Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0218
(615 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 75 1e-12
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 69 9e-11
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 69 9e-11
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 69 9e-11
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 69 9e-11
UniRef50_P34896 Cluster: Serine hydroxymethyltransferase, cytoso... 54 4e-06
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:... 51 2e-05
UniRef50_A4SBB9 Cluster: Serine hydroxymethyltransferase; n=11; ... 49 1e-04
UniRef50_Q5C0V4 Cluster: SJCHGC07535 protein; n=1; Schistosoma j... 46 0.001
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba... 44 0.003
UniRef50_A6MJY3 Cluster: Mitochondrial serine hydroxymethyltrans... 43 0.007
UniRef50_Q5BJF5 Cluster: Serine hydroxymethyltransferase; n=3; E... 43 0.007
UniRef50_P34897 Cluster: Serine hydroxymethyltransferase, mitoch... 43 0.007
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ... 40 0.036
UniRef50_O23984 Cluster: Expressed sequence tag; n=7; Poaceae|Re... 40 0.036
UniRef50_UPI0000E49DF3 Cluster: PREDICTED: similar to serine hyd... 40 0.062
UniRef50_Q9U638 Cluster: SHMT; n=5; Aconoidasida|Rep: SHMT - Pla... 38 0.19
UniRef50_Q8ZYF9 Cluster: Serine hydroxymethyltransferase; n=5; T... 35 1.8
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba... 34 2.3
UniRef50_Q8EWD1 Cluster: Serine hydroxymethyltransferase; n=14; ... 34 3.1
UniRef50_A7FMW0 Cluster: Opacity-associated protein A family; n=... 33 4.1
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ... 33 4.1
UniRef50_Q7XZ78 Cluster: Hydromethyl transferase; n=1; Griffiths... 33 5.4
UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barre... 33 7.1
UniRef50_Q7RQX7 Cluster: Serine hydroxymethyltransferase; n=4; P... 33 7.1
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la... 33 7.1
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba
histolytica HM-1:IMSS
Length = 86
Score = 75.4 bits (177), Expect = 1e-12
Identities = 33/34 (97%), Positives = 34/34 (100%)
Frame = +2
Query: 512 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIT 613
HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVI+
Sbjct: 5 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIS 38
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 68.9 bits (161), Expect = 9e-11
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +1
Query: 529 QRRDWENPGVTQLNRLAAHPPFASWRNNE 615
QRRDWENPGVTQLNRLAAHPPFASWRN+E
Sbjct: 73 QRRDWENPGVTQLNRLAAHPPFASWRNSE 101
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 68.9 bits (161), Expect = 9e-11
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +1
Query: 529 QRRDWENPGVTQLNRLAAHPPFASWRNNE 615
QRRDWENPGVTQLNRLAAHPPFASWRN+E
Sbjct: 27 QRRDWENPGVTQLNRLAAHPPFASWRNSE 55
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 68.9 bits (161), Expect = 9e-11
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +1
Query: 529 QRRDWENPGVTQLNRLAAHPPFASWRNNE 615
QRRDWENPGVTQLNRLAAHPPFASWRN+E
Sbjct: 31 QRRDWENPGVTQLNRLAAHPPFASWRNSE 59
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 68.9 bits (161), Expect = 9e-11
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +1
Query: 529 QRRDWENPGVTQLNRLAAHPPFASWRNNE 615
QRRDWENPGVTQLNRLAAHPPFASWRN+E
Sbjct: 13 QRRDWENPGVTQLNRLAAHPPFASWRNSE 41
>UniRef50_P34896 Cluster: Serine hydroxymethyltransferase,
cytosolic; n=86; root|Rep: Serine
hydroxymethyltransferase, cytosolic - Homo sapiens
(Human)
Length = 483
Score = 53.6 bits (123), Expect = 4e-06
Identities = 29/53 (54%), Positives = 35/53 (66%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLGQFSSLTL*RLRQTERVISAHQIAKG 307
LE CS+ACNKNT PGD SAL PSG+RLG +LT L + + AH I +G
Sbjct: 377 LEACSIACNKNTCPGDRSALRPSGLRLGT-PALTSRGLLEKDFQKVAHFIHRG 428
>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
Beta-galactosidase - Yersinia pseudotuberculosis
