BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0103 (646 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse... 46 3e-05 At4g26990.1 68417.m03883 expressed protein 29 2.6 At5g09670.2 68418.m01119 loricrin-related contains weak similari... 28 6.1 At5g09670.1 68418.m01118 loricrin-related contains weak similari... 28 6.1 At4g19900.1 68417.m02916 glycosyl transferase-related contains P... 27 8.1 >At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-consensus AT donor splice site at exon 6, AC acceptor splice site at exon 7; identical to Swiss-Prot:P46416 glutathione synthetase, chloroplast precursor (Glutathione synthase) (GSH synthetase) (GSH-S) [Arabidopsis thaliana] Length = 478 Score = 45.6 bits (103), Expect = 3e-05 Identities = 19/32 (59%), Positives = 23/32 (71%) Frame = +3 Query: 189 FVLKPQREGGGNNVYGSEVRDA*LACDTAGSE 284 FV+KPQREGGGNN+YG +VR+ L G E Sbjct: 364 FVMKPQREGGGNNIYGDDVRENLLRLQKEGEE 395 >At4g26990.1 68417.m03883 expressed protein Length = 474 Score = 29.1 bits (62), Expect = 2.6 Identities = 16/40 (40%), Positives = 21/40 (52%) Frame = -3 Query: 323 PEAAISSPSVCTRLAPGCVACESRVPHFRTVHIIPATFPL 204 PEA I SPS RL+P V +PH + IP+ P+ Sbjct: 256 PEAKIFSPSYTKRLSPSPVG----MPHVGNIAYIPSNTPM 291 >At5g09670.2 68418.m01119 loricrin-related contains weak similarity to Loricrin (Swiss-Prot:P23490) [Homo sapiens] Length = 546 Score = 27.9 bits (59), Expect = 6.1 Identities = 11/30 (36%), Positives = 13/30 (43%) Frame = +3 Query: 492 GEGINKTSCNKNQTRKVIFCVITGGRTSCD 581 G+ K CNK K FC GG C+ Sbjct: 178 GQRCQKPGCNKGAESKTTFCKTHGGGKRCE 207 >At5g09670.1 68418.m01118 loricrin-related contains weak similarity to Loricrin (Swiss-Prot:P23490) [Homo sapiens] Length = 546 Score = 27.9 bits (59), Expect = 6.1 Identities = 11/30 (36%), Positives = 13/30 (43%) Frame = +3 Query: 492 GEGINKTSCNKNQTRKVIFCVITGGRTSCD 581 G+ K CNK K FC GG C+ Sbjct: 178 GQRCQKPGCNKGAESKTTFCKTHGGGKRCE 207 >At4g19900.1 68417.m02916 glycosyl transferase-related contains Pfam profiles PF01535: PPR repeat, PF04572: Alpha 1,4-glycosyltransferase conserved region, PF04488: Glycosyltransferase sugar-binding region containing DXD motif; several hypothetical proteins - Arabidopsis thaliana Length = 1302 Score = 27.5 bits (58), Expect = 8.1 Identities = 15/53 (28%), Positives = 27/53 (50%) Frame = -3 Query: 344 ELNDRSLPEAAISSPSVCTRLAPGCVACESRVPHFRTVHIIPATFPLRLQYES 186 E +D SLP + SSPS C + + C S + R H++ + + L +++ Sbjct: 638 ESSDLSLPSSPSSSPSQCLVKSVCSLVCTS---YLRQNHVVSSPHRVNLDFDA 687 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,149,293 Number of Sequences: 28952 Number of extensions: 313059 Number of successful extensions: 666 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 649 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 666 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1334473344 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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