BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0059 (351 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 120 9e-29 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 112 1e-26 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 71 5e-14 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 61 6e-11 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 2.0 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 26 2.0 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 2.0 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 2.6 SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|... 25 2.6 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 3.4 SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 25 4.5 SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual 24 6.0 SPAC4G8.13c |prz1||transcription factor Prz1 |Schizosaccharomyce... 24 6.0 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 120 bits (288), Expect = 9e-29 Identities = 51/75 (68%), Positives = 60/75 (80%) Frame = +2 Query: 35 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 214 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 215 HVPRAVFVDLEPTVL 259 +VPR+++VDLEP V+ Sbjct: 61 YVPRSIYVDLEPNVI 75 Score = 61.7 bits (143), Expect = 3e-11 Identities = 26/32 (81%), Positives = 29/32 (90%) Frame = +1 Query: 256 VDEVRTGTYRQLFHAEQLITGKEDAANNYARG 351 +D+VRTG YR LFH EQLITGKEDA+NNYARG Sbjct: 75 IDQVRTGPYRDLFHPEQLITGKEDASNNYARG 106 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 112 bits (270), Expect = 1e-26 Identities = 55/80 (68%), Positives = 61/80 (76%), Gaps = 5/80 (6%) Frame = +2 Query: 35 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 199 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 200 TGAGKHVPRAVFVDLEPTVL 259 TG GK VPR+++VDLEP V+ Sbjct: 60 TGQGKFVPRSIYVDLEPNVI 79 Score = 59.3 bits (137), Expect = 2e-10 Identities = 23/32 (71%), Positives = 29/32 (90%) Frame = +1 Query: 256 VDEVRTGTYRQLFHAEQLITGKEDAANNYARG 351 +D+VRTG Y+ LFH EQ++TGKEDA+NNYARG Sbjct: 79 IDQVRTGPYKDLFHPEQMVTGKEDASNNYARG 110 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 70.9 bits (166), Expect = 5e-14 Identities = 35/95 (36%), Positives = 54/95 (56%) Frame = +2 Query: 38 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 217 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 218 VPRAVFVDLEPTVLMRSALAHTDSCFMQNNLLLVR 322 +PRA+ +DLEP V+ S + N+L+ + Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITK 95 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 60.9 bits (141), Expect = 6e-11 Identities = 32/93 (34%), Positives = 45/93 (48%) Frame = +2 Query: 35 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 214 MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 215 HVPRAVFVDLEPTVLMRSALAHTDSCFMQNNLL 313 +VPRAV VDLEP + + F +N++ Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNII 91 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 2.0 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = +3 Query: 114 STASSLMARCPQTRPSGVETILSTLSSARPELAST 218 ST SSL + ++PS T ST SSA P S+ Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207 Score = 23.8 bits (49), Expect = 7.9 Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = +3 Query: 42 SASLYTLAKPESRSVMPAGSFTAWSTASSL-MARCPQTRPSGVETILSTLSSARPELAST 218 S+S + + S S P+ S ++ T+SSL + P T S T S SS+ AS+ Sbjct: 206 SSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASS 265 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.8 bits (54), Expect = 2.0 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = -3 Query: 286 VCMCQCGPHQHSRFKIDEDSTGYV 215 VC + G Q S KID + TGYV Sbjct: 1877 VCNDRSGHRQSSVIKIDPNETGYV 1900 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 2.0 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +1 Query: 133 WPDAHRQDHRGWRRFFQHFLQR 198 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 2.6 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 24 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 146 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 >SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 25.4 bits (53), Expect = 2.6 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -2 Query: 245 QDRRRQHGVRACQLRSR*RKC*KNRLHPRWSCL 147 QD++ Q R R R RK +RLHP + C+ Sbjct: 7 QDKKNQEIFRISCQRCRQRKIKCDRLHPCFQCV 39 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 25.0 bits (52), Expect = 3.4 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -3 Query: 88 ITDLDSGLANVYRDALTHFDLSF 20 +T +D GLA YRD TH + + Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168 >SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 365 Score = 24.6 bits (51), Expect = 4.5 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = -3 Query: 142 HLAIRLDAVLQAV-KLPAGITDLDSGLANVYRDALTHFDLSF 20 H I+ D L + K + +D GLA YRD TH + + Sbjct: 128 HRDIKPDNFLMGIGKRGNQVNIIDFGLAKKYRDHKTHLHIPY 169 >SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual Length = 566 Score = 24.2 bits (50), Expect = 6.0 Identities = 18/57 (31%), Positives = 25/57 (43%) Frame = -1 Query: 210 PAPVSLKKVLKESSPPPMVLSVGIWPSGWMPCSRQ*SSQQALPIWTPAWPTCTEMHS 40 P P+ LK + E P L V IW P + +PI TPA+P+ H+ Sbjct: 268 PQPILLKPI--EDGP----LQVRIWNPKLYPSDKA----HRMPIITPAYPSMCATHN 314 >SPAC4G8.13c |prz1||transcription factor Prz1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 681 Score = 24.2 bits (50), Expect = 6.0 Identities = 14/40 (35%), Positives = 19/40 (47%) Frame = +2 Query: 176 SFNTFFSETGAGKHVPRAVFVDLEPTVLMRSALAHTDSCF 295 S N+ FSE A + VF + PT L S L + + F Sbjct: 367 SVNSPFSEDHADASLTTHVFDPISPTALSNSVLNYDSNNF 406 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,612,860 Number of Sequences: 5004 Number of extensions: 32430 Number of successful extensions: 117 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 114 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 106195544 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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