Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1294
(629 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30450.3 68414.m03722 cation-chloride cotransporter, putative... 139 1e-33
At1g30450.2 68414.m03721 cation-chloride cotransporter, putative... 139 1e-33
At1g30450.1 68414.m03720 cation-chloride cotransporter, putative... 139 1e-33
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 31 0.63
At1g04390.1 68414.m00429 expressed protein 31 0.83
At4g19590.1 68417.m02879 DNAJ heat shock N-terminal domain-conta... 29 1.9
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 1.9
At1g33265.1 68414.m04113 expressed protein contains Pfam profile... 29 1.9
At1g75220.1 68414.m08738 integral membrane protein, putative str... 29 2.5
At1g55430.1 68414.m06340 DC1 domain-containing protein contains ... 29 2.5
At4g37370.1 68417.m05292 cytochrome P450, putative similar to Cy... 29 3.4
At2g16920.1 68415.m01949 ubiquitin-conjugating enzyme family pro... 28 4.4
At1g26140.1 68414.m03191 hypothetical protein 28 5.9
At1g07350.1 68414.m00783 transformer serine/arginine-rich ribonu... 28 5.9
At1g04280.1 68414.m00419 expressed protein 28 5.9
At4g34150.1 68417.m04846 C2 domain-containing protein similar to... 27 7.8
At3g42640.1 68416.m04431 ATPase, plasma membrane-type, putative ... 27 7.8
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 27 7.8
>At1g30450.3 68414.m03722 cation-chloride cotransporter, putative
similar to cation-chloride co-transporter GB:AAC49874
GI:2582381 from [Nicotiana tabacum], Cation-Chloride
Cotransporter (CCC) Family Member, PMID:11500563
Length = 975
Score = 139 bits (337), Expect = 1e-33
Identities = 62/120 (51%), Positives = 86/120 (71%)
Frame = +3
Query: 192 RMGTLIGVYLPCIQNIFGVILFIRLTWVVGTAGAIQGFLIVLTCCCTTMLTAISMSAIAT 371
+MGT++GV++PC+QNI G+I +IR TW+VG AG QG ++V C T LT IS+SAIAT
Sbjct: 131 KMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIAT 190
Query: 372 NGVVPAGGSYFMIGRSLGPECGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWISIFR 551
NG + GG Y++IGR+LGPE G ++G+ F+ G +A A+Y++GAVE L P IFR
Sbjct: 191 NGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGALYVLGAVETFLKAF-PAAGIFR 249
>At1g30450.2 68414.m03721 cation-chloride cotransporter, putative
similar to cation-chloride co-transporter GB:AAC49874
GI:2582381 from [Nicotiana tabacum], Cation-Chloride
Cotransporter (CCC) Family Member, PMID:11500563
Length = 975
Score = 139 bits (337), Expect = 1e-33
Identities = 62/120 (51%), Positives = 86/120 (71%)
Frame = +3
Query: 192 RMGTLIGVYLPCIQNIFGVILFIRLTWVVGTAGAIQGFLIVLTCCCTTMLTAISMSAIAT 371
+MGT++GV++PC+QNI G+I +IR TW+VG AG QG ++V C T LT IS+SAIAT
Sbjct: 131 KMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIAT 190
Query: 372 NGVVPAGGSYFMIGRSLGPECGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWISIFR 551
NG + GG Y++IGR+LGPE G ++G+ F+ G +A A+Y++GAVE L P IFR
Sbjct: 191 NGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGALYVLGAVETFLKAF-PAAGIFR 249
>At1g30450.1 68414.m03720 cation-chloride cotransporter, putative
similar to cation-chloride co-transporter GB:AAC49874
GI:2582381 from [Nicotiana tabacum], Cation-Chloride
Cotransporter (CCC) Family Member, PMID:11500563
Length = 975
Score = 139 bits (337), Expect = 1e-33
Identities = 62/120 (51%), Positives = 86/120 (71%)
Frame = +3
Query: 192 RMGTLIGVYLPCIQNIFGVILFIRLTWVVGTAGAIQGFLIVLTCCCTTMLTAISMSAIAT 371
+MGT++GV++PC+QNI G+I +IR TW+VG AG QG ++V C T LT IS+SAIAT
