BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0251 (639 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.) 215 3e-56 SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.) 212 2e-55 SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.) 212 2e-55 SB_56| Best HMM Match : Actin (HMM E-Value=0) 212 2e-55 SB_56628| Best HMM Match : Actin (HMM E-Value=0) 212 2e-55 SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.) 210 7e-55 SB_13344| Best HMM Match : Actin (HMM E-Value=1.5e-07) 185 3e-47 SB_26136| Best HMM Match : Actin (HMM E-Value=7.2e-10) 63 2e-10 SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07) 62 3e-10 SB_19204| Best HMM Match : No HMM Matches (HMM E-Value=.) 53 2e-07 SB_22343| Best HMM Match : No HMM Matches (HMM E-Value=.) 43 2e-04 SB_54| Best HMM Match : Actin (HMM E-Value=0) 36 0.021 SB_38989| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.2 SB_5589| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7 SB_665| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7 >SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 349 Score = 215 bits (524), Expect = 3e-56 Identities = 99/123 (80%), Positives = 107/123 (86%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ L G E+ GIHETTYNSIMKCDV+IRKDLYA GGTTMYPG+ADR Sbjct: 227 ERFRCPEALLQPSFLGMESSGIHETTYNSIMKCDVDIRKDLYANTVMSGGTTMYPGLADR 286 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEI+ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP+IVHR Sbjct: 287 MQKEISALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPAIVHR 346 Query: 275 KCF 267 KCF Sbjct: 347 KCF 349 Score = 31.5 bits (68), Expect = 0.60 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMK 581 N+RF CP+A QP FLGM+ Sbjct: 226 NERFRCPEALLQPSFLGME 244 >SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 338 Score = 212 bits (518), Expect = 2e-55 Identities = 98/123 (79%), Positives = 105/123 (85%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G E+ GIHETTYNSIMKCDV+IRKDLYA GGTTMYPGIADR Sbjct: 216 ERFRCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 275 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEI+ALAP TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP+IVHR Sbjct: 276 MQKEISALAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPAIVHR 335 Query: 275 KCF 267 KCF Sbjct: 336 KCF 338 Score = 34.7 bits (76), Expect = 0.064 Identities = 14/21 (66%), Positives = 17/21 (80%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMKLA 575 N+RF CP+A FQP FLGM+ A Sbjct: 215 NERFRCPEAMFQPSFLGMESA 235 >SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 376 Score = 212 bits (518), Expect = 2e-55 Identities = 98/123 (79%), Positives = 105/123 (85%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G E+ GIHETTYNSIMKCDV+IRKDLYA GG+TMYPGIADR Sbjct: 254 ERFRCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADR 313 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEIT+LAP TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR Sbjct: 314 MQKEITSLAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 373 Query: 275 KCF 267 KCF Sbjct: 374 KCF 376 Score = 34.7 bits (76), Expect = 0.064 Identities = 14/21 (66%), Positives = 17/21 (80%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMKLA 575 N+RF CP+A FQP FLGM+ A Sbjct: 253 NERFRCPEAMFQPSFLGMESA 273 >SB_56| Best HMM Match : Actin (HMM E-Value=0) Length = 375 Score = 212 bits (518), Expect = 2e-55 Identities = 98/123 (79%), Positives = 105/123 (85%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G E+ GIHETTYNSIMKCDV+IRKDLYA GG+TMYPGIADR Sbjct: 253 ERFRCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADR 312 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEIT+LAP TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR Sbjct: 313 MQKEITSLAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 372 Query: 275 KCF 267 KCF Sbjct: 373 KCF 375 Score = 34.7 bits (76), Expect = 0.064 Identities = 14/21 (66%), Positives = 17/21 (80%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMKLA 575 N+RF CP+A FQP FLGM+ A Sbjct: 252 NERFRCPEAMFQPSFLGMESA 272 >SB_56628| Best HMM Match : Actin (HMM E-Value=0) Length = 376 Score = 212 bits (517), Expect = 2e-55 Identities = 98/123 (79%), Positives = 105/123 (85%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G E+ GIHETTYNSIMKCDV+IRKDLYA GG+TMYPGIADR Sbjct: 254 ERFRCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADR 313 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEI+ALAP TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR Sbjct: 314 MQKEISALAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 373 Query: 275 KCF 267 KCF Sbjct: 374 KCF 376 Score = 34.7 bits (76), Expect = 0.064 Identities = 14/21 (66%), Positives = 17/21 (80%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMKLA 575 N+RF CP+A FQP FLGM+ A Sbjct: 253 NERFRCPEAMFQPSFLGMESA 273 >SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 375 Score = 210 bits (513), Expect = 7e-55 Identities = 97/123 (78%), Positives = 105/123 (85%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G E+ GIHETTYNSIMKCDV+IRKDLYA GG+TM+PGIADR Sbjct: 253 ERFRCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADR 312 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEI+ALAP TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR Sbjct: 313 MQKEISALAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 372 Query: 275 KCF 267 KCF Sbjct: 373 KCF 375 Score = 34.7 bits (76), Expect = 0.064 Identities = 14/21 (66%), Positives = 17/21 (80%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMKLA 575 N+RF CP+A FQP FLGM+ A Sbjct: 252 NERFRCPEAMFQPSFLGMESA 272 >SB_13344| Best HMM Match : Actin (HMM E-Value=1.5e-07) Length = 149 Score = 185 bits (450), Expect = 3e-47 Identities = 84/123 (68%), Positives = 97/123 (78%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ R P+ G EA GIHE YN IMKCDV+IRKDLY+ GG+TM+PGIADR Sbjct: 27 ERFRCPEAMFQPAFLGMEAPGIHEAIYNCIMKCDVDIRKDLYSNCVLSGGSTMFPGIADR 86 Query: 455 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 276 MQKEI LA ++MK+K+IAPPERKYSVWIGGSILASLSTFQQMWI+K+EY E GP IVHR Sbjct: 87 MQKEIAMLANASMKVKVIAPPERKYSVWIGGSILASLSTFQQMWIAKEEYHEYGPPIVHR 146 Query: 275 KCF 267 KCF Sbjct: 147 KCF 149 Score = 33.9 bits (74), Expect = 0.11 Identities = 13/19 (68%), Positives = 16/19 (84%) Frame = -3 Query: 637 NKRFGCPKAFFQPWFLGMK 581 N+RF CP+A FQP FLGM+ Sbjct: 26 NERFRCPEAMFQPAFLGME 44 >SB_26136| Best HMM Match : Actin (HMM E-Value=7.2e-10) Length = 543 Score = 62.9 bits (146), Expect = 2e-10 Identities = 37/122 (30%), Positives = 60/122 (49%), Gaps = 16/122 (13%) Frame = -2 Query: 572 IHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITA-------------- 435 + E N I C +++R+ LY GG+TM+ R+Q++I Sbjct: 223 LSEVVDNVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDIKRTVDARLKMSETLSG 282 Query: 434 --LAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF*T 261 + P ++ ++I+ ++Y+VW GGS+LAS F + +K +YDE GPSI F Sbjct: 283 