BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0113 (725 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 167 1e-42 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 80 4e-16 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 76 6e-15 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 72 7e-14 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 59 6e-10 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 52 1e-07 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 46 4e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 46 4e-06 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 42 9e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.6 SPBC13E7.08c |||RNA polymerase II associated Paf1 complex |Schiz... 27 2.7 SPBC8D2.07c |sfc9||transcription factor TFIIIC complex subunit S... 26 4.8 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 26 4.8 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 6.3 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 6.3 SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe... 26 6.3 SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosacchar... 25 8.3 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 167 bits (406), Expect = 1e-42 Identities = 88/134 (65%), Positives = 100/134 (74%), Gaps = 3/134 (2%) Frame = -3 Query: 654 KVITIGKRKDSGCPEALFPTLVLGYGSLRHPRDHI*LHHEVRRGHP*---GLYANTVLSG 484 +VITIG + PEALF LG L + H ++ + + LY N V+SG Sbjct: 246 QVITIGNERFRA-PEALFQPSALG---LENAGIHEATYNSIMKCDVDIRKDLYGNVVMSG 301 Query: 483 GTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQE 304 GTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQE Sbjct: 302 GTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQE 361 Query: 303 YDESGPSIVHRKCF 262 YDESGP IV+RKCF Sbjct: 362 YDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 79.8 bits (188), Expect = 4e-16 Identities = 36/83 (43%), Positives = 59/83 (71%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTF 331 LY+N VLSGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF Sbjct: 298 LYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTF 357 Query: 330 QQMWISKQEYDESGPSIVHRKCF 262 +++ I+ +EY ++ +++ R+ F Sbjct: 358 RRLLITSEEY-KNDQNVIFRRRF 379 Score = 33.5 bits (73), Expect = 0.031 Identities = 13/34 (38%), Positives = 20/34 (58%) Frame = -1 Query: 617 AQRLSFQPSFLGMEACGIHETTYNSIMKCDVDIR 516 A + F P +G EA G+H + SI+ D+D+R Sbjct: 262 APEVLFNPEMMGSEASGLHIQLFKSILLSDIDLR 295 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 75.8 bits (178), Expect = 6e-15 Identities = 37/88 (42%), Positives = 59/88 (67%), Gaps = 6/88 (6%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASL 340 L N ++ GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL Sbjct: 346 LLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSL 404 Query: 339 STFQQMWISKQEYDESGP---SIVHRKC 265 TF Q+WIS+QEY+E G +++ ++C Sbjct: 405 GTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 72.1 bits (169), Expect = 7e-14 Identities = 35/76 (46%), Positives = 49/76 (64%), Gaps = 2/76 (2%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSILASLS 337 L N V++GGT++ PG+++R+Q E+ LA S + + +VW GGSILASL Sbjct: 343 LLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLD 402 Query: 336 TFQQMWISKQEYDESG 289 FQ +W+SKQEYDE G Sbjct: 403 NFQHLWVSKQEYDEVG 418 Score = 31.9 bits (69), Expect = 0.096 Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 9/60 (15%) Frame = -1 Query: 668 FPDGSRSSLSGNEKIPVAQRLSFQPSFLGMEAC---------GIHETTYNSIMKCDVDIR 516 FPDG G E+ +++ L F PSF + G+HE Y SI+ CD ++R Sbjct: 283 FPDGVTHKF-GQERFRISEIL-FNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELR 340 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 59.3 bits (137), Expect = 6e-10 Identities = 34/86 (39%), Positives = 53/86 (61%), Gaps = 12/86 (13%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWI 364 LY VLSGG++MY G+ R++KEI L P+ + K+KI P R+++V+I Sbjct: 293 LYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFI 352 Query: 363 GGSILAS-LSTFQQMWISKQEYDESG 289 GG++LA ++ MW+SK E++E G Sbjct: 353 GGAVLADIMAQNDHMWVSKAEWEEYG 378 Score = 36.7 bits (81), Expect = 0.003 Identities = 23/57 (40%), Positives = 33/57 (57%), Gaps = 2/57 (3%) Frame = -1 Query: 677 LRNF--PDGSRSSLSGNEKIPVAQRLSFQPSFLGMEACGIHETTYNSIMKCDVDIRK 