BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-1274 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC553.12c ||SPCC794.13|conserved fungal protein|Schizosaccharo... 27 2.0 SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||... 26 4.5 SPAC1782.02c |||conserved fungal protein|Schizosaccharomyces pom... 26 4.5 SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 25 7.9 SPAC977.10 |sod2||CPA1 sodium ion/proton antiporter |Schizosacch... 25 7.9 >SPCC553.12c ||SPCC794.13|conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 521 Score = 27.5 bits (58), Expect = 2.0 Identities = 16/46 (34%), Positives = 22/46 (47%) Frame = +2 Query: 80 FRLNFISLTL*GIDEYVTYSDCIDINVYLLCIEKNDYY*IFIGRSL 217 FRLN + +D YV Y + N Y+ C+ N F+G SL Sbjct: 383 FRLNVLMTK--SMDSYVVYRKNMGRNFYVKCVASNVSMLSFLGWSL 426 >SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 565 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/41 (26%), Positives = 23/41 (56%) Frame = -2 Query: 165 RYTFISIQSEYVTYSSIPHSVNDIKFNLNYNTGELSIRSQN 43 + T ++ E+V +++ HS+ D F+ N + L I +Q+ Sbjct: 365 KVTLTNLPYEFVRFNNSMHSIGDNNFSYNNSEAVLQIFAQS 405 >SPAC1782.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +1 Query: 430 FRSIILFYCVTVLLNRCGVRGEVTFVTHSRLKIMI 534 FR+I+LFYC + G FV S L +I Sbjct: 5 FRAIVLFYCCMLTFAGIGFLWNPKFVVESGLVALI 39 >SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ubp21|Schizosaccharomyces pombe|chr 2|||Manual Length = 1129 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/43 (27%), Positives = 26/43 (60%), Gaps = 4/43 (9%) Frame = -3 Query: 533 IIIFRRECVTNVTSPRT----PQRFNNTVTQ*NKIMERKSMEQ 417 ++ FR+ + ++ SP T P NT+ + ++++ERK +E+ Sbjct: 540 LVYFRKSRLDHILSPVTAEDVPFHVRNTLDEEHRVVERKLLER 582 >SPAC977.10 |sod2||CPA1 sodium ion/proton antiporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 468 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +3 Query: 324 C*YGVFDGETVGYKCIVINQRA*ITRFRDFILFHRFPFHYFILLCNSV 467 C +G+F G +GY I + A R D I ++ P LLC+ + Sbjct: 213 CAFGIFFGCVIGYLLSFILKHAQKYRLIDAISYYSLPL-AIPLLCSGI 259 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,693,793 Number of Sequences: 5004 Number of extensions: 54776 Number of successful extensions: 108 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 108 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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