Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1267
(722 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 219 4e-58
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 213 3e-56
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 33 0.031
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.13
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 0.51
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 29 0.67
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 28 1.2
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 28 1.2
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 2.7
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 3.6
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 27 3.6
SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 26 4.7
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 6.3
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 25 8.3
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 8.3
SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|c... 25 8.3
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 8.3
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 219 bits (534), Expect = 4e-58
Identities = 107/177 (60%), Positives = 133/177 (75%), Gaps = 2/177 (1%)
Frame = +2
Query: 191 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 370
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 371 RVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVF 544
R++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVF
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 545 YLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNF 715
Y KMKGDYYRYLAE A GE R + S + Y+ A EI+ A++ PTHPIRLGLALNF
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNF 179
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 213 bits (519), Expect = 3e-56
Identities = 106/178 (59%), Positives = 133/178 (74%), Gaps = 2/178 (1%)
Frame = +2
Query: 188 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 367
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 368 WRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKV 541
WR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKV
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120
Query: 542 FYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNF 715
FY KMKGDYYRYLAE GE + S +AY+ A +I+ A++ PT P+RLGLALNF
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNF 178
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 33.5 bits (73), Expect = 0.031
Identities = 25/104 (24%), Positives = 44/104 (42%)
Frame = +2
Query: 164 ISSLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKN 343
I S+ S S EEL+ R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2998 IMSITMSDSSAYGEELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 344 VVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREIC 475
+R+ + +E+ + EY +E+ L++ C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.13
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
Frame = +2
Query: 191 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 361
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 362 SSWRVIS 382
S+W S
Sbjct: 62 STWATFS 68
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 29.5 bits (63), Expect = 0.51
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = +2
Query: 197 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 376
+K+E QR + Q R D M + +G S+ + SV+ + +R S+
Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374
Query: 377 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 466
S E + E SE R+Q++ +E R + EK+LR
Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 29.1 bits (62), Expect = 0.67
Identities = 36/135 (26%), Positives = 64/135 (47%), Gaps = 4/135 (2%)
Frame = +2
Query: 320 LLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYR-VKVEKELR-EICYDVLGL 493
L+S +N A RS + + I + S + ++ + YR VK K L +IC+ +
Sbjct: 968 LVSYVRRNAETAERS--QALPFITRNL-ASIKSHELHEAYRLVKANKILEAQICFRSIIY 1024
Query: 494 LDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAK 670
L L A++ E + + RY+ ++ E R ED+++A + ++ + A
Sbjct: 1025 LA--LTTVANSEEEADEISALIDECCRYIVALSCELERRRLGEEDTKRALELSYYFASAD 1082
Query: 671 MQPTHP-IRLGLALN 712
+QP H I L LA+N
Sbjct: 1083 LQPMHSIIALRLAIN 1097
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 28.3 bits (60), Expect = 1.2
Identities = 17/59 (28%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
Frame = +3
Query: 78 KANAFECSRHIEICFVPSANANSIRG-ISELVLFHRPRCPSTRKNWCNVPNWPNKLSDM 251
K N+ C + E+ V S+N + S+ + F +PR K N WPN+ S +
Sbjct: 42 KENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETSHL 100
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 28.3 bits (60), Expect = 1.2
Identities = 29/100 (29%), Positives = 51/100 (51%), Gaps = 4/100 (4%)
Frame = +2
Query: 389 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 565
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 566 YYR-YLAEVATGETRHSVV--EDSQKAYQDAFEISKAKMQ 676
+R Y+ E+ + ++ E KA +D E SK ++Q
Sbjct: 100 SFRDYVGELNLLKELCKMINEEPELKAIEDT-EESKKQLQ 138
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 2.7
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +2
Query: 491 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 628
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 338 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 454
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 3.6
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 3/110 (2%)
Frame = +2
Query: 197 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 367
D ++RA A QA E+ + + +K E+ +L LL V + + R S
Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322
Query: 368 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 517
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/45 (31%), Positives = 19/45 (42%)
Frame = +3
Query: 114 ICFVPSANANSIRGISELVLFHRPRCPSTRKNWCNVPNWPNKLSD 248
I V +A S+ I EL P+C + N P W + L D
Sbjct: 75 ISSVKAAYGRSMEEIIELKGVELPKCLPKHARYHNAPKWRSSLMD 119
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 326 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 460
S +Y + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 25.4 bits (53), Expect = 8.3
Identities = 30/111 (27%), Positives = 45/111 (40%), Gaps = 7/111 (6%)
Frame = +2
Query: 200 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVG-ARRSSWRV 376
KE L +L E + D + A + V NLL + YKNV A +
Sbjct: 608 KEHLYSFLQLVEPSFAKSDSSNATESQISESVRKGISIFNLLFIVYKNVCSQAGINPSTK 667
Query: 377 ISSIEQKTEGSE------RKQQMAKEYRVKVEKELREICYDVLGLLDKHLI 511
+ +++ T E + Q +EY+ K E ELR + LL+ LI
Sbjct: 668 LEDLDEHTLSDELTYITKKFVQKDQEYQTK-EIELRNYKITLQSLLEDKLI 717
>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 85
Score = 25.4 bits (53), Expect = 8.3
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -2
Query: 682 CGLHFRLADFKSILVCFL*ILYNRMSGFSCGHFC 581
C L+F L + K+ L+ I+Y + GFS H C
Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75
>SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1077
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = -1
Query: 68 HKNRQFCRKGKNVFEPANLSTI 3
+K RQ C N F P +LSTI
Sbjct: 232 YKARQICNDIMNTFHPNDLSTI 253
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 8.3
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 410 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 532
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,034,048
Number of Sequences: 5004
Number of extensions: 60349
Number of successful extensions: 198
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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