Length = 1066
Score = 51.2 bits (117), Expect = 2e-05
Identities = 20/28 (71%), Positives = 23/28 (82%)
Frame = +1
Query: 532 RRDWENPGVTQLNRLAAHPPFASWRNNE 615
RRDWENP +TQ +RL AHPPF SWR+ E
Sbjct: 21 RRDWENPQITQYHRLEAHPPFHSWRDVE 48
>UniRef50_A4SBB9 Cluster: Serine hydroxymethyltransferase; n=11;
Viridiplantae|Rep: Serine hydroxymethyltransferase -
Ostreococcus lucimarinus CCE9901
Length = 525
Score = 48.8 bits (111), Expect = 1e-04
Identities = 19/28 (67%), Positives = 24/28 (85%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLG 382
LEL +ACNKNTVPGD+SA+ P G+R+G
Sbjct: 413 LELAHIACNKNTVPGDVSAMVPGGLRIG 440
>UniRef50_Q5C0V4 Cluster: SJCHGC07535 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC07535 protein - Schistosoma
japonicum (Blood fluke)
Length = 218
Score = 45.6 bits (103), Expect = 0.001
Identities = 24/53 (45%), Positives = 32/53 (60%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLGQFSSLTL*RLRQTERVISAHQIAKG 307
LEL +A NKNT PGD+SAL P G+R G ++LT R+ + + A I G
Sbjct: 113 LELVRIAANKNTCPGDLSALRPGGLRFGS-AALTSRNFREKDFIKVAEFIHTG 164
>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
pneumoniae
Length = 1034
Score = 44.0 bits (99), Expect = 0.003
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +1
Query: 532 RRDWENPGVTQLNRLAAHPPFASWRN 609
R DW N +T LNRL AHP FASWR+
Sbjct: 20 REDWHNQTITHLNRLPAHPVFASWRD 45
>UniRef50_A6MJY3 Cluster: Mitochondrial serine
hydroxymethyltransferase-like protein; n=5;
Euteleostomi|Rep: Mitochondrial serine
hydroxymethyltransferase-like protein - Callithrix
jacchus (Common marmoset)
Length = 122
Score = 42.7 bits (96), Expect = 0.007
Identities = 18/28 (64%), Positives = 21/28 (75%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLG 382
LEL S+ NKNT PGD SA+ P G+RLG
Sbjct: 18 LELVSITANKNTCPGDRSAITPGGLRLG 45
>UniRef50_Q5BJF5 Cluster: Serine hydroxymethyltransferase; n=3;
Euarchontoglires|Rep: Serine hydroxymethyltransferase -
Homo sapiens (Human)
Length = 480
Score = 42.7 bits (96), Expect = 0.007
Identities = 18/28 (64%), Positives = 21/28 (75%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLG 382
LEL S+ NKNT PGD SA+ P G+RLG
Sbjct: 376 LELVSITANKNTCPGDRSAITPGGLRLG 403
>UniRef50_P34897 Cluster: Serine hydroxymethyltransferase,
mitochondrial precursor; n=160; Eukaryota|Rep: Serine
hydroxymethyltransferase, mitochondrial precursor - Homo
sapiens (Human)
Length = 504
Score = 42.7 bits (96), Expect = 0.007
Identities = 18/28 (64%), Positives = 21/28 (75%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLG 382
LEL S+ NKNT PGD SA+ P G+RLG
Sbjct: 400 LELVSITANKNTCPGDRSAITPGGLRLG 427
>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 1043
Score = 40.3 bits (90), Expect = 0.036
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +1
Query: 532 RRDWENPGVTQLNRLAAHPPFASWRN 609
R DW+NP +T +NRL +H P WR+
Sbjct: 24 RNDWQNPAITSVNRLPSHTPLHGWRD 49
>UniRef50_O23984 Cluster: Expressed sequence tag; n=7; Poaceae|Rep:
Expressed sequence tag - Hordeum vulgare (Barley)
Length = 111
Score = 40.3 bits (90), Expect = 0.036
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = -1
Query: 462 ELCSVACNKNTVPGDISALNPSGIRLG 382
+LCS+ NKN V GD SAL+P G+R+G
Sbjct: 6 DLCSITLNKNAVFGDSSALSPGGVRIG 32
>UniRef50_UPI0000E49DF3 Cluster: PREDICTED: similar to serine
hydroxymethyltransferase isoform 1; n=4; Coelomata|Rep:
PREDICTED: similar to serine hydroxymethyltransferase
isoform 1 - Strongylocentrotus purpuratus
Length = 496
Score = 39.5 bits (88), Expect = 0.062
Identities = 21/53 (39%), Positives = 30/53 (56%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLGQFSSLTL*RLRQTERVISAHQIAKG 307
LE + NKNT PGD SAL P G+R+G +LT + T+ ++ I +G
Sbjct: 390 LERVGIVLNKNTCPGDKSALKPGGLRIGT-PALTSRNFKVTDFMMVVDYIDRG 441
>UniRef50_Q9U638 Cluster: SHMT; n=5; Aconoidasida|Rep: SHMT -
Plasmodium falciparum
Length = 442
Score = 37.9 bits (84), Expect = 0.19
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = -1
