Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1286
         (650 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase Cdc2|Sch...    40   3e-04
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po...    36   0.004
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy...    35   0.009
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo...    33   0.027
SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch...    32   0.062
SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33 |Schizo...    30   0.25 
SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha...    27   2.3  
SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subuni...    26   4.1  
SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po...    26   5.4  
SPAC22A12.13 |mug84||pig-P |Schizosaccharomyces pombe|chr 1|||Ma...    25   7.2  
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch...    25   7.2  
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   9.5  
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual    25   9.5  

>SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase
           Cdc2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 31/107 (28%), Positives = 51/107 (47%), Gaps = 8/107 (7%)
 Frame = +1

Query: 55  GTFYKVRHKVTNNIFAWKAYDCSAFSDEQIQNVVNEVKIISQVLS----ANLIRYYDTIL 222
           G  YK RHK++  I A K       S+      + E+ ++ +V      +N +R  D IL
Sbjct: 16  GVVYKARHKLSGRIVAMKKIRLEDESEGVPSTAIREISLLKEVNDENNRSNCVRLLD-IL 74

Query: 223 HSPSKTLYFVLEY--NSWRNVQELIEECKAT--DKHIEESFIWRLLH 351
           H+ SK LY V E+     +   + I E  AT  D  + + F ++L++
Sbjct: 75  HAESK-LYLVFEFLDMDLKKYMDRISETGATSLDPRLVQKFTYQLVN 120


>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 639

 Score = 36.3 bits (80), Expect = 0.004
 Identities = 23/93 (24%), Positives = 47/93 (50%), Gaps = 2/93 (2%)
 Frame = +1

Query: 1   ARMSEKLKDFQVLDVFCGGTFYKVRHKVTNNIFAWKAYDCSAFSDEQIQNVVNEVKIISQ 180
           +R  E  +++ +L     G+ Y VR+K+    +A K  + S F       +  E+K +++
Sbjct: 165 SRYIEDFEEYSLLGRGGFGSVYHVRNKIDGAEYAMKKIN-STFQQMSYSKIFREIKCLAK 223

Query: 181 VLSANLIRYYDTILHSPSKT--LYFVLEYNSWR 273
           +   N+IRY+ + + S ++   +  V+  NS R
Sbjct: 224 MDHPNVIRYFSSWVESTTEATQMPIVMSKNSSR 256


>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 589

 Score = 35.1 bits (77), Expect = 0.009
 Identities = 15/57 (26%), Positives = 35/57 (61%)
 Frame = +1

Query: 151 VVNEVKIISQVLSANLIRYYDTILHSPSKTLYFVLEYNSWRNVQELIEECKATDKHI 321
           ++NE+ ++ + +  N++ Y D+ L    + L+ V+EY +  ++ ++IE+ K T+  I
Sbjct: 356 ILNEITVMRESIHPNIVTYLDSFL-VRERHLWVVMEYMNAGSLTDIIEKSKLTEAQI 411


>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
            Wis4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1401

 Score = 33.5 bits (73), Expect = 0.027
 Identities = 14/76 (18%), Positives = 42/76 (55%)
 Frame = +1

Query: 142  IQNVVNEVKIISQVLSANLIRYYDTILHSPSKTLYFVLEYNSWRNVQELIEECKATDKHI 321
            +  + NE+ ++ ++   N++ YY   +H   + +Y  +E+    ++ +L+   +  D+++
Sbjct: 1080 VDQIHNEMTVLERLNHPNVVTYYGVEVH--REKVYIFMEFCQGGSLADLLAHGRIEDENV 1137

Query: 322  EESFIWRLLHELARVY 369
             + ++ +LL  LA ++
Sbjct: 1138 LKVYVVQLLEGLAYIH 1153


>SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 593

 Score = 32.3 bits (70), Expect = 0.062
 Identities = 20/69 (28%), Positives = 32/69 (46%)
 Frame = +1

Query: 55  GTFYKVRHKVTNNIFAWKAYDCSAFSDEQIQNVVNEVKIISQVLSANLIRYYDTILHSPS 234
           G  YK  + VT ++ A K        D      V EVKI+ ++   N++R  + ++   S
Sbjct: 289 GKVYKAINTVTGDLVALKRIRLEQEKDGFPITTVREVKILQRLRHKNIVRLLEIMVEKSS 348

