSGIFSTMFPHPSKGSA-RDGSNSKHGSQAQRRESLNAQEDRVEPCHL 79
Query: 223 SIDRY 237
S Y
Sbjct: 80 SSSLY 84
>At2g33490.1 68415.m04105 hydroxyproline-rich glycoprotein family
protein Common family member:At3g26910 [Arabidopsis
thaliana]
Length = 623
Score = 27.9 bits (59), Expect = 6.5
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +1
Query: 592 SPQSMVYPSLSNVGTNNPVHALTNPV 669
SP S P NVG++NP A+T +
Sbjct: 372 SPSSTTSPGHKNVGSSNPTKAITKQI 397
>At1g40115.1 68414.m04755 hypothetical protein
Length = 150
Score = 27.5 bits (58), Expect = 8.6
Identities = 19/58 (32%), Positives = 31/58 (53%)
Frame = -2
Query: 215 HSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLVGCPSPEPHN 42
HS+T SS RG ++ P + RSS + + + RP ++RP + PSP+ H+
Sbjct: 19 HSTTRSS----RGHHHFTSPLAPRSSAI--ISITRPSLDHFTRPRGRVPSSPSPDRHS 70
>At1g09790.1 68414.m01098 phytochelatin synthetase-related contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 454
Score = 27.5 bits (58), Expect = 8.6
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +1
Query: 169 LLPPRVKTQEEQVELCKLSIDRYENPLNNTSILWG 273
+L P +K+ ++ S+ Y+N +N+T + WG
Sbjct: 328 VLHPNLKSVQQVFSFNYKSLTPYQNSINDTGMFWG 362
>At1g08620.1 68414.m00955 transcription factor jumonji (jmj) family
protein / zinc finger (C5HC2 type) family protein
contains Pfam domains, PF02375: jmjN domain, PF02373:
jmjC domain and PF02928: C5HC2 zinc finger
Length = 1183
Score = 27.5 bits (58), Expect = 8.6
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 2/70 (2%)
Frame = +1
Query: 52 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKL--S 225
+G G P S +G++ L+ G G FT+++ Q K++ + CK+ S
Sbjct: 218 NGMGDPCSASTGMNELETFGFEPGPGFTLKDFQKYADEFKAQYFKKSETSTDDKCKVDNS 277
Query: 226 IDRYENPLNN 255
ID +E L +
Sbjct: 278 IDCWEPALED 287
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,410,584
Number of Sequences: 28952
Number of extensions: 377195
Number of successful extensions: 1099
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1094
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1422784080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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