Query: 453 SVACNKNTVPGDISALNPSGIRLG 382
++A NKNT+P D+ ++PSGIR+G
Sbjct: 350 NIALNKNTIPSDVDCVSPSGIRIG 373
>UniRef50_Q8ZYF9 Cluster: Serine hydroxymethyltransferase; n=5;
Thermoproteaceae|Rep: Serine hydroxymethyltransferase -
Pyrobaculum aerophilum
Length = 430
Score = 34.7 bits (76), Expect = 1.8
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDISALNPSGIRLG 382
LE ++ NKN +P D S L PSGIR+G
Sbjct: 334 LEEANIIVNKNALPWDKSVLKPSGIRMG 361
>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
Gammaproteobacteria|Rep: Beta-galactosidase -
Pseudoalteromonas haloplanktis (Alteromonas
haloplanktis)
Length = 1039
Score = 34.3 bits (75), Expect = 2.3
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1
Query: 532 RRDWENPGVTQLNRLAAHPPFASWRNNE 615
RRDWENP Q+N++ AH P ++ E
Sbjct: 10 RRDWENPITVQVNQVKAHSPLNGFKTIE 37
>UniRef50_Q8EWD1 Cluster: Serine hydroxymethyltransferase; n=14;
cellular organisms|Rep: Serine hydroxymethyltransferase
- Mycoplasma penetrans
Length = 412
Score = 33.9 bits (74), Expect = 3.1
Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -1
Query: 465 LELCSVACNKNTVPGDI-SALNPSGIRLGQFSSLT 364
LE + NKN +P DI SA +PSGIR+G + T
Sbjct: 328 LEQAKIVVNKNLIPYDINSAKSPSGIRIGSAAMTT 362
>UniRef50_A7FMW0 Cluster: Opacity-associated protein A family; n=16;
Enterobacteriaceae|Rep: Opacity-associated protein A
family - Yersinia pseudotuberculosis IP 31758
Length = 246
Score = 33.5 bits (73), Expect = 4.1
Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
Frame = +2
Query: 419 SPGTVFLLQATLQSSRGGPVPN--SPYSESYYIHWPSFYNVVTGKTLA 556
+P T LQA LQ + PVP SP SE+ +W S Y V TGKTLA
Sbjct: 129 APSTSVPLQAQLQDNNDVPVPPKVSP-SETTQGNWQS-YQVQTGKTLA 174
>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
uncultured bacterium|Rep: Non-ribosomal peptide
synthetase - uncultured bacterium
Length = 338
Score = 33.5 bits (73), Expect = 4.1
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = -1
Query: 504 YDSL*GELGTGPPLEL 457
YDSL GELGTGPPLE+
Sbjct: 278 YDSLYGELGTGPPLEV 293
>UniRef50_Q7XZ78 Cluster: Hydromethyl transferase; n=1; Griffithsia
japonica|Rep: Hydromethyl transferase - Griffithsia
japonica (Red alga)
Length = 100
Score = 33.1 bits (72), Expect = 5.4
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -1
Query: 429 VPGDISALNPSGIRLGQFSSLT 364
VPGD+SA NP GIR+G + T
Sbjct: 1 VPGDVSAFNPGGIRMGTHAMTT 22
>UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barrel;
n=1; Clostridium cellulolyticum H10|Rep: Glycoside
hydrolase family 2, TIM barrel - Clostridium
cellulolyticum H10
Length = 1033
Score = 32.7 bits (71), Expect = 7.1
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +1
Query: 535 RDWENPGVTQLNRLAAHPPFASWRNNE 615
R+WEN +TQ+NR H P+ ++ + E
Sbjct: 3 REWENQYITQINRYPMHSPYGAYESVE 29
>UniRef50_Q7RQX7 Cluster: Serine hydroxymethyltransferase; n=4;
Plasmodium|Rep: Serine hydroxymethyltransferase -
Plasmodium yoelii yoelii
Length = 446
Score = 32.7 bits (71), Expect = 7.1
Identities = 16/36 (44%), Positives = 24/36 (66%), Gaps = 4/36 (11%)
Frame = -1
Query: 477 TGPPL-ELCS---VACNKNTVPGDISALNPSGIRLG 382
TG L E+C+ ++ NKNT+P D ++P+G RLG
Sbjct: 342 TGSKLQEVCNTINISINKNTIPSDNDCVSPNGARLG 377
>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
lactis|Rep: Beta-galactosidase - Lactococcus lactis
subsp. lactis (Streptococcus lactis)
Length = 998
Score = 32.7 bits (71), Expect = 7.1
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +1
Query: 529 QRRDWENPGVTQLNRLAAHPP 591
+R+DWENP V+ NRL H P
Sbjct: 11 ERKDWENPVVSNWNRLPMHTP 31
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 553,338,875
Number of Sequences: 1657284
Number of extensions: 9977229
Number of successful extensions: 24883
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 24232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24868
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 44392209541
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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