Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1275
(620 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44530.1 68415.m05539 ribose-phosphate pyrophosphokinase, put... 134 6e-32
At1g32380.1 68414.m03995 ribose-phosphate pyrophosphokinase 2 / ... 132 2e-31
At2g35390.2 68415.m04339 ribose-phosphate pyrophosphokinase 1 / ... 131 3e-31
At2g35390.1 68415.m04338 ribose-phosphate pyrophosphokinase 1 / ... 131 3e-31
At1g10700.1 68414.m01217 ribose-phosphate pyrophosphokinase 3 / ... 46 2e-05
At2g42910.1 68415.m05316 ribose-phosphate pyrophosphokinase 4 / ... 43 1e-04
At3g62020.1 68416.m06966 germin-like protein (GLP10) identical t... 32 0.35
At5g01940.1 68418.m00113 eukaryotic translation initiation facto... 30 1.4
At5g50640.1 68418.m06274 CBS domain-containing protein / octicos... 28 5.7
At5g50530.1 68418.m06258 CBS domain-containing protein / octicos... 28 5.7
At3g53900.2 68416.m05955 uracil phosphoribosyltransferase, putat... 28 5.7
At3g53900.1 68416.m05954 uracil phosphoribosyltransferase, putat... 28 5.7
At1g17980.2 68414.m02224 nucleotidyltransferase family protein c... 28 5.7
At1g17980.1 68414.m02225 nucleotidyltransferase family protein c... 28 5.7
At5g66160.2 68418.m08334 protease-associated zinc finger (C3HC4-... 27 7.6
At5g66160.1 68418.m08335 protease-associated zinc finger (C3HC4-... 27 7.6
At4g12440.2 68417.m01969 adenine phosphoribosyltransferase, puta... 27 7.6
At3g62420.1 68416.m07012 bZIP transcription factor family protei... 27 7.6
>At2g44530.1 68415.m05539 ribose-phosphate pyrophosphokinase,
putative / phosphoribosyl diphosphate synthetase,
putative very strong similarity to phosphoribosyl
pyrophosphate synthase [Spinacia oleracea] GI:4902849;
contains Pfam profile PF00156: Phosphoribosyl
transferase domain
Length = 394
Score = 134 bits (323), Expect = 6e-32
Identities = 67/169 (39%), Positives = 100/169 (59%), Gaps = 1/169 (0%)
Frame = +3
Query: 3 FDIPVDNLFAEPAVLKWIKENIPDWKTSIVVSPDAGGAKRVTSIADRLN-VEFALIHKER 179
FDIPVD+++ +P +L ++ + +VVSPD GG R + A +L+ A++ K R
Sbjct: 212 FDIPVDHVYGQPVILDYLASKAISSEDLVVVSPDVGGVARARAFAKKLSDAPLAIVDKRR 271
Query: 180 KKANEVASMVLVGDVKDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSGP 359
N M L+GDVK + AI+VDDM DT GTI A L + GA +VYA TH +FS P
Sbjct: 272 HGHNVAEVMNLIGDVKGKVAIMVDDMIDTAGTISKGAALLHQEGAREVYACTTHAVFSPP 331
Query: 360 AISRINNACLEAVVVTNTIPQERHMQECPKIQCIDVSMMLAEAVRRTHN 506
AISR+++ + V++TNTIP P++ + V+ +L E + R H+
Sbjct: 332 AISRLSSGLFQEVIITNTIPLS-EKNYFPQLTVLSVANLLGETIWRVHD 379
>At1g32380.1 68414.m03995 ribose-phosphate pyrophosphokinase 2 /
phosphoribosyl diphosphate synthetase 2 (PRS2) identical
to SP:Q42583 from [Arabidopsis thaliana]; strong
similarity to phosphoribosyl diphosphate synthetase 1
(ribose-phosphate pyrophosphokinase 1 (PRS I)
[Arabidopsis thaliana] GI:633140, SP|Q42581
