BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0026 (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 182 4e-47 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 103 2e-23 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 101 6e-23 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 95 6e-21 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 69 4e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 64 1e-11 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 53 3e-08 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 44 1e-05 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.059 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.14 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 0.97 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 27 2.2 SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 2.2 SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 2.2 SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 27 3.0 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.0 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.9 SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 26 3.9 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.2 SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces ... 25 6.8 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 9.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 182 bits (443), Expect = 4e-47 Identities = 86/112 (76%), Positives = 91/112 (81%) Frame = +3 Query: 252 QRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 431 +RGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT Sbjct: 61 KRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMT 120 Query: 432 QIMFETFNTPAMYVAIQAVLSLYASGVPPVSCWTSATVSSTPCPFTKGYALP 587 QI+FETFN PA YVAIQAVLSLYASG S + P +GYALP Sbjct: 121 QIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALP 172 Score = 124 bits (300), Expect = 8e-30 Identities = 55/60 (91%), Positives = 59/60 (98%) Frame = +1 Query: 76 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 255 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 103 bits (248), Expect = 2e-23 Identities = 43/86 (50%), Positives = 65/86 (75%) Frame = +3 Query: 255 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 434 R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 435 IMFETFNTPAMYVAIQAVLSLYASGV 512 MFE + +YVAIQAVLSLYA G+ Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQGL 147 Score = 54.4 bits (125), Expect = 1e-08 Identities = 29/59 (49%), Positives = 38/59 (64%), Gaps = 2/59 (3%) Frame = +1 Query: 82 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQS 252 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60 Score = 34.3 bits (75), Expect = 0.015 Identities = 12/22 (54%), Positives = 18/22 (81%) Frame = +2 Query: 512 TTGIVLDFGDGVFHTVPIYERI 577 ++G+V+D GDGV H VP+YE + Sbjct: 148 SSGVVVDSGDGVTHIVPVYESV 169 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 101 bits (243), Expect = 6e-23 Identities = 47/94 (50%), Positives = 61/94 (64%) Frame = +3 Query: 267 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 446 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 447 TFNTPAMYVAIQAVLSLYASGVPPVSCWTSATVS 548 +FN +Y+A+QAVL+L AS WTS+ V+ Sbjct: 149 SFNCAGLYIAVQAVLALAAS-------WTSSKVT 175 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 94 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 243 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 Score = 30.3 bits (65), Expect = 0.24 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +2 Query: 506 RRTTGIVLDFGDGVFHTVPIYE 571 R TG V+D GDGV H +P+ E Sbjct: 177 RSLTGTVVDSGDGVTHIIPVAE 198 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 95.5 bits (227), Expect = 6e-21 Identities = 42/85 (49%), Positives = 60/85 (70%), Gaps = 1/85 (1%) Frame = +3 Query: 255 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMT 431 RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIA 125 Query: 432 QIMFETFNTPAMYVAIQAVLSLYAS 506 + +ET N PA+ ++Q VL+LYAS Sbjct: 126 EYFYETLNVPALSFSLQPVLALYAS 150 Score = 69.7 bits (163), Expect = 3e-13 Identities = 30/53 (56%), Positives = 39/53 (73%) Frame = +1 Query: 94 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 252 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQN 64 Score = 37.5 bits (83), Expect = 0.002 Identities = 15/21 (71%), Positives = 19/21 (90%) Frame = +2 Query: 509 RTTGIVLDFGDGVFHTVPIYE 571 RTTGIVL+ GDG+ H+VPIY+ Sbjct: 152 RTTGIVLECGDGLTHSVPIYD 172 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 69.3 bits (162), Expect = 4e-13 Identities = 32/82 (39%), Positives = 46/82 (56%) Frame = +3 Query: 264 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 443 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 444 ETFNTPAMYVAIQAVLSLYASG 509 E+ PA Y+A Q + +ASG Sbjct: 123 ESLRCPATYLAKQETCAAFASG 144 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +1 Query: 79 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 180 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 64.5 bits (150), Expect = 1e-11 Identities = 29/82 (35%), Positives = 47/82 (57%) Frame = +3 Query: 279 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 458 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 459 PAMYVAIQAVLSLYASGVPPVS 524 P++ I + S Y + P S Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSS 166 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +1 Query: 94 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 189 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 53.2 bits (122), Expect = 3e-08 Identities = 22/73 (30%), Positives = 42/73 (57%) Frame = +3 Query: 288 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 467 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 468 YVAIQAVLSLYAS 506 Y+ QAV +A+ Sbjct: 134 YLTKQAVCVAFAN 146 Score = 38.7 bits (86), Expect = 7e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +1 Query: 79 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 249 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 44.4 bits (100), Expect = 1e-05 Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 4/82 (4%) Frame = +3 Query: 279 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 446 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 447 TFNTPAMYVAIQAVLSLYASGV 512 P +A + ++ LYA G+ Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGI 193 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 32.3 bits (70), Expect = 0.059 Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 2/60 (3%) Frame = +3 Query: 339 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGV 512 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + + A+ L V Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQHLNIQAV 1458 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 31.1 bits (67), Expect = 0.14 Identities = 23/67 (34%), Positives = 28/67 (41%), Gaps = 5/67 (7%) Frame = -2 Query: 260 TSLLCASS----PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTT 96 TS C +S PT S P+ PT+ P P N S P PL+TT Sbjct: 389 TSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATT 448 Query: 95 NAATSSS 75 N TS+S Sbjct: 449 NCTTSTS 455 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 28.3 bits (60), Expect = 0.97 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -3 Query: 247 VPHLLHKSPSVPYRPSRPDGGAFPRSRGTR 158 + L+ K+PS PY SRP A S TR Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPTR 335 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 27.1 bits (57), Expect = 2.2 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +1 Query: 139 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 252 G+ PRA F ++ P H G+++ M KD G+E S Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMS 537 >SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 601 Score = 27.1 bits (57), Expect = 2.2 Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = -3 Query: 577 YPFVNGHGVEDTVAEVQHDTGGTPDA-YSESTAWMATYMAGVLNVSNMIWVIF 422 + +N GV+DT+ + + G P Y EST + A + N++ + W ++ Sbjct: 395 FEVMNCKGVQDTLTAIWNTNSGKPSGWYLESTMYKALDVT-YYNLNTLSWNLY 446 >SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 127 Score = 27.1 bits (57), Expect = 2.2 Identities = 23/58 (39%), Positives = 27/58 (46%) Frame = -1 Query: 270 GSGYLFALCLISYIRVLLSHTDHHALMAGPSHDRGEHGARSIISCETGLAHTGAIVYY 97 G G F CLI++IR +SH D EHG II C T L G I+YY Sbjct: 49 GLGIAFIYCLIAFIRE-MSHPSSR-------KDTMEHGL-PIILCST-LMLVGNILYY 96 >SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 26.6 bits (56), Expect = 3.0 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = -3 Query: 292 PCSMGYLRVRIPLCSVPHLLHKSPSVPYRP 203 P + Y PL +VP +L PSV Y P Sbjct: 349 PLQLSYAHYATPLPAVPTVLSGLPSVAYTP 378 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 3.0 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -2 Query: 239 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 123 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 381 LLTEAPLNPKANREKMTQIMFETFN 455 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 709 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/20 (55%), Positives = 16/20 (80%) Frame = +3 Query: 498 YASGVPPVSCWTSATVSSTP 557 +++GVP + TS+TVSSTP Sbjct: 145 FSAGVPKFTSDTSSTVSSTP 164 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -3 Query: 121 TYRSHCLLPTRQLLRRTS*ISEI 53 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces pombe|chr 3|||Manual Length = 227 Score = 25.4 bits (53), Expect = 6.8 Identities = 17/64 (26%), Positives = 30/64 (46%) Frame = -3 Query: 571 FVNGHGVEDTVAEVQHDTGGTPDAYSESTAWMATYMAGVLNVSNMIWVIFSLLALGLRGA 392 FV+ ++T+ + + +PDAYS ++ + + I+V SL +LGL Sbjct: 162 FVSFTAADNTLPTITTPSSSSPDAYSSGSSRRTSNVLRQAYKKLGIFVERSLTSLGLSDR 221 Query: 391 SVSR 380 S R Sbjct: 222 SSER 225 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/39 (33%), Positives = 24/39 (61%) Frame = +2 Query: 395 SPQPQGQQREDDPDHVRNIQHARHVRRHPSRALAVRVRR 511 +PQ + E++ D++R++ +RH RR P +A + RR Sbjct: 1428 TPQSRSFTNENN-DNLRSV--SRHTRREPQQAQNLNARR 1463 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,955,527 Number of Sequences: 5004 Number of extensions: 66676 Number of successful extensions: 210 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 186 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 206 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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