BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0242 (715 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 175 7e-45 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 99 3e-22 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 94 2e-20 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 92 6e-20 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 3e-11 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 60 3e-10 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 50 3e-07 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 35 0.013 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.094 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.50 SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 27 2.0 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 2.7 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 3.5 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.7 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 4.7 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 175 bits (425), Expect = 7e-45 Identities = 78/84 (92%), Positives = 80/84 (95%) Frame = +1 Query: 256 GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKA 435 GDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+ Sbjct: 55 GDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKS 114 Query: 436 NREKMTQIMFETFNTPAMYVAIQA 507 NREKMTQI+FETFN PA YVAIQA Sbjct: 115 NREKMTQIIFETFNAPAFYVAIQA 138 Score = 109 bits (261), Expect = 5e-25 Identities = 47/52 (90%), Positives = 51/52 (98%) Frame = +2 Query: 98 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSY 253 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSY Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSY 53 Score = 93.5 bits (222), Expect = 3e-20 Identities = 43/45 (95%), Positives = 44/45 (97%) Frame = +3 Query: 510 LSLYASGRTTGIVLDSGDGVFHTVPIYEGYALPHAILRLDLAGRD 644 LSLYASGRTTGIVLDSGDGV HTVPIYEGYALPHAI+RLDLAGRD Sbjct: 140 LSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRD 184 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 99 bits (238), Expect = 3e-22 Identities = 41/96 (42%), Positives = 68/96 (70%) Frame = +1 Query: 220 RDGRYGTEGLL*GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 399 + G + ++ GDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 400 VLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQA 507 +LLTE P+NP ANREKM + MFE + +YVAIQA Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQA 138 Score = 65.3 bits (152), Expect = 8e-12 Identities = 29/48 (60%), Positives = 36/48 (75%) Frame = +3 Query: 510 LSLYASGRTTGIVLDSGDGVFHTVPIYEGYALPHAILRLDLAGRDPHR 653 LSLYA G ++G+V+DSGDGV H VP+YE L H + RLD+AGRD R Sbjct: 140 LSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATR 187 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +2 Query: 104 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 208 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 94.3 bits (224), Expect = 2e-20 Identities = 41/85 (48%), Positives = 54/85 (63%), Gaps = 1/85 (1%) Frame = +1 Query: 256 GDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPK 432 G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP Sbjct: 77 GNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPP 136 Query: 433 ANREKMTQIMFETFNTPAMYVAIQA 507 NRE +IMFE+FN +Y+A+QA Sbjct: 137 ENRENTAEIMFESFNCAGLYIAVQA 161 Score = 47.6 bits (108), Expect = 2e-06 Identities = 20/36 (55%), Positives = 26/36 (72%) Frame = +3 Query: 537 TGIVLDSGDGVFHTVPIYEGYALPHAILRLDLAGRD 644 TG V+DSGDGV H +P+ EGY + +I + LAGRD Sbjct: 180 TGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRD 215 Score = 41.9 bits (94), Expect = 9e-05 Identities = 20/48 (41%), Positives = 30/48 (62%), Gaps = 2/48 (4%) Frame = +2 Query: 116 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSY 253 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSY 54 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 92.3 bits (219), Expect = 6e-20 Identities = 40/84 (47%), Positives = 58/84 (69%), Gaps = 1/84 (1%) Frame = +1 Query: 256 GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPK 432 G EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL Sbjct: 59 GSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANI 118 Query: 433 ANREKMTQIMFETFNTPAMYVAIQ 504 N+EK+ + +ET N PA+ ++Q Sbjct: 119 RNKEKIAEYFYETLNVPALSFSLQ 142 Score = 64.9 bits (151), Expect = 1e-11 Identities = 27/45 (60%), Positives = 38/45 (84%) Frame = +3 Query: 510 LSLYASGRTTGIVLDSGDGVFHTVPIYEGYALPHAILRLDLAGRD 644 L+LYAS RTTGIVL+ GDG+ H+VPIY+G+++P AI + ++ GRD Sbjct: 145 LALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRD 189 Score = 59.7 bits (138), Expect = 4e-10 Identities = 25/46 (54%), Positives = 33/46 (71%) Frame = +2 Query: 116 