BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0174 (542 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 64 2e-11 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 62 5e-11 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 59 5e-10 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 37 0.002 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.003 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 36 0.004 SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 33 0.027 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.34 SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 28 1.0 SPCC126.08c |||lectin |Schizosaccharomyces pombe|chr 3|||Manual 26 3.1 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 26 3.1 SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 26 4.1 SPBC1711.10c |npl4||Cdc48-Ufd1-Npl4 complex component Npl4 |Schi... 25 5.5 SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyce... 25 5.5 SPBP8B7.12c |fta3|sma3|Sim4 and Mal2 associated |Schizosaccharom... 25 7.2 SPAC11E3.13c |||1,3-beta-glucanosyltransferase |Schizosaccharomy... 25 7.2 SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1||... 25 7.2 SPCC1682.03c |mug174||meiotically upregulated gene Mug174|Schizo... 25 7.2 SPBC19F5.02c |||U3 snoRNP protein Utp4 |Schizosaccharomyces pomb... 25 9.5 SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual 25 9.5 SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 25 9.5 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 63.7 bits (148), Expect = 2e-11 Identities = 30/75 (40%), Positives = 41/75 (54%), Gaps = 4/75 (5%) Frame = +3 Query: 252 TWCGHCKSLVPEYKKAARALK--GIVKVGALDADEHRSVSQKYGVTGFPTIKIF--TGSK 419 TWCGHCKSL P Y++ + V +G +DAD H V+ KY +TGFPT+ F GS+ Sbjct: 49 TWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSE 108 Query: 420 HTPYQGQRTAEGFVE 464 Y R + + Sbjct: 109 PVQYSNARDVDSLTQ 123 Score = 51.2 bits (117), Expect = 1e-07 Identities = 26/75 (34%), Positives = 39/75 (52%), Gaps = 5/75 (6%) Frame = +3 Query: 255 WCGHCKSLVPEYKKAARALKG--IVKVGALDADEHRSVSQKYGVTGFPTIKIF-TGSKHT 425 WCG+CK L P Y+ + K V++ ++AD + + + V FPTIK F K Sbjct: 169 WCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDK 228 Query: 426 P--YQGQRTAEGFVE 464 P Y+G R+ E +E Sbjct: 229 PELYEGDRSLESLIE 243 Score = 31.5 bits (68), Expect = 0.083 Identities = 15/44 (34%), Positives = 25/44 (56%) Frame = +1 Query: 169 SDVIELTPSNFDKLLTNSDEIWIIEFFAPGVDIVKALFPNIKKL 300 S+V+EL NFDK++ + + ++EF+A K L P + L Sbjct: 140 SNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETL 183 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 62.1 bits (144), Expect = 5e-11 Identities = 33/72 (45%), Positives = 45/72 (62%), Gaps = 8/72 (11%) Frame = +3 Query: 255 WCGHCKSLVPEYKKAARALKGIVKVGAL--DADEHRSVSQKYGVTGFPTIK-IFTGSK-- 419 WCG+CK LVP Y+K A L ++ V A+ DAD++R+V +Y V GFPTIK ++ SK Sbjct: 59 WCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGS 118 Query: 420 ---HTPYQGQRT 446 T Y G R+ Sbjct: 119 SLSSTDYNGDRS 130 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 58.8 bits (136), Expect = 5e-10 Identities = 26/72 (36%), Positives = 41/72 (56%), Gaps = 2/72 (2%) Frame = +3 Query: 255 WCGHCKSLVPEYKKAARAL-KGIVKVGALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTP 428 WCGHCK+L PEY+ AA L K + + +D E + +Y + G+PT+ +F G + + Sbjct: 50 WCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQ 109 Query: 429 YQGQRTAEGFVE 464 Y G R + V+ Sbjct: 110 YSGPRKHDALVK 121 Score = 35.5 bits (78), Expect = 0.005 Identities = 16/49 (32%), Positives = 27/49 (55%) Frame = +1 Query: 160 DSSSDVIELTPSNFDKLLTNSDEIWIIEFFAPGVDIVKALFPNIKKLPE 306 +S D++ L NFD ++ + + ++EF+AP K L P +KL E Sbjct: 352 ESQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAE 400 Score = 26.6 bits (56), Expect = 2.4 Identities = 12/41 (29%), Positives = 27/41 (65%) Frame = +1 Query: 163 SSSDVIELTPSNFDKLLTNSDEIWIIEFFAPGVDIVKALFP 285 +S++V ++ ++L+T +D++ +++F+AP KAL P Sbjct: 20 ASAEVPKVNKEGLNELIT-ADKVLMVKFYAPWCGHCKALAP 59 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 37.1 bits (82), Expect = 0.002 Identities = 18/51 (35%), Positives = 29/51 (56%) Frame = +3 Query: 255 WCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIF 407 WCG CK L P +K + + + A++AD+ ++QK GV PT+ +F Sbjct: 46 WCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPTMVLF 95 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 36.3 bits (80), Expect = 0.003 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 1/56 (1%) Frame = +3 Query: 255 WCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTGSK 419 WCG CK++ P + + A V A ++ DE R ++ GV PT F K Sbjct: 30 WCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGK 85 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 35.9 bits (79), Expect = 0.004 Identities = 19/77 (24%), Positives = 39/77 (50%), Gaps = 1/77 (1%) Frame = +3 Query: 252 TWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTP 428 TWCG CK++ P++++ + + +D D+ ++ + GV P+ ++ G K Sbjct: 28 TWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEE 86 Query: 429 YQGQRTAEGFVEAALNA 479 G A+ +EA++ A Sbjct: 87 IVGANPAK--LEASIKA 101 >SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 764 Score = 33.1 bits (72), Expect = 0.027 Identities = 17/37 (45%), Positives = 20/37 (54%) Frame = +1 Query: 139 TGSLALYDSSSDVIELTPSNFDKLLTNSDEIWIIEFF 249 TGS + SDV+ L+ S LL SD WII FF Sbjct: 428 TGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFF 464 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 29.5 bits (63), Expect = 0.34 Identities = 13/60 (21%), Positives = 24/60 (40%) Frame = +3 Query: 258 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 437 C C + R +K+ ++ DE + + + + FPT ++F G Y G Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268 >SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 27.9 bits (59), Expect = 1.0 Identities = 14/25 (56%), Positives = 17/25 (68%) Frame = +3 Query: 465 AALNAAKEKAYENLGKKSSGYSSDK 539 AALNAAKE E L +K+ SSD+ Sbjct: 314 AALNAAKENEAERLKRKNQVTSSDR 338 >SPCC126.08c |||lectin |Schizosaccharomyces pombe|chr 3|||Manual Length = 312 Score = 26.2 bits (55), Expect = 3.1 Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 1/45 (2%) Frame = +3 Query: 381 TGFPTIKIFTGSKHTPYQGQRTAEGFVEAALNAAKEKAYE-NLGK 512 T FP + + G HTPY + + A+ +A + + NLGK Sbjct: 142 TLFPRVIVMKGDGHTPYDYENDGKANEIASCSALNVRGNDYNLGK 186 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 26.2 bits (55), Expect = 3.1 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +1 Query: 106 HGYSIGILLCATGSLALYDSSSDVIELTPSNFDKLLTNSD 225 HG ++G L T + +SSD E + SN D+ L +S+ Sbjct: 9 HGQALGSTLNHTTEVTRISNSSDHFEDSSSNVDESLDSSN 48 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.8 bits (54), Expect = 4.1 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = +3 Query: 102 VTRIFNRYLALCNGVLGPLRFVFRRY 179 V I N L +C GV PLR +F R+ Sbjct: 75 VREIMNDLLDMCRGVQHPLRGLFLRH 100 >SPBC1711.10c |npl4||Cdc48-Ufd1-Npl4 complex component Npl4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 545 Score = 25.4 bits (53), Expect = 5.5 Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 1/69 (1%) Frame = -1 Query: 395 