Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-2046
(600 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 93 2e-19
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 88 5e-18
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 86 1e-17
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 83 1e-16
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 81 6e-16
At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 33 0.19
At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 29 2.4
At5g51630.2 68418.m06402 disease resistance protein (TIR-NBS-LRR... 28 4.1
At5g51630.1 68418.m06401 disease resistance protein (TIR-NBS-LRR... 28 4.1
At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 4.1
At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 28 5.4
At1g23550.1 68414.m02962 expressed protein 27 7.2
At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma m... 27 9.5
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 92.7 bits (220), Expect = 2e-19
Identities = 47/111 (42%), Positives = 67/111 (60%), Gaps = 2/111 (1%)
Frame = +3
Query: 111 VTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLLTSGGVTIAGTRYIYLSGTD 287
+T AAI GHDG+VWA+S F + E+ I+ F+ L G+ +AG +Y+ + G
Sbjct: 23 LTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEPGHLAPTGMFLAGLKYMVIQGEP 82
Query: 288 H-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 437
+ +IR K G G+ KT Q++V LYEEP+ P Q VVE+LG+YLI G
Sbjct: 83 NAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNMVVERLGDYLIEQG 133
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 87.8 bits (208), Expect = 5e-18
Identities = 45/111 (40%), Positives = 65/111 (58%), Gaps = 2/111 (1%)
Frame = +3
Query: 111 VTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLLTSGGVTIAGTRYIYLSGTD 287
+T AAI GHDG+VWA+S F + E + I+ F+ L G+ +AG +Y+ + G
Sbjct: 23 LTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEPGHLAPTGLFMAGAKYMVIQGEP 82
Query: 288 H-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 437
+IR K G G+ KT Q+ V +YEEP+ P Q VVE+LG+YL+ G
Sbjct: 83 GAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNMVVERLGDYLLEQG 133
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 86.2 bits (204), Expect = 1e-17
Identities = 46/116 (39%), Positives = 65/116 (56%), Gaps = 2/116 (1%)
Frame = +3
Query: 96 MASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLLTSGGVTIAGTRYIY 272
+A +T AAI G DG+VWA+S F ++ +E+ I F L G+ + G +Y+
Sbjct: 52 VAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTLAPTGLFLGGNKYMV 111
Query: 273 LSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 437
+ G + +IR K G GV KT A+V +Y+EP+ P Q VVE LGEYLI G
Sbjct: 112 IQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVENLGEYLIESG 167
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 83.4 bits (197), Expect = 1e-16
Identities = 46/111 (41%), Positives = 64/111 (57%), Gaps = 2/111 (1%)
Frame = +3
Query: 111 VTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLLTSGGVTIAGTRYIYLSG-T 284
+T AAI G DG+VWA+S F ++ E+A I FE L G+ + G +Y+ + G
Sbjct: 20 LTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHLAPTGLFLGGEKYMVVQGEA 79
Query: 285 DHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 437
+IR K G GV KT QA+V +Y+EP+ Q VVE+LG+YLI G
Sbjct: 80 GAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVERLGDYLIESG 130
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 81.0 bits (191), Expect = 6e-16
Identities = 44/108 (40%), Positives = 61/108 (56%), Gaps = 2/108 (1%)
Frame = +3
Query: 111 VTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLLTSGGVTIAGTRYIYLSGTD 287
+T AAI G DG+VWA+S F ++ E+ I FE L G+ + G +Y+ + G
Sbjct: 20 LTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFLAPTGLFLGGEKYMVIQGEQ 79
Query: 288 H-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLI 428
+IR K G GV KT QA+V Y+EP+ Q VVE+LG+YLI
Sbjct: 80 GAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVERLGDYLI 127
>At1g62420.1 68414.m07042 expressed protein contains Pfam profile
PF04720: Protein of unknown function (DUF506)
Length = 465
Score = 32.7 bits (71), Expect = 0.19
Identities = 16/63 (25%), Positives = 32/63 (50%)
Frame = +2
Query: 221 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 400
I +E RR +H+P W R + + W G ++ T++ ++ + T+ T+A + C
Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255
Query: 401 RGE 409
+ E
Sbjct: 256 KEE 258
>At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar
to sodium/calcium exchanger protein [Mus musculus]
gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 559
Score = 29.1 bits (62), Expect = 2.4
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -3
Query: 295 MIWSVPLR*MYRVPAIVTPPLVSSDSFSKPATI 197
+IW++ L +P I+T P+VS D +SKP +
Sbjct: 307 VIWAITLP--LNLPRILTIPVVSEDKWSKPLAV 337
>At5g51630.2 68418.m06402 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1175
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 2/48 (4%)
Frame = +3
Query: 69 GKIMSTKQLMASRCVTKAAIAGHDGNVWAKSEGF--EISKDEVAKIVA 206
GK KQ A+IAGHD W I+KD + K++A
Sbjct: 64 GKTEDVKQQWIEALEEVASIAGHDSKNWPNEANMIEHIAKDVLNKLIA 111
>At5g51630.1 68418.m06401 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1229
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 2/48 (4%)
Frame = +3
Query: 69 GKIMSTKQLMASRCVTKAAIAGHDGNVWAKSEGF--EISKDEVAKIVA 206
GK KQ A+IAGHD W I+KD + K++A
Sbjct: 128 GKTEDVKQQWIEALEEVASIAGHDSKNWPNEANMIEHIAKDVLNKLIA 175
>At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA7)
identical to SP|O64806 Potential calcium-transporting
ATPase 7, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong
similarity to SP|O81108 Calcium-transporting ATPase 2,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
2) {Arabidopsis thaliana}
Length = 1015
Score = 28.3 bits (60), Expect = 4.1
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGV 245
K+ GF+I DE+ IV G + + L GGV
Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119
>At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma
membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical
to SP|O22218 Calcium-transporting ATPase 4, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4)
{Arabidopsis thaliana}
Length = 1030
Score = 27.9 bits (59), Expect = 5.4
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGV 245
K GF I DE+A +V + +SL GGV
Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGV 118
>At1g23550.1 68414.m02962 expressed protein
Length = 323
Score = 27.5 bits (58), Expect = 7.2
Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
Frame = +3
Query: 75 IMSTKQLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVA-GFENESL 227
I + + +A R T A H G+ K + S+DE+ +I++ GF N +
Sbjct: 87 ITTRAKFLAFRIFTDAVARKHGGDANVKYGWYAGSRDEIQRIISYGFSNRDV 138
>At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma
membrane-type / Ca(2+)-ATPase isoform 2 (ACA2) identical
to SP|O81108 Calcium-transporting ATPase 2, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2)
{Arabidopsis thaliana}
Length = 1014
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGV 245
K+ GFEI DE+ IV + + L GGV
Sbjct: 90 KAAGFEICADELGSIVESHDVKKLKFHGGV 119
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,069,735
Number of Sequences: 28952
Number of extensions: 267467
Number of successful extensions: 588
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 563
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 583
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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