Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-2065
(600 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 194 5e-50
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 193 9e-50
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 179 1e-45
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 109 1e-24
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 87 1e-17
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 77 9e-15
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 0.58
At5g56820.1 68418.m07090 F-box family protein contains F-box dom... 31 0.77
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.0
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 30 1.3
At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kina... 29 2.4
At3g49250.1 68416.m05382 expressed protein 29 3.1
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 3.1
At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family... 27 7.2
At2g37070.1 68415.m04549 expressed protein 27 7.2
At5g41320.1 68418.m05022 expressed protein 27 9.5
At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421... 27 9.5
At2g42290.1 68415.m05235 leucine-rich repeat family protein cont... 27 9.5
At1g74670.1 68414.m08647 gibberellin-responsive protein, putativ... 27 9.5
At1g31910.1 68414.m03921 GHMP kinase family protein contains TIG... 27 9.5
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 194 bits (472), Expect = 5e-50
Identities = 91/163 (55%), Positives = 116/163 (71%), Gaps = 2/163 (1%)
Frame = +3
Query: 72 VFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 245
V FEE+F DD WE+ WV SE + G++K TAG + D +DKG++TSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAIS 81
Query: 246 RKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICG 425
+F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPDICG
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICG 141
Query: 426 PGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNT 554
TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD T
Sbjct: 142 YSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDAT 184
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 193 bits (470), Expect = 9e-50
Identities = 88/167 (52%), Positives = 118/167 (70%), Gaps = 2/167 (1%)
Frame = +3
Query: 60 INCDVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 233
++ +V FEEKF +D WE WV S+ + G++K TAG + D +DKG++TSED RF
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Query: 234 YALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGP 413
YA+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGP
Sbjct: 78 YAISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGP 137
Query: 414 DICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNT 554
DICG TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD T
Sbjct: 138 DICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDAT 184
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 179 bits (435), Expect = 1e-45
Identities = 83/162 (51%), Positives = 113/162 (69%), Gaps = 2/162 (1%)
Frame = +3
Query: 69 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 242
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 243 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDIC 422
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPDIC
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDIC 146
Query: 423 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPD 548
G TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD
Sbjct: 147 GTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPD 188
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 109 bits (262), Expect = 1e-24
Identities = 53/114 (46%), Positives = 75/114 (65%), Gaps = 2/114 (1%)
Frame = +3
Query: 69 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 242
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 243 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIM 404
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 86.6 bits (205), Expect = 1e-17
Identities = 54/162 (33%), Positives = 85/162 (52%), Gaps = 10/162 (6%)
Frame = +3
Query: 96 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF-KPFSNE 272
D+ ++ W+ S++ E G +K S+ +D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 273 GKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGTKKV 443
+V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG GT KV
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG-GTNKV 149
Query: 444 HVIFSYKGK------NHLIKKDIRCKDDVYTHLYTLIVKPDN 551
H I +K H +K D +H+YT I+KPDN
Sbjct: 150 HFILKHKNPKSGEYVEHHLKFPPSVPYDKLSHVYTAILKPDN 191
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 77.0 bits (181), Expect = 9e-15
Identities = 53/165 (32%), Positives = 84/165 (50%), Gaps = 13/165 (7%)
Frame = +3
Query: 96 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF---KPFS 266
D+ ++ WV SE + G +K S+ DD GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEK----SEGHDDYGLLVSEKAKKYGIVKELDVDEPLN 90
Query: 267 -NEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGT 434
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-AT 148
Query: 435 KKVHVIFSYKGK------NHLIKKDIRCKDDVYTHLYTLIVKPDN 551
KVH I +K H +K D+ +H+YT ++K DN
Sbjct: 149 NKVHFILKHKNPKSGEFVEHHLKFPPSVPFDMLSHVYTAVLKSDN 193
