BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0058 (629 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 197 9e-52 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 100 3e-22 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 94 1e-20 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 68 1e-12 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 67 2e-12 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 1e-08 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 53 3e-08 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.10 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.73 SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizos... 28 1.3 SPAC1399.05c |||transcription factor, zf-fungal binuclear cluste... 28 1.3 SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 27 3.0 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 3.0 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.9 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.9 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.2 SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 26 5.2 SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces po... 25 9.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 197 bits (481), Expect = 9e-52 Identities = 99/162 (61%), Positives = 112/162 (69%) Frame = +1 Query: 115 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGRRTLM*EMRHRAK 294 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMG++ ++K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 295 XXXXXXXXXXXXXXXLTGMTWRRFWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 474 + WHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 475 IMFETFNTPAMSLASKPVLSLYAFRSFHRYRAWTSGDGVFHT 600 I+FETFN PA +A + VLSLYA SGDGV HT Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGI-VLDSGDGVTHT 162 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 99.5 bits (237), Expect = 3e-22 Identities = 50/138 (36%), Positives = 74/138 (53%), Gaps = 1/138 (0%) Frame = +1 Query: 133 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGRRTLM*EMRHRAKXXXXXX 312 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM ++ + + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71 Query: 313 XXXXXXXXXLTGMTWRRFWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 489 W + + ++ L PEEHP+LLTE PL N+EK+ + +ET Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131 Query: 490 FNTPAMSLASKPVLSLYA 543 N PA+S + +PVL+LYA Sbjct: 132 LNVPALSFSLQPVLALYA 149 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 94.3 bits (224), Expect = 1e-20 Identities = 58/161 (36%), Positives = 81/161 (50%), Gaps = 2/161 (1%) Frame = +1 Query: 121 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGRRTLM*EMRHRAK 294 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + + +M A Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61 Query: 295 XXXXXXXXXXXXXXXLTGMTWRRFWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 474 + W +TF+ +L++ P +LLTE P+NP ANREKM + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 475 IMFETFNTPAMSLASKPVLSLYAFRSFHRYRAWTSGDGVFH 597 MFE + + +A + VLSLYA + SGDGV H Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYA-QGLSSGVVVDSGDGVTH 161 Score = 44.8 bits (101), Expect = 1e-05 Identities = 20/58 (34%), Positives = 35/58 (60%) Frame = +3 Query: 237 RDGRYGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKILASYLLQ*AACRPRG 410 + G KD VGDEA++ R +L +KYP+E+GI+ ++++M ++ + PRG Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRG 100 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 67.7 bits (158), Expect = 1e-12 Identities = 38/86 (44%), Positives = 50/86 (58%), Gaps = 7/86 (8%) Frame = +1 Query: 361 RFWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMSLASKPVLSLY 540 RFW + + LR PE+H LLTE PLNP NRE +IMFE+FN + +A + VL+L Sbjct: 107 RFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALA 166 Query: 541 AFRSFHRY--RAWT-----SGDGVFH 597 A + + R+ T SGDGV H Sbjct: 167 ASWTSSKVTDRSLTGTVVDSGDGVTH 192 Score = 40.7 bits (91), Expect = 2e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +1 Query: 133 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 216 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Frame = +3 Query: 261 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKILASYLLQ*AACRP 404 D ++G++A K +L YPI HG + NWD ME+ L + C P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEP 121 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 67.3 bits (157), Expect = 2e-12 Identities = 43/142 (30%), Positives = 65/142 (45%) Frame = +1 Query: 118 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGRRTLM*EMRHRAKX 297 +EV+A+V+D GS + GF+G+D P+ V PS G GRR E +++ Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGE-------FSDGRRLFGEEYIYKSNP 61 Query: 298 XXXXXXXXXXXXXXLTGMTWRRFWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 477 +T W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 GMEIKNAIRNGWVENWDVTVD-LWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 