BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0255 (712 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 159 3e-40 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 75 1e-14 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 73 3e-14 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 71 1e-13 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 59 7e-10 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 47 2e-06 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 45 9e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 45 9e-06 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.002 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.5 SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein |Schizosacch... 26 4.6 SPBC1685.13 |||non classical export pathway protein |Schizosacch... 26 6.1 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 6.1 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 6.1 SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosacchar... 25 8.1 SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p... 25 8.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 159 bits (387), Expect = 3e-40 Identities = 83/131 (63%), Positives = 95/131 (72%), Gaps = 4/131 (3%) Frame = -1 Query: 652 TMENERFPLPRGFLPTLVLGYESLR-HPRDHI*LHH---EVRRGHP*GLVRQTVLSGGTT 485 T+ NERF P LG E+ H + + ++R+ L V+SGGTT Sbjct: 249 TIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKD----LYGNVVMSGGTT 304 Query: 484 MYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 305 MYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDE Sbjct: 305 MYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 364 Query: 304 SGPSIVHRKCF 272 SGP IV+RKCF Sbjct: 365 SGPGIVYRKCF 375 Score = 28.7 bits (61), Expect = 0.87 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -3 Query: 692 LKKSYELPAGQVIHYGKRKIPVAQRL 615 L+KSYELP GQVI G + + L Sbjct: 236 LEKSYELPDGQVITIGNERFRAPEAL 261 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 74.9 bits (176), Expect = 1e-14 Identities = 36/88 (40%), Positives = 59/88 (67%), Gaps = 6/88 (6%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASL 350 L+ ++ GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL Sbjct: 346 LLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSL 404 Query: 349 STFQQMWISKQEYDESGP---SIVHRKC 275 TF Q+WIS+QEY+E G +++ ++C Sbjct: 405 GTFHQLWISRQEYEEHGSDRLALIEKRC 432 Score = 26.2 bits (55), Expect = 4.6 Identities = 13/46 (28%), Positives = 23/46 (50%) Frame = -2 Query: 654 PLWKTKDSRCPEAFFQPSFLGMKACGIHETTYNSIMKCDVDIRKDL 517 P+ +T+++ + + + GI + N I +CDVDIR L Sbjct: 301 PIEQTENNEVSQQDSSVTNTSSRILGIPQLFQNCISECDVDIRASL 346 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 73.3 bits (172), Expect = 3e-14 Identities = 34/83 (40%), Positives = 56/83 (67%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTF 341 L VLSGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF Sbjct: 298 LYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTF 357 Query: 340 QQMWISKQEYDESGPSIVHRKCF 272 +++ I+ +EY ++ +++ R+ F Sbjct: 358 RRLLITSEEY-KNDQNVIFRRRF 379 Score = 40.3 bits (90), Expect = 3e-04 Identities = 15/38 (39%), Positives = 23/38 (60%) Frame = -2 Query: 624 PEAFFQPSFLGMKACGIHETTYNSIMKCDVDIRKDLYA 511 PE F P +G +A G+H + SI+ D+D+R LY+ Sbjct: 263 PEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYS 300 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 71.3 bits (167), Expect = 1e-13 Identities = 34/76 (44%), Positives = 49/76 (64%), Gaps = 2/76 (2%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSILASLS 347 L+ V++GGT++ PG+++R+Q E+ LA S + + +VW GGSILASL Sbjct: 343 LLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLD 402 Query: 346 TFQQMWISKQEYDESG 299 FQ +W+SKQEYDE G Sbjct: 403 NFQHLWVSKQEYDEVG 418 Score = 29.9 bits (64), Expect = 0.38 Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 9/47 (19%) Frame = -2 Query: 630 RCPEAFFQPSFLGMKAC---------GIHETTYNSIMKCDVDIRKDL 517 R E F PSF ++ G+HE Y SI+ CD ++R L Sbjct: 297 RISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPL 343 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 58.8 bits (136), Expect = 7e-10 Identities = 34/86 (39%), Positives = 53/86 (61%), Gaps = 12/86 (13%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWI 374 L R VLSGG++MY G+ R++KEI L P+ + K+KI P R+++V+I Sbjct: 293 LYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFI 352 Query: 373 GGSILAS-LSTFQQMWISKQEYDESG 299 GG++LA ++ MW+SK E++E G Sbjct: 353 GGAVLADIMAQNDHMWVSKAEWEEYG 378 Score = 42.7 bits (96), Expect = 5e-05 Identities = 18/38 (47%), Positives = 24/38 (63%) Frame = -2 Query: 627 CPEAFFQPSFLGMKACGIHETTYNSIMKCDVDIRKDLY 514 CPE FQP +G + G+ E +++I DVDIRK LY Sbjct: 257 CPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLY 294 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 47.2 bits (107), Expect = 2e-06 Identities = 26/95 (27%), Positives = 47/95 (49%), Gaps = 14/95 (14%) Frame = -1 Query: 523 GLVRQTVLSGGTTMYPGIADRMQKEITALAPSTMK--------------IKIIAPPERKY 386 GL + VLSGG+T++ +R+Q+++ + + + +I+ ++ Sbjct: 325 GLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRN 384 Query: 385 SVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 281 +VW GGS+LA F +K +Y+E G SI R Sbjct: 385 AVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 45.2 bits (102), Expect = 9e-06 Identities = 24/81 (29%), Positives = 40/81 (49%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTF 341 L+ V GG +PG R+ E+ +LAP+ ++K+ P + W S + L + Sbjct: 319 LLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHW 377 Query: 340 QQMWISKQEYDESGPSIVHRK 278 I++ EY E G +I+ RK Sbjct: 378 NANKITRSEYSEHGANIMTRK 398 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 45.2 bits (102), Expect = 9e-06 Identities = 20/78 (25%), Positives = 38/78 (48%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTF 341 LV +++GG PG+ R+++E+T++ P I + W G S + F Sbjct: 634 LVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKF 693 Query: 340 QQMWISKQEYDESGPSIV 287 + ++++EY E GP + Sbjct: 694 KAAKVTREEYLEKGPEYI 711 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 37.5 bits (83), Expect = 0.002 Identities = 18/84 (21%), Positives = 37/84 (44%), Gaps = 3/84 (3%) Frame = -1 Query: 520 LVRQTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASL 350 L ++ GG +PG A +++ I + + I +I PP + ++ W G I + Sbjct: 529 LYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRI 588 Query: 349 STFQQMWISKQEYDESGPSIVHRK 278 ++WI ++ G ++ K Sbjct: 589 RIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = -1 Query: 379 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 278 ++GGSI+A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1294 Score = 26.2 bits (55), Expect = 4.6 Identities = 8/21 (38%), Positives = 15/21 (71%) Frame = -2 Query: 552 IMKCDVDIRKDLYAKPYCPVV 490 + C V++R ++YA+ CP+V Sbjct: 478 LFACPVELRPEVYARMLCPLV 498 >SPBC1685.13 |||non classical export pathway protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 183 Score = 25.8 bits (54), Expect = 6.1 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +2 Query: 545 FMMELYVVSWMPQAFIPKNE 604 FM LYV S++ Q FI KN+ Sbjct: 136 FMFGLYVASFIVQIFIAKND 155 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.8 bits (54), Expect = 6.1 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -2 Query: 387 TPYGSVDRSSPPSLPSNRCGSRNRSTTSLAPP 292 TP + PPS+PS+R R S ++ APP Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 25.8 bits (54), Expect = 6.1 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -1 Query: 454 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 344 KEI++ A S + + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.4 bits (53), Expect = 8.1 Identities = 17/62 (27%), Positives = 30/62 (48%) Frame = -3 Query: 497 RWYHHVPWNRRPYAKGNHSSRPIDNED*DHCSSREEVLRMDRWIDPRLPLYLPTDVDLET 318 RWY PW + KG+ + +E + SSR + + W+D YL +++D+ + Sbjct: 483 RWYE--PW----FVKGDLN---YTSEVKERTSSRLSAVGSENWVDDDFYEYLLSEIDMVS 533 Query: 317 GV 312 V Sbjct: 534 AV 535 >SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 25.4 bits (53), Expect = 8.1 Identities = 17/47 (36%), Positives = 21/47 (44%), Gaps = 4/47 (8%) Frame = -3 Query: 374 RWIDPRLPLYLPTDVDLETGVRRVWPLHCTQEVL----LNAPRVLPP 246 +W+D YLP V L TG W + C L L R+LPP Sbjct: 629 KWMD-----YLPRPVILVTGSIHFWSIACDDGSLHLYSLTGSRLLPP 670 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,042,393 Number of Sequences: 5004 Number of extensions: 64755 Number of successful extensions: 198 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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