BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0222 (684 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 123 2e-29 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 81 1e-16 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 80 3e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 76 4e-15 SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 32 0.067 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.4 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 27 1.9 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.5 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.3 SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.4 SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 26 5.8 SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce... 25 7.7 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 25 7.7 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.7 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 123 bits (297), Expect = 2e-29 Identities = 52/67 (77%), Positives = 61/67 (91%) Frame = +1 Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432 LEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+C Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127 Query: 433 DCLQGFQ 453 D LQGFQ Sbjct: 128 DALQGFQ 134 Score = 120 bits (290), Expect = 1e-28 Identities = 50/70 (71%), Positives = 59/70 (84%) Frame = +2 Query: 53 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 232 MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 233 PRAILVDWSP 262 PRA+LVD P Sbjct: 61 PRAVLVDLEP 70 Score = 81.8 bits (193), Expect = 8e-17 Identities = 36/45 (80%), Positives = 42/45 (93%) Frame = +3 Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDK 644 KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+D+ Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDE 198 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 81.0 bits (191), Expect = 1e-16 Identities = 32/66 (48%), Positives = 48/66 (72%) Frame = +1 Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432 LEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133 Query: 433 DCLQGF 450 LQGF Sbjct: 134 SGLQGF 139 Score = 56.0 bits (129), Expect = 5e-09 Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 6/76 (7%) Frame = +2 Query: 53 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 214 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 215 SGGKYVPRAILVDWSP 262 GK+VPR+I VD P Sbjct: 61 GQGKFVPRSIYVDLEP 76 Score = 44.4 bits (100), Expect = 2e-05 Identities = 18/44 (40%), Positives = 30/44 (68%) Frame = +3 Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTD 641 ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSD 203 Score = 26.6 bits (56), Expect = 3.3 Identities = 9/13 (69%), Positives = 11/13 (84%) Frame = +2 Query: 644 TYCIDNEALYDIC 682 T+ +DNEA YDIC Sbjct: 205 TFMVDNEACYDIC 217 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 79.8 bits (188), Expect = 3e-16 Identities = 32/66 (48%), Positives = 46/66 (69%) Frame = +1 Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432 LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129 Query: 433 DCLQGF 450 LQGF Sbjct: 130 SGLQGF 135 Score = 62.1 bits (144), Expect = 7e-11 Identities = 30/72 (41%), Positives = 41/72 (56%), Gaps = 2/72 (2%) Frame = +2 Query: 53 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 226 MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 227 YVPRAILVDWSP 262 YVPR+I VD P Sbjct: 61 YVPRSIYVDLEP 72 Score = 40.7 bits (91), Expect = 2e-04 Identities = 17/44 (38%), Positives = 28/44 (63%) Frame = +3 Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTD 641 ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLAD 199 Score = 25.4 bits (53), Expect = 7.7 Identities = 8/13 (61%), Positives = 11/13 (84%) Frame = +2 Query: 644 TYCIDNEALYDIC 682 T+ +DNE+ YDIC Sbjct: 201 TFMVDNESCYDIC 213 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 76.2 bits (179), Expect = 4e-15 Identities = 32/69 (46%), Positives = 48/69 (69%) Frame = +2 Query: 56 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 235 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 236 RAILVDWSP 262 RAIL+D P Sbjct: 63 RAILIDLEP 71 Score = 56.4 bits (130), Expect = 4e-09 Identities = 22/68 (32%), Positives = 44/68 (64%), Gaps = 2/68 (2%) Frame = +1 Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKESE 426 LEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E++ Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127 Query: 427 SCDCLQGF 450 D L+GF Sbjct: 128 GSDSLEGF 135 Score = 48.0 bits (109), Expect = 1e-06 Identities = 22/50 (44%), Positives = 32/50 (64%), Gaps = 1/50 (2%) Frame = +3 Query: 510 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDKPTAL 656 ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D L Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVL 205 >SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 967 Score = 32.3 bits (70), Expect = 0.067 Identities = 18/43 (41%), Positives = 24/43 (55%) Frame = +1 Query: 220 RQVRAPRHSRRLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 348 R+V AP+H+RR GT RS +FR ++ FGQ GN Sbjct: 486 REVFAPKHARR-RLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 27.9 bits (59), Expect = 1.4 Identities = 16/41 (39%), Positives = 23/41 (56%) Frame = +2 Query: 452 NLHIPSVAAPGPVWAPSSSKDP*RVPRQNHEHILSSPLAQS 574 N+H PS A + ++P +P+QNHEH SPL +S Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 27.5 bits (58), Expect = 1.9 Identities = 25/96 (26%), Positives = 31/96 (32%) Frame = -2 Query: 344 PAPDCPKTKLSGRKICPKGPERTESMVPGSSRREWRGARTCRRRLHCSIH*CAPTASQSP 165 P P CP +K C G E+ R R C + L C H C Sbjct: 667 PCPPCPYFV---KKRCLCGKHILENQP--CYRENVRCGELCNKLLSCKTHFCEKLCHPDG 721 Query: 164 HGRHRWGRCRARQR*SPRT*LQSGYHIGRPVDERFP 57 + ++R QS H G P DER P Sbjct: 722 ECESSCKKECGKRRMYCEHVCQSPCHAGHPCDERIP 757 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +1 Query: 355 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 444 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 2.5 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 92 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 193 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 3.3 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 286 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 387 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 26.2 bits (55), Expect = 4.4 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +3 Query: 36 KITYTK*GKSFIYRPANVV 92 +I+YT G SFIYRP N++ Sbjct: 64 EISYT--GTSFIYRPGNII 80 >SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharomyces pombe|chr 2|||Manual Length = 883 Score = 25.8 bits (54), Expect = 5.8 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = -1 Query: 339 AGLSEDEVVRTEDLSERSGADRVHGAGLQSTRMARG 232 AG++ED + T + + +G++R L+ +R+ G Sbjct: 279 AGINEDHTLATLSIIDLAGSERATATKLRGSRLFEG 314 >SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 25.4 bits (53), Expect = 7.7 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +2 Query: 467 SVAAPGPVWAPSSSKDP*RVPRQNHEHILSSPLAQS 574 S A P +PS+SK P R+N + SP+A + Sbjct: 168 SAGAAAPTPSPSNSKSPPSENRKNRLQNVLSPIASN 203 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 25.4 bits (53), Expect = 7.7 Identities = 14/46 (30%), Positives = 24/46 (52%) Frame = +2 Query: 485 PVWAPSSSKDP*RVPRQNHEHILSSPLAQSIRHCRRTIQCDSLSSP 622 P WA + +VP++NH H + S L+ + ++ + LSSP Sbjct: 628 PFWAIQPKQSSSQVPKENH-HNIQSKLSIN-TEAATDLKANELSSP 671 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 7.7 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +1 Query: 307 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 438 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,855,251 Number of Sequences: 5004 Number of extensions: 59770 Number of successful extensions: 197 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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