Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-1262
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 40 4e-04
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 40 4e-04
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 37 0.002
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 35 0.011
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 34 0.015
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 33 0.026
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 32 0.060
SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 32 0.079
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 31 0.18
SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 30 0.32
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 0.97
SPAC19A8.03 |||phosphatidylinositol-3-phosphatase |Schizosacchar... 28 1.3
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 2.3
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 26 3.9
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.2
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 26 5.2
SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosacchar... 26 5.2
SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizos... 26 5.2
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 25 6.9
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 25 6.9
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 25 6.9
SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb... 25 6.9
SPBC36B7.05c |||phosphatidylinositol|Schizosaccharomyces pombe|c... 25 9.1
SPAC227.09 |||folylpolyglutamate synthase|Schizosaccharomyces po... 25 9.1
SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|... 25 9.1
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 39.5 bits (88), Expect = 4e-04
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +3
Query: 450 KKHLDQLKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQCCYG 587
++ L+ ++P + + C C+E I P +CGHIFC C G
Sbjct: 237 ERDLEDKNKLPFIPEGNRKCSLCMEFIHCPAATECGHIFCWSCING 282
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 39.5 bits (88), Expect = 4e-04
Identities = 25/98 (25%), Positives = 39/98 (39%), Gaps = 1/98 (1%)
Frame = +3
Query: 303 PLQIGKNLTGIDYVHVRPAATASYAHLNLLGYITLLHAFVCCGQSVYLAKKHLDQLKEIP 482
PL++ + + D + P T++ + LL H + S A ++++
Sbjct: 102 PLELKEEICHDDCLSSSPPCTSALTEITLLP--PTFHNLIPSSSSYETAVAEFLHMEDLL 159
Query: 483 NE-VDNEKTCVACLEEILQPCVLQCGHIFCHQCCYGAL 593
E V E C C + P V CGH FC C AL
Sbjct: 160 QENVSRELECQICFGMLYDPVVSPCGHTFCGPCLMQAL 197
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 37.1 bits (82), Expect = 0.002
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +3
Query: 459 LDQLKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQC 578
+D KE +E +E+ C C E I CVL C H CH C
Sbjct: 67 VDASKEEQDE--DEQICFICAEGITYSCVLPCNHRMCHVC 104
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 34.7 bits (76), Expect = 0.011
Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = +3
Query: 450 KKHLDQLKE-IPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQCCYGALK--TVVHWCRT 620
+++L L E I + ++ + C+ C + I Q + CGH++C C LK + C+T
Sbjct: 1072 RRYLTNLYEHIVLKAESHQICIICRDIIKQGFITTCGHLYCSFCLEAWLKHSSSCPMCKT 1131
Query: 621 PFTK 632
K
Sbjct: 1132 KLNK 1135
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 34.3 bits (75), Expect = 0.015
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = +3
