SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0292
         (480 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   108   5e-25
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   103   1e-23
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    67   1e-12
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    50   2e-07
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr...    28   0.84 
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po...    27   1.5  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   3.4  
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos...    25   4.5  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   4.5  
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce...    25   4.5  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    25   5.9  
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos...    25   5.9  
SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac...    25   7.8  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  108 bits (259), Expect = 5e-25
 Identities = 55/133 (41%), Positives = 69/133 (51%)
 Frame = +1

Query: 82  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 261
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG   
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 262 TYPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLLLVRKXXXXXXXXXXXXLERKSVDLVLD 441
             P                       F    L+  ++            + ++ VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 442 RIRKLADQCTGLQ 480
           +IR++AD C+GLQ
Sbjct: 121 KIRRIADNCSGLQ 133



 Score =  107 bits (258), Expect = 6e-25
 Identities = 45/66 (68%), Positives = 54/66 (81%)
 Frame = +3

Query: 225 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAPGHY 404
           F  F      GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYA GHY
Sbjct: 49  FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108

Query: 405 TIGKEI 422
           T+GKE+
Sbjct: 109 TVGKEL 114


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  103 bits (247), Expect = 1e-23
 Identities = 58/138 (42%), Positives = 72/138 (52%), Gaps = 5/138 (3%)
 Frame = +1

Query: 82  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 246
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 247 TGAASTYPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLLLVRKXXXXXXXXXXXXLERKSV 426
           TG     P                       F    ++  ++            + ++ +
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 427 DLVLDRIRKLADQCTGLQ 480
           D VL+RIR++AD C+GLQ
Sbjct: 120 DSVLERIRRMADNCSGLQ 137



 Score =  103 bits (246), Expect = 2e-23
 Identities = 42/66 (63%), Positives = 53/66 (80%)
 Frame = +3

Query: 225 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAPGHY 404
           F  F      GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYA GHY
Sbjct: 53  FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112

Query: 405 TIGKEI 422
           T+GKE+
Sbjct: 113 TVGKEM 118


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 67.3 bits (157), Expect = 1e-12
 Identities = 29/57 (50%), Positives = 40/57 (70%)
 Frame = +3

Query: 255 GKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAPGHYTIGKEIS 425
           GK+VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A GHYT G E++
Sbjct: 57  GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELA 113



 Score = 41.5 bits (93), Expect = 6e-05
 Identities = 30/133 (22%), Positives = 46/133 (34%)
 Frame = +1

Query: 82  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 261
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E     
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 262 TYPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLLLVRKXXXXXXXXXXXXLERKSVDLVLD 441
             P                     + F  +N++  +                +  D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 442 RIRKLADQCTGLQ 480
            +R+ A+ C  LQ
Sbjct: 119 VVRREAEACDALQ 131


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 49.6 bits (113), Expect = 2e-07
 Identities = 23/58 (39%), Positives = 38/58 (65%), Gaps = 2/58 (3%)
 Frame = +3

Query: 234 FLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYAPGH 401
           F Q D + +++PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G+
Sbjct: 52  FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGY 108



 Score = 48.8 bits (111), Expect = 4e-07
 Identities = 24/55 (43%), Positives = 33/55 (60%)
 Frame = +1

Query: 85  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 249
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55


>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1024

 Score = 27.9 bits (59), Expect = 0.84
 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
 Frame = -2

Query: 317 ADLINNSRFKIDEDS--TGYVLAAPVSLKKVLKESSP 213
           AD+IN SR+ ID  S  T +    P+SL    KE  P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491


>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 399

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
 Frame = -2

Query: 350 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIW 183
           F    + +  S  +++N+ +  KID    GY      S K  + E +  P  +  G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 3.4
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +3

Query: 180 WPDAHRQDHRGWRRFFQHFLQR 245
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPBC119.07 |ppk19||serine/threonine protein kinase
           Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1706

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 13/40 (32%), Positives = 19/40 (47%)
 Frame = +3

Query: 183 PDAHRQDHRGWRRFFQHFLQRDRSGKHVPRAVFVDLEPTV 302
           P+ H +   G   F + F  +DR G  V   VFV+  P +
Sbjct: 22  PEYHNERSLGDSHFLRTFRMQDRKGYDVLIKVFVNKLPEI 61


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +2

Query: 71  LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 193
           L+S        +L+ P SR++ P  S +  STASSL    P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1067

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = -2

Query: 284 DEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIWPSG 174
           ++D T Y L  P+   + L +++PP +V+ +G  P G
Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 14/30 (46%), Positives = 17/30 (56%)
 Frame = +2

Query: 161 STASSLMARCPQTRPSGVETILSTLSSARP 250
           ST SSL +    ++PS   T  ST SSA P
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAP 202


>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 400

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = -1

Query: 135 ITDLDSGLANVYRDALTHFDLSF 67
           +T +D GLA  YRD  TH  + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168


>SPBC3H7.15 |hhp1||serine/threonine protein kinase
           Hhp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 365

 Score = 24.6 bits (51), Expect = 7.8
 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
 Frame = -1

Query: 189 HLAIRLDAVLQAV-KLPAGITDLDSGLANVYRDALTHFDLSF 67
           H  I+ D  L  + K    +  +D GLA  YRD  TH  + +
Sbjct: 128 HRDIKPDNFLMGIGKRGNQVNIIDFGLAKKYRDHKTHLHIPY 169


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,008,604
Number of Sequences: 5004
Number of extensions: 40151
Number of successful extensions: 136
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -