BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0309 (603 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 216 2e-57 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 128 5e-31 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 120 2e-28 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 112 4e-26 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 71 1e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 68 9e-13 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 57 2e-09 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 48 1e-06 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.074 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.30 SPCC594.04c |||steroid oxidoreductase superfamily protein|Schizo... 28 1.2 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.8 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 27 2.8 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 3.7 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.7 SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 26 4.9 SPAC630.09c |mug58||glycerate kinase |Schizosaccharomyces pombe|... 25 6.4 SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 25 8.5 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 216 bits (528), Expect = 2e-57 Identities = 99/116 (85%), Positives = 104/116 (89%) Frame = +1 Query: 250 QKGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 429 ++GILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT Sbjct: 61 KRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMT 120 Query: 430 QIMFETFNTPAMYVAIQAVLSLYAFGLPPVFVLDSRDGVFHTVPIYEGYALPHAIL 597 QI+FETFN PA YVAIQAVLSLYA G VLDS DGV HTVPIYEGYALPHAI+ Sbjct: 121 QIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIM 176 Score = 121 bits (291), Expect = 9e-29 Identities = 53/58 (91%), Positives = 57/58 (98%) Frame = +2 Query: 74 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 247 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQ Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQ 59 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 128 bits (310), Expect = 5e-31 Identities = 56/114 (49%), Positives = 81/114 (71%) Frame = +1 Query: 253 KGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 432 + +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 433 IMFETFNTPAMYVAIQAVLSLYAFGLPPVFVLDSRDGVFHTVPIYEGYALPHAI 594 MFE + +YVAIQAVLSLYA GL V+DS DGV H VP+YE L H + Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLV 175 Score = 54.0 bits (124), Expect = 2e-08 Identities = 29/58 (50%), Positives = 37/58 (63%), Gaps = 2/58 (3%) Frame = +2 Query: 80 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQ 247 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA+ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAE 59 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 120 bits (288), Expect = 2e-28 Identities = 53/115 (46%), Positives = 79/115 (68%), Gaps = 1/115 (0%) Frame = +1 Query: 253 KGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMT 429 +G+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIA 125 Query: 430 QIMFETFNTPAMYVAIQAVLSLYAFGLPPVFVLDSRDGVFHTVPIYEGYALPHAI 594 + +ET N PA+ ++Q VL+LYA VL+ DG+ H+VPIY+G+++P AI Sbjct: 126 EYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAI 180 Score = 69.3 bits (162), Expect = 4e-13 Identities = 30/52 (57%), Positives = 38/52 (73%) Frame = +2 Query: 92 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 247 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQ 63 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 112 bits (269), Expect = 4e-26 Identities = 54/118 (45%), Positives = 71/118 (60%), Gaps = 8/118 (6%) Frame = +1 Query: 265 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 444 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 445 TFNTPAMYVAIQAVLSLYAFGLPPVF--------VLDSRDGVFHTVPIYEGYALPHAI 594 +FN +Y+A+QAVL+L A V+DS DGV H +P+ EGY + +I Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSI 206 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +2 Query: 92 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 241 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 70.9 bits (166), Expect = 1e-13 Identities = 36/107 (33%), Positives = 54/107 (50%) Frame = +1 Query: 262 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 441 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 442 ETFNTPAMYVAIQAVLSLYAFGLPPVFVLDSRDGVFHTVPIYEGYAL 582 E+ PA Y+A Q + +A G ++D IY+G+ L Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 77 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 178 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 68.1 bits (159), Expect = 9e-13 Identities = 33/101 (32%), Positives = 54/101 (53%), Gaps = 2/101 (1%) Frame = +1 Query: 277 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 456 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 457 PAMYVAIQAVLSLYAFGLPPV--FVLDSRDGVFHTVPIYEG 573 P++ I + S Y P VL+ + H +P+ G Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 92 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 187 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 56.8 bits (131), Expect = 2e-09 Identities = 26/103 (25%), Positives = 50/103 (48%) Frame = +1 Query: 286 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 465 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 466 YVAIQAVLSLYAFGLPPVFVLDSRDGVFHTVPIYEGYALPHAI 594 Y+ QAV +A ++D P+ +G + I Sbjct: 134 YLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGI 176 Score = 38.7 bits (86), Expect = 6e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +2 Query: 77 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 247 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 47.6 bits (108), Expect = 1e-06 Identities = 30/103 (29%), Positives = 46/103 (44%), Gaps = 4/103 (3%) Frame = +1 Query: 277 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 444 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 445 TFNTPAMYVAIQAVLSLYAFGLPPVFVLDSRDGVFHTVPIYEG 573 P +A + ++ LYA G+ V+D PI +G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.074 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 337 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 480 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.30 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -1 Query: 234 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 73 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPCC594.04c |||steroid oxidoreductase superfamily protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 338 Score = 27.9 bits (59), Expect = 1.2 Identities = 11/35 (31%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Frame = -2 Query: 506 PNAYSESTAWMATYMAGVLNVSNMI-WVIFSLLAL 405 PN +E W++ Y+ G + +++ W IF+ L L Sbjct: 251 PNFLAEQLIWLSFYLFGAIASESLLNWTIFAWLGL 285 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 2.8 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -1 Query: 237 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 121 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 26.6 bits (56), Expect = 2.8 Identities = 12/29 (41%), Positives = 15/29 (51%) Frame = -2 Query: 245 VPHLLHKSPSVPYRPSRPDGGAFPRSRET 159 + L+ K+PS PY SRP A S T Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPT 334 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 26.2 bits (55), Expect = 3.7 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +2 Query: 137 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 241 G+ PRA F ++ P H G+++ M KD G+E Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.7 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 379 LLTEAPLNPKANREKMTQIMFETFN 453 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 243 Score = 25.8 bits (54), Expect = 4.9 Identities = 16/46 (34%), Positives = 20/46 (43%) Frame = -2 Query: 263 RIPFCSVPHLLHKSPSVPYRPSRPDGGAFPRSRETLREEHHLLRSR 126 R PF S+ H HK YRPSR + + R + RSR Sbjct: 176 RFPFESL-HKNHKHYENSYRPSRSQNNSHYNDKSFHRSRYSRARSR 220 >SPAC630.09c |mug58||glycerate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 25.4 bits (53), Expect = 6.4 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -1 Query: 552 VEDTVAGVQHEYRW*TERVQREHGLDGDVHGG 457 VE + + YRW R+Q+EH L +H G Sbjct: 200 VELEAQEINYVYRW---RLQQEHALKARIHKG 228 >SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 25.0 bits (52), Expect = 8.5 Identities = 11/30 (36%), Positives = 14/30 (46%) Frame = -2 Query: 290 PCSMGYLRVRIPFCSVPHLLHKSPSVPYRP 201 P + Y P +VP +L PSV Y P Sbjct: 349 PLQLSYAHYATPLPAVPTVLSGLPSVAYTP 378 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,689,273 Number of Sequences: 5004 Number of extensions: 59393 Number of successful extensions: 187 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 182 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 264253462 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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