BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0289 (539 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 28 0.77 SPAC1A6.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 1.3 SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 26 3.1 SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 25 5.4 SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 25 5.4 SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|... 25 7.2 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 25 9.5 SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 25 9.5 SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces p... 25 9.5 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 28.3 bits (60), Expect = 0.77 Identities = 15/37 (40%), Positives = 21/37 (56%) Frame = -1 Query: 374 RSHAVTAASLHCLGSFGTPRRSCSFSQEAKVAFSASA 264 R+ + T AS+ LGS P RS S++ KVA S+ Sbjct: 356 RAQSTTVASVKHLGSAWLPPRSLSYNPAEKVALLTSS 392 >SPAC1A6.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 636 Score = 27.5 bits (58), Expect = 1.3 Identities = 9/20 (45%), Positives = 16/20 (80%) Frame = -3 Query: 162 ARGSSASWGSHFMPSALPPP 103 +RGS A++ + + P+A+PPP Sbjct: 199 SRGSPANYNTPYYPTAIPPP 218 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 26.2 bits (55), Expect = 3.1 Identities = 10/23 (43%), Positives = 11/23 (47%) Frame = -1 Query: 296 QEAKVAFSASAIFLWCPGSVAAP 228 Q+AK F FLWCP P Sbjct: 896 QKAKKDFVQDGFFLWCPSKDVVP 918 >SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1079 Score = 25.4 bits (53), Expect = 5.4 Identities = 9/24 (37%), Positives = 17/24 (70%) Frame = +2 Query: 134 EPQEAEEPRAMQKVPSLSDLSDPE 205 EPQE E P+ +++ ++++L D E Sbjct: 121 EPQEIESPQGPKELTAVTELDDDE 144 >SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 756 Score = 25.4 bits (53), Expect = 5.4 Identities = 21/60 (35%), Positives = 30/60 (50%) Frame = +3 Query: 279 GHLRLLGEGAAAPRRAKRSKTVERSSSDGVAALGGSRVLIRGCRPLTIRASSGQGHLCDG 458 G L+ +G G PR A R+ +SS+ + A GG + +RG R SSG H+ G Sbjct: 481 GMLKNVGNG---PRNAPRN-----NSSNNLHAQGGMPMNMRGPRGAPRNNSSGDLHIQSG 532 >SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|Schizosaccharomyces pombe|chr 2|||Manual Length = 624 Score = 25.0 bits (52), Expect = 7.2 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -1 Query: 74 TRHASLEEAPRRSEPRKRL 18 T H L+E+P SEP K L Sbjct: 241 TYHLYLDESPEESEPEKEL 259 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 24.6 bits (51), Expect = 9.5 Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 3/22 (13%) Frame = -3 Query: 531 FVRSLDVP---KYVSHKCGRSG 475 FV LD P K SH+CGR+G Sbjct: 330 FVLQLDPPLDPKSFSHRCGRAG 351 >SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 24.6 bits (51), Expect = 9.5 Identities = 10/34 (29%), Positives = 16/34 (47%) Frame = +2 Query: 425 RELRARTSVRWAARSSWPERPHLWETYFGTSRDL 526 + + RT A WP+ + +FGT +DL Sbjct: 89 KNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQDL 122 >SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 464 Score = 24.6 bits (51), Expect = 9.5 Identities = 13/39 (33%), Positives = 19/39 (48%) Frame = +1 Query: 250 HHKKMAEALKATFASWEKEQLRLGVPKDPRQWSEAAVTA 366 HHK ALK + E+E+ RL P + +A + A Sbjct: 426 HHKDYKHALKKRITALEEEKNRLIKEIGPEKVKKAGILA 464 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,190,509 Number of Sequences: 5004 Number of extensions: 41601 Number of successful extensions: 131 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 221892220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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