Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1277
(737 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 36 0.028
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 36 0.028
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 36 0.028
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.086
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 34 0.11
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.15
At5g16370.1 68418.m01913 AMP-binding protein, putative similar t... 31 1.1
At5g16340.1 68418.m01910 AMP-binding protein, putative similar t... 31 1.1
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 30 1.8
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 29 2.4
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 29 3.2
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 29 4.3
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 29 4.3
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 28 5.6
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 28 7.4
At4g31650.1 68417.m04495 transcriptional factor B3 family protei... 27 9.8
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 35.9 bits (79), Expect = 0.028
Identities = 13/73 (17%), Positives = 36/73 (49%), Gaps = 3/73 (4%)
Frame = +1
Query: 238 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYAL 408
I + + ++ D+ S E++ + D ++++G+ ++ + + R + R L
Sbjct: 131 IKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQL 190
Query: 409 PENCNPDTVESSV 447
P+NC D +++ +
Sbjct: 191 PDNCEKDKIKAEL 203
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 35.9 bits (79), Expect = 0.028
Identities = 38/138 (27%), Positives = 62/138 (44%), Gaps = 9/138 (6%)
Frame = +1
Query: 61 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMA-MANRDVGST 237
MSL+P G + + D +L D +TP ++ P K +A N V
Sbjct: 1 MSLVPSFFGGRRTNVFDP---FSLDVWDPFEGFLTPGLTN---APAKDVAAFTNAKVDWR 54
Query: 238 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFT 393
T F+ DV EE+ V+ DG ++ + G+ +EE+ D + R +F
Sbjct: 55 ETPEAHVFKA--DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFM 112
Query: 394 RRYALPENCNPDTVESSV 447
RR+ LPEN + V++S+
Sbjct: 113 RRFRLPENAKVEEVKASM 130
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 35.9 bits (79), Expect = 0.028
Identities = 36/137 (26%), Positives = 62/137 (45%), Gaps = 8/137 (5%)
Frame = +1
Query: 61 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMAMANRDVGSTI 240
MSL+P I G + + D +L D +TP+ + A N V
Sbjct: 1 MSLIPSIFGGRRTNVFDP---FSLDVFDPFEGFLTPSGLANA-PAMDVAAFTNAKVDWRE 56
Query: 241 TSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTR 396
T F+ D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTR
Sbjct: 57 TPEAHVFKA--DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTR 114
Query: 397 RYALPENCNPDTVESSV 447
R+ LPEN + +++S+
Sbjct: 115 RFRLPENAKMEEIKASM 131
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.3 bits (75), Expect = 0.086
Identities = 22/66 (33%), Positives = 36/66 (54%), Gaps = 8/66 (12%)
Frame = +1
Query: 274 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 429
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 430 TVESSV 447
V++S+
Sbjct: 124 QVKASM 129
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 33.9 bits (74), Expect = 0.11
Identities = 27/88 (30%), Positives = 44/88 (50%), Gaps = 8/88 (9%)
Frame = +1
Query: 208 AMANRDVGSTITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHG 372
A+AN V T+ F+ D+ EE+ V+ D V+ + G+ EE+QD
Sbjct: 42 AIANARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 99
Query: 373 YISRQ---FTRRYALPENCNPDTVESSV 447
+ R F+R++ LPEN D V++S+
Sbjct: 100 RVERSSGGFSRKFRLPENVKMDQVKASM 127
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.5 bits (73), Expect = 0.15
Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 8/66 (12%)
Frame = +1
Query: 274 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 429
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 430 TVESSV 447
V++++
Sbjct: 122 QVKAAM 127
>At5g16370.1 68418.m01913 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA adenosine monophosphate binding protein 5 AMPBP5
(AMPBP5) GI:20799718
Length = 552
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/40 (35%), Positives = 28/40 (70%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPIT 526
CL+ A+ +LS++ + V++V++P TPA + + AVP++
Sbjct: 50 CLRVAS-SLSSIGIGRSDVVSVLSPNTPAMYELQFAVPMS 88
>At5g16340.1 68418.m01910 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
adenosine monophosphate binding protein 6 AMPBP6
(AMPBP6) GI:20799720
Length = 550
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/40 (35%), Positives = 28/40 (70%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPIT 526
CL+ A+ +LS++ + V++V++P TPA + + AVP++
Sbjct: 50 CLRVAS-SLSSIGIGRSDVVSVLSPNTPAMYELQFAVPMS 88
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 29.9 bits (64), Expect = 1.8
Identities = 19/66 (28%), Positives = 36/66 (54%), Gaps = 8/66 (12%)
Frame = +1
Query: 274 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 429
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 430 TVESSV 447
V++++
Sbjct: 128 EVKATM 133
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 29.5 bits (63), Expect = 2.4
Identities = 15/39 (38%), Positives = 25/39 (64%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPI 523
C + A +L +L +S + V++V+AP TPA + AVP+
Sbjct: 49 CCRLAA-SLISLNISKNDVVSVMAPNTPALYEMHFAVPM 86
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 29.1 bits (62), Expect = 3.2
Identities = 14/39 (35%), Positives = 25/39 (64%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPI 523
C + A +L +L ++ + V++V+AP TPA + AVP+
Sbjct: 49 CCRLAA-SLISLNIAKNDVVSVVAPNTPAIYEMHFAVPM 86
>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 2 AMPBP2 (AMPBP2) GI:20799712
Length = 603
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPI 523
CL+ A+ L+NL +S V+ +AP PA + AVP+
Sbjct: 96 CLRLAS-ALTNLGISRGDVVAALAPNVPAMHELHFAVPM 133
>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 12 (At1g65890) mRNA
GI:29893228, acyl-activating enzyme 12 [Arabidopsis
thaliana] GI:29893229
Length = 578
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPI 523
C + A +L +L + + V++V+AP TPA + AVP+
Sbjct: 49 CCRLAA-SLISLNIGKNDVVSVVAPNTPAMYEMHFAVPM 86
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 28.3 bits (60), Expect = 5.6
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 407 CLKTATLTLSNLRLSSDGVLTVIAPRTPAATKNERAVPI 523
C++ A+ LS L +S+ V++V+AP PA + VP+
Sbjct: 51 CVRIAS-ALSQLGISTGDVVSVLAPNVPAMVELHFGVPM 88
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/49 (30%), Positives = 21/49 (42%)
Frame = +1
Query: 295 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVES 441
+EI D Y EG EE ++ R + L E+C + VES
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVES 96
>At4g31650.1 68417.m04495 transcriptional factor B3 family protein
low similarity to reproductive meristem gene 1 from
[Brassica oleracea var. botrytis] GI:3170424,
[Arabidopsis thaliana] GI:13604227; contains Pfam
profile PF02362: B3 DNA binding domain
Length = 493
Score = 27.5 bits (58), Expect = 9.8
Identities = 22/77 (28%), Positives = 39/77 (50%), Gaps = 3/77 (3%)
Frame = -1
Query: 653 QIKLTHAYTF-LIHSVPRVIVLFRCSQLRPVGSLXLLPDRTRFE*WEQLARSSW--QPES 483
++++ + TF L+H+ ++ V CS+L G+ R R + W Q +RS + +
Sbjct: 201 KLEVGDSCTFKLLHNA-KMPVFRLCSKLPKAGAEARPLKRARVQRWSQESRSKHDVREKI 259
Query: 482 WERSRSARRQRTTEDST 432
E +RR RT+ ST
Sbjct: 260 AEEGEPSRRNRTSNKST 276
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,649,203
Number of Sequences: 28952
Number of extensions: 292984
Number of successful extensions: 878
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 862
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 878
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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