Query: 468 LKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQCCYGALK 596
L E ++ C C E + +P CGH +C++C LK
Sbjct: 72 LLETFQKIKKTLECPICTEALQRPFTTHCGHTYCYECLLNWLK 114
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 33.5 bits (73), Expect = 0.026
Identities = 15/39 (38%), Positives = 18/39 (46%)
Frame = +3
Query: 507 CVACLEEILQPCVLQCGHIFCHQCCYGALKTVVHWCRTP 623
CV CL+ CGHIFC+ C AL T + P
Sbjct: 206 CVICLDSPENLSCTPCGHIFCNFCILSALGTTAATQKCP 244
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 32.3 bits (70), Expect = 0.060
Identities = 23/86 (26%), Positives = 35/86 (40%), Gaps = 9/86 (10%)
Frame = +3
Query: 402 TLLHAFVCCGQSVYLAKKHLDQLKEIPNEVDNEKTCVACLEEILQPCVLQCGHIFCHQCC 581
+L++ FV G+ + +D LK + C C E I P +L C H C C
Sbjct: 845 SLINQFVVTGKPIPSDILKIDTLKSFEALITECPIC--CNEPIQNPLLLNCKHACCGDCL 902
Query: 582 -----YGALKTVV----HWCRTPFTK 632
Y + ++ H CR PF +
Sbjct: 903 SEHIQYQKRRNIIPPLCHTCRQPFNE 928
>SPBC13E7.02 |cwf24||GCN5-related N
acetyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 533
Score = 31.9 bits (69), Expect = 0.079
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +3
Query: 507 CVACLEEILQPCVLQCGHIFCHQC 578
C+ C ++ P CGH FC QC
Sbjct: 254 CLICKKDYRSPIATTCGHHFCEQC 277
>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 30.7 bits (66), Expect = 0.18
Identities = 14/47 (29%), Positives = 20/47 (42%), Gaps = 2/47 (4%)
Frame = +3
Query: 489 VDNEKTCVACLEEILQPCVLQCGHIFCHQCCYGALK--TVVHWCRTP 623
+D+ C+ C E P + C H FC C L+ + CR P
Sbjct: 23 LDSSLRCLICHEYFRAPLITSCSHTFCSFCIRDYLREHPMCPACRAP 69
>SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 456
Score = 29.9 bits (64), Expect = 0.32
Identities = 12/36 (33%), Positives = 15/36 (41%)
Frame = +3
Query: 507 CVACLEEILQPCVLQCGHIFCHQCCYGALKTVVHWC 614
C C +P L C H+FC C K V +C
Sbjct: 360 CAICSNVAYKPVRLGCSHVFCLHCLIILQKQKVDFC 395
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 28.3 bits (60), Expect = 0.97
Identities = 15/45 (33%), Positives = 19/45 (42%), Gaps = 3/45 (6%)
Frame = +3
Query: 477 IPNEVDNEKTCVACLEE---ILQPCVLQCGHIFCHQCCYGALKTV 602
+P E CV +E I P CGH +C+ C LK V
Sbjct: 185 VPQECGLCMMCVQRGDERVAITTPYTTDCGHTYCYACIMSRLKLV 229
>SPAC19A8.03 |||phosphatidylinositol-3-phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 559
Score = 27.9 bits (59), Expect = 1.3
Identities = 16/62 (25%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = -1
Query: 483 WEFLLIGPNVFSLSKHFVRSIQMHAVMLCSLVNL--NEHMKQLQQV*HEHNQCQSDFYLS 310
W+ + PN F ++ F+R + H + C + N ++ Q H +C D++LS
Sbjct: 452 WQIMDQFPNCFEFNERFLRRLLYH-LYSCQYGSFLYNSERERAQASVSTHTRCIWDYFLS 510
Query: 309 VK 304
K
Sbjct: 511 RK 512
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 27.1 bits (57), Expect = 2.3
Identities = 11/32 (34%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = +3
Query: 507 CVACLEEI-LQPCVLQCGHIFCHQCCYGALKT 599
C CLEE + + +CGH++C C ++T
Sbjct: 214 CPFCLEEKPVAARMSRCGHVYCFSCLLRFVET 245
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 26.2 bits (55), Expect = 3.9
Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
Frame = +3
Query: 507 CVACLEEILQPCVLQ-CGHIFCHQCCYGALKTVVHWC 614
C C E + ++ CGH FC+QC ++T C
Sbjct: 627 CSVCNFERWKDRIISLCGHGFCYQCIQKRIETRQRRC 663
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 25.8 bits (54), Expect = 5.2
Identities = 8/20 (40%), Positives = 15/20 (75%)
Frame = -1
Query: 540 KVVIFPPSMQHMSFRYPLHW 481
K++ + ++Q +SF +PLHW
