BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-1322 (505 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g19820.1 68418.m02355 PBS lyase HEAT-like repeat-containing p... 75 2e-14 At4g27640.1 68417.m03973 importin beta-2 subunit family protein ... 65 2e-11 At2g47230.1 68415.m05898 agenet domain-containing protein contai... 35 0.036 At5g16000.1 68418.m01871 leucine-rich repeat family protein / pr... 30 0.77 At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing ... 27 5.4 At3g55830.1 68416.m06203 glycosyltransferase family protein 47 s... 27 9.5 At3g27250.1 68416.m03406 expressed protein 27 9.5 At2g43980.1 68415.m05469 inositol 1,3,4-trisphosphate 5/6-kinase... 27 9.5 >At5g19820.1 68418.m02355 PBS lyase HEAT-like repeat-containing protein contains Pfam profile: PF03130 PBS lyase HEAT-like repeat Length = 1116 Score = 75.4 bits (177), Expect = 2e-14 Identities = 46/171 (26%), Positives = 84/171 (49%), Gaps = 3/171 (1%) Frame = +2 Query: 2 LSTDNDIRSQAEDAYN---NIPTETKVVHLVNSIQNADIAEDVRQTAAVLLRRLFSAXXX 172 +S+ N+ RS AE +N +T + L + +Q + E R AAVLLR+L + Sbjct: 32 MSSSNEQRSSAESLFNLAKQSNPDTLSLKLAHLLQLSPHPEG-RAMAAVLLRKLLTRDDA 90 Query: 173 XXXXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCDVVSELARNHIDDDGNNQWPEFLQF 352 Q+ L+ +L +Q + ++ + +K+CD VSELA + ++G WPE L F Sbjct: 91 YLWPRLSLSTQSSLKSSMLYCIQHEEAKSISKKICDTVSELASGILPENG---WPELLPF 147 Query: 353 MFTCASAQDPNIKEAGIRMFTSVPGVFGNRHTENLDVIKGMLISALQPNES 505 +F C ++ P ++E+ + + G T ++ + G+ + L N + Sbjct: 148 VFQCVTSVTPKLQESAFLILAQLSQYVGETLTPHIKELHGVFLQCLSSNSA 198 >At4g27640.1 68417.m03973 importin beta-2 subunit family protein low similarity to importin 4 GI:18700635 from [Homo sapiens] Length = 1048 Score = 65.3 bits (152), Expect = 2e-11 Identities = 47/169 (27%), Positives = 84/169 (49%), Gaps = 1/169 (0%) Frame = +2 Query: 2 LSTDNDIRSQAEDAYNNIPTETKVVH-LVNSIQNADIAEDVRQTAAVLLRRLFSAXXXXX 178 L DND R QAED + + +VV LV ++ A +VRQ AAVLLR+ + Sbjct: 13 LMPDNDARRQAEDQIKRLAKDPQVVPALVQHLRTAK-TPNVRQLAAVLLRKRITGHWAKL 71 Query: 179 XXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCDVVSELARNHIDDDGNNQWPEFLQFMF 358 +Q V ++ L+ ++ ++ S +RR +VVS +A+ + +WP+ L F+F Sbjct: 72 SPQL---KQHV-KQSLIESITVENSPPVRRASANVVSVVAKYAVPA---GEWPDLLTFLF 124 Query: 359 TCASAQDPNIKEAGIRMFTSVPGVFGNRHTENLDVIKGMLISALQPNES 505 C+ + + +E + +F+S+ GN ++ +L+ +Q S Sbjct: 125 QCSQSAQEDHREVALILFSSLTETIGNTFRPYFADLQALLLKCMQDESS 173 >At2g47230.1 68415.m05898 agenet domain-containing protein contains Pfam PF05641: Agenet domain Length = 701 Score = 34.7 bits (76), Expect = 0.036 Identities = 17/41 (41%), Positives = 23/41 (56%) Frame = +2 Query: 14 NDIRSQAEDAYNNIPTETKVVHLVNSIQNADIAEDVRQTAA 136 ND+ Q A+ N+PTET + H + NA IA DV + A Sbjct: 467 NDVDDQPLAAWINLPTETSIDHSPIVVNNAAIATDVEERQA 507 >At5g16000.1 68418.m01871 leucine-rich repeat family protein / protein kinase family protein contains Pfam domains PF00560: Leucine Rich Repeat and PF00069: Protein kinase domain Length = 638 Score = 30.3 bits (65), Expect = 0.77 Identities = 8/26 (30%), Positives = 18/26 (69%) Frame = -1 Query: 460 IQVFSVAISKYTWYRRKHSNTSFFDV 383 + + +A+ + W+R++H+ +FFDV Sbjct: 258 VSLIFIAVGLFLWWRQRHNQNTFFDV 283 >At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing protein prolamin box binding factor, Zea mays, PID:g2393775 Length = 194 Score = 27.5 bits (58), Expect = 5.4 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +1 Query: 58 NRNKSSAPSEFNSKCRHCRRCASNGG 135 N NK S P KC++C RC ++GG Sbjct: 37 NNNKKSQP---RYKCKNCCRCWTHGG 59 >At3g55830.1 68416.m06203 glycosyltransferase family protein 47 similar to exostose-related protein 2, Homo sapiens, PIR:JC5935 [SP|Q93063], EXTL2, Mus musculus [GI:10443633] Length = 334 Score = 26.6 bits (56), Expect = 9.5 Identities = 16/56 (28%), Positives = 23/56 (41%) Frame = -2 Query: 414 VNIRIPASLMFGSCALAQVNMNCRNSGHWLLPSSSI*FLASSETTSQTFLRKYWER 247 V R L+F A V + CR+S W+ S ++ S T L W+R Sbjct: 28 VTARSTKFLLFCCIAFVLVTIVCRSSRPWVNSSIAVADRISGSRKGYTLLMNTWKR 83 >At3g27250.1 68416.m03406 expressed protein Length = 282 Score = 26.6 bits (56), Expect = 9.5 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = -1 Query: 499 VWLQSRY*HAFYHI--QVFSVAIS-KYTWYRRKHSNTSFFDVWILRTG 365 VWL + A H Q F+ + +YTW K++ T DVW + G Sbjct: 219 VWLVAEEATAAMHCNYQAFTCRVRMQYTWKETKYTKTVPCDVWKMEFG 266 >At2g43980.1 68415.m05469 inositol 1,3,4-trisphosphate 5/6-kinase family protein contains Pfam profile PF05770: Inositol 1, 3, 4-trisphosphate 5/6-kinase; contains Prosite PS00591: Glycosyl hydrolases family 10 active site; similar to inositol 1,3,4-trisphosphate 5/6-kinase (GI:1322038) [Homo sapiens] Length = 488 Score = 26.6 bits (56), Expect = 9.5 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%) Frame = -1 Query: 427 TWYRRKHSNTSF-FDVWILRTGTGKHEL*ELRPL 329 TW R+K T F FDV +++ GTG H + +L L Sbjct: 425 TWLRKKLDLTIFGFDV-VIQEGTGDHVIVDLNYL 457 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,397,340 Number of Sequences: 28952 Number of extensions: 231460 Number of successful extensions: 610 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 599 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 607 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 898188928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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