BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0081 (553 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 142 2e-35 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 79 4e-16 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 75 5e-15 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 64 1e-11 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 59 5e-10 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 55 8e-09 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 47 2e-06 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.065 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.26 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 28 0.80 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 0.80 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 1.8 SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 1.8 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 27 2.4 SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa... 26 3.2 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.2 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 4.2 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 25 5.6 SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 7.4 SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe... 25 7.4 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 7.4 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 7.4 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 9.8 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 142 bits (345), Expect = 2e-35 Identities = 64/71 (90%), Positives = 69/71 (97%) Frame = +2 Query: 44 DEEVAALVVDNGSGMCKAGFAGDDAPCAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 223 +EE+AALV+DNGSGMCKAGFAGDDAP AVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 224 RGILTLKYPID 256 RGILTLKYPI+ Sbjct: 62 RGILTLKYPIE 72 Score = 125 bits (301), Expect = 5e-30 Identities = 53/64 (82%), Positives = 56/64 (87%) Frame = +1 Query: 247 PHRHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEASLNPKANREKMTHIMFQTFNT 426 P HGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEA LNPK+NREKMT I+F+TFN Sbjct: 70 PIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNA 129 Query: 427 PAMY 438 PA Y Sbjct: 130 PAFY 133 Score = 46.4 bits (105), Expect = 3e-06 Identities = 23/32 (71%), Positives = 24/32 (75%) Frame = +3 Query: 456 VLSLYASGRTTGIVLDSRTVSSTPCPFYEGYA 551 VLSLYASGRTTGIVLDS + P YEGYA Sbjct: 139 VLSLYASGRTTGIVLDSGDGVTHTVPIYEGYA 170 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 79.0 bits (186), Expect = 4e-16 Identities = 35/65 (53%), Positives = 47/65 (72%) Frame = +2 Query: 62 LVVDNGSGMCKAGFAGDDAPCAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 241 + +DNGSG KAGFAGDD P +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71 Query: 242 KYPID 256 + PI+ Sbjct: 72 QRPIE 76 Score = 66.5 bits (155), Expect = 2e-12 Identities = 29/66 (43%), Positives = 41/66 (62%), Gaps = 1/66 (1%) Frame = +1 Query: 241 QIPHRHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEASLNPKANREKMTHIMFQT 417 Q P GI+ NW DME+IW + + ++ L PEEHP+LLTE L N+EK+ ++T Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131 Query: 418 FNTPAM 435 N PA+ Sbjct: 132 LNVPAL 137 Score = 38.3 bits (85), Expect = 7e-04 Identities = 17/34 (50%), Positives = 24/34 (70%) Frame = +3 Query: 450 KPVLSLYASGRTTGIVLDSRTVSSTPCPFYEGYA 551 +PVL+LYAS RTTGIVL+ + P Y+G++ Sbjct: 142 QPVLALYASARTTGIVLECGDGLTHSVPIYDGFS 175 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 75.4 bits (177), Expect = 5e-15 Identities = 31/64 (48%), Positives = 39/64 (60%) Frame = +1 Query: 247 PHRHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEASLNPKANREKMTHIMFQTFNT 426 P RHG + NWD ME+ W + + LR PE+H LLTE LNP NRE IMF++FN Sbjct: 93 PIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNC 152 Query: 427 PAMY 438 +Y Sbjct: 153 AGLY 156 Score = 42.7 bits (96), Expect = 3e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +2 Query: 62 LVVDNGSGMCKAGFAGDDAPCAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 211 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 64.1 bits (149), Expect = 1e-11 Identities = 24/64 (37%), Positives = 42/64 (65%) Frame = +1 Query: 247 PHRHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEASLNPKANREKMTHIMFQTFNT 426 P +GI+ ++++M ++W +TF+ +L++ P +LLTE +NP ANREKM MF+ + Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129 Query: 427 PAMY 438 +Y Sbjct: 130 GGVY 133 Score = 63.7 bits (148), Expect = 2e-11 Identities = 33/71 (46%), Positives = 46/71 (64%), Gaps = 2/71 (2%) Frame = +2 Query: 50 EVAALVVDNGSGMCKAGFAGDDAPCAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 223 E A +V+DNG+G K G+A D+ P FPSIVGRP R + + KD VGDEA++ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61 Query: 224 RGILTLKYPID 256 R +L +KYP++ Sbjct: 62 RSLLQVKYPME 72 Score = 29.1 bits (62), Expect = 0.46 Identities = 14/29 (48%), Positives = 19/29 (65%) Frame = +3 Query: 456 VLSLYASGRTTGIVLDSRTVSSTPCPFYE 542 VLSLYA G ++G+V+DS + P YE Sbjct: 139 VLSLYAQGLSSGVVVDSGDGVTHIVPVYE 167 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 58.8 bits (136), Expect = 5e-10 Identities = 26/70 (37%), Positives = 36/70 (51%) Frame = +1 Query: 253 RHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEASLNPKANREKMTHIMFQTFNTPA 432 R+G V NWD +W + +L+ P EHP+L+TE NP NR K MF++ PA Sbjct: 70 RNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPA 129 Query: 433 MYGGHPSPCS 462 Y C+ Sbjct: 130 TYLAKQETCA 139 Score = 38.3 bits (85), Expect = 7e-04 Identities = 16/34 (47%), Positives = 23/34 (67%) Frame = +2 Query: 47 EEVAALVVDNGSGMCKAGFAGDDAPCAVFPSIVG 148 +EV+A+V+D GS + GF+G+D