Sbjct: 131 KMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIAT 190
Query: 372 NGVVPAGGSYFMIGRSLGPECGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWISIFR 551
NG + GG Y++IGR+LGPE G ++G+ F+ G +A A+Y++GAVE L P IFR
Sbjct: 191 NGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGALYVLGAVETFLKAF-PAAGIFR 249
>At5g57320.1 68418.m07160 villin, putative similar to villin 2 (VLN2)
[Arabidopsis thaliana] GI:3415115, villin 3 (VLN3)
[Arabidopsis thaliana] GI:3415117; contains Pfam profiles
PF00626: Gelsolin repeat, PF02209: Villin headpiece
domain
Length = 962
Score = 31.1 bits (67), Expect = 0.63
Identities = 27/84 (32%), Positives = 37/84 (44%), Gaps = 5/84 (5%)
Frame = -2
Query: 475 RVVPV*NSMPTAPPHSGPRDRPIMKYDPP----AGTTP-FVAMADIDIAVNIVVQQQVRT 311
R VP +S T P S PR R M + P G +P F A+A +NI Q
Sbjct: 752 RRVPAYSSRSTVPDKSQPRSRS-MTFSPDRARVRGRSPAFNALAANFEKLNIRNQSTPPP 810
Query: 310 MRNPCIAPAVPTTQVSRMNKITPK 239
M +P + P + ++KI PK
Sbjct: 811 MVSPMVRKLYPKSHAPDLSKIAPK 834
>At1g04390.1 68414.m00429 expressed protein
Length = 849
Score = 30.7 bits (66), Expect = 0.83
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 167 CPQASPTSPHGHAHRRVPALHPEYLR 244
C S TSPH H+HR + + EYLR
Sbjct: 671 CRMCSLTSPHAHSHRVILSSGCEYLR 696
>At4g19590.1 68417.m02879 DNAJ heat shock N-terminal
domain-containing protein protein YJL162c, Saccharomyces
cerevisiae, PIR2:S56945; contains Pfam PF00226: DnaJ
domain;
Length = 345
Score = 29.5 bits (63), Expect = 1.9
Identities = 14/41 (34%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +1
Query: 73 KRSRTDLEQPRSSAR*RTMRIQSPRHPP-QTQMPPSQPHQP 192
KRSR + P+ + + PP Q + PP+QP QP
Sbjct: 128 KRSRKQKQPPKKPPNQPKQQPNQQKQPPDQQKQPPNQPRQP 168
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 29.5 bits (63), Expect = 1.9
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +2
Query: 140 PHGIRRRPRCPQASPTSPHGHAHRRVPALHP 232
PH R +P C + P PH RR P+ P
Sbjct: 6 PHFSRAKPPCRLSDPAQPHKLCRRRRPSYPP 36
>At1g33265.1 68414.m04113 expressed protein contains Pfam profile
PF03647: Uncharacterised protein family (UPF0136);
supporting cDNA gi|23198247|gb|BT000332.1|
Length = 177
Score = 29.5 bits (63), Expect = 1.9
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 2/60 (3%)
Frame = +3
Query: 324 CCTTMLTAISMSAIATNGVVPAGGSYFMIGRSLGPECGGAVGMLF--YTGTTLAAAMYIV 497
CC L+ ++ AT GV+ GG F +S G+ G LF TG+ L A+ Y +
Sbjct: 51 CCKAELSELAPVVSATYGVLLLGGGLFAYSKS------GSKGSLFGGLTGSVLMASAYFL 104
>At1g75220.1 68414.m08738 integral membrane protein, putative strong
similarity to integral membrane protein GI:1209756 from
[Beta vulgaris]; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 487
Score = 29.1 bits (62), Expect = 2.5
Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 8/52 (15%)
Frame = +3
Query: 408 IGRSLGPECGGAVGMLFYTGTTLA--------AAMYIVGAVEIVLTYMAPWI 539
IG + + GG G+LFY+ T AA + VGA+++V T ++ W+
Sbjct: 288 IGLLVLQQLGGINGVLFYSSTIFESAGVTSSNAATFGVGAIQVVATAISTWL 339
>At1g55430.1 68414.m06340 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 657
Score = 29.1 bits (62), Expect = 2.5
Identities = 15/44 (34%), Positives = 18/44 (40%)
Frame = +2
Query: 140 PHGIRRRPRCPQASPTSPHGHAHRRVPALHPEYLRCDLIHPAHL 271
P+ R +P C + P PH RR P HP C HL
Sbjct: 12 PYLTRTKPPCRLSDPAQPHKLCRRRQPT-HPPLSICFTCKGKHL 54
>At4g37370.1 68417.m05292 cytochrome P450, putative similar to
Cytochrome P450 91A1 (SP:Q9FG65 )[Arabidopsis thaliana];
cytochrome P450, Glycyrrhiza echinata, AB001379
Length = 497