Length = 400
Score = 132 bits (318), Expect = 2e-31
Identities = 66/169 (39%), Positives = 100/169 (59%), Gaps = 1/169 (0%)
Frame = +3
Query: 3 FDIPVDNLFAEPAVLKWIKENIPDWKTSIVVSPDAGGAKRVTSIADRLN-VEFALIHKER 179
FDIPVD+++ +P +L ++ + +VVSPD GG R + A +L+ A++ K R
Sbjct: 224 FDIPVDHVYCQPVILDYLASKSISSEDLVVVSPDVGGVARARAFAKKLSDAPLAIVDKRR 283
Query: 180 KKANEVASMVLVGDVKDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSGP 359
N M L+GDVK + A++VDD+ DT GTI A L E GA +VYA TH +FS P
Sbjct: 284 HGHNVAEVMNLIGDVKGKVAVMVDDIIDTAGTIVKGAALLHEEGAREVYACCTHAVFSPP 343
Query: 360 AISRINNACLEAVVVTNTIPQERHMQECPKIQCIDVSMMLAEAVRRTHN 506
AI R+++ L+ V+VTNT+P P++ + V+ +L E + R H+
Sbjct: 344 AIERLSSGLLQEVIVTNTLP-VAEKNYFPQLTILSVANLLGETIWRVHD 391
>At2g35390.2 68415.m04339 ribose-phosphate pyrophosphokinase 1 /
phosphoribosyl diphosphate synthetase 1 (PRSI) identical
to phosphoribosyl diphosphate synthetase 1
(ribose-phosphate pyrophosphokinase 1 (PRS I)
[Arabidopsis thaliana] GI:633140, SP|Q42581
Length = 403
Score = 131 bits (317), Expect = 3e-31
Identities = 68/170 (40%), Positives = 103/170 (60%), Gaps = 2/170 (1%)
Frame = +3
Query: 3 FDIPVDNLFAEPAVLKWI-KENIPDWKTSIVVSPDAGGAKRVTSIADRLN-VEFALIHKE 176
FDIPVD+++ +P +L ++ ++IP + +VVSPD GG R + A +L+ A++ K
Sbjct: 227 FDIPVDHVYCQPVILDYLASKSIPS-EDLVVVSPDVGGVARARAFAKKLSDAPLAIVDKR 285
Query: 177 RKKANEVASMVLVGDVKDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSG 356
R N M L+GDV+ + AI+VDDM DT GTI A L + GA +VYA TH +FS
Sbjct: 286 RSGHNVAEVMNLIGDVRGKVAIMVDDMIDTAGTIVKGAALLHQEGAREVYACCTHAVFSP 345
Query: 357 PAISRINNACLEAVVVTNTIPQERHMQECPKIQCIDVSMMLAEAVRRTHN 506
PAI R++ L+ V+VTNT+P P++ + V+ +L E + R H+
Sbjct: 346 PAIERLSGGLLQEVIVTNTLP-VAEKNYFPQLTILSVANLLGETIWRVHD 394
>At2g35390.1 68415.m04338 ribose-phosphate pyrophosphokinase 1 /
phosphoribosyl diphosphate synthetase 1 (PRSI) identical
to phosphoribosyl diphosphate synthetase 1
(ribose-phosphate pyrophosphokinase 1 (PRS I)
[Arabidopsis thaliana] GI:633140, SP|Q42581
Length = 352
Score = 131 bits (317), Expect = 3e-31
Identities = 68/170 (40%), Positives = 103/170 (60%), Gaps = 2/170 (1%)
Frame = +3
Query: 3 FDIPVDNLFAEPAVLKWI-KENIPDWKTSIVVSPDAGGAKRVTSIADRLN-VEFALIHKE 176
FDIPVD+++ +P +L ++ ++IP + +VVSPD GG R + A +L+ A++ K
Sbjct: 176 FDIPVDHVYCQPVILDYLASKSIPS-EDLVVVSPDVGGVARARAFAKKLSDAPLAIVDKR 234
Query: 177 RKKANEVASMVLVGDVKDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSG 356
R N M L+GDV+ + AI+VDDM DT GTI A L + GA +VYA TH +FS
Sbjct: 235 RSGHNVAEVMNLIGDVRGKVAIMVDDMIDTAGTIVKGAALLHQEGAREVYACCTHAVFSP 294
Query: 357 PAISRINNACLEAVVVTNTIPQERHMQECPKIQCIDVSMMLAEAVRRTHN 506
PAI R++ L+ V+VTNT+P P++ + V+ +L E + R H+
Sbjct: 295 PAIERLSGGLLQEVIVTNTLP-VAEKNYFPQLTILSVANLLGETIWRVHD 343
>At1g10700.1 68414.m01217 ribose-phosphate pyrophosphokinase 3 /
phosphoribosyl diphosphate synthetase 3 (PRS3) nearly