513 +RN+ PDG R G+E+ + L FQP +G E G+ E +++I DVDIRK Sbjct: 237 MRNYTLPDG-RVIKVGSERYECPECL-FQPHLVGSEQPGLSEFIFDTIQAADVDIRK 291 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 51.6 bits (118), Expect = 1e-07 Identities = 28/95 (29%), Positives = 48/95 (50%), Gaps = 14/95 (14%) Frame = -3 Query: 513 GLYANTVLSGGTTMYPGIADRMQKEITALAPSTMK--------------IKIIAPPERKY 376 GLY N VLSGG+T++ +R+Q+++ + + + +I+ ++ Sbjct: 325 GLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRN 384 Query: 375 SVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 271 +VW GGS+LA F +K +Y+E G SI R Sbjct: 385 AVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 46.4 bits (105), Expect = 4e-06 Identities = 25/81 (30%), Positives = 40/81 (49%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTF 331 L N V GG +PG R+ E+ +LAP+ ++K+ P + W S + L + Sbjct: 319 LLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHW 377 Query: 330 QQMWISKQEYDESGPSIVHRK 268 I++ EY E G +I+ RK Sbjct: 378 NANKITRSEYSEHGANIMTRK 398 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 46.4 bits (105), Expect = 4e-06 Identities = 20/78 (25%), Positives = 39/78 (50%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTF 331 L +N +++GG PG+ R+++E+T++ P I + W G S + F Sbjct: 634 LVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKF 693 Query: 330 QQMWISKQEYDESGPSIV 277 + ++++EY E GP + Sbjct: 694 KAAKVTREEYLEKGPEYI 711 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 41.9 bits (94), Expect = 9e-05 Identities = 19/84 (22%), Positives = 40/84 (47%), Gaps = 3/84 (3%) Frame = -3 Query: 510 LYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASL 340 LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I + Sbjct: 529 LYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRI 588 Query: 339 STFQQMWISKQEYDESGPSIVHRK 268 ++WI ++ G ++ K Sbjct: 589 RIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.6 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = -3 Query: 369 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 268 ++GGSI+A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPBC13E7.08c |||RNA polymerase II associated Paf1 complex |Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 27.1 bits (57), Expect = 2.7 Identities = 14/44 (31%), Positives = 22/44 (50%) Frame = -1 Query: 716 RIGVPGAPPSKKSLRNFPDGSRSSLSGNEKIPVAQRLSFQPSFL 585 R+G G+ S + + DGS S GN+ +L QP+F+ Sbjct: 141 RLGPSGSYQSNAQIVQWSDGSYSLRIGNDIYDTQNKLISQPTFV 184 >SPBC8D2.07c |sfc9||transcription factor TFIIIC complex subunit Sfc9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 26.2 bits (55), Expect = 4.8 Identities = 9/29 (31%), Positives = 16/29 (55%) Frame = +1 Query: 538 MMELYVVSWMPQASIPKNEGWKESLWATG 624 + ++ V+W P S+P + W L+A G Sbjct: 141 LCRIHSVAWSPLVSLPSSSPWGVCLFALG 169 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 26.2 bits (55), Expect = 4.8 Identities = 9/28 (32%), Positives = 14/28 (50%) Frame = -2 Query: 706 YPGPPPQKSLYGTFPTGQGHHYRETKRF 623 + PP + Y T+ G GHH + R+ Sbjct: 142 FSAPPRRSRGYNTYGQGSGHHPMDKSRY 169 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -1 Query: 377 TPYGSVDRSSPPSLPSNRCGSRNRSTTSLAPP 282 TP + PPS+PS+R R S ++ APP Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -3 Query: 444 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 334 KEI++ A S + + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/33 (39%), Positives = 22/33 (66%), Gaps = 2/33 (6%) Frame = -3 Query: 660 RVKVITI-GKRKDSGCPEALFPTLVL-GYGSLR 568 ++K + I GK+ PEA+ PTL++ G+G L+ Sbjct: 138 QIKFVKIPGKQAVENYPEAMMPTLLIYGHGDLQ 170 >SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.4 bits (53), Expect = 8.3 Identities = 17/62 (27%), Positives = 30/62 (48%) Frame = -2 Query: 487 RWYHHVPWNRRPYAKGNHSSRPIDNED*DHCSSREEVLRMDRWIDPRLPLYLPTDVDLET 308 RWY PW + KG+ + +E + SSR + + W+D YL +++D+ + Sbjct: 483 RWYE--PW----FVKGDLN---YTSEVKERTSSRLSAVGSENWVDDDFYEYLLSEIDMVS 533 Query: 307 GV 302 V Sbjct: 534 AV 535 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,031,828 Number of Sequences: 5004 Number of extensions: 63009 Number of successful extensions: 198 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 182 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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