Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0311
(668 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 135 3e-32
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 135 3e-32
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 134 5e-32
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 134 5e-32
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 47 1e-05
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 39 0.003
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 39 0.003
At3g63360.1 68416.m07131 defensin-related contains weak similari... 28 4.9
At5g23575.1 68418.m02766 transmembrane protein, putative similar... 28 6.5
At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein con... 28 6.5
At3g53690.1 68416.m05929 zinc finger (C3HC4-type RING finger) fa... 28 6.5
At1g56450.1 68414.m06492 20S proteasome beta subunit G1 (PBG1) (... 28 6.5
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 135 bits (326), Expect = 3e-32
Identities = 70/136 (51%), Positives = 82/136 (60%)
Frame = +1
Query: 259 VVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLK 438
+V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR V TA TLLK
Sbjct: 64 IVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLK 123
Query: 439 RMLFRYQGHIGAALVLGGVDRTGLIFTAFILMDL*INYHMQPWDLDPLAAMAVFEAGWKR 618
+ LF YQGH+ AALVLGGVD TG LAAM+VFEA +K
Sbjct: 124 KHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKE 183
Query: 619 DMNEEEGKKACPRCYC 666
+ +EG K C
Sbjct: 184 GLTRDEGIKLVAEAIC 199
Score = 73.7 bits (173), Expect = 1e-13
Identities = 37/61 (60%), Positives = 43/61 (70%)
Frame = +2
Query: 71 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRAT 250
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVILGADTRAT
Sbjct: 1 MSQSSVDIPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRAT 60
Query: 251 E 253
E
Sbjct: 61 E 61
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 135 bits (326), Expect = 3e-32
Identities = 70/136 (51%), Positives = 82/136 (60%)
Frame = +1
Query: 259 VVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLK 438
+V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR V TA TLLK
Sbjct: 64 IVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLK 123
Query: 439 RMLFRYQGHIGAALVLGGVDRTGLIFTAFILMDL*INYHMQPWDLDPLAAMAVFEAGWKR 618
+ LF YQGH+ AALVLGGVD TG LAAM+VFEA +K
Sbjct: 124 KHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKE 183
Query: 619 DMNEEEGKKACPRCYC 666
+ +EG K C
Sbjct: 184 GLTRDEGIKLVAEAIC 199
Score = 73.7 bits (173), Expect = 1e-13
Identities = 37/61 (60%), Positives = 43/61 (70%)
Frame = +2
Query: 71 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRAT 250
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVILGADTRAT
Sbjct: 1 MSQSSVDIPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRAT 60
Query: 251 E 253
E
Sbjct: 61 E 61
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 134 bits (324), Expect = 5e-32
Identities = 70/136 (51%), Positives = 82/136 (60%)
Frame = +1
Query: 259 VVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLK 438
+V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR V TA TLLK
Sbjct: 64 IVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLK 123
Query: 439 RMLFRYQGHIGAALVLGGVDRTGLIFTAFILMDL*INYHMQPWDLDPLAAMAVFEAGWKR 618
+ LF YQGH+ AALVLGGVD TG LAAM+VFEA +K
Sbjct: 124 KHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKE 183
Query: 619 DMNEEEGKKACPRCYC 666
+ +EG K C
Sbjct: 184 GLTRDEGIKLVAESIC 199
Score = 74.5 bits (175), Expect = 6e-14
Identities = 37/61 (60%), Positives = 43/61 (70%)
Frame = +2
Query: 71 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRAT 250
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVILGADTRAT
Sbjct: 1 MSQSTVDVPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRAT 60
Query: 251 E 253
E
Sbjct: 61 E 61
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 134 bits (324), Expect = 5e-32
Identities = 70/136 (51%), Positives = 82/136 (60%)
Frame = +1
Query: 259 VVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLK 438
+V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR V TA TLLK
Sbjct: 64 IVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLK 123
Query: 439 RMLFRYQGHIGAALVLGGVDRTGLIFTAFILMDL*INYHMQPWDLDPLAAMAVFEAGWKR 618
+ LF YQGH+ AALVLGGVD TG LAAM+VFEA +K
Sbjct: 124 KHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKE 183
Query: 619 DMNEEEGKKACPRCYC 666
+ +EG K C
Sbjct: 184 GLTRDEGIKLVAESIC 199
Score = 74.5 bits (175), Expect = 6e-14
Identities = 37/61 (60%), Positives = 43/61 (70%)
Frame = +2
Query: 71 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRAT 250
