BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0122 (621 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 162 4e-41 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 100 3e-22 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 97 2e-21 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 89 6e-19 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 3e-12 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 66 5e-12 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 53 3e-08 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 43 4e-05 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 40 3e-04 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.058 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.31 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 29 0.72 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.9 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.8 SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 3.8 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.1 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 5.1 SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 26 5.1 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 25 8.8 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 162 bits (393), Expect = 4e-41 Identities = 72/78 (92%), Positives = 74/78 (94%) Frame = +1 Query: 256 KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 435 KYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETF Sbjct: 68 KYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETF 127 Query: 436 NTPAMYVAIQAVLSLYAS 489 N PA YVAIQAVLSLYAS Sbjct: 128 NAPAFYVAIQAVLSLYAS 145 Score = 135 bits (326), Expect = 6e-33 Identities = 61/69 (88%), Positives = 65/69 (94%) Frame = +2 Query: 59 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 238 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 239 RGILTPNTP 265 RGILT P Sbjct: 62 RGILTLKYP 70 Score = 45.6 bits (103), Expect = 6e-06 Identities = 23/37 (62%), Positives = 24/37 (64%) Frame = +2 Query: 509 LDSGDGVSHTVPIYEGYRTPPRHSASGT*PGGDLTDY 619 LDSGDGV+HTVPIYEGY P G DLTDY Sbjct: 153 LDSGDGVTHTVPIYEGYALPHAIMRLDL-AGRDLTDY 188 Score = 31.1 bits (67), Expect = 0.13 Identities = 17/36 (47%), Positives = 20/36 (55%) Frame = +3 Query: 489 GRTTGIVWTPATVSPTPCPSTKDTALPHAILRLGLS 596 GRTTGIV P + ALPHAI+RL L+ Sbjct: 146 GRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 99.5 bits (237), Expect = 3e-22 Identities = 42/77 (54%), Positives = 53/77 (68%) Frame = +1 Query: 259 YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN 438 YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN Sbjct: 92 YPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFN 151 Query: 439 TPAMYVAIQAVLSLYAS 489 +Y+A+QAVL+L AS Sbjct: 152 CAGLYIAVQAVLALAAS 168 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +2 Query: 77 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 226 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 Score = 35.1 bits (77), Expect = 0.008 Identities = 15/29 (51%), Positives = 20/29 (68%) Frame = +2 Query: 473 SRCTRRSYHRYRLDSGDGVSHTVPIYEGY 559 S+ T RS +DSGDGV+H +P+ EGY Sbjct: 172 SKVTDRSLTGTVVDSGDGVTHIIPVAEGY 200 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 96.7 bits (230), Expect = 2e-21 Identities = 40/77 (51%), Positives = 59/77 (76%) Frame = +1 Query: 256 KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 435 KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE + Sbjct: 68 KYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERY 127 Query: 436 NTPAMYVAIQAVLSLYA 486 +YVAIQAVLSLYA Sbjct: 128 GFGGVYVAIQAVLSLYA 144 Score = 58.8 bits (136), Expect = 6e-10 Identities = 32/69 (46%), Positives = 42/69 (60%), Gaps = 2/69 (2%) Frame = +2 Query: 65 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 238 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61 Query: 239 RGILTPNTP 265 R +L P Sbjct: 62 RSLLQVKYP 70 Score = 31.1 bits (67), Expect = 0.13 Identities = 11/15 (73%), Positives = 14/15 (93%) Frame = +2 Query: 509 LDSGDGVSHTVPIYE 553 +DSGDGV+H VP+YE Sbjct: 153 VDSGDGVTHIVPVYE 167 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 88.6 bits (210), Expect = 6e-19 Identities = 39/77 (50%), Positives = 54/77 (70%), Gaps = 1/77 (1%) Frame = +1 Query: 262 PIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN 438 PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLN 133 Query: 439 TPAMYVAIQAVLSLYAS 489 PA+ ++Q VL+LYAS Sbjct: 134 VPALSFSLQPVLALYAS 150 Score = 76.6 bits (180), Expect = 3e-15 Identities = 34/63 (53%), Positives = 44/63 (69%) Frame = +2 Query: 77 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTP 256 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71 Query: 257 NTP 265 P Sbjct: 72 QRP 74 Score = 35.9 bits (79), Expect = 0.005 Identities = 16/37 (43%), Positives = 23/37 (62%) Frame = +2 Query: 509 LDSGDGVSHTVPIYEGYRTPPRHSASGT*PGGDLTDY 619 L+ GDG++H+VPIY+G+ P G D+TDY Sbjct: 158 LECGDGLTHSVPIYDGFSIPSAIQQEEI-GGRDVTDY 193 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 66.5 bits (155), Expect = 