SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0222
         (684 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   123   2e-29
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    81   1e-16
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    80   3e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    76   4e-15
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces...    32   0.067
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    28   1.4  
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo...    27   1.9  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        27   1.9  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.5  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.3  
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha...    26   4.4  
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom...    26   5.8  
SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce...    25   7.7  
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||...    25   7.7  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    25   7.7  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  123 bits (297), Expect = 2e-29
 Identities = 52/67 (77%), Positives = 61/67 (91%)
 Frame = +1

Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432
           LEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+C
Sbjct: 68  LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127

Query: 433 DCLQGFQ 453
           D LQGFQ
Sbjct: 128 DALQGFQ 134



 Score =  120 bits (290), Expect = 1e-28
 Identities = 50/70 (71%), Positives = 59/70 (84%)
 Frame = +2

Query: 53  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 232
           MREIVH+QAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 233 PRAILVDWSP 262
           PRA+LVD  P
Sbjct: 61  PRAVLVDLEP 70



 Score = 81.8 bits (193), Expect = 8e-17
 Identities = 36/45 (80%), Positives = 42/45 (93%)
 Frame = +3

Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDK 644
           KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+D+
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDE 198


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 81.0 bits (191), Expect = 1e-16
 Identities = 32/66 (48%), Positives = 48/66 (72%)
 Frame = +1

Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432
           LEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSVL+ +R+ +++C
Sbjct: 74  LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133

Query: 433 DCLQGF 450
             LQGF
Sbjct: 134 SGLQGF 139



 Score = 56.0 bits (129), Expect = 5e-09
 Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 6/76 (7%)
 Frame = +2

Query: 53  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 214
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 215 SGGKYVPRAILVDWSP 262
             GK+VPR+I VD  P
Sbjct: 61  GQGKFVPRSIYVDLEP 76



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 18/44 (40%), Positives = 30/44 (68%)
 Frame = +3

Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTD 641
           ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++N+D
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSD 203



 Score = 26.6 bits (56), Expect = 3.3
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +2

Query: 644 TYCIDNEALYDIC 682
           T+ +DNEA YDIC
Sbjct: 205 TFMVDNEACYDIC 217


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 79.8 bits (188), Expect = 3e-16
 Identities = 32/66 (48%), Positives = 46/66 (69%)
 Frame = +1

Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432
           LEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D +R+ +++C
Sbjct: 70  LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129

Query: 433 DCLQGF 450
             LQGF
Sbjct: 130 SGLQGF 135



 Score = 62.1 bits (144), Expect = 7e-11
 Identities = 30/72 (41%), Positives = 41/72 (56%), Gaps = 2/72 (2%)
 Frame = +2

Query: 53  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 226
           MREI+ +  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 227 YVPRAILVDWSP 262
           YVPR+I VD  P
Sbjct: 61  YVPRSIYVDLEP 72



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 17/44 (38%), Positives = 28/44 (63%)
 Frame = +3

Query: 510 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTD 641
           ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++  D
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLAD 199



 Score = 25.4 bits (53), Expect = 7.7
 Identities = 8/13 (61%), Positives = 11/13 (84%)
 Frame = +2

Query: 644 TYCIDNEALYDIC 682
           T+ +DNE+ YDIC
Sbjct: 201 TFMVDNESCYDIC 213


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 76.2 bits (179), Expect = 4e-15
 Identities = 32/69 (46%), Positives = 48/69 (69%)
 Frame = +2

Query: 56  REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 235
           REI+ LQAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 236 RAILVDWSP 262
           RAIL+D  P
Sbjct: 63  RAILIDLEP 71



 Score = 56.4 bits (130), Expect = 4e-09
 Identities = 22/68 (32%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
 Frame = +1

Query: 253 LEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKESE 426
           LEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D++ +E++
Sbjct: 69  LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127

Query: 427 SCDCLQGF 450
             D L+GF
Sbjct: 128 GSDSLEGF 135



 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 22/50 (44%), Positives = 32/50 (64%), Gaps = 1/50 (2%)
 Frame = +3

Query: 510 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDKPTAL 656
           ++ + YP +I+ TYSV P S  VSD VV+PYN+ L++ +L  N D    L
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVL 205


>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 967

 Score = 32.3 bits (70), Expect = 0.067
 Identities = 18/43 (41%), Positives = 24/43 (55%)
 Frame = +1

Query: 220 RQVRAPRHSRRLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 348
           R+V AP+H+RR   GT    RS     +FR ++  FGQ   GN
Sbjct: 486 REVFAPKHARR-RLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527


>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 682

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 16/41 (39%), Positives = 23/41 (56%)
 Frame = +2

Query: 452 NLHIPSVAAPGPVWAPSSSKDP*RVPRQNHEHILSSPLAQS 574
           N+H PS        A +  ++P  +P+QNHEH   SPL +S
Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660


>SPCC18.03 |||shuttle craft like transcriptional
           regulator|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1077

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 25/96 (26%), Positives = 31/96 (32%)
 Frame = -2

Query: 344 PAPDCPKTKLSGRKICPKGPERTESMVPGSSRREWRGARTCRRRLHCSIH*CAPTASQSP 165
           P P CP      +K C  G    E+      R   R    C + L C  H C        
Sbjct: 667 PCPPCPYFV---KKRCLCGKHILENQP--CYRENVRCGELCNKLLSCKTHFCEKLCHPDG 721

Query: 164 HGRHRWGRCRARQR*SPRT*LQSGYHIGRPVDERFP 57
                  +   ++R       QS  H G P DER P
Sbjct: 722 ECESSCKKECGKRRMYCEHVCQSPCHAGHPCDERIP 757


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +1

Query: 355 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 444
           A+GH   G ELV +  D +RK+SE+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 92  NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 193
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +1

Query: 286 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 387
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 363

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +3

Query: 36  KITYTK*GKSFIYRPANVV 92
           +I+YT  G SFIYRP N++
Sbjct: 64  EISYT--GTSFIYRPGNII 80


>SPBC2F12.13 |klp5|sot1|kinesin-like protein
           Klp5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 883

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 10/36 (27%), Positives = 21/36 (58%)
 Frame = -1

Query: 339 AGLSEDEVVRTEDLSERSGADRVHGAGLQSTRMARG 232
           AG++ED  + T  + + +G++R     L+ +R+  G
Sbjct: 279 AGINEDHTLATLSIIDLAGSERATATKLRGSRLFEG 314


>SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 428

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = +2

Query: 467 SVAAPGPVWAPSSSKDP*RVPRQNHEHILSSPLAQS 574
           S  A  P  +PS+SK P    R+N    + SP+A +
Sbjct: 168 SAGAAAPTPSPSNSKSPPSENRKNRLQNVLSPIASN 203


>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1116

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 14/46 (30%), Positives = 24/46 (52%)
 Frame = +2

Query: 485 PVWAPSSSKDP*RVPRQNHEHILSSPLAQSIRHCRRTIQCDSLSSP 622
           P WA    +   +VP++NH H + S L+ +       ++ + LSSP
Sbjct: 628 PFWAIQPKQSSSQVPKENH-HNIQSKLSIN-TEAATDLKANELSSP 671


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +1

Query: 307  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 438
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,855,251
Number of Sequences: 5004
Number of extensions: 59770
Number of successful extensions: 197
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -