SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0221
         (569 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    35   0.010
SPBC146.03c |cut3|smc4, smc4|condensin subunit Cut3|Schizosaccha...    32   0.068
SPAC27D7.05c |apc14|omt1|anaphase-promoting complex subunit Apc1...    30   0.27 
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz...    29   0.63 
SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre...    29   0.63 
SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharo...    29   0.63 
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    28   0.84 
SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce...    28   0.84 
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    28   0.84 
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc...    27   1.9  
SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5 |Schizo...    27   2.6  
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||...    27   2.6  
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces...    27   2.6  
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi...    26   3.4  
SPBC1685.08 |||histone deacetylase complex subunit Cti6|Schizosa...    26   4.5  
SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|...    26   4.5  
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha...    26   4.5  
SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc...    25   5.9  
SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosacchar...    25   5.9  
SPAC22E12.06c |gmh3||alpha-1,2-galactosyltransferase Gmh3|Schizo...    25   5.9  
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce...    25   5.9  
SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces p...    25   7.8  
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po...    25   7.8  

>SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 345

 Score = 34.7 bits (76), Expect = 0.010
 Identities = 19/85 (22%), Positives = 41/85 (48%)
 Frame = -2

Query: 517 DVCVMKNAGLSGKEMPAKAKLEKEIDEIRSKLSKNDSEFPNEKNRFQTEIAKLKDVNAKL 338
           D   M+ A +S   +  K +  KE  +I+ +  +  ++   E+ + + EI +L+    + 
Sbjct: 114 DSLKMQKASVSSM-VNRKQRAAKEEQKIQEEFERQITDLLEEQQQLKLEIERLEAETERA 172

Query: 337 EGDKDVFANKYKALENETSNLSNQC 263
             + + +  + +ALE E   L N+C
Sbjct: 173 NSETEQYEKQKEALEEEYEELRNEC 197


>SPBC146.03c |cut3|smc4, smc4|condensin subunit
           Cut3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1324

 Score = 31.9 bits (69), Expect = 0.068
 Identities = 20/81 (24%), Positives = 40/81 (49%)
 Frame = -1

Query: 248 EMKNREAQINKLSADLKNATSLQTAMSDCMKKIEDLKKEIDSKGKDIE*LKNQVGNVYKN 69
           ++++ ++ ++KL  D +   ++   +S  +K + DLK E     K+IE  K  V N Y+N
Sbjct: 557 DVESSQSSLDKLRNDAEENRNI---LSSKLKVLSDLKGEKKDVSKNIERKKETVHNTYRN 613

Query: 68  RPRIRISCSKRLADKSKNKKK 6
               R    +  A  S ++ +
Sbjct: 614 LMSNRTKLEEMKASLSSSRSR 634


>SPAC27D7.05c |apc14|omt1|anaphase-promoting complex subunit
           Apc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 107

 Score = 29.9 bits (64), Expect = 0.27
 Identities = 19/62 (30%), Positives = 28/62 (45%)
 Frame = +3

Query: 327 LSPSNLALTSFSFAISVWNLFFSFGNSESFFDSLERISSISFSNLAFAGISLPDNPAFFI 506
           L+   L L   S AI  WN     G  E+  +  ER S I+ S +A +  S+P+     +
Sbjct: 32  LAKKPLLLPEASKAIEFWNYRDVIGGEEAEQERNERASRIAISRIASSRSSIPEYRQAIM 91

Query: 507 TH 512
            H
Sbjct: 92  QH 93


>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
           Mad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 28.7 bits (61), Expect = 0.63
 Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 3/63 (4%)
 Frame = -2

Query: 451 KEIDEIRSKLSKNDSEFPNEKNR---FQTEIAKLKDVNAKLEGDKDVFANKYKALENETS 281
           KEI E+++++ KND    +EKN       +  ++KD N  L   + +FA+  + LE +  
Sbjct: 144 KEIAELKTQIEKNDQAL-SEKNHEVMVSNQALQMKDTN--LTNLEKLFADSREQLETKCK 200

Query: 280 NLS 272
            L+
Sbjct: 201 ELA 203



 Score = 25.8 bits (54), Expect = 4.5
 Identities = 13/39 (33%), Positives = 20/39 (51%)
 Frame = -2

Query: 457 LEKEIDEIRSKLSKNDSEFPNEKNRFQTEIAKLKDVNAK 341
           +EK+++E  +   K   E  NEK   Q +I +L D   K
Sbjct: 106 VEKQLEEQSTSYQKEIEEVRNEKEATQVKIHELLDAKWK 144


>SPBC19C2.09 |sre1||sterol regulatory element binding protein
           Sre1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 900

 Score = 28.7 bits (61), Expect = 0.63
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = +3

Query: 312 LANTSLSPSNLALTSFSFAISVWNLFFSFGNS 407
           L NTSLSP + +L+    ++S W   F+F N+
Sbjct: 76  LTNTSLSPHSFSLSDMESSMSNWLNPFAFDNT 107


>SPAC20G8.10c ||SPAC3A12.01c|beclin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 464

 Score = 28.7 bits (61), Expect = 0.63
 Identities = 13/35 (37%), Positives = 21/35 (60%)
 Frame = -2

Query: 460 KLEKEIDEIRSKLSKNDSEFPNEKNRFQTEIAKLK 356
           KL +E+DE + K+   + EF N  N+FQ +   L+
Sbjct: 218 KLLRELDEEKEKVYAEEQEFYNNLNQFQIKKLSLE 252


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
            Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 28.3 bits (60), Expect = 0.84
 Identities = 14/46 (30%), Positives = 26/46 (56%)
 Frame = -1

Query: 203  LKNATSLQTAMSDCMKKIEDLKKEIDSKGKDIE*LKNQVGNVYKNR 66
            L+  T  +    DC++KIE+L+K+ID   +D+      +G   +N+
Sbjct: 1266 LQELTRWRDTFYDCIQKIEELEKQID-VARDVAEDTKSLGKELQNK 1310


>SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 642

 Score = 28.3 bits (60), Expect = 0.84
 Identities = 20/74 (27%), Positives = 32/74 (43%)
 Frame = -2

Query: 508 VMKNAGLSGKEMPAKAKLEKEIDEIRSKLSKNDSEFPNEKNRFQTEIAKLKDVNAKLEGD 329
           V K  G   K +   AKL K++D    K  KN +   + ++   ++  K KD +     D
Sbjct: 12  VKKKDGSMNKRVKV-AKLPKKVDSFSPKKKKNTTSSGSSESDSMSQNDKKKDSSLNESED 70

Query: 328 KDVFANKYKALENE 287
           +D       A EN+
Sbjct: 71  EDFAGFGESASEND 84


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 28.3 bits (60), Expect = 0.84
 Identities = 21/79 (26%), Positives = 41/79 (51%)
 Frame = -1

Query: 251  EEMKNREAQINKLSADLKNATSLQTAMSDCMKKIEDLKKEIDSKGKDIE*LKNQVGNVYK 72
            +++  R+ +I+ L  +  N +  Q A++    K+++   +      DIE LKN+V  V  
Sbjct: 948  KKLTARQEEISNLKEE--NMSQSQ-AITSVKSKLDETLSKSSKLEADIEHLKNKVSEVEV 1004

Query: 71   NRPRIRISCSKRLADKSKN 15
             R  + ++ ++RL D  KN
Sbjct: 1005 ERNAL-LASNERLMDDLKN 1022


>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 489

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 5/78 (6%)
 Frame = -2

Query: 505 MKNAGLSGKEMPAKAKLEKEIDEIRSKLSK-----NDSEFPNEKNRFQTEIAKLKDVNAK 341
           +K A L  +E      L K I+++ S LSK       +EF  ++      +  L   NA+
Sbjct: 391 LKAAKLPSEEENTTKWLNKAINDLVSSLSKINFSTEGTEFDKKQIEMIQNLPPLFVFNAR 450

Query: 340 LEGDKDVFANKYKALENE 287
              DK+V   + K+ EN+
Sbjct: 451 SVIDKEVVPEQEKSAENQ 468


>SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1065

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = -2

Query: 424 LSKNDSEFPNEKNRFQTEIAKLKDVNAKLEGDKDVFANKYK 302
           L K + E    KN+ Q+ +  LKD    LE + ++F  + K
Sbjct: 178 LRKREREILQNKNQGQSTLNSLKDRQQALEKEVNIFKEREK 218


>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1367

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = -2

Query: 550 NG*RFGM*NRGDVCVMKNAGLSGKE-MPAKAKLEKEIDEIRSKLSKNDSEFP 398
           NG R+G  +  D C+ K   L+  E +  K  L+K+  ++   LS +DS  P
Sbjct: 618 NGIRYGKSHNEDTCIKKRRNLNYNENLSCKVDLDKK--KMLETLSLSDSPNP 667


>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1526

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 21/81 (25%), Positives = 39/81 (48%)
 Frame = -1

Query: 248  EMKNREAQINKLSADLKNATSLQTAMSDCMKKIEDLKKEIDSKGKDIE*LKNQVGNVYKN 69
            E  N + Q+ +L A  KN   L+  + +C+  I+ L KE+++K +  + L +     Y  
Sbjct: 1096 ESLNDKDQLYQLQAT-KNK-ELEAKVKECLNNIKSLTKELENKEEKCQNL-SDASLKYIE 1152

Query: 68   RPRIRISCSKRLADKSKNKKK 6
               I  +   +++D    KKK
Sbjct: 1153 LQEIHENLLLKVSDLENYKKK 1173


>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
           Gap1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 766

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 12/44 (27%), Positives = 25/44 (56%)
 Frame = -1

Query: 245 MKNREAQINKLSADLKNATSLQTAMSDCMKKIEDLKKEIDSKGK 114
           ++NRE+     + DL   + ++ A     +++ DLK++I S+ K
Sbjct: 23  LRNRESTSVLYTIDLDMESDVEDAFFHLDRELHDLKQQISSQSK 66



 Score = 25.8 bits (54), Expect = 4.5
 Identities = 18/56 (32%), Positives = 28/56 (50%)
 Frame = +3

Query: 375 VWNLFFSFGNSESFFDSLERISSISFSNLAFAGISLPDNPAFFITHTSPLFHIPNL 542
           V NL    GN   FF++LE    I+ S  + A + +  N   ++TH   L ++ NL
Sbjct: 403 VHNLLRKLGNVGDFFEALELDQYIALSKKSLA-LEMTVN-EIYLTHEIILENLDNL 456


>SPBC1685.08 |||histone deacetylase complex subunit
           Cti6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 424

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 17/54 (31%), Positives = 28/54 (51%)
 Frame = -2

Query: 484 GKEMPAKAKLEKEIDEIRSKLSKNDSEFPNEKNRFQTEIAKLKDVNAKLEGDKD 323
           G E+  K K+E+E DEI       +S+  NE+N+ +T    + + +A  E   D
Sbjct: 199 GTEINLKQKIEEENDEILE--DSKESKDENEENK-ETSTTNVAETDAPEEETVD 249


>SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 162

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 16/37 (43%), Positives = 24/37 (64%)
 Frame = -2

Query: 502 KNAGLSGKEMPAKAKLEKEIDEIRSKLSKNDSEFPNE 392
           K + ++ KE+  K KL KE++ IRS L K  SE P++
Sbjct: 103 KQSSINDKEL--KIKL-KEVESIRSDLLKCMSEHPDK 136


>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 4196

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = +3

Query: 486  DNPAFFITHTSPLFHIPNLQPF 551
            DNP   + +T PL  I +L+PF
Sbjct: 1685 DNPICLLDNTQPLHWISSLEPF 1706


>SPBC428.03c |pho4||thiamine-repressible acid phosphatase
           Pho4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 463

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 8/21 (38%), Positives = 13/21 (61%)
 Frame = +3

Query: 477 SLPDNPAFFITHTSPLFHIPN 539
           S P+NP +F+ H +P+    N
Sbjct: 106 SYPENPLYFVKHWTPVIKAEN 126


>SPAC13F5.06c |sec10||exocyst complex subunit
           Sec10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 811

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = -1

Query: 227 QINKLSADLKNATSLQTAMSDCMKKIEDLKKEIDSKG 117
           +I K  ++L    +LQT +S   K + DLK  ++++G
Sbjct: 340 EIAKGKSNLAYLRTLQTVVSSLRKLVADLKTILENRG 376


>SPAC22E12.06c |gmh3||alpha-1,2-galactosyltransferase
           Gmh3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 332

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +3

Query: 369 ISVWNLFFSFGNSESFFDSLERISSI 446
           IS + ++F FGNS S   +LER+  +
Sbjct: 20  ISSFLIYFQFGNSFSASPALERLQDV 45


>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2104

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 9/69 (13%)
 Frame = -2

Query: 466  KAKLEKEID--EIRSKLSKNDSEFPNEKNRFQTEIAKLKDVNAKL-------EGDKDVFA 314
            KA  EK ++  E+  KL + D E   ++  + T++ +L++ NA L       E       
Sbjct: 993  KAINEKNLNIRELNEKLMRLDDELLLKQRSYDTKVQELREENASLKDQCRTYESQLASLV 1052

Query: 313  NKYKALENE 287
            +KY   E+E
Sbjct: 1053 SKYSETESE 1061


>SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1272

 Score = 25.0 bits (52), Expect = 7.8
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = +3

Query: 378 WNLFFSFGNSESFFDSLERISSISFSNLAFAGI 476
           WNL  S  NS +    +   SSISF  L   G+
Sbjct: 227 WNLDMSKSNSLNLDSIIVDTSSISFEELHITGV 259


>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 851

 Score = 25.0 bits (52), Expect = 7.8
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = -1

Query: 227 QINKLSADLKNATSLQTAMSDCMKKIEDLKKEIDSKGKDIE 105
           Q +K    +KN    Q A  +  +K++DL +E  S   D++
Sbjct: 201 QGSKAKDKIKNDPDAQKAKEETKQKLQDLYEEFKSVAVDLQ 241


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,828,893
Number of Sequences: 5004
Number of extensions: 29000
Number of successful extensions: 179
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 242064240
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -