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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-2065
         (600 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             134   5e-32
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)                57   1e-08
SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.002
SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.71 
SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.8  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  134 bits (324), Expect = 5e-32
 Identities = 61/92 (66%), Positives = 71/92 (77%), Gaps = 1/92 (1%)
 Frame = +3

Query: 72   VFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 251
            V F EKF D SWE  WV S   G + GKFK TAGKF+ D E DKG++TSEDA+FY +S K
Sbjct: 756  VHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGISAK 815

Query: 252  F-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLK 344
            F KPF+NEGK LV+QF+VKHEQ+IDCGGGY K
Sbjct: 816  FEKPFTNEGKTLVIQFSVKHEQNIDCGGGYAK 847



 Score = 37.9 bits (84), Expect = 0.006
 Identities = 19/40 (47%), Positives = 25/40 (62%)
 Frame = +3

Query: 435 KKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNT 554
           K + + FS K + ++       KDD  THLYTLIV+PDNT
Sbjct: 825 KTLVIQFSVKHEQNIDCGGGYAKDDEMTHLYTLIVRPDNT 864



 Score = 27.9 bits (59), Expect = 6.6
 Identities = 12/15 (80%), Positives = 13/15 (86%)
 Frame = +1

Query: 556 YEVLIDNXKVESGDL 600
           YEV IDN KVESG+L
Sbjct: 865 YEVKIDNEKVESGEL 879


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score = 56.8 bits (131), Expect = 1e-08
 Identities = 34/77 (44%), Positives = 44/77 (57%), Gaps = 10/77 (12%)
 Frame = +3

Query: 354 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKKDIRCK--------- 503
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN  I++    K         
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGE-DRKLHFIFRHKNPKNGTIEEKHAKKPTGNYNSVF 86

Query: 504 DDVYTHLYTLIVKPDNT 554
           D   THL+TL+V+PDNT
Sbjct: 87  DGKKTHLFTLVVRPDNT 103


>SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 539

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 3/98 (3%)
 Frame = -3

Query: 331 PPQSMSCSCLTVNWTTKGLPSLLNG---LNLRERA*NLASSEVFKPLSSSGSLKNFPAVN 161
           P +  SC CL V W T GLP L++G   L    R   L+   +  P   S SL +   V 
Sbjct: 429 PSEQNSCQCLFVGWQTVGLPQLIHGTVALISFRRKRTLSGGSL--PRGRSRSLSS-DRVG 485

Query: 160 LNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNA 47
              P++  G +    +    S+G+  S+ +SQ   D A
Sbjct: 486 TRLPSARKGSASKIGIPRTSSTGSLGSRRSSQSSTDGA 523


>SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3669

 Score = 31.1 bits (67), Expect = 0.71
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = -3

Query: 292  WTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSGSLKNF 173
            +TT+GLPS ++ +NL E   NL S    KP   S  + ++
Sbjct: 959  YTTQGLPSKISSVNLTEALSNLISISWSKPSDGSSLITDY 998


>SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 125

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = -2

Query: 134 VLTVHPIAFPRIIRKLLLKEYI 69
           +LT H +  P++IRK+ LK+Y+
Sbjct: 73  LLTAHLVVAPKLIRKMPLKDYV 94


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,555,047
Number of Sequences: 59808
Number of extensions: 451873
Number of successful extensions: 1082
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1017
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1077
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1451595000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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