Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1289
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 220 6e-58
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 220 6e-58
At3g01780.1 68416.m00118 expressed protein est hit, 32 0.32
At4g00560.1 68417.m00077 methionine adenosyltransferase regulato... 30 1.3
At4g31115.1 68417.m04416 expressed protein supported by full len... 30 1.7
At5g56390.1 68418.m07039 F-box family protein contains F-box dom... 29 2.2
At5g61500.1 68418.m07717 autophagy 3 (APG3) identical to autopha... 29 3.9
At3g01490.1 68416.m00073 protein kinase, putative similar to ATM... 29 3.9
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 28 5.2
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 28 5.2
At1g30420.1 68414.m03718 ATP-binding cassette transport protein,... 28 5.2
At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-th... 28 6.8
At4g21130.1 68417.m03055 transducin family protein / WD-40 repea... 27 9.0
At3g15030.2 68416.m01902 TCP family transcription factor, putati... 27 9.0
At3g15030.1 68416.m01901 TCP family transcription factor, putati... 27 9.0
>At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-coat
protein, putative / beta-COP, putative similar to
Coatomer beta subunit (Beta-coat protein) (Beta-COP) from
{Rattus norvegicus} SP|P23514, {Mus musculus} SP|Q9JIF7,
{Homo sapiens} SP|P53618; contains Pfam profile: PF01602
Adaptin N terminal region
Length = 948
Score = 220 bits (538), Expect = 6e-58
Identities = 103/170 (60%), Positives = 131/170 (77%)
Frame = +1
Query: 190 DRGKLCRVRQLTGCSDPVYAEAIVSVNQYDIVLDVLLVNQTDDTLQNCCVELATLGELRL 369
D KL R+ QLTG SDPVYAEA V+V+ YDI L+V ++N+T +TLQN C+ELAT+G+L+L
Sbjct: 710 DANKLNRILQLTGFSDPVYAEAYVTVHHYDIALEVTVINRTKETLQNLCLELATMGDLKL 769
Query: 370 VERAAEVVLAPRDFASLRAHVKVASTENGVIFGNVVYEVTGASMDRGVVVLNDIHIDIVD 549
VER LAP ++A++KV+STE GVIFGN+VYE T M+R VVVLNDIHIDI+D
Sbjct: 770 VERPQNYSLAPERSMQIKANIKVSSTETGVIFGNIVYE-TSNVMERNVVVLNDIHIDIMD 828
Query: 550 FIQPAHCSDADFRQMWAEFEWENKVSVNTNMTDLHEYLQHLLAFTNMKCL 699
+I PA CS+ FR MWAEFEWENKV+VNT + + E+L H++ TNMKCL
Sbjct: 829 YISPAVCSEVAFRTMWAEFEWENKVAVNTTIQNEREFLDHIIKSTNMKCL 878
>At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-coat
protein, putative / beta-COP, putative similar to
Coatomer beta subunit (Beta-coat protein) (Beta-COP) from
{Rattus norvegicus} SP|P23514, {Mus musculus} SP|Q9JIF7,
{Homo sapiens} SP|P53618; contains Pfam profile: PF01602
Adaptin N terminal region
Length = 971
Score = 220 bits (538), Expect = 6e-58
Identities = 103/170 (60%), Positives = 131/170 (77%)
Frame = +1
Query: 190 DRGKLCRVRQLTGCSDPVYAEAIVSVNQYDIVLDVLLVNQTDDTLQNCCVELATLGELRL 369
D KL R+ QLTG SDPVYAEA V+V+ YDI L+V ++N+T +TLQN C+ELAT+G+L+L
Sbjct: 733 DANKLNRILQLTGFSDPVYAEAYVTVHHYDIALEVTVINRTKETLQNLCLELATMGDLKL 792
Query: 370 VERAAEVVLAPRDFASLRAHVKVASTENGVIFGNVVYEVTGASMDRGVVVLNDIHIDIVD 549
VER LAP ++A++KV+STE GVIFGN+VYE T M+R VVVLNDIHIDI+D
Sbjct: 793 VERPQNYSLAPERSMQIKANIKVSSTETGVIFGNIVYE-TSNVMERNVVVLNDIHIDIMD 851
Query: 550 FIQPAHCSDADFRQMWAEFEWENKVSVNTNMTDLHEYLQHLLAFTNMKCL 699
+I PA CS+ FR MWAEFEWENKV+VNT + + E+L H++ TNMKCL
Sbjct: 852 YISPAVCSEVAFRTMWAEFEWENKVAVNTTIQNEREFLDHIIKSTNMKCL 901
>At3g01780.1 68416.m00118 expressed protein est hit,
Length = 1176
Score = 32.3 bits (70), Expect = 0.32
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +1
Query: 220 LTGCSDPVYAEA--IVSVNQYDIVLDVLLVNQTDDTLQNCCVELATLGELRLVERAAEVV 393
LTG SDP Y EA + N + L + ++N T+ L + + G L ++ + + V
Sbjct: 772 LTGSSDPCYIEAYHLADTNDGRVTLHLKIINLTELELNRVDIRVGLSGALYFMDGSPQAV 831
Query: 394 LAPRDFAS 417
R+ S
Sbjct: 832 RQLRNLVS 839
>At4g00560.1 68417.m00077 methionine adenosyltransferase regulatory
beta subunit-related contains weak similarity to
methionine adenosyltransferase regulatory beta subunit
(GI:6815285) [Homo sapiens]
Length = 327
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +1
Query: 469 NVVYEVTGASMDRGVVVLNDIHIDIVDFIQPAHCSDADFRQ 591
+++ V+ +S+DRGVV DI +DI I S F++
Sbjct: 271 SLIKHVSASSIDRGVVSPADISMDITKLIHTLELSPTSFKE 311
>At4g31115.1 68417.m04416 expressed protein supported by full length
cDNA gi:21436034 from [Arabidopsis thaliana]
Length = 250
Score = 29.9 bits (64), Expect = 1.7
Identities = 20/69 (28%), Positives = 31/69 (44%)
Frame = +1
Query: 139 ELALNKALQGRSGGEAIDRGKLCRVRQLTGCSDPVYAEAIVSVNQYDIVLDVLLVNQTDD 318
EL ++ L+ SG EA+ K + L SD Y + V + +LV +
Sbjct: 77 ELTFSEFLKHPSGMEAVINAKALQSYHLVDDSDDTYRCTLPKVQLMSFEVYPVLVLRVTP 136
Query: 319 TLQNCCVEL 345
T ++C VEL
Sbjct: 137 TQEDCTVEL 145
>At5g56390.1 68418.m07039 F-box family protein contains F-box domain
Pfam:PF00646
Length = 428
Score = 29.5 bits (63), Expect = 2.2
Identities = 25/80 (31%), Positives = 40/80 (50%), Gaps = 6/80 (7%)
Frame = +1
Query: 283 VLDVLLVNQ-TDDTLQNCCVELATLGELRLVERAAEVVLAPRDFA----SLRAHVKVAS- 444
VL+ L+V Q +DD + V + +L L L++RA + P DF SL H+K+
Sbjct: 182 VLEDLVVKQCSDDNVTILTVRVPSLKRLSLIQRAELEIDVPHDFVIDTPSLE-HLKIVDY 240
Query: 445 TENGVIFGNVVYEVTGASMD 504
T + + + + ASMD
Sbjct: 241 TYGSRVVKSTMNRIITASMD 260
>At5g61500.1 68418.m07717 autophagy 3 (APG3) identical to autophagy
3 [Arabidopsis thaliana] GI:19912141; contains Pfam
profiles PF03986: Autophagocytosis associated protein
N-terminal domain, PF03987: Autophagocytosis associated
protein C-terminal domain
Length = 313
Score = 28.7 bits (61), Expect = 3.9
Identities = 16/47 (34%), Positives = 27/47 (57%)
Frame = -2
Query: 567 VRRLDEVDNVNVDVIQHDHAAVHGRSSHFVYHVPEDDAVLRGGDLDV 427
+ DE DNV +++D A + +S++ V H P+DD +LR D+
Sbjct: 157 MEEFDEADNV----VENDPATL--QSTYLVAHEPDDDNILRTRTYDL 197
>At3g01490.1 68416.m00073 protein kinase, putative similar to ATMRK1
[Arabidopsis thaliana] gi|2351097|dbj|BAA22079
Length = 411
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/53 (26%), Positives = 28/53 (52%)
Frame = +1
Query: 457 VIFGNVVYEVTGASMDRGVVVLNDIHIDIVDFIQPAHCSDADFRQMWAEFEWE 615
+I +V+ T ++ RG+ D+ + ++D+ + H SDA+ + A F E
Sbjct: 108 LIIKSVIARGTFGTVHRGIYDGQDVAVKLLDWGEEGHRSDAEIASLRAAFTQE 160