Sbjct: 694 KLIEYDIALQPVSFHHPLHW 713
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 25.8 bits (54), Expect = 5.2
Identities = 15/49 (30%), Positives = 20/49 (40%), Gaps = 1/49 (2%)
Frame = +3
Query: 483 NEVD-NEKTCVACLEEILQPCVLQCGHIFCHQCCYGALKTVVHWCRTPF 626
NE + N C AC E ++ V CG+ CC C TP+
Sbjct: 175 NEYESNLYECNACREIVIAGYVCDCGYCLHVYCCKHLAHVNCINCNTPW 223
>SPAC13F5.06c |sec10||exocyst complex subunit
Sec10|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 25.8 bits (54), Expect = 5.2
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 3/73 (4%)
Frame = +3
Query: 81 KLPSYY-ARLASVLLSTFGENLT--RRFVNHVGKKIETNRSLRSEAQDLFLIAVKTLGDV 251
K+ Y+ A L S+++ G + R VN + I + D+F V LGD
Sbjct: 581 KIMGYFSAYLMSIVIPFTGVTASSRRETVNILSSSISV---IECAVNDVFYATVHALGDH 637
Query: 252 LPQIQSIHRALSY 290
L I S +R +SY
Sbjct: 638 LEIILSPYRQISY 650
>SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 25.8 bits (54), Expect = 5.2
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +3
Query: 33 LGEEYSGIVQVDDSYHKLPSYYARLASVLLSTFGENLTRRFVNH 164
+GE S ++V + +KLP Y A++ L T +L RR+V +
Sbjct: 247 IGEGASSFIRVINDRNKLPIYVAKVFRPPLDT---SLLRRYVRY 287
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 25.4 bits (53), Expect = 6.9
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = +1
Query: 505 HVLHAWRKYYNLVFFSVAIYSAINVAMAHLKQ 600
H +H W + ++ F VAI+ A+ +A L +
Sbjct: 2214 HNVHKWAMFLLVILFYVAIWIALVALLASLSR 2245
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 25.4 bits (53), Expect = 6.9
Identities = 11/29 (37%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +3
Query: 552 CGHIFCHQCC---YGALKTVVHWCRTPFT 629
CGH FC C Y + C TPF+
Sbjct: 655 CGHAFCSNCMEPFYEHKTSTCPQCETPFS 683
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 6.9
Identities = 18/55 (32%), Positives = 22/55 (40%), Gaps = 9/55 (16%)
Frame = +3
Query: 495 NEKTCVACLEEILQP-----CVLQCGHIFCHQCCYG-ALKTV---VHWCRTPFTK 632
N C C E++ +CGH+FC C KTV V CR TK
Sbjct: 143 NNIACAKCGNELVSDEKKSIFAAKCGHLFCSTCAKELRKKTVPCPVQHCRKRITK 197
>SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 247
Score = 25.4 bits (53), Expect = 6.9
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +1
Query: 511 LHAWRKYYNLVFFSVAIYSAINVAMAHLKQ 600
++ WRK +++ FFSV + A+ HL++
Sbjct: 100 INEWRKLFDINFFSVV--ETVKYAIPHLRK 127
>SPBC36B7.05c |||phosphatidylinositol|Schizosaccharomyces pombe|chr
2|||Manual
Length = 279
Score = 25.0 bits (52), Expect = 9.1
Identities = 8/18 (44%), Positives = 10/18 (55%)
Frame = +3
Query: 552 CGHIFCHQCCYGALKTVV 605
CG +FC+ CC K V
Sbjct: 52 CGKLFCYNCCNSFAKLPV 69
>SPAC227.09 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 25.0 bits (52), Expect = 9.1
Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Frame = -1
Query: 120 IIRLLNVR-SNLAVCGSYHLLGRFQSTLRLE 31
I+RL + S++ VCGS +LLG L+L+
Sbjct: 386 ILRLAKEKNSSVIVCGSLYLLGDMYRYLKLD 416
>SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 270
Score = 25.0 bits (52), Expect = 9.1
Identities = 9/34 (26%), Positives = 18/34 (52%)
Frame = -1
Query: 519 SMQHMSFRYPLHWEFLLIGPNVFSLSKHFVRSIQ 418
++QH FRY W + P + SL + + +++
Sbjct: 177 ALQHCGFRYNDKWAKRVKEPKLMSLMSYIITTVE 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,565,228
Number of Sequences: 5004
Number of extensions: 50851
Number of successful extensions: 164
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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