BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0275 (722 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 155 6e-39 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 6e-38 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 98 1e-21 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 66 4e-12 SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 29 0.67 SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 27 2.7 SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 26 4.7 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.3 SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 25 8.3 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 25 8.3 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.3 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 155 bits (376), Expect = 6e-39 Identities = 65/92 (70%), Positives = 79/92 (85%) Frame = +2 Query: 233 FQHFLQRAGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 412 F F G GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108 Query: 413 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHS 508 T+GKE+VD V D+IR++AD C+GLQGFL+FHS Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHS 140 Score = 88.6 bits (210), Expect = 8e-19 Identities = 38/55 (69%), Positives = 42/55 (76%) Frame = +3 Query: 90 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSE 254 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSE Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSE 55 Score = 87.0 bits (206), Expect = 2e-18 Identities = 38/58 (65%), Positives = 47/58 (81%) Frame = +1 Query: 547 MERLFVDYGKKSKLEFGIYPAPQVSTGVVEPYNFILTTHTTLEHSDWAFMGDNEALYD 720 +ERL ++Y KKSKL+F +YPAPQVST VVEPYN +LTTH TL+ +D FM DNE+ YD Sbjct: 154 LERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYD 211 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 152 bits (368), Expect = 6e-38 Identities = 62/92 (67%), Positives = 79/92 (85%) Frame = +2 Query: 233 FQHFLQRAGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 412 F F G GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112 Query: 413 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHS 508 T+GKE++D VL+RIR++AD C+GLQGFL+FHS Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHS 144 Score = 91.9 bits (218), Expect = 8e-20 Identities = 40/58 (68%), Positives = 48/58 (82%) Frame = +1 Query: 547 MERLFVDYGKKSKLEFGIYPAPQVSTGVVEPYNFILTTHTTLEHSDWAFMGDNEALYD 720 +ERL ++YGKKS L+F +YPAPQVST VVEPYN +LTTH TL++SD FM DNEA YD Sbjct: 158 LERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYD 215 Score = 82.6 bits (195), Expect = 5e-17 Identities = 42/60 (70%), Positives = 44/60 (73%), Gaps = 5/60 (8%) Frame = +3 Query: 90 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 254 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 97.9 bits (233), Expect = 1e-21 Identities = 43/89 (48%), Positives = 59/89 (66%) Frame = +2 Query: 242 FLQRAGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIG 421 + A GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G Sbjct: 50 YFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEG 109 Query: 422 KEIVDLVLDRIRKLADQCTGLQGFLIFHS 508 E+ D VLD +R+ A+ C LQGF + HS Sbjct: 110 AELADAVLDVVRREAEACDALQGFQLTHS 138 Score = 48.4 bits (110), Expect = 1e-06 Identities = 23/56 (41%), Positives = 33/56 (58%) Frame = +1 Query: 547 MERLFVDYGKKSKLEFGIYPAPQVSTGVVEPYNFILTTHTTLEHSDWAFMGDNEAL 714 + ++ +Y + F + PAP+ S VVEPYN L+ H +E+SD F DNEAL Sbjct: 152 LSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207 Score = 34.7 bits (76), Expect = 0.014 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = +3 Query: 90 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDG 191 MRE + + GQ G Q+G A W EHG+ G Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 66.5 bits (155), Expect = 4e-12 Identities = 32/91 (35%), Positives = 55/91 (60%), Gaps = 2/91 (2%) Frame = +2 Query: 242 FLQRAGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 415 F ++ +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ Sbjct: 51 FFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109 Query: 416 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHS 508 + I + ++D I + AD L+GF + HS Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHS 140 Score = 48.8 bits (111), Expect = 8e-07 Identities = 24/56 (42%), Positives = 33/56 (58%) Frame = +3 Query: 93 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSEPE 260 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF + + Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSD 56 >SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 29.1 bits (62), Expect = 0.67 Identities = 14/58 (24%), Positives = 26/58 (44%), Gaps = 2/58 (3%) Frame = -2 Query: 358 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLASSGSLKKVLKESSPPPMVLSVGIW 191 F + + S +++N+ + KID GY + S K + E + P + G+W Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262 >SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1060 Score = 27.1 bits (57), Expect = 2.7 Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -1 Query: 275 VRACQLRLAEESVERIVSTPDG-LVCGHLAIRLDAVL 168 VR C RLA S+ +I+S G CG L + DA+L Sbjct: 957 VRVCTHRLAYNSLLKIISISQGDTACGDLVV--DAIL 991 >SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 992 Score = 26.2 bits (55), Expect = 4.7 Identities = 17/48 (35%), Positives = 23/48 (47%) Frame = -3 Query: 567 VNEETLHQ*GSEPRAGTSTEEWKIRNPCRPVHWSASLRILSKTKSTIS 424 VNE++ +Q E GTS P+H + SL LSK +IS Sbjct: 528 VNEDSANQ-KEESALGTSDNSDMYEKENTPIHHNESLNQLSKDLGSIS 574 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +2 Query: 188 WPDAHRQDHRGWRRFFQHFLQR 253 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 830 Score = 25.4 bits (53), Expect = 8.3 Identities = 14/46 (30%), Positives = 24/46 (52%) Frame = -2 Query: 577 SSRSQRRDAPSIRK*TQSRYLHRGVEDQESLQTGTLVSELADSVQN 440 SS+ SIR ++SR G + Q S+Q+GT ++D ++ Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/38 (31%), Positives = 18/38 (47%) Frame = -2 Query: 325 ADLINNSRFKIDEDSTGYVLASSGSLKKVLKESSPPPM 212 AD+IN SR+ ID S +++ L P P+ Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 8.3 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +1 Query: 79 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 201 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,005,056 Number of Sequences: 5004 Number of extensions: 61392 Number of successful extensions: 190 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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