P V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 54.8 bits (126), Expect = 8e-09 Identities = 24/66 (36%), Positives = 40/66 (60%), Gaps = 1/66 (1%) Frame = +1 Query: 247 PHRHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEASLNPKANREKMTHIMFQTFNT 426 P +++NWD ME++ +TF +L + EHP+ +TE NP R MT ++F+ +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 427 PAM-YG 441 P++ YG Sbjct: 145 PSVAYG 150 Score = 33.9 bits (74), Expect = 0.016 Identities = 15/32 (46%), Positives = 21/32 (65%) Frame = +2 Query: 62 LVVDNGSGMCKAGFAGDDAPCAVFPSIVGRPR 157 LV+DNGS +AG+ G+ P VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 47.2 bits (107), Expect = 2e-06 Identities = 18/61 (29%), Positives = 35/61 (57%) Frame = +1 Query: 256 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEASLNPKANREKMTHIMFQTFNTPAM 435 +GI+ +W+ W +L+V P E+ +++TE S NP++ R+++ F+ + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 436 Y 438 Y Sbjct: 134 Y 134 Score = 38.7 bits (86), Expect = 6e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +2 Query: 47 EEVAALVVDNGSGMCKAGFAGDDAPCAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 217 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.065 Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%) Frame = +1 Query: 307 FYNELRVAPEEHPVLLTE--ASLNPKANREKMTHIMFQTFNTP 429 +Y L E+HP+LLT+ A+L P+ + ++ I ++ NTP Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.26 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -2 Query: 204 PT*ESFCPI-PTITP*WRGLPTIEGNTAQGASSPAKPALHIPEPLSTTNAATSSS 43 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 28.3 bits (60), Expect = 0.80 Identities = 18/41 (43%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -2 Query: 207 SPT*ESFC--PIPTITP*WRGLPTIEGNTAQGASSPAKPAL 91 S T +SF P+PT P LPT NT Q P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 28.3 bits (60), Expect = 0.80 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -3 Query: 215 VPHLLHKSPSVPYRPSRPDGGAFPRSRGTR 126 + L+ K+PS PY SRP A S TR Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPTR 335 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.1 bits (57), Expect = 1.8 Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 4/70 (5%) Frame = +1 Query: 232 PDPQIPHRHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEASLNPKANREKMT 399 P+ P + G V +W+ ++ W H Y+ L P + +PV L + +RE T Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166 Query: 400 HIMFQTFNTP 429 F+ P Sbjct: 167 QFFFEECQVP 176 >SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 127 Score = 27.1 bits (57), Expect = 1.8 Identities = 23/60 (38%), Positives = 28/60 (46%) Frame = -1 Query: 244 FEGQDTSFALCLISYIRVLLSHTDHHALMAGPSHDRGEHGARSIISCETGLAHTGAIVYY 65 F G +F CLI++IR +SH D EHG II C T L G I+YY Sbjct: 47 FAGLGIAFIYCLIAFIRE-MSHPSSR-------KDTMEHGL-PIILCST-LMLVGNILYY 96 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 26.6 bits (56), Expect = 2.4 Identities = 12/47 (25%), Positives = 29/47 (61%), Gaps = 3/47 (6%) Frame = +1 Query: 244 IPHRHGIVTNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEASLNP 375 +P + ++ + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 265 VPQGNFVIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 >SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 889 Score = 26.2 bits (55), Expect = 3.2 Identities = 15/48 (31%), Positives = 22/48 (45%) Frame = +2 Query: 185 QKDSYVGDEAQSKRGILTLKYPIDTESSLTGMTWRRFGIIPSTMSCVS 328 +KD+ V E SK T YP + S LT + + + +SC S Sbjct: 143 EKDNLVNSEYSSKYSSTTRIYPYHSLSQLTDLLTDGSFYVSTNISCFS 190 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 4.2 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -3 Query: 89 TYRSHCLLPTRQLLRRTS*ISEI 21 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 25.8 bits (54), Expect = 4.2 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -1 Query: 244 FEGQDTSFALCLISYIRVLLSHTDHHA 164 +EG+DT+ +Y+R++L TD A Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 25.4 bits (53), Expect = 5.6 Identities = 9/20 (45%), Positives = 11/20 (55%) Frame = +1 Query: 457 CSRCTRPVVPPVSCWTPGRC 516 CS CT + P S W+ G C Sbjct: 197 CSVCTDTINPSTSIWSCGTC 216 >SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 25.0 bits (52), Expect = 7.4 Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = -2 Query: 231 IPLLLCASSPT*ESFCPIPTI-TP*WRGLPTIEGNTAQGASSPAKPALHIPEPLSTTNAA 55 IP L ++P +F P P++ +P + T +TA ++SP L P + TN + Sbjct: 363 IPSPLQNTNPAPSTF-PNPSVASPAFPNSSTSNPSTAPASASPLASTLK-PSTANDTNGS 420 Query: 54 TSSS 43 +SSS Sbjct: 421 SSSS 424 >SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 25.0 bits (52), Expect = 7.4 Identities = 14/27 (51%), Positives = 14/27 (51%) Frame = -2 Query: 144 TIEGNTAQGASSPAKPALHIPEPLSTT 64 T EG AQ P P LHIP P TT Sbjct: 31 TFEG--AQPQWEPPIPGLHIPPPPGTT 55 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.0 bits (52), Expect = 7.4 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = -1 Query: 490 PVVRPDAYSESTGLDGHRTWRA 425 P P A S+ T +DGHR A Sbjct: 384 PKAAPKAVSQKTNVDGHRNHEA 405 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 7.4 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 167 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 253 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 9.8 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +2 Query: 62 LVVDNGSGMCKAGFAG 109 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,674,445 Number of Sequences: 5004 Number of extensions: 61500 Number of successful extensions: 179 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 229961028 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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