CGEASDSIFL*YTSMFISI*-GTYFYNAL*SSGSFFIFGNKAFTMSTPGAKNSMIQISSE 219 C SDS FL ++ S T F N S S + FG+K T G N I++ S Sbjct: 343 CKRHSDSYFLSSLEVYNSANFQTKFKNPCKWSRSGY-FGSKFVTSVISGNLNGEIEVMSY 401 Query: 218 FVSNLSKLL 192 VSN+ L Sbjct: 402 QVSNIGTAL 410 >SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 138 Score = 25.4 bits (53), Expect = 5.5 Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 1/51 (1%) Frame = +3 Query: 264 HCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGV-TGFPTIKIFTG 413 HC KAAR L I++ ++ E S+SQK + PT+ I G Sbjct: 69 HCTYSQVRGPKAARVLSEILRNRITESKEKLSLSQKEKLFQNLPTVYILHG 119 >SPBP8B7.12c |fta3|sma3|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 2|||Manual Length = 220 Score = 25.0 bits (52), Expect = 7.2 Identities = 13/58 (22%), Positives = 28/58 (48%), Gaps = 1/58 (1%) Frame = +3 Query: 372 YGVTGFPTIKIFTGSKHTPYQGQRTAEGFVEAALNAAKEKAYENLGKKSS-GYSSDKV 542 + + F T G + + + GF+ + LN + + ENL +SS ++S+++ Sbjct: 72 FSILNFMTTNSLVGQLYQRLYDESNSLGFISSPLNETEHLSEENLKIESSITFTSEEI 129 >SPAC11E3.13c |||1,3-beta-glucanosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 25.0 bits (52), Expect = 7.2 Identities = 18/54 (33%), Positives = 25/54 (46%) Frame = +1 Query: 103 LHGYSIGILLCATGSLALYDSSSDVIELTPSNFDKLLTNSDEIWIIEFFAPGVD 264 LH + +LL LAL DS+S I++ + F TN F+ GVD Sbjct: 4 LHFLTTSLLLLGGSRLALADSASSAIKIKGNAFFNSDTNE------RFYVRGVD 51 >SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 654 Score = 25.0 bits (52), Expect = 7.2 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +1 Query: 331 VP*MLMNIEVYHRNMESLASPQLRYSQ 411 VP +L+N+E +H+ ++ A+P + +Q Sbjct: 134 VPPLLLNVESHHKVLDMCAAPGSKTAQ 160 >SPCC1682.03c |mug174||meiotically upregulated gene Mug174|Schizosaccharomyces pombe|chr 3|||Manual Length = 626 Score = 25.0 bits (52), Expect = 7.2 Identities = 15/35 (42%), Positives = 18/35 (51%) Frame = -1 Query: 263 STPGAKNSMIQISSEFVSNLSKLLGVSSITSEDES 159 STP +S SS VS+ S L S +S DES Sbjct: 260 STPSESDSSSSESSSSVSDSSDLSSTSDSSSGDES 294 >SPBC19F5.02c |||U3 snoRNP protein Utp4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 710 Score = 24.6 bits (51), Expect = 9.5 Identities = 26/102 (25%), Positives = 39/102 (38%), Gaps = 10/102 (9%) Frame = +3 Query: 90 KFHNVTRIFNRYLA--LCNGVLGPLRFVFR--------RYRADTE*FRQITYKFRRNLDH 239 KF +++ F +LA LC GV VF +Y + ++ FRR H Sbjct: 234 KFFTLSQSFKLHLADALCLGVSANGDMVFSSGIDRKTIQYSREGGKREWVSNSFRRFHSH 293 Query: 240 *VLCTWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVS 365 V C CKSL + ++ V + HR +S Sbjct: 294 DVRCMAVFECKSLDVLISGGMDMMLAVIPVRQFNRKNHRMIS 335 >SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 684 Score = 24.6 bits (51), Expect = 9.5 Identities = 15/36 (41%), Positives = 19/36 (52%) Frame = -1 Query: 233 QISSEFVSNLSKLLGVSSITSEDES*RAKDPVAQSK 126 Q SS +N SKL +SS SEDE +D A + Sbjct: 638 QSSSNSRANPSKLFNISSDDSEDEVPIIEDTTASDE 673 >SPBC800.10c |||EPS15 repeat family actin cortical patch component |Schizosaccharomyces pombe|chr 2|||Manual Length = 1116 Score = 24.6 bits (51), Expect = 9.5 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = -1 Query: 263 STPGAKNSMIQISSEFVSNLSKLLGVSSITSEDES*RAKDPVAQSKIPIEY 111 S S Q S +S+LSKL G + T+E + +P+ + P + Sbjct: 844 SVVDVSTSASQRGSPVLSDLSKLTGSARNTAEPVENTSAEPIENTSAPTPF 894 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,269,650 Number of Sequences: 5004 Number of extensions: 46867 Number of successful extensions: 154 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 223909422 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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