BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0209 (720 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 164 1e-41 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 162 5e-41 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 97 2e-21 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 5e-13 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.5 SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 2.7 SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 27 2.7 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.2 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.2 SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 25 8.2 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 164 bits (399), Expect = 1e-41 Identities = 83/166 (50%), Positives = 102/166 (61%), Gaps = 2/166 (1%) Frame = +1 Query: 223 FQHFLQRDRSCKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 402 F F K+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108 Query: 403 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGVPA--LGSLPY*WSVSPLTTARSLN 576 T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGG G+L T L Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQ 168 Query: 577 WSSPSTPRLRXXXXXXXXXXXXXXXXXXXXXXDCAFMVDNEAIYDI 714 +S P++ DC FMVDNE+ YDI Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLA--DCTFMVDNESCYDI 212 Score = 94.3 bits (224), Expect = 2e-20 Identities = 41/63 (65%), Positives = 45/63 (71%) Frame = +2 Query: 80 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 259 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 260 TYP 268 P Sbjct: 61 YVP 63 Score = 70.5 bits (165), Expect = 2e-13 Identities = 30/44 (68%), Positives = 39/44 (88%) Frame = +3 Query: 510 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVFTGVVEPYNFI 641 TGSGF +LL+ERL+++Y KKSKL+F++YPAPQV T VVEPYN + Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 162 bits (393), Expect = 5e-41 Identities = 78/164 (47%), Positives = 98/164 (59%) Frame = +1 Query: 223 FQHFLQRDRSCKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 402 F F K VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112 Query: 403 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGVPALGSLPY*WSVSPLTTARSLNWS 582 T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGG G + + N Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQ 172 Query: 583 SPSTPRLRXXXXXXXXXXXXXXXXXXXXXXDCAFMVDNEAIYDI 714 P + DC FMVDNEA YDI Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDI 216 Score = 88.2 bits (209), Expect = 1e-18 Identities = 45/68 (66%), Positives = 47/68 (69%), Gaps = 5/68 (7%) Frame = +2 Query: 80 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 244 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 245 TGAASTYP 268 TG P Sbjct: 60 TGQGKFVP 67 Score = 69.3 bits (162), Expect = 5e-13 Identities = 29/44 (65%), Positives = 38/44 (86%) Frame = +3 Query: 510 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVFTGVVEPYNFI 641 TGSG +LL+ERL+++YGKKS L+F++YPAPQV T VVEPYN + Sbjct: 149 TGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 97.5 bits (232), Expect = 2e-21 Identities = 43/84 (51%), Positives = 58/84 (69%) Frame = +1 Query: 256 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVL 435 K+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VL Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117 Query: 436 DRIRKLADQCTGLQGFLIFHSFGG 507 D +R+ A+ C LQGF + HS GG Sbjct: 118 DVVRREAEACDALQGFQLTHSLGG 141 Score = 39.5 bits (88), Expect = 5e-04 Identities = 17/42 (40%), Positives = 26/42 (61%) Frame = +3 Query: 510 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVFTGVVEPYN 635 TGSG +LL+ ++ +Y + F++ PAP+ VVEPYN Sbjct: 143 TGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYN 184 Score = 36.3 bits (80), Expect = 0.004 Identities = 19/63 (30%), Positives = 26/63 (41%) Frame = +2 Query: 80 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 259 MRE + + GQ G Q+G A W EHG+ G T + N +F+E Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 260 TYP 268 P Sbjct: 59 YVP 61 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 69.3 bits (162), Expect = 5e-13 Identities = 35/94 (37%), Positives = 57/94 (60%), Gaps = 2/94 (2%) Frame = +1 Query: 232 FLQRDRSCKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 405 F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109 Query: 406 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 507 + I + ++D I + AD L+GF + HS G Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAG 143 Score = 48.8 bits (111), Expect = 8e-07 Identities = 24/55 (43%), Positives = 33/55 (60%) Frame = +2 Query: 83 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 247 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55 Score = 37.1 bits (82), Expect = 0.003 Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%) Frame = +3 Query: 510 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VFTGVVEPYN 635 TGSG S L+ERL+ Y KK ++++P Q V VV+PYN Sbjct: 145 TGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 27.9 bits (59), Expect = 1.5 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = -1 Query: 315 ADLINNSRFKIDEDS--TGYVLAAPVSLKKVLKESSP 211 AD+IN SR+ ID S T + P+SL KE P Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491 >SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 830 Score = 27.1 bits (57), Expect = 2.7 Identities = 14/46 (30%), Positives = 25/46 (54%) Frame = -1 Query: 567 SCRSQRRDAPSIRK*TQSRYSTEGVEDQESLQTGTLVSELADSVQN 430 S + SIR ++SR S+ G + Q S+Q+GT ++D ++ Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143 >SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 27.1 bits (57), Expect = 2.7 Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%) Frame = -1 Query: 348 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIW 181 F + + S +++N+ + KID GY S K + E + P + G+W Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.2 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +1 Query: 178 WPDAHRQDHRGWRRFFQHFLQR 243 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 8.2 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 69 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 191 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 >SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1067 Score = 25.4 bits (53), Expect = 8.2 Identities = 12/37 (32%), Positives = 22/37 (59%) Frame = -1 Query: 282 DEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIWPSG 172 ++D T Y L P+ + L +++PP +V+ +G P G Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,044,122 Number of Sequences: 5004 Number of extensions: 64655 Number of successful extensions: 229 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 227 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 337208592 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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