identical to phosphoribosyl diphosphate synthase
GI:4902470 from [Arabidopsis thaliana]
Length = 411
Score = 46.4 bits (105), Expect = 2e-05
Identities = 28/103 (27%), Positives = 50/103 (48%)
Frame = +3
Query: 42 VLKWIKENIPDWKTSIVVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGD 221
+LK +++PD + PD G KR +L ++ + + ++ + GD
Sbjct: 255 LLKSRLQSLPDSDNISIAFPDDGAWKRFHK---QLQHYPTIVCNKVRMGDKRIVRIKEGD 311
Query: 222 VKDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIF 350
+ R ++VDD+ + GT+ + L GA K+ A +THGIF
Sbjct: 312 AEGRHVVIVDDLVQSGGTLIECQKVLAAHGAAKISAYVTHGIF 354
>At2g42910.1 68415.m05316 ribose-phosphate pyrophosphokinase 4 /
phosphoribosyl diphosphate synthetase 4 (PRS4) identical
to phosphoribosyl diphosphate synthase (prs4)
[Arabidopsis thaliana] GI:4902472
Length = 337
Score = 43.2 bits (97), Expect = 1e-04
Identities = 27/97 (27%), Positives = 45/97 (46%)
Frame = +3
Query: 60 ENIPDWKTSIVVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGDVKDRTA 239
+ +P+ + IV PD G KR + D + K R+ + + G+
Sbjct: 188 QQLPETEKVIVAFPDDGAWKRFHKLLDHYPT--VVCTKVREGDKRIVRLK-EGNPAGCHV 244
Query: 240 ILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIF 350
++VDD+ + GT+ + L GA KV A +THG+F
Sbjct: 245 VIVDDLVQSGGTLIECQKVLAAHGAVKVSAYVTHGVF 281
>At3g62020.1 68416.m06966 germin-like protein (GLP10) identical to
germin-like protein subfamily 2 member 4 [SP|Q9M263]
Length = 220
Score = 31.9 bits (69), Expect = 0.35
Identities = 18/64 (28%), Positives = 29/64 (45%)
Frame = -1
Query: 200 GYFIGLLSLFVDQSKFNIQTIRNRCHPFSTTSIRGHNNACFPVRYVFFDPF*HCGFGKEV 21
G+F LLSL V ++ T+++ C T+ I+ + C P + F G GK
Sbjct: 7 GFFFTLLSLNVIVLAYDPDTLQDLCVADRTSGIKVNGFTCKPESNITASDFFFAGIGKPA 66
Query: 20 IYRN 9
+ N
Sbjct: 67 VVNN 70
>At5g01940.1 68418.m00113 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 231
Score = 29.9 bits (64), Expect = 1.4
Identities = 19/74 (25%), Positives = 36/74 (48%)
Frame = +3
Query: 57 KENIPDWKTSIVVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGDVKDRT 236
++N+PD ++S PD G + ++ + DRL E + ER + + +L + T
Sbjct: 57 EDNLPDCQSSRKFEPDYGYKELLSMVFDRLREEDVEVSTERPRTVMMPPQLLA----EGT 112
Query: 237 AILVDDMADTCGTI 278
+ + AD C T+
Sbjct: 113 ITVCLNFADLCRTM 126
>At5g50640.1 68418.m06274 CBS domain-containing protein /
octicosapeptide/Phox/Bemp1 (PB1) domain-containing
protein contains Pfam profiles: PF00571 CBS domain,
PF00564: PB1 domain
Length = 548
Score = 27.9 bits (59), Expect = 5.7
Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Frame = +3
Query: 399 VVTNTIPQERHMQE---CPKIQCIDVSMMLAEAVRRTHNGGICFIPLL 533
V++ +PQE E P + V M + EA+ HNG +P+L
Sbjct: 285 VISQNLPQETTTVEKVMTPNPESATVDMAIVEALHIMHNGKFLHLPVL 332
>At5g50530.1 68418.m06258 CBS domain-containing protein /
octicosapeptide/Phox/Bemp1 (PB1) domain-containing
protein contains Pfam profiles: PF00571 CBS domain,