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 0.58
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +3
Query: 288 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 401
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At5g56820.1 68418.m07090 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 30.7 bits (66), Expect = 0.77
Identities = 27/94 (28%), Positives = 49/94 (52%), Gaps = 6/94 (6%)
Frame = -3
Query: 265 LNG---LNLRERA*NLASSEVF-KPLSSSGSLKNFPAVNLNFPNSFPGC-SLYTQLLSHE 101
LNG L+L + + NL + ++ KPL S +K+ P +++ PNS P C + + + L +
Sbjct: 305 LNGDRILSLLKHSPNLQTLKLNEKPLRS---IKDQPNISVRKPNSVPECLTFHLETLEWQ 361
Query: 100 S-SGNFSSKNTSQFIEDNASKLTTTSTTAFIFDS 2
+G K + +I NA +L T + + + S
Sbjct: 362 GYAGRPEDKEIAVYILGNALRLNTATISRYFSSS 395
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.0
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +3
Query: 288 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 398
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.9 bits (64), Expect = 1.3
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -3
Query: 376 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNL 248
+ F S L K +P + MS V+W KG+ S L+GL L
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGLYL 189
>At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 769
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 115 LLSHESSGNFSSKNTSQFIEDNASKLTTTS 26
LLSH S+ N TS+ + + S+LTTTS
Sbjct: 218 LLSHNSTINIDDLRTSKAVSRSLSELTTTS 247
>At3g49250.1 68416.m05382 expressed protein
Length = 420
Score = 28.7 bits (61), Expect = 3.1
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 594 ARFNFXIVNEDFIRCCQVSQSECTN 520
A FNF E+F+RC ++QS CTN
Sbjct: 391 AVFNFGKKKEEFVRC--LAQSSCTN 413
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 3.1
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -3
Query: 382 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 281
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
>At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family
protein
Length = 466
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 372 DMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKK-DIRCKDDVYTHLYTL 533
D+ + + P+I G G K V +++ + K +KK ++ KD T LY L
Sbjct: 152 DIRATADFRQVGSPNIKGLGKKAVSTVYNGEDKPGFLKKLSLKFKDPENTTLYIL 206
>At2g37070.1 68415.m04549 expressed protein
Length = 420
Score = 27.5 bits (58), Expect = 7.2
Identities = 33/120 (27%), Positives = 57/120 (47%), Gaps = 5/120 (4%)
Frame = -3
Query: 385 SPCMSFCSSLQSKTLRYPPPQSMSCS----CLTVNWTTKGLPSLLNGLNLRERA*NLAS- 221
+P + F S+L+S S SCS CL+V+ T PS+ + ++++ +AS
Sbjct: 164 TPVVPFKSALRSSVASKNELTS-SCSSIESCLSVSSTASNKPSIHSVKQKKDQSLRIASH 222
Query: 220 SEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASK 41
S +P SS+GS +N L P G + + SS ++SS++ F + +K
Sbjct: 223 SLANRPKSSAGS-RNID--QLKVPPVSAGRTYKFNVSRLSSSVDWSSESPRAFTPNKMAK 279
>At5g41320.1 68418.m05022 expressed protein
Length = 515
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -3
Query: 220 SEVFKPLSSSGSLKNFPAVNLNFPNSFPG 134
S +F LSSS L++FP + FP+S G
Sbjct: 366 SSLFPKLSSSSLLQSFPVPSSLFPSSSQG 394
>At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421:
Protein of unknown function (DUF751)
Length = 185
Score = 27.1 bits (57), Expect = 9.5
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
Frame = +1
Query: 220 KMRGSMLSP---VSSNRSVMRVNPW*SSSLSNMNKTLTVEADTSRSLTANWSRRTCTER 387
KMR S L P +S+RS+ + SSS+ N L R LT SR + ER
Sbjct: 8 KMRLSCLQPHLHFTSSRSIAAALAFPSSSIRNRTSCLVRCGSARRRLTTKSSRNSKLER 66
>At2g42290.1 68415.m05235 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 646
Score = 27.1 bits (57), Expect = 9.5
Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 3/29 (10%)
Frame = -3
Query: 418 MSGPNIISYGVSP---CMSFCSSLQSKTL 341
M P+I+S VS CMSFCSSL S L
Sbjct: 1 MKLPSILSLVVSSIFLCMSFCSSLNSDGL 29
>At1g74670.1 68414.m08647 gibberellin-responsive protein, putative
similar to SP|P46690 Gibberellin-regulated protein 4
precursor {Arabidopsis thaliana} GASA4; contains Pfam
profile PF02704: Gibberellin regulated protein
Length = 101
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/37 (37%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -3
Query: 382 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 281
PCM FC +K L PP C C NW T+
Sbjct: 59 PCMFFCQKCCAKCLCVPPGTYGNKQVCPCYN-NWKTQ 94
>At1g31910.1 68414.m03921 GHMP kinase family protein contains
TIGRFAM profile TIGR01219: phosphomevalonate kinase;
contains Pfam PF00288: GHMP kinases putative ATP-binding
protein domain; similar to Phosphomevalonate kinase (EC
2.7.4.2) (Swiss-Prot:P24521) [Saccharomyces cerevisiae]
Length = 505
Score = 27.1 bits (57), Expect = 9.5
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +3
Query: 150 GKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKFKPFSNEGKP 281
GK +T G + + + GL S +ARFYA+ KP + E KP
Sbjct: 9 GKVLMTGGYLVLE-KPNAGLVLSTNARFYAI---VKPINEEVKP 48
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,672,419
Number of Sequences: 28952
Number of extensions: 306155
Number of successful extensions: 840
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 808
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 831
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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