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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1322
         (505 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_7742| Best HMM Match : HEAT (HMM E-Value=9e-23)                    107   6e-24
SB_34262| Best HMM Match : efhand (HMM E-Value=6.3e-26)                29   2.2  
SB_55427| Best HMM Match : E-MAP-115 (HMM E-Value=0.077)               28   3.8  
SB_44703| Best HMM Match : Extensin_2 (HMM E-Value=0.077)              27   6.6  
SB_16391| Best HMM Match : AMP-binding (HMM E-Value=1.5e-10)           27   8.8  

>SB_7742| Best HMM Match : HEAT (HMM E-Value=9e-23)
          Length = 940

 Score =  107 bits (256), Expect(2) = 6e-24
 Identities = 52/130 (40%), Positives = 78/130 (60%)
 Frame = +2

Query: 116 DVRQTAAVLLRRLFSAXXXXXXXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCDVVSEL 295
           +VRQ AAVLLRR+F+A            Q  +++E LL  +  +    +R+K+CD VSEL
Sbjct: 27  EVRQMAAVLLRRIFTATVDFLKKIDENTQN-LMKESLLKGIHEEQDSNVRKKICDAVSEL 85

Query: 296 ARNHIDDDGNNQWPEFLQFMFTCASAQDPNIKEAGIRMFTSVPGVFGNRHTENLDVIKGM 475
           +++ +DDDG N W E L+F+F C ++    +KE+ + +F S PGVFGN+    L+VIK M
Sbjct: 86  SKSFLDDDGYNHWQELLKFLFECCNSPRAELKESALNIFCSFPGVFGNQQDHYLNVIKQM 145

Query: 476 LISALQPNES 505
           L   +    S
Sbjct: 146 LWQCINDQTS 155



 Score = 21.0 bits (42), Expect(2) = 6e-24
 Identities = 8/12 (66%), Positives = 10/12 (83%)
 Frame = +2

Query: 2  LSTDNDIRSQAE 37
          +S DND R+QAE
Sbjct: 16 MSPDNDTRNQAE 27


>SB_34262| Best HMM Match : efhand (HMM E-Value=6.3e-26)
          Length = 354

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
 Frame = +2

Query: 269 KVCDVVSELARNHIDDDGNN--QWPEFLQFMFTCASAQDPN 385
           ++ D+++++A   +D DGN    +PEFLQ M       DP+
Sbjct: 249 ELMDMMNQIAFLFVDSDGNGAIDFPEFLQLMTKNLQDADPD 289


>SB_55427| Best HMM Match : E-MAP-115 (HMM E-Value=0.077)
          Length = 599

 Score = 28.3 bits (60), Expect = 3.8
 Identities = 16/39 (41%), Positives = 19/39 (48%), Gaps = 1/39 (2%)
 Frame = -2

Query: 447 VWR-FPNTPGTDVNIRIPASLMFGSCALAQVNMNCRNSG 334
           VWR FP TPG D N  +P     G  A+ Q   + R  G
Sbjct: 543 VWRWFPRTPGLDRNCPLPLPGQEGRHAVLQQERHNRYQG 581


>SB_44703| Best HMM Match : Extensin_2 (HMM E-Value=0.077)
          Length = 757

 Score = 27.5 bits (58), Expect = 6.6
 Identities = 13/50 (26%), Positives = 23/50 (46%)
 Frame = +2

Query: 302 NHIDDDGNNQWPEFLQFMFTCASAQDPNIKEAGIRMFTSVPGVFGNRHTE 451
           + +D     Q+P  L + + C    D  + E G  +  ++PG  G RH +
Sbjct: 682 SQLDPGHKLQFPVILTYKYAC----DSQVMEVGATIPATIPGGCGGRHMQ 727


>SB_16391| Best HMM Match : AMP-binding (HMM E-Value=1.5e-10)
          Length = 418

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 15/44 (34%), Positives = 20/44 (45%)
 Frame = +1

Query: 64  NKSSAPSEFNSKCRHCRRCASNGGRAVTKII*CRIL*VLPEIAI 195
           N  S   + N K  H       GG+  TK I  R+L  LP+I +
Sbjct: 153 NLDSFKVDRNKKISHMANYVILGGQLYTKAITARLLDALPDITV 196


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,529,966
Number of Sequences: 59808
Number of extensions: 330699
Number of successful extensions: 968
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1099461690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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