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 24/83 (28%), Positives = 40/83 (48%), Gaps = 1/83 (1%)
Frame = +1
Query: 259 VSVNQYDIVLDVLLVNQTDDTLQNCCVELATLGELRLVERAAEVVLAPRDFASLRAHVKV 438
++V YD + +L ++ DD LQ V+ + L+ + + D A A++ +
Sbjct: 602 LAVGSYDNTVRILSLDP-DDCLQILSVQSVSSAPESLLFLEVQASIGGDDGADHPANLFL 660
Query: 439 AS-TENGVIFGNVVYEVTGASMD 504
S +NGV+F VV VTG D
Sbjct: 661 NSGLQNGVLFRTVVDMVTGQLSD 683
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 24/83 (28%), Positives = 40/83 (48%), Gaps = 1/83 (1%)
Frame = +1
Query: 259 VSVNQYDIVLDVLLVNQTDDTLQNCCVELATLGELRLVERAAEVVLAPRDFASLRAHVKV 438
++V YD + +L ++ DD LQ V+ + L+ + + D A A++ +
Sbjct: 602 LAVGSYDNTVRILSLDP-DDCLQILSVQSVSSAPESLLFLEVQASIGGDDGADHPANLFL 660
Query: 439 AS-TENGVIFGNVVYEVTGASMD 504
S +NGV+F VV VTG D
Sbjct: 661 NSGLQNGVLFRTVVDMVTGQLSD 683
>At1g30420.1 68414.m03718 ATP-binding cassette transport protein,
putative contains Pfam profiles PF00005: ABC transporter,
PF00664: ABC transporter transmembrane region
Length = 1488
Score = 28.3 bits (60), Expect = 5.2
Identities = 16/59 (27%), Positives = 27/59 (45%)
Frame = +1
Query: 334 CVELATLGELRLVERAAEVVLAPRDFASLRAHVKVASTENGVIFGNVVYEVTGASMDRG 510
C ++ L +++E + L RD ++ V ENG N+V+E G M +G
Sbjct: 1429 CDKILVLSSGQVLEYDSPQELLSRDTSAFFKMVHSTGPENGQYLSNLVFERRGNGMSQG 1487
>At4g34570.1 68417.m04912 bifunctional dihydrofolate
reductase-thymidylate synthase 2 / DHFR-TS (THY-2)
identical to SP|Q05763
Length = 565
Score = 27.9 bits (59), Expect = 6.8
Identities = 19/46 (41%), Positives = 23/46 (50%)
Frame = -3
Query: 200 LPRSIASPPLRPCSALLSASSNMSCCEPEAPAPASCENVMPVSIST 63
L S + PL SALL S C +P P+S N+ VSIST
Sbjct: 4 LQNSAKTLPLAFKSALLPLSQRWFC--KFSPKPSSLTNIFKVSIST 47
>At4g21130.1 68417.m03055 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400); some
similarity to a group of proteins with homology to
mammalian apoptosis regulators identified in zebrafish
(PUBMED:10917738)Apaf-1(gi:7677507)
Length = 537
Score = 27.5 bits (58), Expect = 9.0
Identities = 8/20 (40%), Positives = 10/20 (50%)
Frame = +2
Query: 338 WSWPRWASCAWWSARPRWCW 397
W ++ C WS RWCW
Sbjct: 454 WVCELFSFCKIWSVSDRWCW 473
>At3g15030.2 68416.m01902 TCP family transcription factor, putative
similar to TCP3 GB:AAC24010 [Arabidopsis thaliana]
Length = 420
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 618 QGLREHQHDGLA*VPAAPSCLHQH 689
QG E QHDGL P++ S + +H
Sbjct: 397 QGQEEEQHDGLTHKPSSASSISRH 420
>At3g15030.1 68416.m01901 TCP family transcription factor, putative
similar to TCP3 GB:AAC24010 [Arabidopsis thaliana]
Length = 420
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 618 QGLREHQHDGLA*VPAAPSCLHQH 689
QG E QHDGL P++ S + +H
Sbjct: 397 QGQEEEQHDGLTHKPSSASSISRH 420
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,768,405
Number of Sequences: 28952
Number of extensions: 186847
Number of successful extensions: 763
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 739
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 761
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -