BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0251 (639 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 203 1e-53 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 91 2e-19 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 87 2e-18 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 87 3e-18 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 73 3e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 52 7e-08 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 49 7e-07 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 48 9e-07 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 42 6e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.3 SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 27 2.3 SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces ... 27 3.0 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 27 3.0 SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosacchar... 26 5.3 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 5.3 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 5.3 SPBC23G7.10c |||NADH-dependent flavin oxidoreductase |Schizosacc... 25 7.0 SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|ch... 25 9.2 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 203 bits (496), Expect = 1e-53 Identities = 93/123 (75%), Positives = 101/123 (82%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G E GIHE TYNSIMKCDV+IRKDLY GGTTMYPGIADR Sbjct: 253 ERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADR 312 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+R Sbjct: 313 MQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYR 372 Query: 275 KCF 267 KCF Sbjct: 373 KCF 375 Score = 26.2 bits (55), Expect = 4.0 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMKLA 575 N+RF P+A FQP LG++ A Sbjct: 252 NERFRAPEALFQPSALGLENA 272 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 90.6 bits (215), Expect = 2e-19 Identities = 41/110 (37%), Positives = 71/110 (64%) Frame = -2 Query: 596 VFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAPSTM 417 + G EA G+H + SI+ D+++R LY+ GG+T+ G +R E+ A++ Sbjct: 271 MMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKN 330 Query: 416 KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 267 ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F Sbjct: 331 QVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 87.4 bits (207), Expect = 2e-18 Identities = 42/96 (43%), Positives = 57/96 (59%), Gaps = 2/96 (2%) Frame = -2 Query: 575 GIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAP-STMKIKIIA 399 G+HE Y SI+ CD +R L GGT++ PG+++R+Q E+ LA S + + Sbjct: 323 GLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAE 382 Query: 398 PPER-KYSVWIGGSILASLSTFQQMWISKQEYDESG 294 +VW GGSILASL FQ +W+SKQEYDE G Sbjct: 383 TASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 86.6 bits (205), Expect = 3e-18 Identities = 45/108 (41%), Positives = 68/108 (62%), Gaps = 6/108 (5%) Frame = -2 Query: 575 GIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 396 GI + N I +CDV+IR L GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 395 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 270 ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 73.3 bits (172), Expect = 3e-14 Identities = 45/118 (38%), Positives = 69/118 (58%), Gaps = 12/118 (10%) Frame = -2 Query: 611 FLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITAL 432 F P LV G E G+ E +++I DV+IRK LY GG++MY G+ R++KEI L Sbjct: 262 FQPHLV-GSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQL 320 Query: 431 --------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 294 P+ + K+KI P R+++V+IGG++LA ++ MW+SK E++E G Sbjct: 321 WFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 52.0 bits (119), Expect = 7e-08 Identities = 26/118 (22%), Positives = 54/118 (45%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++IR+P+ + G + GI E + + + + ++ L + GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 282 +++E+T++ P I + W G S + F+ ++++EY E GP + Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 48.8 bits (111), Expect = 7e-07 Identities = 27/111 (24%), Positives = 51/111 (45%), Gaps = 14/111 (12%) Frame = -2 Query: 566 ETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAPSTMK--------- 414 E N + +++RK LY GG+T++ +R+Q+++ + + Sbjct: 309 ELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAK 368 Query: 413 -----IKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 + +I+ ++ +VW GGS+LA F +K +Y+E G SI R Sbjct: 369 SGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 48.4 bits (110), Expect = 9e-07 Identities = 29/101 (28%), Positives = 46/101 (45%) Frame = -2 Query: 575 GIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 396 GI E S+ NI+ L GG +PG R+ E+ +LAP+ ++K+ P Sbjct: 299 GIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEP 358 Query: 395 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK 273 + W S + L + I++ EY E G +I+ RK Sbjct: 359 SDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 42.3 bits (95), Expect = 6e-05 Identities = 23/96 (23%), Positives = 44/96 (45%), Gaps = 7/96 (7%) Frame = -2 Query: 539 CDVNIR----KDLYATPYCPGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKY 381 CD N+ K+LY++ GG +PG A +++ I + + I +I PP + ++ Sbjct: 517 CDGNLSDEKAKNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQF 576 Query: 380 SVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK 273 W G I + ++WI ++ G ++ K Sbjct: 577 VAWKGACIYNRIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = -2 Query: 374 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 273 ++GGSI+A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1284 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +3 Query: 315 FRDPHLLEGREGGEDRSTDPYGVLSLWRS 401 F HLL + G+D + + YG LS W S Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSWIS 825 >SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces pombe|chr 1|||Manual Length = 343 Score = 26.6 bits (56), Expect = 3.0 Identities = 12/41 (29%), Positives = 24/41 (58%), Gaps = 2/41 (4%) Frame = -2 Query: 614 GFLPTLVFGYEACGIHETTYNSIMKCD--VNIRKDLYATPY 498 G++ LV GYE+ + + Y+++ +C+ + R L +T Y Sbjct: 11 GYIEALVRGYESALLEQHIYSNLSQCESLEDFRLQLSSTDY 51 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 26.6 bits (56), Expect = 3.0 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +3 Query: 468 SRVHGGTTRTIRCCVQVLTDVHVALH 545 +R+ R RCC Q L D+H +H Sbjct: 74 NRLEEAFCRDFRCCGQTLEDLHQLIH 99 >SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.8 bits (54), Expect = 5.3 Identities = 18/65 (27%), Positives = 31/65 (47%) Frame = -1 Query: 501 VLSGWYHHVPWNRRPYAKGNHSSRPIDNED*DHCSSREKVLRMDRWIDPRLPLYLPTDVD 322 VL WY PW + KG+ + +E + SSR + + W+D YL +++D Sbjct: 480 VLGRWYE--PW----FVKGDLN---YTSEVKERTSSRLSAVGSENWVDDDFYEYLLSEID 530 Query: 321 LETGV 307 + + V Sbjct: 531 MVSAV 535 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.8 bits (54), Expect = 5.3 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -3 Query: 382 TPYGSVDRSSPPSLPSNRCGSRNRSTTSLAPP 287 TP + PPS+PS+R R S ++ APP Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 25.8 bits (54), Expect = 5.3 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -2 Query: 449 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 339 KEI++ A S + + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC23G7.10c |||NADH-dependent flavin oxidoreductase |Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 25.4 bits (53), Expect = 7.0 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +3 Query: 477 HGGT-TRTIRCCVQVLTDVHVALHDGV-ICGLVDAASFIPKNQGWK 608 +GGT + I ++V+ V A+ D + + V A ++PK QGW+ Sbjct: 230 YGGTFEKRILFPMEVVHSVRKAIPDSMPLFYRVTATDWLPKGQGWE 275 >SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.0 bits (52), Expect = 9.2 Identities = 8/15 (53%), Positives = 13/15 (86%) Frame = +1 Query: 565 SWMPQASYPKTRVGR 609 +W+ +ASY KT++GR Sbjct: 298 TWLSKASYSKTKLGR 312 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,665,972 Number of Sequences: 5004 Number of extensions: 53539 Number of successful extensions: 177 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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