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVILGADTRAT
Sbjct: 1 MSQSTVDVPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRAT 60
Query: 251 E 253
E
Sbjct: 61 E 61
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 47.2 bits (107), Expect = 1e-05
Identities = 21/80 (26%), Positives = 44/80 (55%)
Frame = +1
Query: 262 VSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKR 441
V+++ KI L N+Y C +G+AAD+++ + V L + G+ V+ +A L++
Sbjct: 38 VANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRM 97
Query: 442 MLFRYQGHIGAALVLGGVDR 501
+ + + + L++GG D+
Sbjct: 98 LAYNNKNMLQTGLIVGGWDK 117
Score = 34.3 bits (75), Expect = 0.074
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +2
Query: 185 GTTIVGIIYADGVILGADTRAT 250
GTTI+G+ Y GV+LGAD+R +
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTS 33
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 38.7 bits (86), Expect = 0.003
Identities = 24/83 (28%), Positives = 41/83 (49%), Gaps = 1/83 (1%)
Frame = +1
Query: 262 VSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKR 441
+S ++ +KI + M AG AAD + +++ + L L R + V A+ LL
Sbjct: 83 ISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLAN 142
Query: 442 MLFRYQG-HIGAALVLGGVDRTG 507
ML+ Y+G + ++ G D TG
Sbjct: 143 MLYSYRGMGLSVGTMIAGWDETG 165
Score = 29.5 bits (63), Expect = 2.1
Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 3/67 (4%)
Frame = +2
Query: 59 SNHEMASAL--VPEIPVPGFS-FENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVIL 229
SN EM P +P + F+ FQ+ A K PA GTT + I+ +GV++
Sbjct: 19 SNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVK--PAK-----GTTTLAFIFKEGVMV 71
Query: 230 GADTRAT 250
AD+RA+
Sbjct: 72 AADSRAS 78
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 38.7 bits (86), Expect = 0.003
Identities = 24/83 (28%), Positives = 41/83 (49%), Gaps = 1/83 (1%)
Frame = +1
Query: 262 VSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKR 441
+S ++ +KI + M AG AAD + +++ + L L R + V A+ LL
Sbjct: 83 ISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLAN 142
Query: 442 MLFRYQG-HIGAALVLGGVDRTG 507
ML+ Y+G + ++ G D TG
Sbjct: 143 MLYSYRGMGLSVGTMIAGWDETG 165
>At3g63360.1 68416.m07131 defensin-related contains weak similarity
to defensin [Capsicum annuum] gi|1200228|emb|CAA65045
Length = 85
Score = 28.3 bits (60), Expect = 4.9
Identities = 19/68 (27%), Positives = 31/68 (45%), Gaps = 3/68 (4%)
Frame = -2
Query: 382 ATPIVKQQIVLSFQCQL-LFLLHNNTCWMLSSG--FFGSFCPRQLYFSGSCVSS*NNAVC 212
+T ++KQ + +C+ LL N CW+ +G + C + SG CVS + +C
Sbjct: 18 STGLMKQGDAQAQKCEWECKLLPNFPCWLKGAGEGLCDNLCKYEGAISGVCVSDPHRCLC 77
Query: 211 VYYTNNCS 188
CS
Sbjct: 78 RNRKPGCS 85
>At5g23575.1 68418.m02766 transmembrane protein, putative similar to
cleft lip and palate transmembrane protein 1 [Homo
sapiens] GI:4039014; contains Pfam profile PF05602:
Cleft lip and palate transmembrane protein 1 (CLPTM1)
Length = 593
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 5/31 (16%)
Frame = -2
Query: 364 QQIVLSFQCQL---LFLLHNNTCWML--SSG 287
+ +VL+F CQ L+LL N+T WM+ SSG
Sbjct: 368 KSVVLNFICQFVIFLYLLDNDTSWMILASSG 398
>At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein
contains Pfam profile PF05602: Cleft lip and palate
transmembrane protein 1 (CLPTM1)
Length = 590
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 5/31 (16%)
Frame = -2
Query: 364 QQIVLSFQCQL---LFLLHNNTCWML--SSG 287
+ +VL+F CQ L+LL N+T WM+ SSG
Sbjct: 366 KSVVLNFICQFIIFLYLLDNDTSWMILASSG 396
>At3g53690.1 68416.m05929 zinc finger (C3HC4-type RING finger)
family protein contains a Zinc finger, C3HC4 type (RING
finger) signature, PROSITE:PS00518 and a IBR domain,
Pfam:PF01485
Length = 320
Score = 27.9 bits (59), Expect = 6.5
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = +3
Query: 366 FTIGVAALTYWTYCTSRNCCHTAETNAISLSRPYRGCSG 482
F IG + Y C S+ + N +S+ P GCSG
Sbjct: 128 FRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSG 166
>At1g56450.1 68414.m06492 20S proteasome beta subunit G1 (PBG1)
(PRCH) identical to 20S proteasome beta subunit (PBG1)
GI:3421123 [Arabidopsis thaliana]; identical to cDNA
proteasome subunit prch GI:2511597
Length = 246
Score = 27.9 bits (59), Expect = 6.5
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 182 TGTTIVGIIYADGVILGAD 238
TGT+IV I Y DGV++ +D
Sbjct: 30 TGTSIVAIKYKDGVLMASD 48
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,546,425
Number of Sequences: 28952
Number of extensions: 308646
Number of successful extensions: 813
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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