Query: 235 KTLYFVLEY 261
             +Y V EY
Sbjct: 349 --VYMVFEY 355


>SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 338

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 3/69 (4%)
 Frame = +1

Query: 13  EKLKDFQVLDVFCGG---TFYKVRHKVTNNIFAWKAYDCSAFSDEQIQNVVNEVKIISQV 183
           E +  F++ +V   G   T + V+H+ T   FA K  +     + +I+ + NEV ++  +
Sbjct: 25  ESVSQFKIRNVIGKGSWSTVFVVKHRQTKQRFALKCINKKKHDEHKIKKLRNEVLLLQSL 84

Query: 184 LSANLIRYY 210
               L ++Y
Sbjct: 85  NHPLLCKFY 93


>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 19/69 (27%), Positives = 32/69 (46%)
 Frame = +1

Query: 55  GTFYKVRHKVTNNIFAWKAYDCSAFSDEQIQNVVNEVKIISQVLSANLIRYYDTILHSPS 234
           G+  K R + T  IFA K    +  +  Q Q ++ E+KI     S  +++YY    ++  
Sbjct: 91  GSVRKCRIRGTQMIFAMKTVLAAPNTALQKQ-LLRELKINRSCTSPYIVKYYGACYNNAE 149

Query: 235 KTLYFVLEY 261
             L   +EY
Sbjct: 150 CQLNIAMEY 158


>SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subunit
           Apc5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 737

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 10/18 (55%), Positives = 12/18 (66%)
 Frame = +1

Query: 193 NLIRYYDTILHSPSKTLY 246
           NL RY+D I+HS  K  Y
Sbjct: 275 NLCRYFDHIMHSDEKVSY 292


>SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 365

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 12/39 (30%), Positives = 21/39 (53%)
 Frame = +2

Query: 497 DVMRQVGDLIHTICYTPNACDKIKEFHYSDDLRDVISLL 613
           DVMR+ G+   T  +  N    + EF   +D+R+ ++ L
Sbjct: 116 DVMRKAGEPTFTDAHRENPGAGVVEFSTEEDMRNALTSL 154


>SPAC22A12.13 |mug84||pig-P |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 120

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +3

Query: 21  KGFSSFRCLLRRYVL*SETQGHQQHFRVEGVRL 119
           K FSS  C++ +Y L  E  G   H RV  +RL
Sbjct: 78  KPFSSLECIVDQYALVGEEDG-AAHGRVVDLRL 109


>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
            Slh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1935

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 28/121 (23%), Positives = 50/121 (41%), Gaps = 1/121 (0%)
 Frame = +1

Query: 118  CSAFSDEQIQNVVNEVKIISQVL-SANLIRYYDTILHSPSKTLYFVLEYNSWRNVQELIE 294
            CS FS  QI++  NE + +  ++ +++  +  D+I ++  K    +  Y S  +V++   
Sbjct: 827  CSEFS--QIKSRENEHRELESLMENSSPCQLRDSISNTSGKVNVILQSYISRAHVEDFTL 884

Query: 295  ECKATDKHIEESFIWRLLHELARVYKSVEEVNTVVLQKCLTPESVFVQRKWRTSHQLFQF 474
                         I R L E+A           + L K +       +R+W   H L QF
Sbjct: 885  TSDTNYVAQNAGRITRALFEIAMSRTWASAFTILSLNKSID------RRQWSFEHPLLQF 938

Query: 475  D 477
            D
Sbjct: 939  D 939


>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 780

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 15/50 (30%), Positives = 26/50 (52%)
 Frame = +1

Query: 157 NEVKIISQVLSANLIRYYDTILHSPSKTLYFVLEYNSWRNVQELIEECKA 306
           N  K+  Q  S+N I        SPS     + E+ S+ N+++L+++C A
Sbjct: 683 NICKLSLQKFSSNAIEQCIRTA-SPSTREQMLQEFLSFPNIEQLLDDCYA 731


>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1339

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 24/101 (23%), Positives = 47/101 (46%), Gaps = 11/101 (10%)
 Frame = +1

Query: 19  LKDFQVLDVFCGGTFYKVRHKVTNNIF-AWKAYDCSAFSDEQI-QNV------VNEVKII 174
           +KD  +L  F     +K RH++  +I+   K Y  S+ S  Q+ QN+       N+    
Sbjct: 15  IKDTDILSYFDANLPFKKRHQIVRSIYHGLKYYIYSSTSIIQVWQNIQDFISTKNDNAAF 74

Query: 175 SQVLSANLIRYYDTILH---SPSKTLYFVLEYNSWRNVQEL 288
            +++   ++RY  T  H       T +  +E + ++++ EL
Sbjct: 75  RELVYNLMMRYVSTQKHLSIIERHTFFQTIEKDPFQDIAEL 115


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,686,382
Number of Sequences: 5004
Number of extensions: 54591
Number of successful extensions: 172
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)