PF00564: PB1 domain
Length = 548
Score = 27.9 bits (59), Expect = 5.7
Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Frame = +3
Query: 399 VVTNTIPQERHMQE---CPKIQCIDVSMMLAEAVRRTHNGGICFIPLL 533
V++ +PQE E P + V M + EA+ HNG +P+L
Sbjct: 285 VISQNLPQETTTVEKVMTPNPESATVDMAIVEALHIMHNGKFLHLPVL 332
>At3g53900.2 68416.m05955 uracil phosphoribosyltransferase, putative
/ UMP pyrophosphorylase, putative / UPRTase, putative
similar to SP|P50926 Uracil phosphoribosyltransferase
(EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase)
{Lactococcus lactis}; contains Pfam profile PF00156:
Phosphoribosyl transferase domain
Length = 296
Score = 27.9 bits (59), Expect = 5.7
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = +3
Query: 225 KDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSGPAISRIN 377
K+ LVD + T GTI A + L E G + + I + PA+S++N
Sbjct: 203 KNSRVFLVDPVLATGGTIMAAMDLLKERGLSVQQIKVICAIAAPPALSKLN 253
>At3g53900.1 68416.m05954 uracil phosphoribosyltransferase, putative
/ UMP pyrophosphorylase, putative / UPRTase, putative
similar to SP|P50926 Uracil phosphoribosyltransferase
(EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase)
{Lactococcus lactis}; contains Pfam profile PF00156:
Phosphoribosyl transferase domain
Length = 231
Score = 27.9 bits (59), Expect = 5.7
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = +3
Query: 225 KDRTAILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSGPAISRIN 377
K+ LVD + T GTI A + L E G + + I + PA+S++N
Sbjct: 138 KNSRVFLVDPVLATGGTIMAAMDLLKERGLSVQQIKVICAIAAPPALSKLN 188
>At1g17980.2 68414.m02224 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 586
Score = 27.9 bits (59), Expect = 5.7
Identities = 21/55 (38%), Positives = 27/55 (49%)
Frame = -2
Query: 355 PEKIP*VKIAYTLVAPASINFSAAWQIVPQVSAMSSTKMAVLSLTSPTSTIEATS 191
PE +P KIA T A + ++ QV S K+AVLS T+ EATS
Sbjct: 484 PESLPIEKIA-TPQAHETEELEESFDFGNQVIEQISHKVAVLSATATIPPFEATS 537
>At1g17980.1 68414.m02225 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 713
Score = 27.9 bits (59), Expect = 5.7
Identities = 21/55 (38%), Positives = 27/55 (49%)
Frame = -2
Query: 355 PEKIP*VKIAYTLVAPASINFSAAWQIVPQVSAMSSTKMAVLSLTSPTSTIEATS 191
PE +P KIA T A + ++ QV S K+AVLS T+ EATS
Sbjct: 611 PESLPIEKIA-TPQAHETEELEESFDFGNQVIEQISHKVAVLSATATIPPFEATS 664
>At5g66160.2 68418.m08334 protease-associated zinc finger
(C3HC4-type RING finger) family protein contains Pfam
domain, PF02225: protease-associated (PA) domain and
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger); similar to ReMembR-H2 protein JR702
[Arabidopsis thaliana] gi|6942149|gb|AAF32326; identical
to cDNA ReMembR-H2 protein JR700 mRNA, complete cds
GI:6942146
Length = 290
Score = 27.5 bits (58), Expect = 7.6
Identities = 31/121 (25%), Positives = 51/121 (42%), Gaps = 9/121 (7%)
Frame = +3
Query: 237 AILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFS-GPAISRINNACLEAVVVTNT 413
A+ V D D C + HAA T +A++ G S + N+ +AV+V +
Sbjct: 55 ALYVADPLDGCSPLLHAAASNWTQHRTTKFALIIRGECSFEDKLLNAQNSGFQAVIVYDN 114
Query: 414 IPQER--HMQECPK---IQCIDVSMMLAEAVR---RTHNGGICFIPLL*CSILVILYFTF 569
I E M+ P+ + + VS + E +R R +G C P S +L +F
Sbjct: 115 IDNEDLIVMKVNPQDITVDAVFVSNVAGEILRKYARGRDGECCLNPPDRGSAWTVLAISF 174
Query: 570 Y 572
+
Sbjct: 175 F 175
>At5g66160.1 68418.m08335 protease-associated zinc finger
(C3HC4-type RING finger) family protein contains Pfam
domain, PF02225: protease-associated (PA) domain and
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger); similar to ReMembR-H2 protein JR702
[Arabidopsis thaliana] gi|6942149|gb|AAF32326; identical
to cDNA ReMembR-H2 protein JR700 mRNA, complete cds
GI:6942146
Length = 310
Score = 27.5 bits (58), Expect = 7.6
Identities = 31/121 (25%), Positives = 51/121 (42%), Gaps = 9/121 (7%)
Frame = +3
Query: 237 AILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFS-GPAISRINNACLEAVVVTNT 413
A+ V D D C + HAA T +A++ G S + N+ +AV+V +
Sbjct: 55 ALYVADPLDGCSPLLHAAASNWTQHRTTKFALIIRGECSFEDKLLNAQNSGFQAVIVYDN 114
Query: 414 IPQER--HMQECPK---IQCIDVSMMLAEAVR---RTHNGGICFIPLL*CSILVILYFTF 569
I E M+ P+ + + VS + E +R R +G C P S +L +F
Sbjct: 115 IDNEDLIVMKVNPQDITVDAVFVSNVAGEILRKYARGRDGECCLNPPDRGSAWTVLAISF 174
Query: 570 Y 572
+
Sbjct: 175 F 175
>At4g12440.2 68417.m01969 adenine phosphoribosyltransferase,
putative strong similarity to SP|P31166 Adenine
phosphoribosyltransferase 1 (EC 2.4.2.7) (APRT)
{Arabidopsis thaliana}; contains Pfam profile PF00156:
Phosphoribosyl transferase domain
Length = 182
Score = 27.5 bits (58), Expect = 7.6
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = +3
Query: 219 DVKDRTAILVDDMADTCGTICHAAEKLIEAGA 314
D DR A++VDD+ T GT+C A L GA
Sbjct: 120 DSGDR-ALVVDDLIATGGTLCAAMNLLKRVGA 150
>At3g62420.1 68416.m07012 bZIP transcription factor family protein
similar to common plant regulatory factor 6 GI:9650826
from [Petroselinum crispum]
Length = 146
Score = 27.5 bits (58), Expect = 7.6
Identities = 14/70 (20%), Positives = 33/70 (47%)
Frame = +3
Query: 96 SPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGDVKDRTAILVDDMADTCGT 275
SP++ R ++ D + + ++E + + + +GD+ + +L +D A
Sbjct: 9 SPESDNDPRYATVTDERKRKRMISNRESARRSRMRKQKQLGDLINEVTLLKNDNAKITEQ 68
Query: 276 ICHAAEKLIE 305
+ A++K IE
Sbjct: 69 VDEASKKYIE 78
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,223,180
Number of Sequences: 28952
Number of extensions: 306184
Number of successful extensions: 926
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 890
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 921
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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