3e-12 Identities = 31/85 (36%), Positives = 50/85 (58%) Frame = +1 Query: 262 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 441 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 442 PAMYVAIQAVLSLYASVVPPVSSGL 516 P++ I + S Y + P SSG+ Sbjct: 145 PSVAYGIDGLFSFYHN-TKPSSSGI 168 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 77 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 172 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 65.7 bits (153), Expect = 5e-12 Identities = 31/78 (39%), Positives = 43/78 (55%) Frame = +1 Query: 256 KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 435 K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MFE+ Sbjct: 66 KNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESL 125 Query: 436 NTPAMYVAIQAVLSLYAS 489 PA Y+A Q + +AS Sbjct: 126 RCPATYLAKQETCAAFAS 143 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 62 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 163 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 53.2 bits (122), Expect = 3e-08 Identities = 22/73 (30%), Positives = 42/73 (57%) Frame = +1 Query: 271 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 450 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 451 YVAIQAVLSLYAS 489 Y+ QAV +A+ Sbjct: 134 YLTKQAVCVAFAN 146 Score = 38.7 bits (86), Expect = 7e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +2 Query: 62 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 232 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 42.7 bits (96), Expect = 4e-05 Identities = 25/84 (29%), Positives = 39/84 (46%), Gaps = 4/84 (4%) Frame = +1 Query: 247 PDPKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 414 P+ PI+ G V +W+ ++ W H Y+ L P + +PV L +RE T Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166 Query: 415 QIMFETFNTPAMYVAIQAVLSLYA 486 Q FE P +A + ++ LYA Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYA 190 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 39.9 bits (89), Expect = 3e-04 Identities = 20/55 (36%), Positives = 30/55 (54%) Frame = +2 Query: 122 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTPNTPSNTESSL 286 G+ PRA F ++ P H G+++ M KD G+E S +G +P TP N S + Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTPENEISEV 554 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 32.3 bits (70), Expect = 0.058 Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 2/60 (3%) Frame = +1 Query: 322 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASVV 495 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + + A+ L V Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQHLNIQAV 1458 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.31 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -1 Query: 219 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 58 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 28.7 bits (61), Expect = 0.72 Identities = 15/40 (37%), Positives = 18/40 (45%) Frame = -2 Query: 260 YLGSGYLFCSVPHLLHKSPSVPYRPSRPDGGAFPRSRGTR 141 Y L + L+ K+PS PY SRP A S TR Sbjct: 296 YFKENGLNYDIDQLISKAPSYPYSSSRPSASASLASSPTR 335 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 2.9 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -1 Query: 222 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 106 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.8 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 364 LLTEAPLNPKANREKMTQIMFETFN 438 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr 3|||Manual Length = 1647 Score = 26.2 bits (55), Expect = 3.8 Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = +3 Query: 489 GRTTGIVWTPATVSPTPCPSTKDTALPHAILRLG-LSPAATSQTT 620 G T + T TVS T S +T LPHA+ R+G L P ++T Sbjct: 1331 GVTVHNLGTQGTVSVTS--SVIETILPHALRRIGALKPEELGKST 1373 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 5.1 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 104 TYRSHCLLPTRQLLRRTS*ISEI 36 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.8 bits (54), Expect = 5.1 Identities = 13/23 (56%), Positives = 13/23 (56%) Frame = +3 Query: 495 TTGIVWTPATVSPTPCPSTKDTA 563 TT TPA V P P PST TA Sbjct: 838 TTSSHITPADVHPLPPPSTSTTA 860 >SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 25.8 bits (54), Expect = 5.1 Identities = 9/34 (26%), Positives = 19/34 (55%) Frame = +1 Query: 250 DPKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE 351 +P YP G++ +W ++E + E+R+ P+ Sbjct: 241 NPFYPRTDGLLFSWSELESMNSENMKPEIRLIPK 274 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 25.0 bits (52), Expect = 8.8 Identities = 9/26 (34%), Positives = 15/26 (57%) Frame = +2 Query: 497 HRYRLDSGDGVSHTVPIYEGYRTPPR 574 H+ + S V+H +P+ G +PPR Sbjct: 975 HQEQTTSTTKVAHPIPVSNGLSSPPR 1000 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,943,735 Number of Sequences: 5004 Number of extensions: 67783 Number of successful extensions: 224 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 220 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 273658928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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