Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0123
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 182 3e-47
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 178 7e-46
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 105 8e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 82 9e-17
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.0
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 25 7.9
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 182 bits (444), Expect = 3e-47
Identities = 84/143 (58%), Positives = 107/143 (74%)
Frame = +2
Query: 215 FQHFLQRDRSWQALPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 394
F F + +PR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 395 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGLGSLPY*WSVSPLTTARKSKLE 574
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGGTG G + +KSKL+
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQ 168
Query: 575 FAIYPRAFRFPLAVVEPYNFILT 643
F++YP A + +VVEPYN +LT
Sbjct: 169 FSVYP-APQVSTSVVEPYNSVLT 190
Score = 100 bits (239), Expect = 2e-22
Identities = 43/63 (68%), Positives = 48/63 (76%)
Frame = +3
Query: 72 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 251
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 252 HYP 260
+ P
Sbjct: 61 YVP 63
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 178 bits (433), Expect = 7e-46
Identities = 80/143 (55%), Positives = 107/143 (74%)
Frame = +2
Query: 215 FQHFLQRDRSWQALPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 394
F F + +PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 395 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGLGSLPY*WSVSPLTTARKSKLE 574
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGGGTG G + +KS L+
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQ 172
Query: 575 FAIYPRAFRFPLAVVEPYNFILT 643
F++YP A + +VVEPYN +LT
Sbjct: 173 FSVYP-APQVSTSVVEPYNSVLT 194
Score = 93.1 bits (221), Expect = 3e-20
Identities = 47/68 (69%), Positives = 49/68 (72%), Gaps = 5/68 (7%)
Frame = +3
Query: 72 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 236
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 237 TGAGKHYP 260
TG GK P
Sbjct: 60 TGQGKFVP 67
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 105 bits (251), Expect = 8e-24
Identities = 56/130 (43%), Positives = 76/130 (58%)
Frame = +2
Query: 254 LPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDR 433
+PRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 60 VPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDV 119
Query: 434 IRKLADQCTGLQGFLIFHSFGGGTGLGSLPY*WSVSPLTTARKSKLEFAIYPRAFRFPLA 613
+R+ A+ C LQGF + HS GGGTG G S + F++ P A +
Sbjct: 120 VRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAP-APKSSDT 178
Query: 614 VVEPYNFILT 643
VVEPYN L+
Sbjct: 179 VVEPYNATLS 188
Score = 42.3 bits (95), Expect = 6e-05
Identities = 21/63 (33%), Positives = 29/63 (46%)
Frame = +3
Query: 72 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 251
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 252 HYP 260
+ P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 81.8 bits (193), Expect = 9e-17
Identities = 47/141 (33%), Positives = 77/141 (54%), Gaps = 2/141 (1%)
Frame = +2
Query: 224 FLQRDRSWQALPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 397
F Q D + + +PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 398 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGLGSLPY*WSVSPLTTARKSKLEF 577
+ I + ++D I + AD L+GF + HS GGTG G + +K +
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTY 169
Query: 578 AIYPRAFRFPLAVVEPYNFIL 640
+++P + VV+PYN +L
Sbjct: 170 SVFPNSQSVSDVVVQPYNSLL 190
Score = 50.8 bits (116), Expect = 2e-07
Identities = 25/62 (40%), Positives = 36/62 (58%)
Frame = +3
Query: 75 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 254
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 255 YP 260
P
Sbjct: 61 IP 62
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +1
Query: 151 STASSLMARCPQTRPSGVETILSTLSSARPELASTT 258
ST SSL + ++PS T ST SSA P S++
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSS 208
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.0
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 170 WPDAHRQDHRGWRRFFQHFLQR 235
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 25.4 bits (53), Expect = 7.9
Identities = 16/37 (43%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = -1
Query: 307 ADLINNSRFKIDEDS--TG*CLPAPVSLKKVLKESSP 203
AD+IN SR+ ID S T P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 7.9
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 61 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 183
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,090,866
Number of Sequences: 5004
Number of extensions: 67174
Number of successful extensions: 212
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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