BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0125 (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 175 7e-45 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 103 2e-23 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 101 1e-22 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 94 1e-20 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 69 5e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 64 1e-11 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 53 3e-08 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 44 2e-05 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.090 SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 30 0.28 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.36 SPCC24B10.03 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 27 2.6 SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 2.6 SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po... 27 2.6 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.4 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 27 3.4 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 4.5 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.5 SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 26 4.5 SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 26 5.9 SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|ch... 26 5.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 175 bits (425), Expect = 7e-45 Identities = 78/86 (90%), Positives = 82/86 (95%) Frame = +1 Query: 250 QKGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 429 ++GILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT Sbjct: 61 KRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMT 120 Query: 430 QIMFETFNTPAMYVAIQAVLSLYASG 507 QI+FETFN PA YVAIQAVLSLYASG Sbjct: 121 QIIFETFNAPAFYVAIQAVLSLYASG 146 Score = 121 bits (291), Expect = 1e-28 Identities = 53/58 (91%), Positives = 57/58 (98%) Frame = +2 Query: 74 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 247 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQ Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQ 59 Score = 62.1 bits (144), Expect = 7e-11 Identities = 27/29 (93%), Positives = 28/29 (96%) Frame = +3 Query: 510 TTGIVLDSGDGVFHTVPIYEGYALPHAIL 596 TTGIVLDSGDGV HTVPIYEGYALPHAI+ Sbjct: 148 TTGIVLDSGDGVTHTVPIYEGYALPHAIM 176 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 103 bits (248), Expect = 2e-23 Identities = 43/86 (50%), Positives = 65/86 (75%) Frame = +1 Query: 253 KGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 432 + +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 433 IMFETFNTPAMYVAIQAVLSLYASGL 510 MFE + +YVAIQAVLSLYA GL Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQGL 147 Score = 54.0 bits (124), Expect = 2e-08 Identities = 29/58 (50%), Positives = 37/58 (63%), Gaps = 2/58 (3%) Frame = +2 Query: 80 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQ 247 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA+ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAE 59 Score = 40.3 bits (90), Expect = 3e-04 Identities = 15/28 (53%), Positives = 21/28 (75%) Frame = +3 Query: 510 TTGIVLDSGDGVFHTVPIYEGYALPHAI 593 ++G+V+DSGDGV H VP+YE L H + Sbjct: 148 SSGVVVDSGDGVTHIVPVYESVVLNHLV 175 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 101 bits (241), Expect = 1e-22 Identities = 43/80 (53%), Positives = 55/80 (68%) Frame = +1 Query: 265 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 444 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 445 TFNTPAMYVAIQAVLSLYAS 504 +FN +Y+A+QAVL+L AS Sbjct: 149 SFNCAGLYIAVQAVLALAAS 168 Score = 42.7 bits (96), Expect = 5e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +2 Query: 92 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 241 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/30 (56%), Positives = 22/30 (73%) Frame = +3 Query: 504 RSTTGIVLDSGDGVFHTVPIYEGYALPHAI 593 RS TG V+DSGDGV H +P+ EGY + +I Sbjct: 177 RSLTGTVVDSGDGVTHIIPVAEGYVIGSSI 206 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 94.3 bits (224), Expect = 1e-20 Identities = 41/85 (48%), Positives = 60/85 (70%), Gaps = 1/85 (1%) Frame = +1 Query: 253 KGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMT 429 +G+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIA 125 Query: 430 QIMFETFNTPAMYVAIQAVLSLYAS 504 + +ET N PA+ ++Q VL+LYAS Sbjct: 126 EYFYETLNVPALSFSLQPVLALYAS 150 Score = 69.3 bits (162), Expect = 5e-13 Identities = 30/52 (57%), Positives = 38/52 (73%) Frame = +2 Query: 92 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 247 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQ 63 Score = 47.2 bits (107), Expect = 2e-06 Identities = 22/39 (56%), Positives = 29/39 (74%) Frame = +3 Query: 477 PSRALAVRVRSTTGIVLDSGDGVFHTVPIYEGYALPHAI 593 P AL R TTGIVL+ GDG+ H+VPIY+G+++P AI Sbjct: 143 PVLALYASAR-TTGIVLECGDGLTHSVPIYDGFSIPSAI 180 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 69.3 bits (162), Expect = 5e-13 Identities = 32/82 (39%), Positives = 46/82 (56%) Frame = +1 Query: 262 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 441 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 442 ETFNTPAMYVAIQAVLSLYASG 507 E+ PA Y+A Q + +ASG Sbjct: 123 ESLRCPATYLAKQETCAAFASG 144 Score = 40.3 bits (90), Expect = 3e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 77 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 178 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 64.5 bits (150), Expect = 1e-11 Identities = 29/82 (35%), Positives = 47/82 (57%) Frame = +1 Query: 277 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 456 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 457 PAMYVAIQAVLSLYASGLPPVS 522 P++ I + S Y + P S Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSS 166 Score = 35.9 bits (79), Expect = 0.006 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 92 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 187 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +3 Query: 507 STTGIVLDSGDGVFHTVPIYEG 572 S++GIVL+ G+ H +P+ G Sbjct: 164 SSSGIVLNLGNAASHVIPVLNG 185 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 53.2 bits (122), Expect = 3e-08 Identities = 22/73 (30%), Positives = 42/73 (57%) Frame = +1 Query: 286 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 465 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 466 YVAIQAVLSLYAS 504 Y+ QAV +A+ Sbjct: 134 YLTKQAVCVAFAN 146 Score = 38.7 bits (86), Expect = 8e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +2 Query: 77 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 247 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 44.0 bits (99), Expect = 2e-05 Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 4/82 (4%) Frame = +1 Query: 277 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 444 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 445 TFNTPAMYVAIQAVLSLYASGL 510 P +A + ++ LYA G+ Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGI 193 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.090 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 337 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 480 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 30.3 bits (65), Expect = 0.28 Identities = 18/56 (32%), Positives = 23/56 (41%) Frame = +1 Query: 514 PVSCWTPATVSSTPCPSTRDTHSPTPSLRLDFGPVRDLTRLPSWKNPSPEPRGTWF 681 P S TP ++T P+T DTH P GP+ D +PS P F Sbjct: 280 PQSGSTPQMQNTTSQPTTHDTHPPKQQ-----GPISDFRSIPSSPKTEGAPSNAQF 330 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.36 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -2 Query: 234 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 73 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPCC24B10.03 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 128 Score = 27.1 bits (57), Expect = 2.6 Identities = 8/18 (44%), Positives = 10/18 (55%) Frame = +1 Query: 634 LPSWKNPSPEPRGTWFHL 687 + WKN P +G W HL Sbjct: 24 MKDWKNSQPNEQGRWLHL 41 >SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 601 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/46 (28%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = -3 Query: 554 GVEDTVAGVQHDTGGRPDA-YSESTAWMATYMAGVLNVSNMIWVIF 420 GV+DT+ + + G+P Y EST + A + N++ + W ++ Sbjct: 402 GVQDTLTAIWNTNSGKPSGWYLESTMYKALDVT-YYNLNTLSWNLY 446 >SPAC824.09c |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 27.1 bits (57), Expect = 2.6 Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 3/40 (7%) Frame = +1 Query: 481 AVLSLYASGLPPV---SCWTPATVSSTPCPSTRDTHSPTP 591 ++LSLYAS P V S T AT + P P + T S P Sbjct: 218 SILSLYASPRPQVSSSSITTNATYQNLPSPVSTSTTSSQP 257 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 3.4 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -2 Query: 237 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 121 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/29 (41%), Positives = 15/29 (51%) Frame = -3 Query: 245 VPHLLHKSPSVPYRPSRPDGGAFPRSRET 159 + L+ K+PS PY SRP A S T Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPT 334 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +2 Query: 137 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 241 G+ PRA F ++ P H G+++ M KD G+E Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 379 LLTEAPLNPKANREKMTQIMFETFN 453 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 26.2 bits (55), Expect = 4.5 Identities = 22/88 (25%), Positives = 37/88 (42%) Frame = +1 Query: 430 QIMFETFNTPAMYVAIQAVLSLYASGLPPVSCWTPATVSSTPCPSTRDTHSPTPSLRLDF 609 QI++ NT ++ A A LY P++ A + P PS+ +T+ P+ S + Sbjct: 234 QILYNN-NTDSI-AAAAATHDLYPQPDVPLTFAMLADGNYVPLPSSTNTYGPSNSYGYEI 291 Query: 610 GPVRDLTRLPSWKNPSPEPRGTWFHLPT 693 + S P P +F LP+ Sbjct: 292 NINESTNHVDSSYLPHPIQLSNYFTLPS 319 >SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 243 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/46 (34%), Positives = 20/46 (43%) Frame = -3 Query: 263 RIPFCSVPHLLHKSPSVPYRPSRPDGGAFPRSRETLREEHHLLRSR 126 R PF S+ H HK YRPSR + + R + RSR Sbjct: 176 RFPFESL-HKNHKHYENSYRPSRSQNNSHYNDKSFHRSRYSRARSR 220 >SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|chr 2|||Manual Length = 397 Score = 25.8 bits (54), Expect = 5.9 Identities = 22/76 (28%), Positives = 31/76 (40%) Frame = +1 Query: 415 REKMTQIMFETFNTPAMYVAIQAVLSLYASGLPPVSCWTPATVSSTPCPSTRDTHSPTPS 594 RE++ Q+ + P+ Y+ LS Y S P + S PST HSP P Sbjct: 134 RERLNQVELQLSERPSSYLGYHNNLSPYRS---------PNSYPSL-LPSTHSPHSPAPL 183 Query: 595 LRLDFGPVRDLTRLPS 642 + +R T PS Sbjct: 184 STMQTALMRLRTYHPS 199 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,178,215 Number of Sequences: 5004 Number of extensions: 73297 Number of successful extensions: 246 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 208 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 243 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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