Score = 28.7 bits (61), Expect = 3.4
Identities = 17/57 (29%), Positives = 24/57 (42%)
Frame = -2
Query: 550 RKIDIHGAM*VSTISTAPTMYIAAARVVPV*NSMPTAPPHSGPRDRPIMKYDPPAGT 380
RKI + M S IS P + + + + + P PH D + YD P GT
Sbjct: 331 RKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGT 387
>At2g16920.1 68415.m01949 ubiquitin-conjugating enzyme family
protein low similarity to ubiquitin-conjugating
BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
contains Pfam profile PF00179: Ubiquitin-conjugating
enzyme
Length = 1102
Score = 28.3 bits (60), Expect = 4.4
Identities = 16/67 (23%), Positives = 30/67 (44%)
Frame = -1
Query: 323 AG*NNEESLYSASGADNPGEPDE*DHTEDILDARQVHADERAHAGWWGWLGGIWVCGGCR 144
AG ++ +S S D+ + D D ED+ + ++ + + G + CG
Sbjct: 58 AGDSDSDSDISDEEEDDDDDEDNDDDDEDVEEGKKASEENVVNGDGEKKADGNYKCGALE 117
Query: 143 GDCIRIV 123
GD IR++
Sbjct: 118 GDQIRVL 124
>At1g26140.1 68414.m03191 hypothetical protein
Length = 162
Score = 27.9 bits (59), Expect = 5.9
Identities = 9/36 (25%), Positives = 20/36 (55%)
Frame = +3
Query: 249 ILFIRLTWVVGTAGAIQGFLIVLTCCCTTMLTAISM 356
++F+ + + GA +VL+CCC ++ +S+
Sbjct: 37 VIFVHIRVMEEDCGAFAADCVVLSCCCQCLVLQVSV 72
>At1g07350.1 68414.m00783 transformer serine/arginine-rich
ribonucleoprotein, putative similar to GB:Y09506 from
[Nicotiana tabacum] (Plant Mol. Biol. 35 (3), 261-269
(1997))
Length = 382
Score = 27.9 bits (59), Expect = 5.9
Identities = 18/53 (33%), Positives = 22/53 (41%)
Frame = +2
Query: 134 YNPHGIRRRPRCPQASPTSPHGHAHRRVPALHPEYLRCDLIHPAHLGCRHRWR 292
Y+P RRR P SP+ + RR + P Y R D CR R R
Sbjct: 216 YSPFYRRRRFYSPSRSPSPDDRYNRRRDRSYSPYYRRRDRSRSYSRNCRARDR 268
>At1g04280.1 68414.m00419 expressed protein
Length = 480
Score = 27.9 bits (59), Expect = 5.9
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 146 GIRRRPRCPQASPTSPHGHAHRRVPALHPEYLRCDLIHPAHLGC 277
GIRR +A P ++H+R P+Y C+L+ A L C
Sbjct: 349 GIRRALMVKRAVRVKPQLNSHKRFANAFPKY--CELVDNARLYC 390
>At4g34150.1 68417.m04846 C2 domain-containing protein similar to
calcium-dependent protein kinase [Dunaliella
tertiolecta] GI:6644464; contains Pfam profile PF00168:
C2 domain
Length = 247
Score = 27.5 bits (58), Expect = 7.8
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = -2
Query: 445 TAPPHSGPRDRP-IMKYDPPAGTTP 374
TAPP+SGP P + +Y P+G P
Sbjct: 162 TAPPYSGPSLYPQVQQYPQPSGYPP 186
>At3g42640.1 68416.m04431 ATPase, plasma membrane-type, putative /
proton pump, putative strong similarity to P-type
H+-ATPase from [Lycopersicon esculentum] GI:1621440,
[Solanum tuberosum] GI:435001, SP|Q03194 {Nicotiana
plumbaginifolia}; contains InterPro accession IPR001757:
ATPase, E1-E2 type
Length = 948
Score = 27.5 bits (58), Expect = 7.8
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -1
Query: 185 WGWLGGIWV 159
WGW GGIWV
Sbjct: 820 WGWAGGIWV 828
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.5 bits (58), Expect = 7.8
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +2
Query: 140 PHGIRRRPRCPQASPTSPHGHAHRRVPALHPEYLRC 247
P+ R +P C + P PH RR P+ HP C
Sbjct: 6 PYFSRAKPPCRLSDPAQPHMLCRRRRPS-HPPLSIC 40
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,810,370
Number of Sequences: 28952
Number of extensions: 325948
Number of successful extensions: 1227
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1072
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1222
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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