BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0248 (656 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 160 2e-40 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 156 3e-39 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 82 8e-17 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 60 4e-10 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.021 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.048 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.2 SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 7.3 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 160 bits (388), Expect = 2e-40 Identities = 73/84 (86%), Positives = 77/84 (91%) Frame = +1 Query: 256 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGAL 435 LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+L Sbjct: 193 LTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSL 252 Query: 436 NVDLTEFQTNLVPYPRIHFPLVTY 507 NVDL EFQTNLVPYPRIHFPLVTY Sbjct: 253 NVDLNEFQTNLVPYPRIHFPLVTY 276 Score = 122 bits (294), Expect = 5e-29 Identities = 52/83 (62%), Positives = 68/83 (81%) Frame = +2 Query: 2 ARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGK 181 ARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGK Sbjct: 108 ARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGK 167 Query: 182 KSKLEFAIYPAPQVSTAVVEPYN 250 KS L+F++YPAPQVST+VVEPYN Sbjct: 168 KSNLQFSVYPAPQVSTSVVEPYN 190 Score = 79.8 bits (188), Expect = 3e-16 Identities = 38/64 (59%), Positives = 43/64 (67%), Gaps = 3/64 (4%) Frame = +3 Query: 474 LPPYP---LPTGHVRPVISAEKAYHEQLSVAEITNACFEPANQMVKSDPRHGKYMGCCML 644 L PYP P P++SA KA+HE SV EITN CFEP NQMVK DPR G+YM C+L Sbjct: 263 LVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLL 322 Query: 645 DRGD 656 RGD Sbjct: 323 YRGD 326 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 156 bits (378), Expect = 3e-39 Identities = 70/84 (83%), Positives = 77/84 (91%) Frame = +1 Query: 256 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGAL 435 LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+L Sbjct: 189 LTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSL 248 Query: 436 NVDLTEFQTNLVPYPRIHFPLVTY 507 NVDL EFQTNLVPYPRIHFPLVTY Sbjct: 249 NVDLAEFQTNLVPYPRIHFPLVTY 272 Score = 120 bits (288), Expect = 2e-28 Identities = 52/83 (62%), Positives = 67/83 (80%) Frame = +2 Query: 2 ARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGK 181 ARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ERL+++Y K Sbjct: 104 ARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTK 163 Query: 182 KSKLEFAIYPAPQVSTAVVEPYN 250 KSKL+F++YPAPQVST+VVEPYN Sbjct: 164 KSKLQFSVYPAPQVSTSVVEPYN 186 Score = 79.8 bits (188), Expect = 3e-16 Identities = 38/64 (59%), Positives = 43/64 (67%), Gaps = 3/64 (4%) Frame = +3 Query: 474 LPPYP---LPTGHVRPVISAEKAYHEQLSVAEITNACFEPANQMVKSDPRHGKYMGCCML 644 L PYP P P++SA KA+HE SV EITN CFEP NQMVK DPR G+YM C+L Sbjct: 259 LVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLL 318 Query: 645 DRGD 656 RGD Sbjct: 319 YRGD 322 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 81.8 bits (193), Expect = 8e-17 Identities = 35/84 (41%), Positives = 54/84 (64%) Frame = +1 Query: 256 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGAL 435 L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G L Sbjct: 187 LSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGEL 246 Query: 436 NVDLTEFQTNLVPYPRIHFPLVTY 507 N DL + N+VP+PR+HF +V + Sbjct: 247 NSDLRKLAVNMVPFPRLHFFMVGF 270 Score = 71.7 bits (168), Expect = 8e-14 Identities = 33/83 (39%), Positives = 47/83 (56%) Frame = +2 Query: 2 ARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGK 181 A+GHYT G E+ D VLD +R+ A+ C LQGF + H LL+ ++ +Y Sbjct: 102 AKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPD 161 Query: 182 KSKLEFAIYPAPQVSTAVVEPYN 250 + F++ PAP+ S VVEPYN Sbjct: 162 RMMATFSVAPAPKSSDTVVEPYN 184 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/48 (37%), Positives = 28/48 (58%) Frame = +3 Query: 510 PVISAEKAYHEQLSVAEITNACFEPANQMVKSDPRHGKYMGCCMLDRG 653 P+ + + + +SV E+T F+ N MV +DPRHG+Y+ L RG Sbjct: 272 PLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRG 319 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 59.7 bits (138), Expect = 4e-10 Identities = 26/76 (34%), Positives = 44/76 (57%) Frame = +1 Query: 280 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQ 459 ++D ++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSII 257 Query: 460 TNLVPYPRIHFPLVTY 507 +L+P PR HF L +Y Sbjct: 258 ASLIPSPRCHFLLTSY 273 Score = 46.8 bits (106), Expect = 3e-06 Identities = 26/80 (32%), Positives = 41/80 (51%), Gaps = 1/80 (1%) Frame = +2 Query: 14 YTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKL 193 Y+ + I + ++D I + AD L+GF + H L+ERL+ Y KK Sbjct: 108 YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQ 167 Query: 194 EFAIYPAPQ-VSTAVVEPYN 250 ++++P Q VS VV+PYN Sbjct: 168 TYSVFPNSQSVSDVVVQPYN 187 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 33.9 bits (74), Expect = 0.021 Identities = 18/54 (33%), Positives = 30/54 (55%) Frame = -3 Query: 474 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 313 R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 32.7 bits (71), Expect = 0.048 Identities = 18/42 (42%), Positives = 24/42 (57%) Frame = +1 Query: 469 VPYPRIHFPLVTYGQSSLPRRPTMNSFPSPRSQTHASSPPTR 594 VP P P V+ +SS+P P N+ PSP S + AS+ P R Sbjct: 1239 VPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.2 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +1 Query: 511 QSSLPRRPTMNSFPSPRSQTHASSPPTR 594 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.4 bits (53), Expect = 7.3 Identities = 15/58 (25%), Positives = 27/58 (46%) Frame = -3 Query: 468 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 295 Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,762,426 Number of Sequences: 5004 Number of extensions: 55557 Number of successful extensions: 179 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 179 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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