478 MFETFNTPAMSLASKPVLSLYA 543 MFE+ PA LA + + +A Sbjct: 121 MFESLRCPATYLAKQETCAAFA 142 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 54.4 bits (125), Expect = 1e-08 Identities = 39/139 (28%), Positives = 67/139 (48%), Gaps = 3/139 (2%) Frame = +1 Query: 133 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM---VGMGRRTLM*EMRHRAKXXX 303 LV+DNGS +AG+ G+ P+ VF ++V R R + + +G TL+ E+ R+ Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRKLSRTSTLVGNDTLI-EVGSRSIARS 85 Query: 304 XXXXXXXXXXXXLTGMTWRRFWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 483 + + +TF +L + EHP+ +TE NP R MT+++F Sbjct: 86 PFERNVISNWDLM-----EQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLF 139 Query: 484 ETFNTPAMSLASKPVLSLY 540 E +N P+++ + S Y Sbjct: 140 ELYNAPSVAYGIDGLFSFY 158 Score = 31.1 bits (67), Expect = 0.14 Identities = 13/45 (28%), Positives = 27/45 (60%), Gaps = 1/45 (2%) Frame = +3 Query: 237 RDGRYGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIL 368 RD + + + VG++ + G ++ + P E +++NWD ME++L Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVL 102 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 53.2 bits (122), Expect = 3e-08 Identities = 34/142 (23%), Positives = 65/142 (45%) Frame = +1 Query: 118 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGRRTLM*EMRHRAKX 297 EE+ +LV+D GS + G+AG+++P + PS G + + G + + E++ A Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYGV---RSDVTGRNKYVVD-ELQIHAPI 63 Query: 298 XXXXXXXXXXXXXXLTGMTWRRFWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 477 + W +L+V P E+ +++TE NP++ R+++ + Sbjct: 64 PGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEA 123 Query: 478 MFETFNTPAMSLASKPVLSLYA 543 FE + PA L + V +A Sbjct: 124 AFEQLHVPAFYLTKQAVCVAFA 145 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.5 bits (68), Expect = 0.10 Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%) Frame = +1 Query: 379 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTP 501 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 28.7 bits (61), Expect = 0.73 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -1 Query: 350 IPVSDDSVFDGVFEGQDTSFALCLISYIRVLLPIPTITP*WRGLPTIEGNTARGASSPAK 171 +P + +V +TSFAL S L PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 170 PALHIP 153 P L+ P Sbjct: 123 PQLNSP 128 >SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 282 Score = 27.9 bits (59), Expect = 1.3 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -3 Query: 288 SVPHLLHKSPSAHTDHHALMAGPSHDRGKH 199 +VP L P+ HT H + G +H++G H Sbjct: 148 TVPLLSEMDPAEHTRHDSKTLGLNHNQGAH 177 >SPAC1399.05c |||transcription factor, zf-fungal binuclear cluster type|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/50 (26%), Positives = 22/50 (44%), Gaps = 3/50 (6%) Frame = -1 Query: 614 RRWARVWKTPSPE---VQARYRWNDRNAYSESTGLDANDMAGVLNVSNMI 474 + W +W+ P P+ + Y +N S T D ND A + +M+ Sbjct: 347 KEWCHLWEQPEPQYSRIAYFYSYNVVYEASIQTATDGNDFANIPRYVSMV 396 >SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 772 Score = 26.6 bits (56), Expect = 3.0 Identities = 16/77 (20%), Positives = 33/77 (42%) Frame = +1 Query: 394 RVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMSLASKPVLSLYAFRSFHRYRAW 573 R++P LL L+ + + + + + ++ + + SL S ++ +W Sbjct: 184 RLSPSSLVPLLKLPALDHAVSSSSSSDLPSDPNSASYIASSKQKASSLKLTSSLKKFYSW 243 Query: 574 TSGDGVFHTRAHLRRDT 624 TS + HTR +L T Sbjct: 244 TSSSSLQHTRENLHDST 260 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 3.0 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -1 Query: 248 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 114 PT+ P P N S P PL+TTN TS+S Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 421 LLTEAPLNPKANREKMTQIMFETFN 495 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.9 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -1 Query: 254 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 162 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 160 TYRSHCLLPTRQLLRRTS*ISEI 92 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein 3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1385 Score = 25.8 bits (54), Expect = 5.2 Identities = 9/16 (56%), Positives = 13/16 (81%) Frame = -3 Query: 585 VAGSPSTIPVERPERV 538 V+GSPS P+ +PE+V Sbjct: 755 VSGSPSETPISKPEKV 770 >SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces pombe|chr 3|||Manual Length = 218 Score = 25.0 bits (52), Expect = 9.0 Identities = 8/24 (33%), Positives = 12/24 (50%) Frame = -1 Query: 599 VWKTPSPEVQARYRWNDRNAYSES 528 +W PE++A WN +ES Sbjct: 175 IWAISEPELEAEIAWNTTQGITES 198 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,853,013 Number of Sequences: 5004 Number of extensions: 61656 Number of successful extensions: 180 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 159 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 176 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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