GRIKPKPIETQVISHHMQRYAVWFGGSMLASTPEFYSVCHTKADYDEHGPSICRHNPF-L 341 Query: 260 HR 255 HR Sbjct: 342 HR 343 >SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07) Length = 240 Score = 62.5 bits (145), Expect = 3e-10 Identities = 29/85 (34%), Positives = 51/85 (60%), Gaps = 3/85 (3%) Frame = -2 Query: 590 GYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAPSTMKI 411 G A G+ + S+ D++IR L+ + GG T+ G +R+ +E+ + P +M++ Sbjct: 146 GSTAMGVTQVVTTSVGMTDIDIRAGLFNSVIVTGGNTLLQGFVERLNRELVSKTPPSMRL 205 Query: 410 KIIA---PPERKYSVWIGGSILASL 345 K+I+ E++++ WIGGSILASL Sbjct: 206 KLISNNSSVEKRFNPWIGGSILASL 230 >SB_19204| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 411 Score = 53.2 bits (122), Expect = 2e-07 Identities = 39/143 (27%), Positives = 65/143 (45%), Gaps = 29/143 (20%) Frame = -2 Query: 635 QKIRLPKGFLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLYATPYCPGGTTMYPGIADR 456 ++ P+ + E G+ E +N+I D++ R + Y GG+TMYPG+ R Sbjct: 257 ERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSR 316 Query: 455 MQKEITAL---------------------APST-----MKIKI-IAPP-ERKYSVWIGGS 360 +++EI L P T K KI I P RK+ V++GG+ Sbjct: 317 LEREIKQLYLERVLKGDTSKLSSGMGMEQIPLTADYLLQKFKIRIEDPPRRKHMVFMGGA 376 Query: 359 ILAS-LSTFQQMWISKQEYDESG 294 +LA + W++++EY+E G Sbjct: 377 VLADIMKDKDSFWMTRKEYEEKG 399 >SB_22343| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 245 Score = 42.7 bits (96), Expect = 2e-04 Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 9/94 (9%) Frame = -2 Query: 554 NSIMKCDVNIRKDLYATPYCPGGTTMYPGIADRMQKEITALAPS--------TMKIKIIA 399 +S++ C ++ RK L GGT M PG R+ +EI L S +K+ Sbjct: 64 DSLLLCPIDTRKTLAENIVLIGGTAMTPGFKHRLMQEIYLLLQSPKYKDKLFIKTVKMHQ 123 Query: 398 PP-ERKYSVWIGGSILASLSTFQQMWISKQEYDE 300 PP + W+GG+I SL +++ Y + Sbjct: 124 PPVNANITAWLGGAIFGSLEVLADRSTTRERYQQ 157 >SB_54| Best HMM Match : Actin (HMM E-Value=0) Length = 2486 Score = 36.3 bits (80), Expect = 0.021 Identities = 15/34 (44%), Positives = 25/34 (73%) Frame = -2 Query: 611 FLPTLVFGYEACGIHETTYNSIMKCDVNIRKDLY 510 F P+L+ G + GIHE+ + SI KCD+++R +L+ Sbjct: 2307 FQPSLL-GRDIDGIHESIFKSIKKCDIDLRAELF 2339 >SB_38989| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 104 Score = 28.7 bits (61), Expect = 4.2 Identities = 14/40 (35%), Positives = 20/40 (50%) Frame = -1 Query: 552 LHHEVRREHP*GLVRNTVLSGWYHHVPWNRRPYAKGNHSS 433 +HH E P N V +YH W++ PY +G +SS Sbjct: 3 VHHSRTPEEPRPASDNLVNYVYYH---WSKHPYVRGGYSS 39 >SB_5589| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 583 Score = 27.5 bits (58), Expect = 9.7 Identities = 14/40 (35%), Positives = 19/40 (47%) Frame = -3 Query: 385 STPYGSVDRSSPPSLPSNRCGSRNRSTTSLAPPLYTGSAS 266 STP +V PP PSN + ++ S +P T S S Sbjct: 217 STPQATVQTPPPPPEPSNEPSTSSKPKASPSPSSITTSTS 256 >SB_665| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 314 Score = 27.5 bits (58), Expect = 9.7 Identities = 14/46 (30%), Positives = 24/46 (52%) Frame = +1 Query: 247 QRRRCV*KHFLCTMEGPDSSYSCFEIHICWKVEREARIDPPIHTEY 384 +++RC + F CT+E P + + + CWKV PI T++ Sbjct: 23 KKQRCYDRDFCCTVERP-GLLTIPDDYTCWKVGPMWPFWSPIPTQF 67 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,959,441 Number of Sequences: 59808 Number of extensions: 464216 Number of successful extensions: 1988 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 1893 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1985 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1608851125 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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