Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0189
(591 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 143 2e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 142 3e-35
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 84 2e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 7e-10
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 29 0.67
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 4.7
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 25 6.2
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 25 6.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 6.2
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 8.3
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 8.3
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 143 bits (346), Expect = 2e-35
Identities = 65/110 (59%), Positives = 81/110 (73%)
Frame = +3
Query: 255 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIR 434
R+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR
Sbjct: 64 RSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIR 123
Query: 435 KLADQCTGLQGFLIFHXXXXXXXXXXLPY*WSVSPVDYGKKSKLEFRHLP 584
++AD C+GLQGFL+FH ++Y KKSKL+F P
Sbjct: 124 RIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP 173
Score = 102 bits (244), Expect = 4e-23
Identities = 44/63 (69%), Positives = 49/63 (77%)
Frame = +1
Query: 67 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 246
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 247 HVP 255
+VP
Sbjct: 61 YVP 63
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 142 bits (345), Expect = 3e-35
Identities = 62/110 (56%), Positives = 82/110 (74%)
Frame = +3
Query: 255 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIR 434
R+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR
Sbjct: 68 RSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIR 127
Query: 435 KLADQCTGLQGFLIFHXXXXXXXXXXLPY*WSVSPVDYGKKSKLEFRHLP 584
++AD C+GLQGFL+FH ++YGKKS L+F P
Sbjct: 128 RMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP 177
Score = 95.1 bits (226), Expect = 7e-21
Identities = 48/68 (70%), Positives = 50/68 (73%), Gaps = 5/68 (7%)
Frame = +1
Query: 67 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 231
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 232 TGAGKHVP 255
TG GK VP
Sbjct: 60 TGQGKFVP 67
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 83.8 bits (198), Expect = 2e-17
Identities = 37/76 (48%), Positives = 51/76 (67%)
Frame = +3
Query: 255 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIR 434
RAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R
Sbjct: 62 RAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVR 121
Query: 435 KLADQCTGLQGFLIFH 482
+ A+ C LQGF + H
Sbjct: 122 REAEACDALQGFQLTH 137
Score = 44.4 bits (100), Expect = 1e-05
Identities = 22/63 (34%), Positives = 30/63 (47%)
Frame = +1
Query: 67 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 246
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 247 HVP 255
+VP
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.4 bits (135), Expect = 7e-10
Identities = 29/78 (37%), Positives = 47/78 (60%), Gaps = 2/78 (2%)
Frame = +3
Query: 255 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDR 428
RA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 429 IRKLADQCTGLQGFLIFH 482
I + AD L+GF + H
Sbjct: 122 IDREADGSDSLEGFSLLH 139
Score = 52.4 bits (120), Expect = 5e-08
Identities = 25/62 (40%), Positives = 37/62 (59%)
Frame = +1
Query: 70 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 249
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 250 VP 255
+P
Sbjct: 61 IP 62
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 28.7 bits (61), Expect = 0.67
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -2
Query: 302 ADLINNSRFKIDEDSTGTCLPAPVSLKKVLKESSPPPM 189
AD+IN SR+ ID S T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 4.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 146 STASSLMARCPQTRPSGVETILSTLSSARPELAST 250
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 4.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 165 WPDAHRQDHRGWRRFFQHFLQR 230
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 25.4 bits (53), Expect = 6.2
Identities = 12/36 (33%), Positives = 23/36 (63%)
Frame = -2
Query: 524 SIRK*TRAGTSTEGVEDQESLQTGTLVSELADSVQN 417
SIR +R+ +S+ G + Q S+Q+GT ++D ++
Sbjct: 108 SIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 25.4 bits (53), Expect = 6.2
Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = +1
Query: 490 VEVPALVHFLID--GASLPLTTARSLNWSFA-IYPAP 591
++ ++ +ID G+S+PL ++L W F IY AP
Sbjct: 335 IDYKRILEIIIDHLGSSVPLIQEKALKWLFEFIYIAP 371
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 6.2
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 56 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 178
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 25.0 bits (52), Expect = 8.3
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -1
Query: 120 ITDLDSGLANVYRDALTHFDLSF 52
+T +D GLA YRD TH + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.0 bits (52), Expect = 8.3
Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 1/59 (1%)
Frame = +1
Query: 418 FWTESASSLTSVPVCKDS*SSTPSVEVPALVHFLIDGASLPLTTARSLNW-SFAIYPAP 591
F +S+S +PV S + S ++P L+ L+D A LP L W + + P P
Sbjct: 642 FHPKSSSWTYDLPVINRSFEALSSYDIPPLLASLLD-ALLPARCIEFLLWAALLVEPFP 699
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,507,936
Number of Sequences: 5004
Number of extensions: 52173
Number of successful extensions: 172
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 256184654
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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