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSY 253 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMF 57 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 63.3 bits (147), Expect = 3e-11 Identities = 29/73 (39%), Positives = 41/73 (56%) Frame = +1 Query: 286 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 465 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 466 ETFNTPAMYVAIQ 504 E+ PA Y+A Q Sbjct: 123 ESLRCPATYLAKQ 135 Score = 40.3 bits (90), Expect = 3e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 101 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 202 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 60.1 bits (139), Expect = 3e-10 Identities = 30/95 (31%), Positives = 53/95 (55%), Gaps = 1/95 (1%) Frame = +1 Query: 220 RDGRYGTEGLL*GDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 396 RD + L G++ + G ++ + P E +++NWD ME++ +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 Query: 397 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAI 501 P+ +TE NP R MT+++FE +N P++ I Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151 Score = 35.9 bits (79), Expect = 0.006 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 116 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 211 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 50.0 bits (114), Expect = 3e-07 Identities = 20/66 (30%), Positives = 38/66 (57%) Frame = +1 Query: 310 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 489 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 490 YVAIQA 507 Y+ QA Sbjct: 134 YLTKQA 139 Score = 36.7 bits (81), Expect = 0.003 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +2 Query: 101 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 202 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 34.7 bits (76), Expect = 0.013 Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%) Frame = +1 Query: 301 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 468 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 469 TFNTPAMYVAIQ 504 P +A + Sbjct: 172 ECQVPGFTIAYE 183 Score = 28.3 bits (60), Expect = 1.2 Identities = 16/44 (36%), Positives = 22/44 (50%) Frame = +3 Query: 510 LSLYASGRTTGIVLDSGDGVFHTVPIYEGYALPHAILRLDLAGR 641 + LYA G G+V+D G PI +G + A +L L GR Sbjct: 186 MGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGR 229 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.094 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 361 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 504 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 29.5 bits (63), Expect = 0.50 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -2 Query: 333 IPVSDDSVFDGVFEGQDTSFALCLISS*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 154 +P + +V +TSFAL S S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 153 PALHIP 136 P L+ P Sbjct: 123 PQLNSP 128 >SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 658 Score = 27.5 bits (58), Expect = 2.0 Identities = 29/94 (30%), Positives = 37/94 (39%), Gaps = 7/94 (7%) Frame = +1 Query: 442 EKMTQIMFETFNTPAMYVAIQAGS--RCTRP----VVPPVSCWT-PATVSSTPCPSTRDT 600 E M M E TP V S R T P + P V T P +TP T T Sbjct: 220 ETMITPMVEAMTTPTEVVGRSMVSTIRTTTPMEAMITPTVETTTLPTAAMTTPVEETTTT 279 Query: 601 HFPTPFCVWTWPVVTLTDYLMKILTRARVLVTTP 702 P + T VVT T +++ + V+ TTP Sbjct: 280 --PMVETMITPTVVTTTTPMVETMITPTVVTTTP 311 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 27.1 bits (57), Expect = 2.7 Identities = 10/33 (30%), Positives = 19/33 (57%) Frame = +3 Query: 543 IVLDSGDGVFHTVPIYEGYALPHAILRLDLAGR 641 +V+DSG H +P+ + A+ R+D+ G+ Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGK 191 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 3.5 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -2 Query: 231 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 97 PT+ P P N S P PL+TTN TS+S Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 403 LLTEAPLNPKANREKMTQIMFETFN 477 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -2 Query: 237 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 145 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.4 bits (53), Expect = 8.1 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +1 Query: 553 TPATVSST-PCPSTRDTHFPTPFCVWTWPVVTLTDYL 660 T +++ +T P ST + T + W+ PVV +TDYL Sbjct: 377 TNSSIPTTYPSNSTTYQNITTSY-PWSQPVVNITDYL 412 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,153,889 Number of Sequences: 5004 Number of extensions: 70775 Number of successful extensions: 239 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 203 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 237 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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